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36 results about "Alveolus pulmonis" patented technology

Method for separating and cultivating II-type epithelial cells of people alveolus pulmonis

The invention discloses a method for separating and cultivating II-type epithelial cells of people alveolus pulmonis. By using pancreatin joint elastase to digest and separate, the II-type epithelial cells of people alveolus pelmonis are obtained through filtering, differential adhension, density gradient centrifugation and the separation and purification of Anti-CD 14 magnetic beads. The invention establishes a set of method of separating, cultivating and identifying II-type epithelial cells of people alveolus pulmonis, and the maintaining situation of the biological characteristics of the cells cultivated by the method is assessed. The capacity of obtaining II-type epithelial cells of people alveolus pulmonis with high purity through the cultivating method is certified, moreover, the biological characteristics of cells can be enduringly maintained, and the method provides possibilities for further deep research of the proliferation, differentiation, liquid transferring and synthetizing secretion of II-type epithelial cells of alveolus pulmonis as well as the functional changes of II-type epithelial cells of alveolus pulmonis in the cases of pathophysiology, such as lung injury, lung repair.
Owner:GUANGZHOU INST OF RESPIRATORY DISEASE

Primer group and kit for rapidly identifying respiratory tract microorganisms based on nanopore sequencing and application of primer group

The invention discloses a primer group for detecting respiratory tract microorganisms based on a nanopore sequencing method. The nucleotide sequences of the primer group are as shown in SEQ ID NO:1-20. The microorganisms are streptococcus pneumoniae, staphylococcus aureus (resisting to methicillin), klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, stenotrophomonas maltophilia, candida albicans, haemophilus influenzae, legionella pneumophila, enterococcus faecium, chlamydia psittaci, cryptococcus gattii, aspergillus fumigatus and pneumocystis jiroveci. According to the invention, sequencing optimization is carried out on different types of samples of the respiratory tract microorganisms, so that the detection method, the kit and the like are suitable for various typesof respiratory tract samples; and a targeted amplification method is adopted, so that the detection requirements of sputum, alveolar lavage and other sample types can be met. In addition, parallel sequencing can be performed, so that the flux of the detected samples is increased, the sequencing time is shortened, and the contradiction between the flux of detected pathogenic species and the cost and time is further relieved.
Owner:GUANGZHOU DARUI BIOTECH

Lung benefiting pill with 25 ingredients having the function of being homology of medicine and food

The invention discloses a lung benefiting pill with 25 ingredients having the function of being homology of medicine and food. The composition of the lung benefiting pill comprises sea buckthorn, radix pseudostellariae, polyporus umbellatus, platycodon grandiflorum, stir-baked rhizoma dioscoreae, milk steamed poria cocos, longan aril, north astragalus, baked perilla seed, baked semen raphani, baked semen brassicae, radix glehniae, radix salviae miltiorrhizae, spina date seed, gecko tails, cordyceps sinensis, almonds, fructus schisandrae, linden honey, salted alisma orientalis, atractylodes macrocephala koidz baked with bran, angelica sinensis, milk steamed polygonatum kingianum, radix ophiopogonis, raw lily and the like. The lung benefiting pill is advantageous to guarantee normal ventilation of alveolus pulmonis and relieves organism anaerobic condition. Due to the fact that the permeability of lung blood capillary is improved, gas exchange is facilitated, and the symptoms of dyspnea and coughing with expectoration are relieved. Weak people with dyspnea and chronic cough generally has the abnormal phenomenon of hemorheology, synchronic treating of phlegm and blood stasis can solve the root cause of sputum and the root cause of blood stasis, bronchospasm can be relieved, discharging of sputum is promoted, cohesive force of red blood cells can be relieved, the blood viscosity is lowered, the hyperviscosity of the weak patients with dyspnea and chronic cough can be relieved, the cardio-pulmonary function of the patients can be improved, and therefore, the lung benefiting pill has good effect on treatment of the patients of this kind.
Owner:刘水平

Medicine for treating pig pneumonia

The invention discloses a medicine for treating pig pneumonia. The medicine is prepared from the following components in parts by weight: 16-38 parts of rhizoma decumbentis, 16-38 parts of spina gleditsiae, 16-38 parts of wild chrysanthemum flowers, 16-38 parts of viola, 16-38 parts of scutellaria baicalensis, 16-38 parts of platycodon grandiflorum, 16-38 parts of charred fructus crataegi, 16-38 parts of brown malts, 16-38 parts of prepared liquorice roots, 28-55 parts of radix angelicae dahuricae, 28-55 parts of akebia stems, 28-55 parts of tetrapanax papyriferus, 28-55 parts of radix paeoniae rubra, 28-55 parts of fructus trichosanthis, 28-55 parts of abrus herb, 28-55 parts of longhairy antenoron herb and 28-55 parts of plantain herb. The medicine can promote blood of pig lungs to flow and dredge bronchus, so that previous elasticity of alveolus pulmonis is recovered and the pig pneumonia can be effectively and rapidly treated; and the medicine is safe and has no toxic or side effect and no residues.
Owner:蒙焕文

Porcine alveolar macrophage healthy cell line and construction method and application

The invention discloses a healthy porcine alveolar macrophage cell line and a construction method and application. The collection number of the healthy porcine alveolar macrophage cell line is CGMCC No: 21498. According to the healthy porcine alveolar macrophage cell line, the 100% healthy porcine alveolar macrophage cell line is screened by adopting a special cell culture solution in combination with a natural domestication selection method, and the healthy porcine alveolar macrophage cell line can be applied to cell culture of viruses, research, development and production of live vaccines and inactivated vaccines and preparation of bioactive substances secreted by M1 and M2 macrophages after induced polarization, and the bioactive substances are used for cancer treatment, inflammation diminishing and the like. According to the invention, the healthy porcine alveolar macrophage cell line is cultured for the first time, any carcinogenic substances such as lentivirus transformation or plasmid transfection are not adopted in the construction method of the invention, and the constructed healthy porcine alveolar macrophage cell line keeps the characteristics of primary porcine alveolar macrophages.
Owner:BLUESKY BIOTECH (TAIZHOU) PARTNERSHIP (LLP)

Radiofrequency ablation volume reduction electrode capable of leading out waste gas in liver

The invention relates to a medical apparatus, in particular to a radiofrequency ablation volume reduction electrode capable of leading out waste gas in the liver. The radiofrequency ablation volume reduction electrode comprises a radiofrequency electrode tip, a temperature measurement resistor and a liver waste gas draw-out assembly. The radiofrequency electrode tip is connected with a radiofrequency transmission hole arranged in an outer pipe to give out a radiofrequency current, and is used for conducting radiofrequency ablation on lesion tissue of alveolus pulmonis. The temperature measurement resistor is connected with a temperature measurement resistor cable and used for collecting the temperature of an ablation area and regulating the temperature released by a radiofrequency electrode. The liver waste gas draw-out assembly comprises a gas draw-out hole and a gas draw-out passageway connected with the gas draw-out hole, and is used for drawing out waste gas in the liver. The radiofrequency ablation volume reduction electrode can conduct radiofrequency ablation treatment on the lesion tissue of the alveolus pulmonis and can meanwhile draw out a large amount of gas detained in the alveolus pulmonis which loses the function, and therefore the aims of reducing the volume of the liver and treating pulmonary emphysema are achieved.
Owner:王昌惠 +3

siRNA for silencing expression of Rongchang pig CD14 as well as screening method and application of siRNA

The invention discloses an siRNA for silencing the expression of a Rongchang pig CD14. Firstly, a porcine alveolar macrophage of a CD14-enhanced green fluorescent protein (EGFP) fusion protein for stably expressing a Rongchang pig CD14 gene is established; next, four siRNAs having different loci and targeting the CD 14 gene are synthesized; then, the four siRNAs are transfected into a CD14-EGFP stable cell line by applying an efficient transfection reagent; and finally, the siRNAs capable of efficiently inhibiting the expression of the Rongchang pig CD14 gene are screened, namely the siRNAs inan article 3 (669) are capable of obviously inhibiting the expression of the Rongchang pig CD14 gene, and therefore, a theoretical basis is provided for researches related to inflammatory diseases caused by treating endotoxin shock, endoxemia and gram-negative bacteria by targeting the Rongchang pig CD14 gene.
Owner:SOUTHWEST UNIVERSITY

A 25-flavor Yifei pill with the same origin of medicine and food

The invention discloses a lung benefiting pill with 25 ingredients having the function of being homology of medicine and food. The composition of the lung benefiting pill comprises sea buckthorn, radix pseudostellariae, polyporus umbellatus, platycodon grandiflorum, stir-baked rhizoma dioscoreae, milk steamed poria cocos, longan aril, north astragalus, baked perilla seed, baked semen raphani, baked semen brassicae, radix glehniae, radix salviae miltiorrhizae, spina date seed, gecko tails, cordyceps sinensis, almonds, fructus schisandrae, linden honey, salted alisma orientalis, atractylodes macrocephala koidz baked with bran, angelica sinensis, milk steamed polygonatum kingianum, radix ophiopogonis, raw lily and the like. The lung benefiting pill is advantageous to guarantee normal ventilation of alveolus pulmonis and relieves organism anaerobic condition. Due to the fact that the permeability of lung blood capillary is improved, gas exchange is facilitated, and the symptoms of dyspnea and coughing with expectoration are relieved. Weak people with dyspnea and chronic cough generally has the abnormal phenomenon of hemorheology, synchronic treating of phlegm and blood stasis can solve the root cause of sputum and the root cause of blood stasis, bronchospasm can be relieved, discharging of sputum is promoted, cohesive force of red blood cells can be relieved, the blood viscosity is lowered, the hyperviscosity of the weak patients with dyspnea and chronic cough can be relieved, the cardio-pulmonary function of the patients can be improved, and therefore, the lung benefiting pill has good effect on treatment of the patients of this kind.
Owner:刘水平

Radiofrequency ablation volume reduction electrode capable of exporting lung exhaust gas

The invention relates to a medical apparatus, in particular to a radiofrequency ablation volume reduction electrode capable of leading out waste gas in the liver. The radiofrequency ablation volume reduction electrode comprises a radiofrequency electrode tip, a temperature measurement resistor and a liver waste gas draw-out assembly. The radiofrequency electrode tip is connected with a radiofrequency transmission hole arranged in an outer pipe to give out a radiofrequency current, and is used for conducting radiofrequency ablation on lesion tissue of alveolus pulmonis. The temperature measurement resistor is connected with a temperature measurement resistor cable and used for collecting the temperature of an ablation area and regulating the temperature released by a radiofrequency electrode. The liver waste gas draw-out assembly comprises a gas draw-out hole and a gas draw-out passageway connected with the gas draw-out hole, and is used for drawing out waste gas in the liver. The radiofrequency ablation volume reduction electrode can conduct radiofrequency ablation treatment on the lesion tissue of the alveolus pulmonis and can meanwhile draw out a large amount of gas detained in the alveolus pulmonis which loses the function, and therefore the aims of reducing the volume of the liver and treating pulmonary emphysema are achieved.
Owner:王昌惠 +3
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