Method for screening early-stage lung tumor in assisting mode through bronchoalveolar lavage fluid
A technology for bronchoalveolar lavage fluid and lungs, which is applied in the field of medical testing, can solve the problems of high cost, high cost and trauma, and achieve the effect of accurate screening and low cost.
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Embodiment 1
[0042] like figure 1 As shown, this method of using alveolar lavage fluid to assist in screening early lung tumors includes the following steps:
[0043] (1) Use a fiberoptic bronchoscope to extend into the patient's lungs, and perfuse 37°C sterilized saline into the patient's lungs through the fiberoptic bronchoscope;
[0044] (2) Allow normal saline to stay in the patient's lungs for 3 to 5 minutes;
[0045] (3) By applying negative pressure on the outer end of the fiberoptic bronchoscope for negative pressure suction, the physiological saline injected into the patient's lungs is drawn out to form alveolar lavage fluid;
[0046] (4) Filter the alveolar lavage fluid with double-layer sterile gauze to remove the mucus in the alveolar lavage fluid;
[0047] (5) Treat the alveolar lavage fluid with an anticoagulant containing edetic acid;
[0048] (6) At room temperature, use a centrifuge at a speed of 1500r / min to centrifuge the alveolar lavage fluid for 10 minutes to furthe...
Embodiment 2
[0056] On the basis of Example 1, in step (10), the concentration P of M2-PK is also compared with the metabolic level of carcinoembryonic antigen CEA, and the normal range value of the metabolic level of carcinoembryonic antigen CEA is <10ug / L, if The concentration P of M2-PK is greater than 10ug / L, and the specific comparison method adopts the following steps:
[0057] (10-1) Use Pearson correlation analysis to analyze the correlation between the concentration P of M2-PK and the metabolic level of carcinoembryonic antigen CEA;
[0058] (10-2) Calculate the sensitivity, specificity, positive predictive value, negative predictive value and correct rate of M2-PK and carcinoembryonic antigen CEA respectively, and conduct comprehensive index evaluation, so as to judge whether the patient has early lung tumor auxiliary basis.
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