The invention relates to a pyropia haitanensis pure line
seedling cultivation method, and relates to pyropia haitanensis. According to the invention, the surface of the a single pyropia haitanensis
thallus is cleaned, and the pyropia haitanensis
thallus is dried in shade and is cryopreserved; the cryopreserved pyropia haitanensis
thallus is subjected to
resuscitation culture in still water under low light; a nutritive tissue block is soaked in a glucose solution; the soaked nutritive tissue block is
cut into small blocks, and the small blocks are subjected to enzymolysis in a conchase solution;
cell dissociation condition is observed; when free unicellular or multicellular masses from
algae fragments are observed,
cell sap is filtered; a filtrate is centrifuged, and a supernatant is discarded and a precipitate is retained;
centrifugation is repeated after washing; collected cells are subjected to dark culture in sterilized
seawater; cultivation is continued in a 1 / 2 MES culture solution; when the culture solution restores normal
seawater salinity, culturing is continued for 20-30 days; conchocelis is obtained by suction, such that pure line
germplasm conchocelis is obtained; still water culture is carried out for conserving the
breed; primary amplification, secondary amplification and third-level amplification are carried out, such that pure line free conchocelis are obtained; and the pure line free conchocelis is transplanted into seashells for carrying out
seedling cultivation.