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Phellodendron contrast extract and preparation method and application thereof

A Cortex Phellodendri extract and the technology of the extract are applied in the field of chemistry and can solve the problems of high detection cost and incomplete detection standards.

Inactive Publication Date: 2019-10-25
广州万正药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In order to solve the above-mentioned technical problems, the object of the present invention is to provide a kind of in order to solve the above-mentioned technical problems, the detection standard that the present invention exists in the Phellodendron cortex preparation or Phellodendron medicinal material detection is not comprehensive, the problem of using multiple reference substances to detect high cost, the present invention Provided is a Phellodendron cortex control extract that simultaneously detects compounds in Phellodendron phellodendron extracts and its preparations. The Phellodendron phellodendron control extract has good consistency, stable and uniform properties, and can be used for quality control of Phellodendron phellodendron and Phellodendron phellodendron prescriptions , qualitative and quantitative analysis and determination of effective compounds

Method used

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  • Phellodendron contrast extract and preparation method and application thereof
  • Phellodendron contrast extract and preparation method and application thereof
  • Phellodendron contrast extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Embodiment 1, the comparison test of Phellodendron Phellodendron reference extract of the present invention instead of reference medicinal material

[0084] Commercial medicinal materials were analyzed by thin-layer chromatography and high-performance liquid chromatography with chemical reference substance and Cortex Phellodendri extract as reference substances.

[0085] 1 TLC

[0086] 1.1 Sample preparation

[0087] Chemical reference solution: Accurately weigh berberine and prepare a 0.1mg / ml solution with methanol;

[0088]Phellodendron cortex reference extract solution: Accurately weigh the Phellodendron cortex extract reference substance prepared in Example 3, add methanol for ultrasonic extraction for 15 minutes, constant volume, prepare a solution with a concentration of 2.5 mg / ml, pass through a 0.45 μm filter membrane, Take the filtrate as the extract reference solution.

[0089] Medicinal material need testing solution: get Phellodendron Phellodendri medici...

Embodiment 2

[0127] The character analysis of embodiment 2 Cortex Phellodendron control extract

[0128] 1. Apparent state: the Phellodendron cortex contrast extract that embodiment 3 obtains is yellow powder.

[0129] 2. Moisture determination: Carry out according to Appendix IX G of the Chinese Pharmacopoeia of the 2010 edition (reduced pressure drying method). The detection result shows that the water content of the Cortex Phellodendri control extract is 5.41%.

[0130] 3. Consistency test: according to the method for embodiment 1, prepare 6 batches of Phellodendron cortex contrast extracts, after measuring each batch of berberine, the TLC detection result difference is very little; Detect its berberine through high performance liquid chromatography The contents are all within the standard range of deployment. Therefore, the consistency of the Cortex Phellodendri control extract prepared by the method for preparing the Cortex Phellodendron reference extract of the present invention is...

Embodiment 3

[0135] The preparation of embodiment 3 Cortex Phellodendri contrast extract

[0136] Instruments and reagents: Linomat 5 thin-layer chromatography semi-automatic spotting instrument, ATS 4 thin-layer chromatography automatic spotting instrument, thin-layer chromatography double-slot expansion cylinder, Chromatogram Immersion Device III chromatographic immersion tank and TLCvisualizer thin-layer chromatography camera instrument (Switzerland, CAMAG).

[0137] Agilent 1260series high performance liquid chromatograph equipped with DAD detector (AgilentTechnologies, USA).

[0138] Methanol, ethanol, and isopropanol were all analytically pure (Tianjin Damao Chemical Reagent Factory). Toluene, ethyl acetate, butyl acetate, concentrated ammonia and triethylamine were of analytical grade (Guangzhou Chemical Reagent Factory).

[0139] Berberine hydrochloride (also known as berberine), the marked purity is ≥98%, (Sichuan Weikeqi Biotechnology Co., Ltd.);

[0140] Medicinal materials i...

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Abstract

The invention discloses a Phellodendron contrast extract and a preparation method and application thereof. The extract is yellow dry powder, and comprises Phellodendron extract 67-84 wt% and adjuvant16-33 wt%, wherein the Phellodendron extract contains berberine at a content of not less than 30%. The Phellodendron contrast extract has good consistency, stable property, and uniformity, and can beused for quality control of Phellodendri medicinal material and Phellodendron preparation, and qualitative and quantitative analysis and determination of effective compounds.

Description

technical field [0001] The invention belongs to the field of chemistry, and in particular relates to a Phellodendron cortex reference extract and a preparation method thereof. Background technique [0002] Drug testing is an important means to verify the quality of drugs. With the widespread use of instrumental analysis, more and more drug reference materials are used. In drug inspection work, reference substances are often used as a special reference for checking the quality of drugs, and as a comparison standard for determining the authenticity of drugs. Reference substances play a very important role in drug testing. [0003] In the current standard of traditional Chinese medicine, the quality control mode of a single component is difficult to reflect the quality of traditional Chinese medicine, and the method of comprehensive quality control using multiple components or characteristic chromatographic peaks has attracted more and more attention. However, the shortage of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/90G01N30/02
CPCG01N30/02G01N30/06G01N30/90
Inventor 郭隆钢陆顺瑶许舜军许艺镌
Owner 广州万正药业有限公司
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