A kind of breeding method of high-yielding black-skinned chicken strain
A breeding method and technology of black-skinned chickens, applied in animal husbandry and other fields, can solve the problems of size influence of colony expansion, long cycle, cumbersome test cross process, etc., to achieve accelerated breeding process, high production performance, and overcome cumbersome test cross problems Effect
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[0030] The breeding method of high-yield type black-skinned chicken strain in the present embodiment, comprises the following steps:
[0031] (1) Using silky silkie pure line cock as the male parent and Bailai aircraft carrier chicken as the female parent to carry out crossbreeding to produce F1 generation chickens;
[0032] (2) The F1 generation is self-crossed to obtain the F2 generation, and the F2 generation black-skinned chicken is selected;
[0033] (3) Determine the relative copy number of the EDN3 gene of the F2 generation of black-skinned chickens by fluorescent quantitative PCR, and determine the genotype of the F2 generation of black-skinned chickens, specifically including the following steps:
[0034] ① Collection of samples
[0035] Take blood from the black-skinned chicken wings selected from the F2 generation, add 1 / 3 anticoagulant, and extract blood DNA by proteinase K method;
[0036] ② DNA sample concentration adjustment
[0037] Use a micro-spectrophotom...
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