Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fluorescent gold nano-cluster gel and preparation method thereof

A fluorescent nanometer and gold nanocluster technology, which is applied in the fields of chemistry and biological sciences, can solve the problems that the gel does not have fluorescent properties, and the color change cannot indicate the change of the pH value, etc., and achieves simple and easy operation, uniform gel properties, The effect of easy access to raw materials

Inactive Publication Date: 2016-06-08
HUBEI UNIV
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But existing gels are not fluorescent and cannot indicate changes in pH with a simple color change

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescent gold nano-cluster gel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0016] The second aspect of the present invention provides a method for preparing the above-mentioned fluorescent nano-gold cluster gel, the steps of which include:

[0017] A. At 50-80°C, add the gold nanoclusters to the polysaccharide aqueous solution, so that the volume percentage of the gold nanoclusters is 30-50%, and the volume percentage of the polysaccharide aqueous solution is 50-70%, and mix uniformly to obtain more gold nanoclusters. Polysaccharide mixed solution, the weight-to-volume ratio of polysaccharide to water in the polysaccharide aqueous solution is 0.1-2%;

[0018] B. The gold nanocluster polysaccharide mixed solution is left to stand, and washed with water to obtain a gold nanocluster hydrogel.

[0019] Preferably, the preparation method of the gold nanocluster comprises the following steps:

[0020] a, will contain Au 3+ The compound is added in BSA aqueous solution or DNA aqueous solution or glutathione aqueous solution, and by mass ratio, Au 3+ : BS...

Embodiment 1

[0028] (1) dissolving bovine serum albumin (BSA) in an aqueous solution to form a clear and transparent solution;

[0029] (2) the gold ion compound chloroauric acid according to Au 3+ : The weight ratio of BSA is 1:3 and added to the solution, and mixed evenly;

[0030] (3) under stirring, add sodium hydroxide to adjust the pH value of the solution to 12, and continue stirring overnight at a temperature of 50 degrees to obtain a gold nanocluster stock solution;

[0031] (4) precipitating the gold nanocluster stock solution, and redispersing the purified gold nanocluster solution;

[0032] (5) Add 0.4% (w / v) agarose into deionized water, that is, add 0.4g agarose per 100ml of deionized water, heat to make it completely dissolve to form a polysaccharide solution, and stop heating;

[0033] (6) After the polysaccharide solution is cooled to 60° C., add the gold nanocluster solution to the polysaccharide solution according to the volume ratio of 0.5:1, and stir evenly to prepar...

Embodiment 2

[0038] (1) Dissolving DNA in an aqueous solution to form a clear and transparent solution;

[0039] (2) the gold ion compound chloroauric acid according to Au 3+ : DNA weight ratio of 1:4 is added to the solution and mixed evenly;

[0040] (3) Under stirring, add sodium hydroxide to adjust the pH value of the solution to 11, and continue stirring overnight at a temperature of 37° C. to obtain a gold nanocluster stock solution;

[0041] (4) precipitating the gold nanocluster stock solution, and redispersing the purified gold nanocluster solution;

[0042] (5) Mix agarose, sodium alginate and gelatin according to the weight ratio of 7:2:1, and then add 0.7% (w / v) of the mixed powder into deionized water, that is, add 0.7% per 100ml of deionized water g mix powder, heat, make it dissolve completely and form polysaccharide solution, stop heating;

[0043] (6) After the polysaccharide solution is cooled to 65° C., add the gold nanocluster solution to the polysaccharide solution ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides fluorescent gold nano-cluster gel. The gel comprises components in percentage by volume as follows: 30%-50% of gold nano-clusters and 50%-70% of a polysaccharide aqueous solution, wherein the weight / volume ratio of polysaccharides to water in the polysaccharide aqueous solution is 0.1%-2%. A preparation method of the gel comprises steps as follows: A, the gold nano-clusters are added to the polysaccharide aqueous solution at 50-80 DEG C, the volume percentage of the gold nano-clusters is 30%-50%, the volume percentage of the polysaccharide aqueous solution is 50%-70%, a gold nano-cluster and polysaccharide mixed solution is obtained after even mixing, and the weight / volume ratio of the polysaccharides to the water in the polysaccharide aqueous solution is 0.1%-2%; B, the gold nano-cluster and polysaccharide mixed solution is left to stand and washed with water, and the gold nano-cluster hydrogel is obtained. The gold nano-cluster hydrogel is very sensitive to pH, indicates change of the pH value through simple color change, has the wide pH value indicating range and is more uniform in gel property, and the detection result is accurate and good in reproducibility; raw materials are easy to obtain, low in cost and wide in source, the preparation process is pollution-free, operation is simple and easy to implement, and the gel is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the fields of chemistry and biological sciences, and relates to a kind of fluorescent nano-gold cluster gel sensitive to pH and a preparation method thereof. Background technique [0002] In modern chemistry, agriculture, biology and environmental science, the measurement of pH value is particularly important. To measure the change of pH value, a membrane glass electrode sensitive to pH value is generally used. However, the traditional glass electrode has some disadvantages, such as bulky, requires a large amount of liquid, and the glass is easy to break, which limits its application. Fabrication of new pH sensors with different materials has aroused research interest. [0003] At present, there are related technologies of nanogels for measuring pH, but most of them are directly prepared into nanogels by using high molecular polymer materials. For example, there is a technology that uses polystyrene / polyacrylic acid core-she...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/80G01N21/64
CPCG01N21/6428G01N21/80
Inventor 熊华玉王薇梁继超王升富文为张修华郑慧铃
Owner HUBEI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com