The invention discloses a tree peony bark-containing shampoo capable of blackening and nourishing hair. The tree peony bark-containing shampoo capable of blackening and nourishing hair is prepared from a raw material drug and an accessory ingredient; the raw material drug is composed of, by weight, 10 to 30 parts of tree peony bark, 5 to 15 parts of polygonum multiflorum, 8 to 12 parts of fructusxanthii, 5 to 15 parts of cacumen biotae, 5 to 15 parts of mulberry, 8 to 12 parts of broadleaf holly leaf, 8 to 12 parts of Chinese honey locust, 1 to 9 parts of radix scutellariae, 6 to 14 parts ofglossy privet fruit, 8 to 12 parts of indigo naturalis, 9 to 18 parts of black soya bean, and 2 to 20 parts of honey; the accessory ingredient is composed of beta-cyclodextrin and maltodextrin. The tree peony bark-containing shampoo is prepared based on a pure traditional Chinese medicine natural formula; tree peony bark, polygonum multiflorum, and mulberry are used for nourishing blood and moisturizing hair; cacumen biotae, broadleaf holly leaf, glossy privet fruit, and black soya bean are used for dispelling wind and activating blood circulation, clearing and activating the channels and collaterals, and activating hair follicles; combination of the monarch drug and the ministerial drug, and the synergistic interaction effect of the traditional Chinese medicines are capable of improving scalp environment completely, clearing stain and excess grease in hair follicles, activating blocked hair follicles, ensuring absorption of nutrients; no side effect is caused; and no chemical residueis left.