54results about How to "Simple detection equipment" patented technology

The invention discloses a horizontal visual angle estimation and calibration method based on a depth camera, comprising steps of adopting a Kinect depth camera to capture a human face 2D image and an estimation head posture, ;positioning an optimal pupil center point in a the2D image to obtain the 2D coordinate of the optimal pupil center point, ;using a fixed an intrinsic parameter of a Kinect depth camera to convert the 2D coordinator of the optimal pupil center point obtained from the step 2 to a 3D coordinate system of the Kinect depth camera to obtain the 3D coordinator of the optimal pupil center point, ;using the 3D eyeball model to estimate the horizontal sight line angle, ;and designing a test system and obtaining the sight line angle through calibration. The method is simple in operation, good in instantaneity, and low in cost and has low limitation on the head of the tester and has the high accuracy.

The invention belongs to the field of preparation and application of nano materials, and discloses a composite nano material capable of exciting upconversion steady persistence through near-infrared light, and preparation method and application of the composite nano material. By utilizing the composite nano material disclosed by the invention, the situation that nanoparticles are excited by near-infrared wavelength light to generate steady persistence luminescence is realized. The composite nano material is of a special composition structure which is obtained through combination of an upconversion nano material and a steady persistence nano material. According to the composite nano material disclosed by the invention, the situation that visual light emitted the upconverision nano material after near-infrared light is absorbed by the upconversion nano material is matched with absorbed light of the steady persistence nano material, i.e., the visual light emitted by the upconversion nano material is absorbed by the steady persistence nano material, so that light emitting of the steady persistence nano material is realized, the generation of steady persistence luminescence under the exciting of the near-infrared light is realized, the problems of a traditional steady persistence nano probe that the penetrating power of short-wavelength laser is weak, materials in a living organism cannot be excited, and the like are overcome, and deep optical imaging with continuous detection, high SNR (Signal to Noise Ratio) and a simple detecting device and without background exciting light, tissue autofluorescence, and a light filter is realized.

The invention discloses an enclosed bus installing device and method. The enclosed bus installing device comprises a laser lamp and a reflector, wherein the laser lamp is detachably connected to one enclosed bus shell, the reflector is arranged on the other enclosed bus shell. The two shells are each provided with an adjusting mechanism. Each adjusting mechanism comprises a horizontal adjusting mechanism and a vertical adjusting mechanism. Each horizontal adjusting mechanism comprises a base connected to the ground, wherein a sliding block is connected to the base in a sliding manner, the baseis provided with a sliding mechanism driving the sliding block to move, and the sliding mechanism is provided with a locking mechanism capable of limiting movement of the sliding block. Each verticaladjusting mechanism is connected to the corresponding sliding block, an arc plate is fixed to the vertical adjusting mechanism, the vertical adjusting mechanism can drive the arc plate to move in thevertical direction, and the axis of the arc plate and the movement direction of the sliding block are perpendicular. A supporting mechanism is arranged below the ends, close to each other, of the twoshells. The enclosed bus installing device has the effect of conveniently and rapidly adjusting the coaxiality between two enclosed buses.

The invention discloses an initial alignment method of a permanent magnetic synchronous motor rotor of a pure electric vehicle, comprising the following steps: 1, respectively detecting a deviation amount of a permanent magnetic synchronous motor between a zero position of a rotary transformer mounted on a motor axle and a mechanical zero position of the motor at six level states; 2, judging the deviation amount in the first step: if the deviation amount is smaller than the preset error, calculating an average value of the six deviation values as an initial position of the motor rotor; if the deviation amount is larger than the preset error, detecting an outlet sequence of the permanent magnetic synchronous motor and then repeating the above steps. The initial alignment method has the following advantages: 2, the detection method is simple and practical; 2 during the detection process, the motor is detected without being mounted on a testing stand; 3, the detection device is simple, a switch and a contactor set are needed and a frequency converter and a motor controller are not needed to detect; 4, the detection accuracy is high, the maximum initial alignment error by the method does not exceed +- 5 degree electrical angles; 5, the outlet sequence of the motor is detected by a certain logical relationship judgment.

The invention belongs to the field of chemical detection, relates to a visualized and rapid detection method for detecting residual amount of benzothiostrobin in strawberries, and relates to pesticideresidue detection, in particular to a quantum dot-labeled antibody probe test strip as well as a preparation method and application thereof. The quantum dot-labeled antibody probe test strip comprises a bottom plate, a nitrocellulose membrane, a sample pad, a bonding pad and an absorption pad, wherein the nitrocellulose membrane is located on the bottom plate; the sample pad and the bonding pad are sequentially arranged on the bottom plate on the left side of the nitrocellulose membrane, and the absorption pad is arranged on the bottom plate on the right side of the nitrocellulose membrane; quantum dot-labeled antibody probes are distributed on the bonding pad; and benzothiostrobin antigens and goat-anti-mouse antibodies are further distributed on the nitrocellulose membrane. The invention provides the visualized and rapid detection test strip which is suitable for quantitatively detecting the residual amount of the benzothiostrobin in strawberries.

The present invention discloses dynamic detector and detecting method for astigmatism of crystalline lens. Dynamic pictures with changeable colors and patterns and astigmatism pattern for astigmatism measurement are shown on the screen and dynamic astigmatism of crystalline lens is induced through the observation of eyes to the screen. By means of observing the astigmatism pattern and matching with the detection with slit lens, the dynamic astigmatism of crystalline lens for different colors and different distances are detected. The detector of the present invention can detect the dynamic astigmatism of crystalline lens accurately, and the detection method is simple in operation. The present invention is especially suitable for detecting the corrected diopter of computer operator.

The invention discloses a ratio fluorescent probe, a fluorescent paper chip and a detection method for detecting mercury ions, the ratio fluorescent probe comprises a background probe and a reaction probe, the background probe is composed of nitrogen-doped blue carbon quantum dots, the reaction probe is composed of red gold nanoclusters, the ratiometric fluorescent probe is formed by mixing red gold nanoclusters and nitrogen-doped blue carbon quantum dots, after mercury ions are added into the ratiometric fluorescent probe, the red fluorescence of the gold nanoclusters is gradually quenched, and the fluorescence of the nitrogen-doped blue carbon quantum dots is kept unchanged and is used as a reference signal; and therefore, the On-Off type fluorescent probe is formed. The two fluorescent materials of the ratiometric fluorescent probe are easy to prepare, and the ratiometric fluorescent probe has the characteristics of low toxicity, high fluorescence intensity, good biocompatibility and the like, has a wider color change range, and is more beneficial to detection of mercury ions. When the probe is fixed on a paper chip sensor, rapid visual detection of mercury ions can be realized.

This invention discloses a protein chip test method through colloidal gold and silver strengthening method, which comprises the following steps: a, making the protein chip react with the sample to form the first compound; b, making the chip react with the first identifying molecule of first compound to form second compound; c, mixing the chip with colloidal gold solution of the second coupled pair part to form the second compound and third compound composed of colloidal gold; d, mixing the chip with silver strengthening solution to make the silver ions form metal silver under function of gold catalyzing; e, stopping silver strengthening reaction and measuring the gray degrees of each point sample area.

The invention discloses an RNA in-situ hybridization (ISH) kit for detecting overexpression of human epidermal growth factor receptor (EGFR) 2 (HER2) genes of breast cancer patients and belongs to the field of molecular pathological diagnosis. The kit comprises stationary solution, PBS-BufferI, PBS-BufferII, PBS-BufferIII, digestive juice, prehybridization solution, hybridization solution, Washing BufferI, Washing BufferII, buffer solution I, buffer solution II, blocking liquid, development solution I, development solution II, and a positive control tissue glass plate. Reference basis is provided for diagnosing positive breast cancer patients and important clinical value for early diagnosis of breast cancer and formulation of personalized medicines is provided by detecting the expression level of HER2.

The invention discloses a hydrogen sulfide early-warning measuring method which is characterized by comprising the following steps: using a sensor to detect the contents of sulphur ions and hydrogen ions in drilling fluid and the temperature of the drilling fluid; calculating the content of hydrogen sulfide in the drilling fluid based on the detected data, ionization equilibrium and saturation solubility properties; comparing with the saturation solubility of a hydrogen sulfide solution; and sending out an alarm in time before hydrogen sulfide gas overflows out of the drilling fluid. Compared with the prior art, the early-warning measuring method has the advantages of simple equipment, convenient operation and high early warning accuracy, thereby greatly avoiding personal safety accidents. Thus, the early-warning measuring method has an important significance in preventing hydrogen sulfide overflowing accidents in petroleum drilling industry.

The invention relates to an airtightness detection system of a civil air defense door. The system comprises sealing cabin, a control system installed in the sealing cabin and a to-be-detected civil air defense door arranged in the sealing cabin. The control system comprises a pressure increasing mechanism, a pressure reducing mechanism, a smog spraying mechanism, a leakage measurement device and a vacuumizing mechanism. The pressure increasing mechanism, the pressure reducing mechanism, the smog spraying mechanism, the leakage measurement device and the vacuumizing mechanism are installed in the sealing cabin. A human-machine interface is arranged on the side end of the sealing cabin. The control system is connected with the human-machine interface. The smog spraying mechanism is a high pressure smog sprayer and sprays colored smog body. The interior of the sealing cabin is a dust-free environment. A civil air defense door installation rack is installed in the sealing cabin. According to the invention, airtightness of the civil air defense door can be effectively and simply measured; positions of air holes can be positioned; convenience is brought for repair; and the detection device is simple, low in cost and suitable for promotion and use.

The invention discloses an automobile wheel hub dynamic balance detection tooling, which comprises a horizontal work table, a rotating disc, a detection device and a motor, wherein the upper end surface of the horizontal work table is connected with the rotating disc through a connecting post; a plurality of detection devices are fixed on the horizontal work table; the detection device is arrangedaround the rotating disc; one end of the connecting post is rotationally connected with the horizontal work table through a bearing; the other end of the connecting post is connected with a fixing disc; the fixing disc is fixedly installed with the bottom surface of the rotting disc through screw bolts; a motor is arranged above the rotating disc; the motor is connected with a telescopic air cylinder; the detection device comprises a detection support frame and an electronic dial indicator. During the wheel hub dimension detection, the rotation is stable; the accuracy of the detection resultcan be effectively ensured; meanwhile, the tooling can adapt to the wheel hubs in various dimensions and models; the detection cost and the detection time can be reduced; the measurement mode is simple; the structure is simpler relative to the detection equipment in the prior art.

The invention discloses a Mn and Co co-promoted cobaltosic oxide / silicon dioxide catalyst for removing non-methane total hydrocarbon in total hydrocarbon as well as preparation and application of theMn and Co co-promoted cobaltosic oxide / silicon dioxide catalyst. The catalyst mainly comprises an active component Mn-modified Co3O4 and a Co-modified carrier SiO2, the loading capacity of the activecomponent is 20-30%, the molar ratio of Mn to Co is 1: 10 to 1: 20, and the molar ratio n (Co): n (Si) of Co to Si in the Co-modified SiO2 carrier is 1: 20 to 1: 30. The catalyst is synthesized by twosteps of a hydrolysis method and a coprecipitation method. The modified Co-promoted SiO2 carrier has stronger binding force with the active component, and Mn is introduced into Co3O4, so that the activity and stability of the catalyst are further improved. The preparation method of the catalyst is simple, the temperature of an operation window is low, and the catalyst has very high practical application value for removing non-methane total hydrocarbons in total hydrocarbons.

The invention discloses a resonance light scattering detection method for sodium heparin, wherein under an alkaline condition with the NaOH concentration being 0.042mol / L, Co(II) and a cobalt reagent are bonded to produce a complex with positive charges, the sodium heparin carries negative charges and is electrostatically bonded with the complex to form a ternary complex, so that the intensity of resonance light scattering is enhanced obviously and has the maximal scattering peak located at 550nm, the scattering intensity is in good linear relation with the concentration of the sodium heparin in a range of 0.05-1.60 mu g / mL, and the detection limit (3 sigma) is 1.3ng / mL. The method has the advantages of simple required detection equipment, readily available reagent, simpleness for operation, short detection time, wider linear range and higher sensitivity. The invention enriches the detection methods of the sodium heparin and is favorable for meeting detection requirements under different conditions.

The invention relates to a method for detecting a gene polymorphism SNP (single nucleotide polymorphism) site by utilizing cyclization of RNase H (ribonuclease H), which belongs to the technical field of molecular biology. Transpeptidase Sortase A is utilized for cyclization of the linear RNase H, and a probe in the loop-stem structure, a DNA (deoxyribonucleic acid) fragment to be detected, a forward primer and a reverse primer of DNA specificity to be detected, a Vent DNA polymerase and cyclized APERNase H II are further utilized for measuring the specific site SNP of the DNA fragment to be detected, thereby improving the heat stability of the RNase H.

The invention discloses a method for detecting bending mechanical property of a test sample. The method for detecting the bending mechanical property of the test sample comprises the following steps of S1, selecting a plurality of groups of test samples; and moreover, correspondingly marking the test samples as A1 to An in sequence; S2, placing the test sample with the mark of A1 on a support, andusing a fixture to fix the two ends of the test sample A1 on the support; S3, applying a pressure to the test sample A1 by using a piston rod of a hydraulic cylinder, recording lengths H1 from the bent arc top of the test sample A1 to horizontal positions at the two ends of the test sample A1, and recording the pressure of the hydraulic cylinder as F1; S4, repeating the steps S1 to S3 till all detection of the test samples A1 to An is finished, and obtaining two groups of data H1-Hn and F1-Fn; and S5, making a plane rectangular coordinate graph with the pressure F as a horizontal coordinate and the length H as a vertical coordinate in a plane rectangular coordinate system, and then, drawing a mean value of the graph according to the plane rectangular coordinate graph. The method for detecting the bending mechanical property of the test sample, disclosed by the invention, has the advantages that the detection method is simple, the operability is high, and the bending forming performance of the test sample can be more comprehensively and accurately tested.

The invention builds an enzyme biofuel cell (EBFC)-based self-powered bacteria biological sensor and application thereof, and belongs to the technical field of biological sensors. Electrons from a biological positive electrode can be received by prussian blue (PB) and can be converted to prussian white (PW), blue of the PB also can be converted to transparent PW by obtaining the electrons, the changing speed from blue to nature is dependent on concentration of vibrio parahaemolyticus, and the color can be restored to an original PB state by connecting the color to the biological positive electrode. When target vibrio parahaemolyticus exists, an open-circuit voltage generated by the EBFCs is reduced accordingly, the amplitude of the open-circuit voltage is dependent on the concentration ofthe vibrio parahaemolyticus identified by a target adaptor. Due to a steric-hinerance effect of the vibrio parahaemolyticus, fuel glucose is prevented from being approaching an active site of glucosedehydrogenase on the positive electrode, and the color of the prussian blue is reduced. By the EBFC-based self-powered bacterial biological sensor, simple, convenient, rapid, sensitive and high-efficiency detection of food pathogens is achieved.

The invention discloses a method and apparatus for predicting fatigue life of concrete based on Weibull equation and residual deformation. In the developing field of modern civil engineering, the fatigue behavior of concrete has become one of the focuses of attention. How to accurately predict the fatigue life of concrete has become an important problem in the field of engineering construction. The method and device provided by the invention can be used for life prediction of concrete under fatigue load and characterization of fatigue deformation evolution law. The invention has the advantagesof simple steps, easy use and high precision. The calculation amount can be greatly reduced in use, and only two fatigue parameters, the number of fatigue load cycles n and the residual deformation Epsilon p of the nth cycle, can be measured, which simplifies the testing equipment. The model can provide important technical support for the whole process of engineering design, construction, testingand maintenance.

The invention relates to a rapid and sensitive plasmodium falciparum detection method. The detection method integrates two powerful molecular biology techniques of a polymerase chain reaction PCR and a microarray, PCR hybridized probes are directly fixed on a hybridization cabin in the microarray and are on a same chip with a PCR reaction chamber; the detection method includes the steps of extracting a blood sample DNA liquid, carrying out PCR amplification, hybridizing, cleaning, and reading and discriminating a result. The method can quickly and sensitively detect plasmodium falciparum, can greatly improve the detection efficiency of front line inspection and quarantine personnel of import and export ports, not only can reduce the workload, but also furthest solves a positive detection missing problem possibly existing in a traditional detection method, so as to furthest prevent generation of plasmodium epidemic situation.

The invention aims to provide a method for determining parathion in grain by combining dispersive liquid-liquid microextraction with digital image colorimetry, belongs to the technical field of food safety detection, and adopts hydrophilic DES as an extraction agent to replace traditional toxic organic solvents such as methanol and acetonitrile to complete extraction of parathion pesticide in grain samples. Hydrophobic DES synthesized by a hydrogen bond acceptor and a hydrogen bond donor according to a certain proportion can be dissociated into TBA < + > and alcohol in situ, parathion in a hydrophilic DES phase is converted into a yellow product by the dissociated hydrophilic TBA < + > under an alkaline condition, and the yellow product is further extracted and purified by a hydrophobic extraction agent alcohol which is generated in situ and dispersed in a solution. And then performing quantitative detection on yellow products in the organic phase by using a digital image colorimetric method. The whole process is simple to operate, low in cost, rapid and sensitive, and has the advantages of avoiding use of organic solvents, saving the dosage of extracting agents, being green and environment-friendly and the like.

The chip is composed of two pieces of basis materials being bonded. The mini pump without valve, the in and out flow channels as well as intercrossed the electrophoresis separation channel and the electrophoresist sample channel are setup in the adjoining plane. The one end of the electrophoresis sample channel and the in flow channels are connected each other in 'T' form. The mini pump without valve is utilized as the automatic sample change unit in electrophoresist continuous measuring multiple samples. With having been measured, the former sample is discharged to the waste tank. Meanwhile the new sample is fed to the sample introduction point. The chip can be repeatable use so as to simplify the test device, shorten the continuous measuring time for multiple samples, lower the operation requirements.

The invention provides a detection kit and a detection method for dibutyl phthalate in edible oil and fatty foods. The detection kit comprises a dibutyl phthalate standard solution, a solid phase carrier coated with an antigen, an anti-reagent, an enzyme marker, a color developing solution, a stop solution, a concentrated washing solution, a complex solution, and a purifying agent. The detection method comprises a sample preparation step and a detection step. The kit and the method can directly detect the dibutyl phthalate in the edible oil and the fatty foods, and has the advantages of simpledetection device, high detection sensitivity, low cost, convenient operation, and convenient carrying, so that the industrial production can be realized.

The invention discloses a method for quantitatively detecting mutant DNA by combining GQDs-based resonance light detection probes with CHA technology. The invention firstly synthesizes graphene quantum dots, then designs two kinds of hairpin probes H1 and H2 for the target object, and then prepares two kinds of probes GQDs‑H1 and GQDs‑H2 through condensation reaction of the probes and GQDs. In the PBS buffer solution reaction system, when p53 mutant DNA is added, the aggregation of GQDs-H1 and GQDs-H2 will be caused, and a significantly enhanced resonance light signal will be generated to realize the quantitative detection of p53 mutant DNA. The invention has high sensitivity and selectivity, the linear range is 1pM to 50nM, the detection limit is 0.8pM, and the target object can be quantitatively detected effectively.

The invention discloses a reagent kit and a method for rapidly, qualitatively and quantitatively detecting microcystic aeruginosa on spot, detecting by using a nucleic acid probe with a fluorescein isothiocyanate added at the 5'end. The detection method comprises the following steps: firstly fixing algae cells; dehydrating and hybridizing, and cleaning and observing the algae cells under a fluorescence microscope.The invention has the advantages of correctness, rapidness and simpleness, and has simple operation because less than 20 hours is required for the whole detection process. The most important is that detection can be performed on spot, and qualitative analysis of the algae cells can be carried out in natural water body, which can not be achieved by the prior other methods for authenticating microcystis flos-aquae, therefore, the invention has favorable application prospect on detection of the microcystis flos-aquae.

The invention relates to the field of dielectric ceramic lattice defect detection of ceramic elements and discloses a method for indirectly detecting dielectric ceramic lattice defects of an MLCC. The method comprises the following steps of preparing materials, preparing a test fixture, a high-temperature aging oven, an ampere meter, a universal meter and an insulation tester according to requirements; mounting the to-be-tested products, mounting the to-be-tested products on the test fixture in series; state confirmation, confirming that an installation state of the to-be-tested product is good; adjusting test conditions, putting a to-be-tested product into a high-temperature aging oven, and presetting a test temperature and a test voltage; setting value taking time, setting a current acquisition value taking time interval; starting a test, starting a high-temperature aging oven, and enabling the aging oven to be in a heat preservation state for testing after the temperature reaches a preset value; timing value taking, taking a value of the current of the to-be-tested object according to a preset time interval; and calculating an insulation resistance value at each moment. Through the mode, lattice defects of the MLCC dielectric ceramic can be effectively and indirectly detected, and operation is simple and visual.

The invention relates to a rapid and high-throughput plasmodium detection method. According to the method disclosed by the invention, two powerful molecular biological technologies of polymerase chain reaction (PCR) and micro-array are integrated, a PCR hybridization probe is directly fixed in a hybridization cabin and arranged on the same chip as a PCR reaction chamber; the detection method comprises the steps of blood sample DNA liquid extraction, PCR amplification, hybridization, washing and result analysis. The rapid and high-throughput plasmodium detection method disclosed by the invention can be used for rapid sensitive detection of falciparum malaria plasmodium, vivax malaria plasmodium and quartan malaria plasmodium, the detection efficiency of front-line inspection and quarantine personnel at import-export ports is greatly improved, not only is the workload reduced but also the problem of positive undetected samples possibly missed according to the conventional detection methods can be solved to the largest extent, and therefore the occurrence of plasmodium epidemic situation is prevented to the largest extent.

The invention discloses a concrete fatigue deformation evolution model based on Weibull equation. In the developing field of modern civil engineering, the fatigue behavior of concrete has become one of the focuses of attention. How to accurately characterize the fatigue evolution of concrete and predict the fatigue life of concrete has become an important issue in the field of engineering construction. The model provided by the invention can be used for characterizing the deformation evolution law of concrete under compression, tensile and flexural fatigue loads. It has many advantages such asapplicable load forms, concise expression, easy to use and high precision. The calculation amount can be greatly reduced in use, and only two fatigue parameters, the number of fatigue load cycles n and the deformation Epsilon corresponding to a certain stress in the nth cycle, can be measured, which simplifies the testing equipment. The model can provide important technical support for the wholeprocess of engineering design, construction, testing and maintenance.

On surface of substrate B, following structure are prepared: buffer solution groove, spent solution groove of electrophoretic separation pipe, sample introduction pipe and spent solution groove, flow channel of minitype magnetic pump and sample introduction groove. Electrodes are spluttered on two walls of whole flow channel. Sample introduction groove, buffer solution groove, two spent solution grooves and tapping point of electrode on substrate B are aligned to and bonded to relevant exits of substrate A. permanent magnet is installed below electrode of substrate B. minitype magnetic pump provides and changes multiple samples for continuous electrophoresis test. When a sample test is finished, former sample is discharged to spent solution groove, and new sample is delivered to separation point. The invention shortens time of continuous test and is available in repeatable use.

The invention discloses a device and method for acquiring the vertical incident direction of sunshine. The method comprises the steps of collecting sun illumination signals by virtue of a photosensitive diode integrated sensor, processing the collected signals by virtue of an LM339N chip, carrying out logical operation by virtue of STC90C516RD+, and finally acquiring the vertical incident direction of the sunshine, wherein the photosensitive diode integrated sensor is produced by embedding a plurality of photosensitive diodes into a black hemisphere shell. According to the technical scheme, the vertical incident direction of the output sunshine is represented by a direction of a spatial vector gamma (-x, -y, -z) with modulus of 1. The design scheme has the beneficial effects that the cost is low, the system design is simple, and the stability is relatively strong; and by increasing the number of the photosensitive diodes embedded into the photosensitive diode integrated sensor, the requirement on the detection precision of the vertical incident direction of the sunshine can be met. According to the design scheme, a sun tracking system with changeable tracking precision can be produced.

The invention belongs to the field of preparation and application of nano materials, and discloses a composite nano material capable of exciting upconversion steady persistence through near-infrared light, and preparation method and application of the composite nano material. By utilizing the composite nano material disclosed by the invention, the situation that nanoparticles are excited by near-infrared wavelength light to generate steady persistence luminescence is realized. The composite nano material is of a special composition structure which is obtained through combination of an upconversion nano material and a steady persistence nano material. According to the composite nano material disclosed by the invention, the situation that visual light emitted the upconverision nano material after near-infrared light is absorbed by the upconversion nano material is matched with absorbed light of the steady persistence nano material, i.e., the visual light emitted by the upconversion nano material is absorbed by the steady persistence nano material, so that light emitting of the steady persistence nano material is realized, the generation of steady persistence luminescence under the exciting of the near-infrared light is realized, the problems of a traditional steady persistence nano probe that the penetrating power of short-wavelength laser is weak, materials in a living organism cannot be excited, and the like are overcome, and deep optical imaging with continuous detection, high SNR (Signal to Noise Ratio) and a simple detecting device and without background exciting light, tissue autofluorescence, and a light filter is realized.