173results about How to "Does not affect test results" patented technology

The invention discloses a method for detecting the damage defect of a curved tag based on template matching and similarity calculation, and belongs to the technical field of machine vision and video image processing. The method comprises steps of image acquisition, image preprocessing, template area extraction, defect detection and result display. The image acquisition comprises taking different images of a tag to be tested and a template tag from three angles. The preprocessing process realizes the segmentation of a tag area and an image processing operation for converting a curved surface into a flat surface. The template area extraction achieves one-to-one correspondence between the images of the tag to be tested taken from three different angles and a certain area of a template panorama, enables a bottle rotation angle in image extraction not to be restricted, and reduces system implementation difficulty. A defect detection module, based on a method combining the template matching with the similarity calculation, locks possible defective areas by using short time-consuming template matching, determines whether the defect exists by using exact similarity calculation, and improves detection efficiency on the basis of guaranteeing a detection effect.

The invention provides a real-time fluorescence quantification PCR method with magnetic bead nucleic acid extraction and amplification conducted in one tube. A lysis solution mixed with magnetic beads and a sample to be detected are added to a PCR amplification tube, even mixing and standing are conducted, mixed liquor is extracted after magnetic extraction, and obtained magnetic beads are washed once; a well prepared PCR liquid is added into the PCR amplification tube for real-time fluorescence quantification PCR of target nucleic acid; the lysis solution is prepared from 0.2-0.4N sodium hydroxide, 0.3-0.6M potassium chloride, 0.01-0.05%N-sodium lauroyl sarcosine, 5mM EDTA, 0.3-0.6M Tris-HCL, and 1-2% Triton X-100. According to the method, heating is not needed, indoor-temperature pyrolysis is conducted for about 5-10 min only, static washing is needed only once, and then laboratory pollution and magnetic bead nucleic acid loss are reduced, pollution possibility brought by step-by-step operation is avoided and detection time is shortened. By taking HBV DNA quantification as an example, sensitivity can be as low as 5 IU / ML, and repeatability is high.

The invention discloses a horizontal visual angle estimation and calibration method based on a depth camera, comprising steps of adopting a Kinect depth camera to capture a human face 2D image and an estimation head posture, ;positioning an optimal pupil center point in a the2D image to obtain the 2D coordinate of the optimal pupil center point, ;using a fixed an intrinsic parameter of a Kinect depth camera to convert the 2D coordinator of the optimal pupil center point obtained from the step 2 to a 3D coordinate system of the Kinect depth camera to obtain the 3D coordinator of the optimal pupil center point, ;using the 3D eyeball model to estimate the horizontal sight line angle, ;and designing a test system and obtaining the sight line angle through calibration. The method is simple in operation, good in instantaneity, and low in cost and has low limitation on the head of the tester and has the high accuracy.

The invention provides a method and kit for quantitatively detecting the methylation degree of a DNA specific site. The method provided by the invention comprises the following steps: 1) designing a PCR primer in accordance with a DNA sequence to be detected, and using the PCR primer for amplifying the DNA to be detected; 2) designing a pair of TaqMan probes in accordance with a CpG site-enriched region in the PCR product obtained by amplification in the step 1), wherein one probe is a CG probe designed in accordance with the DNA of which the CpG site is completely methylated, and the other probe is a TG probe designed in accordance with the DNA of which the CpG site is completely non-methylated and is used for specifically combining the PCR product and detecting the PCR product; and 3) carrying out real-time quantitative PCR based on the primer designed in the step 1) and the probes designed in the step 2), thus detecting the methylation degree of the DNA to be detected. By using the method, a specific methylated site can be detected, and the methylation states of other sites can not affect the detection result. The kit provided by the invention can be used for detecting the methylation degree of a DNA specific site.

The present invention relates to a sample analyzer, which comprises a sample suction device, a reaction cell, a detector, a cleaning liquid storage tank, a liquid transmission device and a cleaning controller, wherein the sample suction device sucks and conveys a sample to be tested to the reaction cell so as to be subjected to a measurement reaction, the detector detects the detection items of the reactant generated by the measurement reaction, a cleaning solution is stored in the cleaning liquid storage tank, and the cleaning controller is separately connected to the sample suction device and the detector so as to obtain detection parameters, determines whether the residual reactant exceeds a standard according the detection parameters, and controls the liquid transmission device to suckthe cleaning solution from the cleaning liquid storage tank so as to perform cleaning operation when the residual reactant exceeds the standard. The invention further relates to a cleaning control method of the sample analyzer.

The invention relates to an apparatus for detecting mechanoluminescent performance of a luminescent material. The apparatus comprises a dark chamber, an objective table, an ultraviolet lamp, a pressure signal monitoring system, and an optical signal monitoring system; the dark chamber comprises an internal layer dark chamber and an external layer dark chamber, the internal layer dark chamber is placed at one side of the bottom of the external layer dark chamber, and the ultraviolet lamp is arranged in the external layer dark chamber and positioned over the internal layer dark chamber; the pressure transducer is arranged in the external layer dark chamber, and opposites a force application port over the external layer dark chamber, a pressure signal output port is arranged at an outer wallof the external layer dark chamber, and the pressure transducer, a pressure transmitter and the pressure signal output port are electrically connected in order; an objective table is arranged over theinternal layer dark chamber, a sample installation groove is arranged at the objective table, the sample installation groove and the force application port over the external layer dark chamber, and the pressure transducer are coaxially arranged. The apparatus has the advantages that simultaneous and synchronous monitoring is carried out, a pressure signal of mechanical excitation force and the generated optical signal are recorded, and the monitoring result is accurate. The apparatus belongs to the technical field of detection.

The invention discloses a non-phase-locked-loop rotating vector detection method suitable for power grid voltage distortion and imbalance. The method is completed according to the following steps that power grid voltage and load current instantaneous values under the distortion and imbalance state are acquired via voltage Hall components and parts and current Hall components and parts respectively; the power grid voltage and load current instantaneous values processed by a conditioning circuit are converted into digital signals by an A / D module and then inputted to a DSP processor; the digital values of the power grid voltage and load current instantaneous values are processed by the DSP processor so that a specific time of harmonic component is obtained via detection; and reactive and active components are obtained via detection by the DSP processor according to the specific time of harmonic component obtained via detection. According to the non-phase-locked-loop rotating vector detection method suitable for power grid voltage distortion and imbalance, problems in the prior art that the detection process is complex, detection results are interfered by voltage frequency deviation and the specific time of harmonic current cannot be accurately detected are solved.

The invention relates to a method for detecting microorganisms of cosmetics. The method comprises the following steps: firstly, respectively preparing a high-concentration cosmetic sample to-be-detected solution, a low-concentration cosmetic sample to-be-detected solution, a sample-free blank control solution and a microorganism-containing positive control solution; then respectively injecting the prepared high-concentration cosmetic sample to-be-detected solution, the prepared low-concentration cosmetic sample to-be-detected solution, the prepared sample-free blank control solution and the prepared microorganism-containing positive control solution in four culture dishes containing culture mediums and carrying out colony counting, clearly observing a bacterium culture result, and identifying bacteria through comparison. According to the method, a sample is diluted by adopting an SCDLP (Soya Casein Digest Lecithin Polysorbate) enriched liquid, thus the detection sensitivity can be remarkably improved; and a lecithin Tween 80 nutrient agar medium is used as a culture medium of escherichia coli, staphylococcus aureus and pseudomonas aeruginosa, thus the method is simple to operate, and accurate in detection result.

The invention relates to a Yolov3 network computing acceleration system based on an FPGA and an acceleration method thereof. The system comprises an ARM and FPGA platform architecture, an off-chip storage area, an AXI_M interface and an AXI_S interface, the ARM platform architecture comprises a core processor and a data and memory controller, and the FPGA platform architecture comprises an acceleration core unit, an input cache end and an output cache end; and the core processor comprises an ARM Cortex-A53 CPU and an L2 cache region, the off-chip storage region comprises an SD card and an external DDR4, and the acceleration core unit comprises a data matrix vector array and a calculation module. The input cache end and the output cache end adopt a multi-channel parallel reading and writing-back mode to replace a traditional single-channel reading and writing mode, and the bandwidth of the Zynq chip is utilized to the maximum extent. Double cache regions and a register array are designed at the input cache end, efficient data multiplexing is achieved, and the bandwidth is multiplied.

The invention discloses a method for measuring the circulation area of complex profile hole, main contents of which are that the air pressure P1* in front of the workpiece profile hole to be measured is adjusted to the supercritical pressure ratio, namely not less than 2 times of atmospheric pressure Po in order to ensure that air passes through the profile hole at a critical speed, and the air after further expansion is discharged into the atmosphere through an exhaust pipe. Measuring the air pressure P1* in front of the profile hole, the air temperature T1* and the air flow Ga through a pipeline, calculating the coefficient m of the air through the air adiabatic index K and the gas constant R, and substituting these data into the flow equation of compressible flow to calculate the circulation area of the complex profile hole to be measured. Compared with the prior art method of measuring the area of the profile hole with the air power, the method disclosed by the invention, which measures the circulation area of the complex profile hole with the supercritical pressure ratio, has the advantages of the high measurement precision, the large measurement range, the strong commonality of a measuring device and the convenient operation, and solves the problem of precise measurement of the circulation area of the complex profile hole with the small circulation area.

The invention discloses an avian pneumovirus Taqman probe fluorescent quantitative RT-PCR detection kit and an application thereof. The kit contains a composition for detecting the avian pneumovirus, and the composition is composed of a primer pair and a probe; the primer pair is composed of a primer 1 and a primer 2, the primer is single-stranded DNA represented by sequence 1 in a sequence table, and the primer 2 is single-stranded DNA represented by sequence 2 in the sequence table; and the sequence of the probe is sequence 3 in the sequence table. Experiments prove that the kit has the advantages of short detection time, strong specificity, high sensitivity, simple operation and low price when the kit is used for the avian pneumovirus Taqman probe fluorescent quantitative RT-PCR detection.

The invention discloses a detection kit and a preparation method thereof for drugs of benzodiazepines. The detection kit provided by the invention includes a reagent strip, wherein the reagent strip includes sample filtering paper, immune colloid gold paper, an immune cellulose nitrate membrane and water absorbent paper which are arranged sequentially from the sample feeding end, wherein the immune colloid gold paper is a fiber glass gasket coated by an anti-benzodiazepine monoclonal antibody labeled by colloid gold; the immune cellulose nitrate membrane is a cellulose nitrate membrane provided with a detection line and a quality control line; the detection line is coated with a benzodiazepine-bovine serum albumin compound; and the quality control line is coated with goat-anti-mouse IgG. The invention further provides a preparation method of the detection kit for drugs of benzodiazepines. Through adopting the detection kit and the preparation method provided by the invention, the consumption of monoclonal antibodies can be reduced, and the manufacturing cost is saved.

The invention provides a novel leak test valve which comprises a valve body and a rotatable valve core, wherein, the valve core is arranged in the valve body; the valve body is equipped with threaded holes, exhaust vents and locating threaded holes; the first threaded hole is connected with an air compressor, the second threaded hole and the third threaded hole are respectively connected with a standard container and a container to be tested, and the fourth threaded hole and the fifth threaded hole are respectively connected with two ends of a pressure sensor; the valve body is equipped with inner circular grooves at the second threaded hole and the third threaded hole; the valve core is equipped with a pressurized shift, a pressure measurement shift, a balance shift and an exhaust shift; the circumference of the valve core is equipped with tanks along the corresponding axial positions of the shifts; a pressurized tank can enable the inner ends of the first threaded hole, the second threaded hole and the third threaded hole to be communicated together; two pressure measurement tanks can enable the inner ends of the fourth threaded hole and the fifth threaded hole to be communicated with the inner circular grooves at the corresponding sides of the threaded holes respectively; a balance tank can enable the inner ends of the fourth threaded hole and the fifth threaded hole to be communicated together; and two exhaust tanks are matched with two radial holes in a valve core and can enable the fourth threaded hole and the fifth threaded hole to be communicated with the corresponding exhaust vents respectively.

The invention relates to a device and a method for measuring the sizes of running vehicles. A top laser radar which is used for emitting laser waves toward the direction of coming vehicles to scan the fronts of the bodies of vehicles to be measured is mounted over a vehicle-running lane, and the front end of the advancing direction of vehicles, a side laser radar and a laser rangefinder are respectively mounted within the plane of the entrance of a detection area perpendicular to the lane and at both sides of vehicles to be measured, the side laser radar is positioned above one side of vehicles to be measured, the laser rangefinder is positioned below the other side of vehicles to be measured, and moreover, the distance between the side laser radar and the laser rangefinder is a set distance. The invention can measure the sizes of vehicles as vehicles run, and has no special requirement on the running speed of vehicles, the final detection result is not affected even if vehicles run at non-uniform speeds and even stop for a short time, the detection efficiency is increased, the invention is highly applicable, and thereby the defect of other methods is overcome in terms of applicability; the cost is relatively low; and the precision is greatly increased in comparison with the precision of manual measurement.

The invention discloses a method for preparing a solid granular biochemical reagent, solving the problem that the conventional biochemical reagent cannot be accurately metered under the condition of zero gravity or bumping. The method mainly comprises the following steps of: (1) preparing the biochemical reagent; (2) distributing liquid drops through a liquid dispensing pump; (3) dropping the liquid drops into liquid nitrogen so that the liquid nitrogen in contact with the liquid drops absorbs heat so as to be gasified to form a gas flow, wherein the gas flow enables the liquid drops to be floated on the surface of the liquid nitrogen; and when the liquid drops are completely solidified, the liquid drops are formed into ice pellets and sunk to the bottom of the liquid nitrogen; and (4) freeze-drying the ice pellets in a freeze dryer, thereby obtaining the finished product. The method is high in accuracy, simple and convenient for operation, good in detection result and the like.

The invention discloses a whole blood sample pretreatment device. The pretreatment device comprises a pretreatment pipe and a pretreatment diluent. The pretreatment diluent is accommodated in the pretreatment pipe. A plurality of stainless steel beads are arranged in the pipe body. The stainless steel beads are added to accelerate the uniform mixing speed of liquid in the pretreatment pipe. The pretreatment diluent comprises a buffer solution, a surfactant, inorganic salt, a stabilizer and a preservative. The pretreatment pipe comprises a pipe body and a pipe cap. The pipe body is a cylindrical tube, the bottom of the pipe body is provided with an arc-shaped concave surface, the pipe cap is arranged at a top opening of the pipe body, and the pipe cap is in sealed connection with the pipe body. The whole blood sample pretreatment device disclosed by the invention can be used for pretreating a collected whole blood sample without separating serum or plasma, the pretreated whole blood sample can be widely applied to detection of a full-automatic biochemical analyzer and a specific protein instrument, the detection efficiency is higher, and the stability is good. The stabilizer component in the pretreatment diluent is beneficial to the preservation of the to-be-detected substance in the sample, so that the detection accuracy is improved, and the repeatability is good.

The invention discloses a colloidal gold test paper strip for semiquantitatively detecting the concentration of a GFAP (Glial Fibrillary Acidic Protein) in a human serum. The colloidal gold test paper strip is produced by adhering a detection diaphragm, a colloidal gold combined diaphragm and a water-absorbing paper layer to a PVC (Poly Vinyl Chloride) board, wherein the colloidal gold combined diaphragm is coated by a colloidal gold-labeled antibody for resisting the GFAP; a detection line and a quality control line are arranged on the detection diaphragm; a capture antibody for resisting the GFAP is fixedly arranged on the detection line; a rabbit anti-mouse IgG (Immunoglobulin G) is fixedly arranged on the quality control line; and a sampling part, the colloidal gold combined diaphragm, the detection line, the quality control line and the water-absorbing paper are sequentially arranged on the test paper strip from the bottom up. The test paper strip disclosed by the invention can be used for semiquantitatively detecting the GFAP, has the advantages of high specificity, high sensitiveness, high accuracy, quickness, simpleness and convenience in operation, low expense, no need ofany apparatus, time-and-labor saving and the like as well as has favorable application prospect.

The invention discloses an unmanned vehicle based on an AI technology, an AR virtual city system, an AI control system, an unmanned vehicle practical training platform and an unmanned vehicle practical training method. The unmanned vehicle practical training platform comprises the unmanned vehicle, the AR virtual city system and the AI control system, wherein the AR virtual city system constructsa virtual city environment for unmanned vehicle practical training; the unmanned trolley runs in the virtual city environment and senses a current virtual scene to obtain scene sensing data; the AI control system acquires virtual city environment information and scene sensing data in real time, and generates a control instruction for controlling the unmanned trolley to run in combination with a preset control rule; and the unmanned trolley runs in the virtual city environment according to the control instruction of the AI control system. The unmanned vehicle practical training platform can simulate the required embedded, wireless communication and virtual scene, is controlled by adopting an AI artificial intelligence algorithm, and truly opens the development technology environment required by each level of the unmanned driving technology through adopting the virtuality and reality combination technologies.

The invention discloses a method for the surface peeling of powder superalloy. An oxalic acid solution is taken as electrolyte, and by means of controlling corrosion voltage, corrosion current and corrosion time, the surface of the powder superalloy is subjected to corrosive peeling layer by layer until the residual stress of a sample is 0. The method disclosed by the invention is suitable for powder superalloy samples of different shapes and sizes, in addition, additional deformation cannot be caused by carrying out peeling removal on all surfaces of each sample, a surface deformation disturbance layer is easy to remove, and the method is simple to operate and carry out, obvious in removal effect and free from influencing a test result.

The invention discloses a nano-material-based detection method for heroin in biological samples. The method comprises the following steps: separately preparing a nano-gold composite and a magnetic nanocomposite; mixing the nano-gold composite and the magnetic nanocomposite with a biological sample; and detecting the intensity changes of the ultraviolet-visible absorption spectrum of mixed liquor so as to realize qualitative and quantitative analysis of existence or content of heroin in the biological sample. The nano-material-based detection method is high in reaction efficiency, good in selectivity and environmental adaptability and high in stability and can reach low-and-medium limit detection levels prescribed in the national standards in China.

The invention discloses a novel environment monitoring device, which comprises a control box body, the interior of the control box body is provided with a partition, and the inner bottom surface of the control box body is provided with a storage battery, a processor, a storage device, a WIFI connector and a / D converter, the storage battery, processor, storage, WIFI connector and A / D converter are located under the partition, and the center of the upper surface of the partition is equipped with an electric telescopic rod, which can drive the two The upper end of an L-shaped connecting frame is opened, and drives the connecting seat to move upwards, so that the temperature sensor, dust sensor, humidity sensor and decibel meter extend out of the control box. In rainy days, water can be prevented from entering the control box, effectively The electrical components are protected. The new environmental monitoring device has a simple structure and is easy to operate. It can not only protect the electrical components, but also make the service life of the device longer, and does not affect the detection results of the electrical components.

The invention discloses cleaning fluid which comprises an anionic surfactant, a non-ionic surfactant, an alkali metal hydroxide, plantrennet and a buffer agent for stabilizing the pH value to be greater than 12.0. When being used, the cleaning fluid disclosed by the invention has the characteristics of low protein substance residue rate, low fat substance residue rate, good in-batch repeatability of clinical projects, low cross contamination, low reactant deposition and the like, and meanwhile the testing result of a biochemical analyzer is not affected; a liquid path system and a reaction cup of the biochemical analyzer are not corroded in the washing process; meanwhile, all components of the cleaning fluid disclosed by the invention can be biologically degraded, no potential pollution to the environment is caused, and the requirements of environmental protection are met.

The invention provides a rapid detection method for starting and shunt running of an intelligent electric energy meter. The method comprises the following steps: 1, a preset starting current Ix and apreset time threshold value A are preset; 2, according to nominal parameters of the electric energy meter, calculating a theoretical starting time limit tQ for starting test detection of the electricenergy meter and a theoretical shunt running time limit delta t for shunt running test detection; 3, after the starting test detection or the shunt running test detection is started, the preset starting current Ix is applied in the front A*tQ or A*delta t of the theoretical starting time limit tQ or the theoretical shunt running time limit delta t of the shunt running test detection, and a theoretical starting current IQ is applied in the back (1-A)*Tx or (1-A)*delta t of the theoretical starting time limit tQ or the theoretical shunt running time limit delta t of the shunt running test detection; and 4, after the theoretical starting time limit tQ or the theoretical shunt running time limit delta t is ended, according to the change of an actual pulse of the electric energy meter, and in combination with starting test detection standards or shunt running test detection standards, a detection result of a starting test or a shunt running test is judged.

The invention discloses a simplified library construction method and a reagent. The construction method and the reagent, disclosed by the invention, can finish fragmentation and final segment repairing in one step; a product obtained by the fragmentation and the final segment repairing can be not purified and a library enrichment step is omitted in a whole library construction method; a sequencinglibrary, which is finally obtained, can meet sequencing online requirements, and DNA (Deoxyribonucleic Acid) loss caused by multi-step purification is also reduced; a detection result of a sample isnot influenced.

The invention discloses a method for screening high-output stable cell strain by HTRF, comprising the following steps of: (1) in a microwell plate, culturing cell strain monoclone for expressing an object antibody or a fusion protein; (2) absorbing cultured cell supernatant, and added the cell supernatant into the microwell plate for screening, adding Human-IGg-XL665 and Anti-Human-IGg-Fc-Cryptate into micropores, contrasting by using the object antibody or the fusion protein to be detected as a standard substance, reacting under room temperature for 2.5h or overnight, detecting an absorbance ratio of A665nm to A615nm based on a HTRF method principle, and judging expression output of the cell strain clone based on the ratio. The method has advantages of short time consumption, high flux, simple operation and stable result, can effectively screen high-expressed cell strain clone in a short time, and accelerates a process in a bio-pharmaceutical industry.

The invention relates to a traditional Chinese manipulation detection method. A determination system comprises a cardiovascular function detection instrument, a muscular tension sensor, a surface electromechanical pickup, a contact type temperature measuring instrument and a flexible three-dimensional mechanical quantity determination pad which are arranged in the same cabinet body, wherein a probe of the flexible three-dimensional mechanical quantity determination pad is introduced out of the cabinet body, each signal output wire is connected onto the same computer, the determination pad is flexible pressure-sensitive conductive rubber filled with two electrode layers, the electrode layers consist of conducting wires and electrodes, and the two layers of electrodes are in staggered distribution. The conducting wires are formed by winding insulation elastic wires and fine conductive metal wires spirally wound on the surfaces of the insulation elastic wires, the first layer of conducting wires and the second layer of conducting wires are in a space crossing mode, and an included angle is 70 degrees to 80 degrees; and the flexible electrodes are electrically connected with the fine conducting metal wires. The determination system can obtain the clinic data including corresponding physiological parameters of manipulation receivers, the intensity, the position, the frequency and the acting time of single-point or multi-point or certain-area three-dimensional manipulation force exerted by manipulation doctors and other data in clinics in real time, the later-stage analysis study can be carried out, and the curative effect of the manipulation is evaluated.