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Method for detecting microorganisms of cosmetics

A detection method and technology of cosmetics, applied in the direction of microorganism-based method, measurement/inspection of microorganisms, microorganisms, etc., can solve the problem of low detection rate of pathogenic bacteria, complicated detection methods of microorganisms, failure to detect microorganisms, etc. problem, to achieve the effect of simple detection process and improved detection sensitivity

Inactive Publication Date: 2014-03-05
中山鼎晟生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies in recent years have shown that in the process of microbial detection of cosmetics, the detection rate of important pathogenic bacteria such as Staphylococcus aureus and Escherichia coli is low. There are two main reasons for this result: First, some manufacturers are in In the process of producing cosmetics, the quality is strictly controlled, and the cosmetics are not polluted by microorganisms; secondly, the defects of other ingredients in cosmetics or existing detection technology prevent microorganisms from being detected
The method for microbial detection of cosmetics proposed in the 2007 edition of "Hygienic Standards for Cosmetics" is complicated and has technical defects. , an easy-to-operate detection method is essential

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (2) Inject the high-concentration cosmetic sample pre-test solution, low-concentration cosmetic sample pre-test solution, blank control solution and positive control solution prepared in step (1) into four petri dishes containing medium for cultivation And count the colonies, carefully observe the bacterial culture results, and identify the bacteria by comparison.

[0033] (1) Aseptically weigh 10g of hydrophilic sample and add it to a glass bottle of 100ml SCDLP enrichment solution, mix well to prepare a 1g / ml high-concentration cosmetic sample preparation solution; aseptically weigh 1g of hydrophilic Add the sample into a glass bottle of 100ml SCDLP enrichment solution, and mix thoroughly to prepare a low-concentration 0.1g / ml cosmetic sample preparation solution.

[0034] (2) Add 100ml of SCDLP enrichment solution into a glass bottle to prepare a blank control solution.

[0035] (3) Add 1g of standard Staphylococcus aureus NICPBP26112, Escherichia coli standard stra...

Embodiment 2

[0039] (1) Aseptically weigh 20g of the hydrophilic sample and add it to a glass bottle of 100ml SCDLP enrichment solution, mix well to prepare a 2g / ml high-concentration cosmetic sample preparation solution; aseptically weigh 2g of the hydrophilic sample Add the sample into a glass bottle of 100ml SCDLP enrichment solution and mix thoroughly to prepare a low concentration 0.2g / ml cosmetic sample test solution.

[0040] (2) Add 100ml of SCDLP enrichment solution into a glass bottle to prepare a blank control solution.

[0041] (3) Add 1g of standard Staphylococcus aureus NICPBP26112, Escherichia coli standard strain AMMS8099, Pseudomonas aeruginosa strain NICPBP10211, Candida albicans strain ATCC10231 and yeast standard strain into a glass bottle of 100ml SCDLP enrichment solution, Mix well to prepare positive control solution.

[0042](4) Take 2g / ml high-concentration cosmetic sample pre-test solution, 0.2g / ml low-concentration cosmetic sample pre-test solution, positive con...

Embodiment 3

[0045] (1) Aseptically weigh 10g of hydrophobic samples into a glass bottle of 100ml of SCDLP enrichment solution, add 5ml of Tween 80, and mix well to prepare a 1g / ml high-concentration cosmetic sample preparation solution; sterile weigh Take 1g of the hydrophilic sample and add it to a glass bottle of 100ml SCDLP enrichment solution, and mix well to prepare a 0.1g / ml low-concentration cosmetic sample preparation solution.

[0046] (2) Add 100ml of SCDLP enrichment solution into a glass bottle to prepare a blank control solution.

[0047] (3) Add 1g of standard Staphylococcus aureus NICPBP26112, Escherichia coli standard strain AMMS8099, Pseudomonas aeruginosa strain NICPBP10211, Candida albicans strain ATCC10231 and yeast standard strain into a glass bottle of 100ml SCDLP enrichment solution, Mix well to prepare positive control solution.

[0048] (4) Take 1g / ml high-concentration cosmetic sample preparation solution, 0.1g / ml low-concentration cosmetic sample preparation so...

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Abstract

The invention relates to a method for detecting microorganisms of cosmetics. The method comprises the following steps: firstly, respectively preparing a high-concentration cosmetic sample to-be-detected solution, a low-concentration cosmetic sample to-be-detected solution, a sample-free blank control solution and a microorganism-containing positive control solution; then respectively injecting the prepared high-concentration cosmetic sample to-be-detected solution, the prepared low-concentration cosmetic sample to-be-detected solution, the prepared sample-free blank control solution and the prepared microorganism-containing positive control solution in four culture dishes containing culture mediums and carrying out colony counting, clearly observing a bacterium culture result, and identifying bacteria through comparison. According to the method, a sample is diluted by adopting an SCDLP (Soya Casein Digest Lecithin Polysorbate) enriched liquid, thus the detection sensitivity can be remarkably improved; and a lecithin Tween 80 nutrient agar medium is used as a culture medium of escherichia coli, staphylococcus aureus and pseudomonas aeruginosa, thus the method is simple to operate, and accurate in detection result.

Description

technical field [0001] The invention belongs to the field of cosmetic safety testing, and in particular relates to a testing method for cosmetic microorganisms. Background technique [0002] With the development of my country's economy and society and the improvement of people's quality of life, the cosmetics industry has developed rapidly in recent years, and the types and quantities of cosmetics have also increased day by day. The quality of cosmetics has attracted the attention and attention of relevant regulatory authorities and many consumers. In order to improve the skin care and makeup effects of cosmetics, manufacturers often add a variety of proteins, fats, animal tissues, vitamins, inorganic salts and water to them. The pH value of cosmetics is 4-7. Therefore, cosmetic ingredients are rich in nutrients. The ingredients and the appropriate pH value provide good conditions for the growth and reproduction of microorganisms, which can easily lead to spoilage of cosmeti...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12Q1/06C12Q1/14C12R1/385C12R1/445C12R1/19C12R1/725
Inventor 郭狄
Owner 中山鼎晟生物科技有限公司
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