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Enzyme biofuel cell (EBFC)-based self-powered bacteria biological sensor and application thereof

A biosensor and self-powered technology, applied in the field of biosensing, can solve the problems of difficult, expensive, and complex detection, and achieve the effects of improving detection sensitivity, low cost, and high selectivity

Inactive Publication Date: 2019-01-22
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many methods for detecting pathogenic bacteria in food, including polymerase chain reaction (PCR), biochemical immunoassay, next-generation sequencing (NGS), DNA probe, surface-enhanced Raman scattering (SERS) spectroscopy, but It is expensive and complicated, and it is difficult to detect

Method used

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  • Enzyme biofuel cell (EBFC)-based self-powered bacteria biological sensor and application thereof
  • Enzyme biofuel cell (EBFC)-based self-powered bacteria biological sensor and application thereof

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Embodiment 1

[0038] EBFC-based self-powered bacterial biosensor for detection of V. parahaemolyticus.

[0039] (1) Preparation of AuNPs:

[0040] 4 mL of HAuCl 4 Add to 96mL of secondary water, stir and heat to boiling, quickly add 10mL, 38.8mM sodium citrate solution and boil for 15min, then cool to obtain wine red transparent liquid is AuNPs;

[0041] (2) Preparation of CNT / AuNPs:

[0042] Weigh 8mg of carbon nanotubes (CNT) and dissolve them in 4mL, 1% polydiallyldimethylammonium chloride (PDDA) for ultrasonic treatment for 30min, then centrifuge and wash the resulting solution at 15000rpm for 15min for several times, and take the supernatant The obtained black solid was fixed to volume with 1 mL of secondary water, and the solution obtained in step A was added to 10 mL for adsorption overnight, then ultrasonically washed at 5000 rpm for 6 min, and the obtained precipitate was dispersed in 1 mL of secondary water to obtain a CNT / AuNPs mixture;

[0043] (3) Preparation of MCH / aptamer / ...

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Abstract

The invention builds an enzyme biofuel cell (EBFC)-based self-powered bacteria biological sensor and application thereof, and belongs to the technical field of biological sensors. Electrons from a biological positive electrode can be received by prussian blue (PB) and can be converted to prussian white (PW), blue of the PB also can be converted to transparent PW by obtaining the electrons, the changing speed from blue to nature is dependent on concentration of vibrio parahaemolyticus, and the color can be restored to an original PB state by connecting the color to the biological positive electrode. When target vibrio parahaemolyticus exists, an open-circuit voltage generated by the EBFCs is reduced accordingly, the amplitude of the open-circuit voltage is dependent on the concentration ofthe vibrio parahaemolyticus identified by a target adaptor. Due to a steric-hinerance effect of the vibrio parahaemolyticus, fuel glucose is prevented from being approaching an active site of glucosedehydrogenase on the positive electrode, and the color of the prussian blue is reduced. By the EBFC-based self-powered bacterial biological sensor, simple, convenient, rapid, sensitive and high-efficiency detection of food pathogens is achieved.

Description

technical field [0001] The present invention constructs a self-powered biosensing platform based on visualized and reproducible enzyme biofuel cells (EBFCs) by integrating Prussian blue (PB) electrochromism and specific binding of aptamers to antigens for The invention relates to detection of vibrio parahaemolyticus, which belongs to the technical field of biosensing. Background technique [0002] Enzyme biofuel cells (EBFCs) are a new type of green energy conversion technology with promising application prospects due to their mild operating conditions, which can extract bioenergy from biochemical reactions. The self-powered biosensor based on EBFC is a kind of sensor that uses the battery performance output as the analysis detection signal, and the sensor signal is proportional to the concentration of the detected analyte. Its specific advantages are mainly manifested in: (1) The equipment is simple. The detection process only needs two electrodes, that is, the negative a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327
CPCG01N27/26G01N27/3272
Inventor 李峰盖盼盼于汶谷成成侯婷
Owner QINGDAO AGRI UNIV
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