Integrated minitype magnetic pump type capillary electrophoresis chip

Abstract

On surface of substrate B, following structure are prepared: buffer solution groove, spent solution groove of electrophoretic separation pipe, sample introduction pipe and spent solution groove, flow channel of minitype magnetic pump and sample introduction groove. Electrodes are spluttered on two walls of whole flow channel. Sample introduction groove, buffer solution groove, two spent solution grooves and tapping point of electrode on substrate B are aligned to and bonded to relevant exits of substrate A. permanent magnet is installed below electrode of substrate B. minitype magnetic pump provides and changes multiple samples for continuous electrophoresis test. When a sample test is finished, former sample is discharged to spent solution groove, and new sample is delivered to separation point. The invention shortens time of continuous test and is available in repeatable use.

Description

Technical field [0001] The invention relates to a biochemical detection device, in particular to an integrated miniature electromagnetic pump type capillary electrophoresis chip. Background technique [0002] Capillary Electrophoresis (CE for short) is a new type of separation and analysis technology combining electrophoresis and chromatography developed rapidly in the early 1980s. It has the characteristics of high analysis efficiency, fast analysis speed, less sample consumption and wide application range. , has become an important means of separation and analysis in the 1990s, and has a good application prospect in the fields of biology, chemistry, medicine, environmental protection, and food. The capillary electrophoresis chip technology developed rapidly after the 1990s is based on micro-processing technology, with crystalline silicon, glass, plastic (mainly plexiglass), ceramics and silicone rubber as the base material, and the sample is processed by capillary electrop...

AI Technical Summary

Problems solved by technology

The use of manual sample change has an impact on the speed and detection accuracy of continuous detection of multiple samples, especially in the problem of taking too long;

[0004] (2) The capillary electrophoresis chip uses electroosmotic sample injection, high working voltage and poor stability. At the same time, electroosmotic flow is inevitably accompanied by electrophoretic separation. This separation process is irreversible and affects the quality of the input sample.

[0005] Therefore, the use of electroosmotic sample injection has a negative impact on the accuracy of sample detection;

[0006] (3) At present, most capillary electrophoresis chips are disposable, there is a relatively large waste, and the detection cost is high

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