33results about How to "Shorten lysis time" patented technology

The invention provides a tire rubber pyrolysis method combining pyrolysis gas circulation heating and microwaves. The method includes the steps of A, preprocessing tire rubber; B, feeding the preprocessed tire rubber into a composite pyrolysis cavity; C, using a gas heating device to heat gas media to 350-700 DEG C; D, continuously feeding the heated gas into the composite pyrolysis cavity, and allowing the heated gas and the microwaves to jointly act on the tire rubber so as to perform pyrolysis to obtain solid products and mixed gas products; E, performing oil-gas separation on the mixed gas products to remove pyrolysis oil; F, partially feeding the gas obtained after the oil-gas separation in the step E into the gas heating device of the step C, and heating the gas to 350-700 DEG C for the circulation pyrolysis of the step D. By the tire rubber pyrolysis method, pyrolysis energy consumption and pyrolysis time can be reduced and cost is saved.

The invention relates to a rapid quantitative detection method for drugs in hair. The rapid quantitative detection method comprises the following steps that (1) hair extraction is conducted, specifically, the drugs in the hair are fully released through mechanical grinding and bioenzyme extraction; (2) a sample is prepared from a to-be-detected object treated in the step (1), and the sample is dropwise added onto a reagent strip of a hair drug fluorescence immunochromatographic kit; and (3) the hair drug fluorescence immunochromatographic kit is detected through a dry immunofluorescence analyzer, and the content of an analyzed object in the to-be-detected sample is calculated. According to the rapid quantitative detection method, the advantages that sampling for hair detecting is easy, preservation is easy, the detecting time limit is long (from several weeks to several months), and information is comprehensive are reserved; the defects that conventional hair detection is complex in pretreatment, cumbersome operation, long in result analysis time and the like are overcome; the detecting time of the drugs in the hair is shortened from several hours and several days to fifteen to twenty minutes, the operation steps and technical requirements are greatly simplified, and thus non-professional technical personnel can also operate and use the rapid quantitative detection method.

The invention provides a solid organic material cracking method and system. The method comprises the following steps of: A, pre-treating a solid organic material; B, sending the pre-treated solid organic material into a cracking device; C, heating a gas medium to 350-700 DEG C; D, continuously importing the heated gas medium into the cracking device to act on the solid organic material with microwave, so as to carry out cracking to obtain a solid product and a mixed gaseous product; and E, importing a part of the obtained mixed gaseous product into a gas heating device in the step C so as to heat the mixed gaseous product to 350-700 DEG C to be circularly used for the cracking in the step D. The process can effectively decrease the cracking energy consumption and the cracking time, and can save the cost.

The invention relates to the technical field of biology, in particular to a kit capable of being applied to high-throughput extraction of genome DNA from an animal tissue and an extraction method thereof. The kit capable of being applied to the high-throughput extraction of the genome DNA from the animal tissue comprises lysate buffer, binding buffer, a washing solution I, a washing solution II, eluant, protease K and magnetic beads, the composite effect of reagents is utilized to fully digest, split, damage cells for removing proteins to obtain high-purity DNA. Compared with a kit sold in themarket, the kit capable of being applied to the high-throughput extraction of the genome DNA from the animal tissue has the advantages that the pyrolyzing rate is quick, the extraction purity is high, the extraction volume is large, the used reagents are safe, stable and efficient, the kit is especially suitable for high-throughput automated production, so that the extraction efficiency of the genome DNA is greatly improved, the error is reduced, and the extraction is more accurate.

The invention discloses a method of performing electrochemical strengthening liquid phase catalysis and low-temperature splitting decomposition on biomass. According to the method, ionic liquid is taken as a catalytic reaction medium in a biomass splitting decomposition process, so that the biomass is subjected to electrochemical stimulation in the ionic liquid to make a splitting decomposition reaction and generate a high-added-value split product. An electricity-assisted splitting decomposition reactor used in the method comprises a barrel, a screw type anode and a grid cylinder type cathode, wherein the screw type anode is arranged in the grid cylinder type cathode and is driven to rotate by a motor arranged on the barrel, the screw type anode and the grid cylinder type cathode are arranged in the barrel, the upper end of the barrel is provided with a feeding hole and an air outlet, and the lower part of the barrel is provided with a discharge hole, an ionic liquid inlet and an air inlet. The biomass is heated evenly, the chemical bond of lignocellulose is enabled to break, and an ionic pair midbody is generated and is subjected to electrical field stimulation, thus increasing the splitting decomposition rate; the efficiency is high, the process is easy to control, and the use ratio of the biomass is improved.

Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and / or improving the kinetics of luciferase-catalysed reactions.

The invention discloses a cracking apparatus for producing myrcene by cracking Beta-pinene; the cracking apparatus comprises cracking apparatus housing (2), a plurality of groups of cracking pipe (6), intake pipe (1), exhaust pipe (8) and jetting device (3); one end of the cracking apparatus housing (2) is internally provided with the jetting device (3), the intake pipe (1) is connected to the jetting device (3), the other end of the cracking apparatus housing (2) is externally provided with the exhaust pipe (8), and each group of cracking pipes (6) includes a plurality of cracking pipes mutually concentric; the surface of each cracking pipe (6) is provided with a catalyst layer. The cracking apparatus can provide increased specific surface area for the cracking pipes, facilitates full contact of Beta-pinene gas with the catalyst on the surface of the cracking pipes to allow cracking, and accordingly provides increased yield and purity of finished myrcene; the cracking apparatus also has the advantages of low energy consumption, low cost, high production efficiency, simple structure and the like, and facilitates industrial production.

The invention provides a cracking technology of a solid organic material. The technology comprises the following steps: heating a gas medium to 200-800 DEG C, continuously introducing the heated gas medium to a cracking device, and cracking the solid organic material under the common action of the heated gaseous medium and microwaves, wherein the gas medium is anyone or a combination comprising above one of nitrogen, water vapor, carbon dioxide and inert gas. The technology aims at the particularity of the solid organic material, innovatively combines treatment of gas obtained after cracking of the solid organic material with microwave cracking, and improves the controllability of the cracking treatment process through adopting specific technologic steps and parameters to substantively reduce the energy consumption and substantively improve the cracking efficiency.

The invention discloses a method for accurately extracting DNA from wood targeted cells; through removal of exogenous pollution on a wood surface, in-situ detection of wood DNA, radial section of woodtissues, precise separation of the wood targeted cells enriched in DNA under an inverted microscope, cracking of wood cell walls, removal of a wood extract, DNA precipitation and adsorption, dissolution, purification, PCR amplification and other steps, DNA is extracted accurately from the wood tissues. The method aims at the wood targeted cells to carry out DNA accurate extraction, the quantity and purity of DNA extracted from the wood tissues are improved, and the interference of other types of cells of wood on DNA extraction is reduced. The method can be directly applied to study of molecular biology and provide a new technical support for identification of wood species.

The invention discloses a bacterium nucleic acid extraction solution obtained by a magnetic bead method. The bacterium nucleic acid extraction solution comprises the following components of a rare earth compound, an acid buffer solution, a surfactant and a chelating agent. Through cooperative usage of the rare earth compound and the surfactant, the bacterium nucleic acid extraction efficiency is improved, and extraction time is shorter.

The invention relates to a tobacco developing and anti-faking pelleted seed. The weight ratio of seeds, a seed coating agent and a bonding agent is 1 to 50 to 10; each tobacco developing and anti-faking pelleted seed comprises three pelleting layers, an inner layer is a nucleation granulation layer coating the seed, a middle layer is a primary pelleting layer coating the nucleation granulation layer, an outer layer is a secondary pelleting layer coating the primary pelleting layer, the weight ratio of the three pelleting layers is 3 to 3 to 4, and the three pelleting layers integrally or respectively contain the seed coating agent containing 1.0% of soluble starch and the bonding agent containing 20.0% of water soluble starch. A processing method of the pelleted seed comprises the steps of respectively spraying the seed coating agent containing 1.0% of soluble starch and the bonding agent containing 20.0% of water soluble starch to the bonding agent and the seed coating agent in a nucleation granulation process, a primary pelleting process and a secondary pelleting process, so as to prepare the pelleted seed. The pelleted seed is inspected according to a condition that a reactant of the reaction between iodine and starch is blue. The processing method of the anti-faking pelleted seed is simple and safe, the anti-faking period of validity is long, the splitting performance of the pelleted seed can be improved, and the inspection method is simple and convenient, high in speed and low in cost.

The invention discloses a plasmid vector continuous lysis device. The device comprises a reaction container and a driving assembly, wherein the reaction container comprises a first container, a secondcontainer, a third container and a fourth container; the first container is used for containing a resuspension turbid solution containing thalli and a resuspension solution; the second container is used for containing a lysis solution; the third container is used for containing a neutralization solution, and the fourth container is used for containing a lysis turbid solution containing the resuspension turbid solution and the lysis solution; the driving assembly comprises a first driving pump, a second driving pump and a third driving pump; the first driving pump is used for pumping the resuspension turbid solution in the first container into the fourth container, and the second driving pump is used for pumping the lysis solution in the second container into the fourth container; and thethird driving pump is used for pumping the lysis turbid solution in the fourth container into the third container. According to the plasmid vector continuous lysis device disclosed by the invention, the stability of thallus lysis is ensured, and the thallus lysis time is shortened.

The invention discloses a method for accurately extracting DNA from wood targeted cells; through removal of exogenous pollution on a wood surface, in-situ detection of wood DNA, radial section of woodtissues, precise separation of the wood targeted cells enriched in DNA under an inverted microscope, cracking of wood cell walls, removal of a wood extract, DNA precipitation and adsorption, dissolution, purification, PCR amplification and other steps, DNA is extracted accurately from the wood tissues. The method aims at the wood targeted cells to carry out DNA accurate extraction, the quantity and purity of DNA extracted from the wood tissues are improved, and the interference of other types of cells of wood on DNA extraction is reduced. The method can be directly applied to study of molecular biology and provide a new technical support for identification of wood species.

The invention relates to the field of rubber cracking and recycling, in particular to a method for improving the efficiency of rubber cracking. The method crushes collected rubber products into blocks with a size close to the size of peanuts; Functional sealed tank, add thinner water to dissolve the rubber, and accelerate the rubber dissolution by stirring; connect the sealed tank directly to the rubber cracking device, keep a high degree of sealing, and perform high-temperature cracking and stirring on the dissolved rubber. Due to the high temperature, thinner The volatilization of water is separated from the solution; the volatilized thinner gas is collected and cooled for recycling; the dissolved rubber is more likely to react with the catalyst, and the heating is more uniform, which can effectively improve the cracking efficiency, and thinner is easy to volatilize and can be collected Cool and reuse.

The invention discloses a recombinant vector for constructing Saccharomyces cerevisiae lysing engineering bacteria and application thereof, belonging to the field of biotechnology. The recombinant vector of the present invention can knock out the SED1 gene in the Saccharomyces cerevisiae genome, and the obtained mutant strain can rapidly lyse the cells under the action of the lyase Zymolyase, and release the intracellular substances to the extracellular. The object of operation is Saccharomyces cerevisiae, which has wide applicability. There is no need to add additional reagents during the lysis process, and the cost is low. The lysis time is short, and the cells can be completely lysed within 2 hours, which greatly shortens the time for Saccharomyces cerevisiae cell lysis in the past. It is easy to operate, does not need cumbersome steps such as glass beads and the preparation of complex lysates, and only needs a simple buffer solution, which greatly simplifies the experimental process. Therefore, the Saccharomyces cerevisiae cracking method of the present invention can be used for enzyme activity detection, colony PCR, plasmid extraction, protein identification and high-throughput screening, etc., which has the advantages of simplicity, quickness and low cost, and has broad application prospects.

Provided is a method for tyre rubber pyrolysis, comprising the following steps: A, pretreating a tyre rubber; B, feeding the tyre rubber treated in step A into a composite pyrolysis chamber (2); C, heating a gas medium with a gas heating device (3) to a temperature of from 350°C to 700°C; D, continuously supplying the gas heated in step C to the composite pyrolysis chamber (2) and allowing the gas to act simultaneously with microwaves on the tyre rubber to conduct pyrolysis to obtain a solid product and a mixed gaseous product; E, subjecting the mixed gaseous product obtained in step D to oil-gas separation to remove a pyrolysis oil; and F, supplying part of the gases obtained in the oil-gas separation in step E to the gas heating device (3) described in step C and heating said part of the gases to a temperature of from 350°C to 700°C so as to recycle the same for reuse in the pyrolysis of step D. The method of the present invention can effectively reduce the energy consumption and time of pyrolysis and save costs.

The invention discloses an extracting method for RNA in serum exosomes. The extracting method is characterized by comprising the following steps that 1, the exosomes in serum are extracted; 2, the exosomes extracted in the step 1 are pyrolyzed through an RNA extracting reagent under the ultrasonic action; 3, the RNA of the exosomes pyrolyzed in the step 2 is separated into a water phase through chloroform; 4, the RNA obtained in the step 3 in the water phase is precipitated through isopropanol; 5, the RNA precipitated in the step 4 is washed through ethyl alcohol and then dissolved again, and the RNA in the serum exosomes is obtained. The extracting method for the RNA in the serum exosomes has the advantages of being short in extracting time, high in efficiency, good in effect and low in experimental cost.

The invention discloses a subcritical or supercritical water electrochemistry reinforced liquid phase catalytic biomass pyrolysis method. The method takes subcritical or supercritical water as a medium to process the biomass in presence of a catalyst, by electrolyzing water to produce hydrogen, the biomass is pyrolyzed and dissolved, and the solid biomass is decomposed and converted to low molecular weight biological oil; on one hand, the subcritical or supercritical water transfers heat to the biomass, on the other hand, free hydrogen is released under an electrolysis function, free radical chain reaction is timely blocked, yield of liquid fuel is effectively increased and quality is improved; the biomass is uniformly heated, a chemical bond of lignocellulose is ruptured, an ion pair intermediate is generated and is stimulated by an electric field, a pyrolysis rate is quickened, efficiency is high, a process is easy to control and a use rate of the biomass is improved.

The invention relates to degradable master batch, a plastic film comprising the same and a preparation method of the plastic film, and relates to the field of plastic processing, in particular relatesto the degradable master batch, the plastic film comprising the same and the preparation method of the plastic film. The degradable master batch can be degraded in various modes, and is high in degrading speed and high in efficiency. Raw materials of the degradable master batch contain the following components in percentage by weight: 8-12% of ferrocene, 72-76% of calcium carbonate, 4-8% of citric acid, 1-5% of a dispersant and 4-8% of a photosensitizer. The dispersant is prepared by compounding one or more of barium stearate, zinc stearate, calcium stearate, cadmium stearate, magnesium stearate and copper stearate. The degradable master batch accelerates the plastic film to be degraded multidimensionally while the degrading speed is increased, so that the degrading conditions of the plastic film are further broadened, and the degradable master batch can be degraded in various modes, and is high in degrading speed and high in efficiency.

The invention relates to the technical field of high-temperature pyrolysis of rubber, in particular to an environment-friendly high-temperature pyrolysis device for rubber, which includes a tank body, a climbing support mechanism, a sealing opening and closing mechanism, a vacuum pumping mechanism, a discharging mechanism, a stirring mechanism, and a third electromagnetic valve And an electric heating plate, the top of the tank is provided with a sealing opening and closing mechanism, and the side wall of the tank is provided with a vacuum mechanism, which can extract the tank to a certain degree of vacuum and reduce the melting point of the rubber. At the same time, the heating temperature can be reduced to reduce potential safety hazards. The sealing cover is connected with a stirring mechanism penetrating into the inside of the tank, which can increase the cracking speed and reduce the cracking time during the process of heating and melting the rubber. The top of the tank is equipped with a sealed opening and closing mechanism, and the side wall of the tank is connected with a climbing support mechanism, which is convenient for the staff to climb and load. And the support of the whole tank body is more stable and firm, preventing toppling over.

The invention discloses a carbon-ceramic brake part and a preparation method thereof. The carbon-ceramic brake part comprises silicon carbide, silicon and carbon fibers in a mass ratio of (40-60):(10-18):(25-35). The preparation method comprises the following steps: knitting or laminating the carbon fibers to form a carbon fiber blank; mixing polysilane, dibenzoyl peroxide and a silane coupling agent to form a silicon-containing precursor solution; dipping the carbon fiber blank into the silicon-containing precursor solution; after dipping is finished, taking out the carbon fiber blank, and solidifying and cracking sequentially in inert atmosphere, so as to obtain the carbon-ceramic brake part. The carbon-ceramic brake part prepared by adopting the preparation method has high friction coefficient, good braking performance, uniform component distribution and good performance consistency.

The present invention discloses a new glycolide preparation method, wherein glycolic acid condensation polymerization adopts heating dewatering in stages, and a lifting stirring method is used in a condensation polymerization reaction kettle to crush polyglycolic acid into powder. According to the present invention, the novel polyglycolic acid cracking kettle is designed, the polyglycolic acid melt is stirred in the cracking kettle in the vertical direction and in the horizontal direction through the elliptic spiral stirring device in the cracking kettle when the polyglycolic acid is subjected to heating cracking so as to effectively disperse the polyglycolic acid, such that the carbonization caused by the polyglycolic acid accumulation is avoided, and the glycolide yield is improved.

The invention belongs to the technical field of diamond coating preparation, and particularly discloses a method for improving the diamond coating deposition efficiency of hot filament CVD (chemical vapor deposition) based on magnetic confinement. Improved hot wire CVD equipment comprises a gas source, a coating chamber and a vacuum pump which are connected in sequence, wherein a base table and hot filament electrode assemblies are arranged in the coating chamber, and magnetic enhancement assemblies are arranged in the coating chamber. Each magnetic enhancement assembly comprises magnetic enhancement electrodes and a shared magnetic control power supply, and a spiral hot filament is connected between the magnetic enhancement electrodes. In the dissociation stage, the magnetic control power supply is controlled to introduce 0.3-0.5 A alternating current into the magnetic enhancement electrodes; in the deposition stage, the magnetic control power supply is controlled to introduce 3-5A alternating current into the magnetic enhancement electrodes; and in the cooling stage, the magnetic control power supply is controlled to supply 1-3A alternating current to the magnetic enhancement electrodes. By the adoption of the scheme, charged particle clusters do spiral motion under the action of the magnetic field, the residence time of particles is prolonged, the effective collision probability between the particles and the to-be-coated tool is increased, and then the deposition rate is increased.

The invention discloses a method for electrochemically enhanced liquid-phase catalytic low-temperature pyrolysis of biomass. The method uses ionic liquid as a catalytic reaction medium in the biomass pyrolysis process, so that the biomass is electrochemically stimulated in the ionic liquid to undergo a pyrolysis reaction. Generate high value-added pyrolysis products; the electrically assisted pyrolysis reactor used in the method of the present invention includes a cylinder, a spiral anode, and a grid cylindrical cathode. The spiral anode is arranged in the grid cylindrical cathode and The rotation is driven by a motor installed on the cylinder. The spiral anode and grid cylindrical cathode are arranged in the cylinder. The upper end of the cylinder is provided with a feed port and an air outlet. The lower part of the cylinder is provided with a discharge port and an ionic liquid. Inlet and air inlet; in the present invention, the biomass is heated evenly, the chemical bonds of lignocellulose are broken, ion pair intermediates are generated, and are stimulated by the electric field, speeding up the cracking rate, high efficiency, easy to control the process, and improving the utilization rate of the biomass .

The invention provides a solid organic material cracking method and system. The method comprises the following steps of: A, pre-treating a solid organic material; B, sending the pre-treated solid organic material into a cracking device; C, heating a gas medium to 350-700 DEG C; D, continuously importing the heated gas medium into the cracking device to act on the solid organic material with microwave, so as to carry out cracking to obtain a solid product and a mixed gaseous product; and E, importing a part of the obtained mixed gaseous product into a gas heating device in the step C so as to heat the mixed gaseous product to 350-700 DEG C to be circularly used for the cracking in the step D. The process can effectively decrease the cracking energy consumption and the cracking time, and can save the cost.

The invention relates to a derivative of a composition extracted from animal organs, wherein the animal organs are selected from livers, small intestines, large intestines and lungs, and animals are selected from cows, pigs, sheep and dogs.