100results about How to "Short experiment time" patented technology

The invention relates to a liquefied natural gas (LNG) drain diffusion and pool fire combustion simulated experimental platform and an LNG drain diffusion and pool fire combustion simulated experimental method. The platform comprises an experimental system, a testing system and a control system, wherein the experimental system consists of an LNG storage tank, heat-insulation pipes, experimental devices and an anti-explosion isolating wall; the testing system consists of an electronic balance, an ignition system, a methane concentration detector, a thermocouple tree, a data acquirer and a camera; and the control system consists of a programmable logic controller (PLC) system, various display instruments and a control valve. By designing an angle-adjustable LNG surface spreading experimental device with a changeable surface material, spreading and gasification diffusion experimental simulation of LNG on surfaces of different substances and at different flowing speeds can be carried out; and by matching the ignition system and the methane concentration detector, LNG gas cloud concentration change monitoring and gas cloud ignition can be realized, and data and images are acquired automatically. The platform has the advantages of short experimental time, low cost, high experimental efficiency and the like. By the design, experimental steps are less, experimental safety is guaranteed under the condition of severe monitoring and control, the platform is easy and convenient to operate, and experimental repeatability is high.

The invention relates to a test method and a test device for obtaining saturated solubility and diffusion coefficients of a solid solute in a liquid phase simultaneously. According to solute mass conservation law and Archimedes law in the process of dissolution, a physical model in the process of dissolving the solid solute is established and mathematical description of the physical model is determined so as to establish an experiment characterization method and a measurement device. In the test method and the test device, the saturated solubility and the diffusion coefficients of the solid solute in the liquid phase at a certain temperature and under certain pressure are calculated from the measured real-time dissolved amount through a specific inversion model (physical and mathematical model). The test device has the advantages of simple operation, short measuring time and capability of measuring large quantity of data; and the solubility and the diffusion coefficients are obtained simultaneously, and the measured data can be verified mutually, so the measuring accuracy is improved and the data consistency is ensured well.

The invention relates to a physical simulation huff-puff production experimental method and device. The method is characterized by comprising the steps that 1, a core model for an experiment is vacuumized; 2, simulation formation water is sucked into the core model till the core model absorbs water to reach saturation; 3, crude oil is injected into the core model till no simulation formation water is drained out of the core model; 4, according to the solution gas-oil ratio data of a target oil field to be measured and core model output simulation formation water volume, the volume of gas needing to be injected into the core model is worked out; 5, the gas is injected into the core model; 6, huff-puff fluid is injected into the core model, the core model is closed, and a soaking process is simulated; 7, after experiment time is up, the core model is opened for huff-puff fluid and crude oil spurt, and one-time physical simulation huff-puff production experiment is completed. Experiments in different huff-pull ways can be carried out, influence effects of different injection parameters or parameter quantized comparison effect data, and a reliable evaluation platform is provided for thickened oil field exploitation in different huff-puff ways.

The invention discloses a frictional wear testing machine for a hard alloy or a wear-resistant coating and a frictional wear testing method. The frictional wear testing machine comprises a motor, a frequency converter, a rack and an abrasive disc, wherein the motor is mounted below the rack and connected with the frequency converter; the abrasive disc is fixedly connected onto an output shaft of the motor in a sleeving manner by virtue of a screw hole in the center of a circle; moreover, a plurality of screw supporting arms are vertically arranged on the rack, supporting springs are mounted at the lower parts of the screw supporting arms in a sleeving manner, and loading springs are mounted at the upper parts of the screw supporting arms in a sleeving manner; and an upper clamping plate is horizontally arranged above the rack, fixedly clamped through the supporting springs and the loading springs and is used for adjusting the level and load through adjusting nuts at the tops of the screw supporting arms. The device disclosed by the invention can be used for adjusting the pressure by changing the elongation of each loading spring, and is simple in structure, low in construction cost, suitable for a frictional wear test on the hard alloy or the surface wear-resistant coating, short in experimental time and saved in time and labor.

The invention belongs to the technical field of immunohistochemistry, and in particular relates to a histological classification immunohistochemical multiple staining detection method for lung cancer. The primary antibody in the detection method is various mixed primary antibodies. In order to overcome the defects that existing lunge cancer is not easy to classify in histology, and detection based on an immunohistochemical technology consumes time and wastes labor, the staining process is simple and quick by adopting the method, so that more abundant information with strong contrast can be obtained on a same slice quickly and conveniently. According to the invention, the mixed primary antibodies and various detection amplifying systems are applied to identifying histological classification of lung cancer, so that not only can the same sensitivity and specificity of each antibody in the mixed antibodies be maintained in comparison with those in simple staining, but also the method has the advantages of less staining steps, short experimental time, high stability, intuitionistic comparison of experimental result, far great information amount than single staining and the like, especially has remarkable advantages of biopsic pathological diagnosis with small specimen amount.

The invention discloses a hypersonic inward turning inlet and isolator flow field chromatogram display method and system. The obtaining method comprises the steps of inputting water vapor into a wind tunnel, and forming nano-particles via condensation of the water vapor in the wind tunnel; the nanoparticles entering, as tracer particles, into a flow field formed by an inward turning inlet and an isolator; and irradiating a plurality of cross-sectional positions in at least one direction of the flow field with laser light, and shooting a scattering image of the tracer particles in the laser irradiated plane to obtain a tomographic image of the flow field. In the invention, the nanoparticles condensed by the water vapor are used as tracer particles to analyze the scattering image, the flow field density distribution is indirectly obtained, the flow field observation can be well realized, and the problem that a traditional schlieren method is incapable of accurate reflection is solved. In addition, the tracer particles formed by the water vapor easily meet the requirements of the number of particles and diameter, the injection process of the nanoparticles is reduced, and the method is not limited by the time of a wind tunnel experiment.

The invention discloses a contact thermal resistance testing system with variable pressure and-temperature in a profound hypothermia area. The contact thermal resistance testing system comprises a testing device, a pressure loading device, a heating and temperature measuring device and a refrigerating device; the testing device comprises a vacuum cavity, a thermal insulation multilayer material, an anti-radiation cold shield, a solid thermal insulation material, a sample clamp, a testing platform and a vacuum cavity bottom plate; the pressure loading device comprises a pressure sensor, an airinlet pipeline, a pressure-bearing corrugated pipe and a thermal insulation screw rod; the heating temperature measuring device comprises a heating plate, a temperature sensor and a heating wire; therefrigerating device comprises an oxygen-free copper cold head, a refrigerating machine and a compressor; and the pressure loading device, the heating and temperature measuring device and the oxygen-free copper cold head are mounted in the vacuum cavity, the pressure-bearing corrugated pipe is fixed to the oxygen-free copper cold head and the solid thermal insulation material through the thermal insulation screw rod, the refrigerating machine is mounted on the vacuum cavity bottom plate, and all the devices are tightly connected through screws. The contact thermal resistance testing system with the variable pressure and-temperature in the profound hypothermia area has the advantages of easy operation, small temperature fluctuation, high operability, short test time, high precision and thelike.

The invention relates to a quantitative detection instrument and method for cashmere fiber and wool fiber blending. A micro-gloss meter, a microscope and a digital camera are mounted on a three-dimensional displacement automatic control stage. The stage realizes automatic and precise action and can be positioned The fiber photographed by the microscope corresponds to the fiber photometry measured by the micro-gloss meter; the micro-gloss meter can accurately measure the gloss of each fiber; the computer and image processing system adopts advanced image processing technology to accurately measure, Calculate the fiber diameter and scale density; the information processing system comprehensively evaluates the data collected about fiber diameter, gloss, fiber axial uniformity and scale density, and accurately judges the fiber composition. The invention adopts high-resolution image real-time capture, two-dimensional workbench automatic feed, automatic continuous rapid measurement, improves work efficiency, has high degree of automation and intelligence, simple operation, and changes the differences in test results caused by human factors.

The invention relates to a material formaldehyde emission rate limit load rate test method which comprises the steps: 1, a test cabin is cleaned and dried until the formaldehyde concentration is smaller than or equal to 0.006mg / m<3>; 2, a test sample is pretreated and arranged on a sample frame of the test cabin, and the test cabin is sealed and recorded as an experiment zero point; 3, gas in the test cabin is collected, the formaldehyde concentrate in the test cabin is recorded, and the sampling time is recorded; 4, the specific value between the formaldehyde concentration and the limit load rate is utilized as a longitudinal coordinate, the sampling time is utilized as a horizontal coordinate, a test curve is drawn, the least squares fit is performed, and the slope of the test curve is the formaldehyde emission rate of the test sample. According to the test method, a limit load rate method is utilized to test a free emission process of a material, and the emission property of the material can be truly reflected; the limit load rate material use amount is small, the cost is savedreduced, and the test results are true and conform to the reality; the experiment time is shortened to 2 to 5h; the sealed test cabin is utilized, so that the situation that a lot of clean air flows into is avoided, the disturbance air error is avoided, and the experiment accuracy is high.

The invention provides a hot-pressing composite testing method based on a thermal simulation test machine. The method comprises steps as follows: S1: to-be-composited material test pieces are prepared: two to-be-composited materials are processed into cylindrical test pieces, oxidation layers on composite surfaces of the to-be-composited materials are removed, and the composite surfaces are washed clean; S2: the test pieces are clamped: the two to-be-composited test pieces are clamped on clamping devices of the thermal simulation test machine respectively, and the clamping devices are moved to mutually contact with the composite surfaces of the two to-be-composited test pieces; S3: the two to-be-composited test pieces are heated and kept at the constant temperature; S4: heating current is interrupted, and the two to-be-composited test pieces are drawn away; S5: the two to-be-composited test pieces are compressed, and a composite test piece is obtained. The test method is simple and easy to implement when used for a hot-pressing composite test, and influences of resistance welding and diffusion welding caused by impulse current when the thermal simulation test machine performs heating and thermal insulation on the test pieces on final composite performance of the test piece are eliminated.

The invention relates to an LNG leakage water curtain inhibition experiment method which aims at mainly solving the problem of the blank of the LNG leakage water curtain inhibition experiment method in the prior art. According to the technical scheme adopted by the invention for well solving the problem, due to the adoption of the LNG leakage water curtain inhibition experiment method, LNG leakage water curtain inhibition experiment is implemented by virtue of an LNG leakage water curtain inhibition experiment device; the experiment method comprises the following steps: (1) guiding LNG in an LNG storage tank into a leakage tank; (2) opening a leakage nozzle and spraying the LNG out of the leakage nozzle; (3) starting a data acquisition system, carrying out data acquisition and recording image; (4) starting a water curtain system, spraying water out of a water curtain nozzle to form a water curtain, and carrying out data acquisition by virtue of the data acquisition system and recording the image; and (5) sequentially closing the leakage system, the water curtain system and the data acquisition system after the data collected by a monitoring point array is stable so as to end the experiment. The method can be applied to the LNG leakage water curtain inhibition experiment.

The invention discloses a modeling method of a helicobacter pylori infection animal model. The modeling method comprises the steps of: 1, by using a 4-weeks old female C57BL / 6 mouse as the experimental animal, performing intra-gastric administration for 3 to 7 days by mixed antibiotic, wherein the mixed antibiotic is composed of gentamicin, vancomycin and nystatin; 2, raising the C57BL / 6 mouse which has been subjected to the intra-gastric administration in the step 1, for 6 to 8 days; 3, inoculating helicobacter pylori: fasting the C57BL / 6 mouse obtained in the step 2, carrying out intra-gastric administration by helicobacter pylori, and feeding after 8 to 12 hours, wherein the inoculating in the step 3 is performed one time every other day, and performed 2 to 4 times in total; and 4, raising the mouse which has been inoculated for 2 to 4 times in the step 3. In the modeling method provided by the invention, the experimental time is short, the operation time is less, the order of magnitude of the helicobacter pylori infection is high, and the infection time is endurable; the model can be widely used for the method of helicobacter pylori infection animal in related researches.

The invention provides a parameter optimization method for an antenna in a wireless cellular network. The method comprises the steps of: selecting a horizontal angle and a downward inclination angle of the base station antenna in the wireless cellular network as parameters, determining an iteration initial angle value of each parameter, and determining an iteration step length of each parameter; according to the iteration initial angle value and the iteration step length of each parameter, carrying out repeated wireless network communication experiments by the iteration process, and calculating and storing an overall fitness value of a cell and an angle value of each parameter in each time of iteration; and when the fitness values accord with a preset convergence threshold, determining anoptimal angle value of each parameter in the iterations corresponding to the fitness values which accord with the convergence threshold as an optimized angle value of the parameter. According to the method provided by the invention, by adopting an iteration algorithm, a convergence speed is high, experiment times are few, experiment time is short, the optimization process does not depend on selection of initial parameter values, and an obtained optimal angle combination enables an antenna signal to completely cover an overall region.

The invention relates to a mathematical probability demonstration device. The mathematical probability demonstration device comprises a bottom seat plate, a fixing plate is arranged at the upper end of the bottom seat plate, connecting plates are arranged on the left side and the right side of the front end of the fixing plate, a support plate is arranged at the upper ends of the connecting plates, support rods are arranged on the right sides of the upper end of the support plate, a placing part is arranged at the upper end of the support rods, the placing part is internally provided with a throwing part, mounting holes are formed in the upper end and the lower end of the throwing part, a first pipeline is arranged on the outer side of the lower end mounting hole, a second pipeline is arranged at the left end of the first pipeline, one end of a first elbow pipe is arranged at the left end of the second pipeline, the other end of the first elbow pipe is provided with a third pipeline, one end of the second elbow pipe is arranged at the right end of the third pipeline, one end of a fourth pipeline is arranged at the other end of the second elbow pipe, a fifth pipeline is arranged atthe other end of the fourth pipeline, a first shunting pipe is arranged at the lower end of the fifth pipeline, sixth pipelines are arranged on the left side and the right side of the lower end of thefirst shunting pipe, seventh pipelines are arranged at the lower ends of the left sixth pipeline and the right sixth pipeline, second shunting pipes are arranged at the lower ends of the left seventhshunting pipe and the right seventh shunting pipe, and out-guiding pipes are arranged on the left sides and the right sides of the lower ends of the second shunting pipes.

Disclosed is a loading unit for simulating vehicle dynamic load on pavement, wherein the road leveling machine is provided with a mounting rack and a frame, the frame is provided with a directional selecting valve for connecting the hydraulic pump through tubes, a loading unit for simulating vehicle dynamic load on pavement is arranged on the mounting rack, a hydraulic fluid motor is arranged on the loading unit for connecting the directional selecting valve through piping. In accordance with the invention , the vertical dynamic load to the pavement is employed to simulate the impulsion impact load to the pavement by the automobile during high speed running, thus analyzing the influence of the subgrade settlement to asphalt concrete road destruction. The unit can shorten experiment period and increase the test efficiency.

The invention relates to a test platform for integrated performance of clutch, which comprises a control platform and a test platform; the control platform comprises a display screen, a host machine, a test instrument and a distribution box; the test platform comprises a protection device, hot air blowers arranged at the both sides of the protection device, and a fan; the fan is connected with a mechanical transmission device; the mechanical transmission device comprises a rack and a motor disposed on the rack; and the output end of the motor is in turn provided with a transmission shaft, a supporting rack, a fastening piece and a torque sensor. The test platform has high test accuracy.

The invention provides mechanochromic high-toughness nylon 6 and a preparation method thereof. The preparation method comprises the following steps: firstly, preparing an isocyanate-terminated polyurethane prepolymer; then, enabling excessive terminal isocyanate groups to react with hydroxyl groups of spiropyrane chromophore; adding an isocyanate-terminated spiropyrane derivative into caprolactammelt, carrying out an addition reaction on isocyanate and caprolactam, and finally injecting the mixture into a mold to carry out an anionic polymerization reaction to obtain the mechanochromic high-toughness nylon 6. According to the invention, the raw material ratio and reaction conditions are reasonably controlled, polyurethane chain segments and spiropyrane chromophore are introduced into a polyamide molecular chain, the position relation and molecular weight between the chain segments are reasonably regulated and controlled, the effectiveness of external force transmission of the preparedmechanochromic high-toughness nylon 6 is remarkably improved, and then the sensitivity of mechanochromic response of the spiropyrane chromophore is remarkably improved.

The invention relates to a sequencing library construction method for detecting lentivirus insertion sites, and a lentivirus insertion site detection method. The sequencing library construction method comprises the following steps: extracting genome DNA from lentivirus infected cells; carrying out fragmentation on the genome DNA and processing the genome DNA into a form suitable for linker connection; connecting asymmetric double-chain connectors to two ends of the fragmented genome DNA, wherein the asymmetric double-chain connectors comprise a long-chain sequence and a short-chain sequence; carrying out first-round PCR amplification on a joint connection product; performing second-round PCR amplification on a product of the first-round PCR amplification; and cyclizing the product of the second-round PCR amplification to obtain a cyclized sequencing library suitable for computer sequencing. The methods have the characteristics of simplicity in operation, short experiment time, low cost, small initial quantity, high flux, multiple analyzable aspects and the like, and can better perform accurate analysis and evaluation on the virus insertion site in gene therapy.

The invention discloses a single-pipe-structure fixed bed reactor for teaching experiment. The fixed bed reactor is characterized in that a reaction pipe is arranged in a barrel; a stainless steel mesh and filler are arranged under the reaction pipe; heating devices at two sides of the barrel are used for carrying out heating reaction. The reactor is used for teaching experiment equipment, the structure of the reactor is greatly simplified, the reactor is easy to assemble and disassemble, and the cost of a teaching reactor experiment is reduced, and in addition, an internal cavity of the reactor is small in volume, so that the standing time of a gas phase raw material and a product in the reactor is reduced, the side reaction is reduced, and meanwhile, the conversion rate of the raw material is improved.

The invention relates to the field of biology, in particular to an isolated culture method for primary canine vascular endothelial cells. The isolated culture method comprises the following steps: S1,taking canine aorta; S2, turning over the canine aorta and cleaning; S3, obtaining vascular endothelinal surface single-layer cells; and S4, obtaining the primary canine vascular endothelial cells and performing in-vitro culture. The isolated culture method disclosed by the invention has the advantages that: operations are simple, repeatability is good and experimental time is short; the vascularendothelial cells are derived from canine aorta dead of a car accident, so that ethical arguments are avoided; cells are scraped through a mechanical method, and are combined with a collagenase digestion method, so that a great number of vascular endothelial cells with strong multiplication capacity can be obtained; the obtained vascular endothelial cells are high in purity, and are very low in cell pollution probability. A turnover operation success rate is nearly 100%; and connective tissues adhered on the vascular surfaces are stripped off, so that the pollution chance of other cells suchas fibroblast is greatly reduced.

The present invention relates to a reporter gene assay for measuring biological activity of recombinant human keratinocyte growth factors. The method utilizes plasmids comprising a SRE response element and overexpressed lentiviruses comprising a KGFR2IIIb gene to respectively transfect and infect HEK293 and HaCat cells to obtain a cell line stably expressing the SRE and KGFR2IIIb, after the cell line is stimulated by recombinant KGF-1 / KGF-2, an expression of a downstream luciferase reporter gene of a SRE-containing promoter, and a four-parameter curve is fitted according to reporter gene signal values to determine the biological activity of the recombinant KGF-1 / KGF-2. The rapid, sensitive and accurate quantitative detection method is established aiming at the biological activity measurement of the KGF, is short in test period and simple in operation, and has important guiding roles for quality controls in research, development and production of the recombinant KGF-1.

The invention relates to the technical field of travel survey, in particular to a high-voltage motor insulation detection method and device. The method comprises the following steps: a first experiment for detecting the surface conductivity fouling of the high-voltage motor coil; and / or a second experiment for detecting the aging degree and dielectric loss of the insulating layer of the high-voltage motor coil; and / or a third experiment for detecting the partial discharge amount in the insulating layer of the high-voltage motor coil. All data are collected within the rated voltage range of thehigh-voltage motor, and the experiment is reliable and stable; no harm is caused to client equipment; the operation is convenient and easy; and the experiment time is short, and the whole experimentprocess is within one hour.

The invention discloses a specific molecular marker DNA sequence of lactobacillus casei, application of the specific molecular marker DNA sequence and a detection method of the lactobacillus casei. The specific molecular marker DNA sequence is shown as SEQ ID NO.6. the detection method comprises the steps of 1 extracting a genome DNA of a sample to be detected, 2 using the genome DNA obtained in the step 1 as a template and using a semi-random primer represented I the SEQ ID NO.1 as an amplification primer to perform single primer PCR amplification, 3 conducting clone sequencing on an amplification product and comparing a sequencing result with the sequence represented by the SEQ ID NO.6, wherein it is shown that the sample to be detected contains the lactobacillus casei if the homology degree is higher than 99%.

The invention provides a prediction method for a vibration signal stable value of a wind tunnel balance, and belongs to the technical field of signal analysis. The method comprises: 1, from many-body dynamics point of view, simplifying a system to a four-freedom-degree dynamical model, performing dynamics modeling on each degree of freedom; 2, preliminarily intercepting a segment of data from a balance measured signal, the data segment being divided into three processes of flow field, stable vibration, and smooth and steady state; 3, selecting signal troughs in the above data segment as starting time and cut-off time, so a truncated signal including multiple periods; 4, performing Fourier transform on the truncated signal, extracting main frequency of the signal; 5, after the main frequency is obtained, based on an attenuation function model obtained in the first step and a gradient descent method, fitting and predicting a truncated signal curve; 6, in order to realize automatic interception of a vibration signal period, and extracting the main frequency of the vibration signal, analyzing relations between a cycle number and signal precision. The method is mainly used for predicting a vibration signal stable value of a wind tunnel balance.

A rock core clamper used for carbon dioxide corrosion tests is provided. The rock core clamper comprises a clamping cylinder formed by connecting a first half cylinder to a second half cylinder. A corrosion cavity is formed in the clamping cylinder. Two ends of the clamping cylinder are respectively provided with a first opening. The cylinder wall of the clamping cylinder is also provided with two axisymmetric second openings. The rock core clamper can perform carbon dioxide corrosion, and perform permeability tests, a rock core does not need to be taken out repeatedly in a process and all tests are completed in the rock core clamper. As the rock core is not taken out, the position of the rock core does not change, corrosion can be continued after permeability calculation is finished, thus achieving continuous value measuring, making experiment time short and saving the rock core.

The invention discloses a tilapia cold-resistant performance determination method, and belongs to the technical field of seed selection of a tilapia cod-resistant kind. The method comprises the following steps: 1, selecting tilapias: 2, performing low-temperature-induced death processing; 3, calculating a low-temperature semilethal temperature; 4, performing temperature-return processing; 5, calculating a low-temperature death rate; and 5, carrying out a cold resistance evaluation. The determination method provided by the invention is simple, is easily implemented, is accurate and reliable, and can effectively determine and evaluate the cold resistance performance of the tilapias.

The present invention relates to a bursaphelenchus nematode culture and collection method and a device thereof, pertaining to the technical field of nematode culture. The device comprises a tissue culture bottle (1), a tissue culture bottle cover (2) and an eccentric pipe body which is embedded on the tissue culture bottle cover (2) and consists of an eccentric pipe cover (3) and an eccentric pipe (4). The method is improved on the basis of the conventional culture and collection method. Compared with the prior art, the present invention has the advantages of simple structure, simplifying the complex operating procedures of the conventional nematode culture and experimental nematode selection, lowering the experimental difficulty, saving the experiment time, improving the purity of experimental nematode and the accuracy of experimental results, etc. The method and the device of the present invention can be widely applied to the culture of bursaphelenchus nematode and the collection of high-purity nematode group.