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Tilapia cold-resistant performance determination method

A determination method and technology for tilapia are applied in the field of determination of cold resistance of tilapia, which can solve the problems of large differences, poor accuracy, long time, etc. short effect

Inactive Publication Date: 2016-01-20
GUANGXI ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the low temperature stress experiments reported by different studies do not have a fixed mode of cooling and heating. Different experimental designs have different parameters such as holding time, initial temperature, cooling rate and minimum temperature. The research results are quite different, and the repeatability of the test is poor. The cold resistance performance index of tilapia is compared with the cold resistance ability, and the accuracy is poor.

Method used

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  • Tilapia cold-resistant performance determination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Choice of tilapia:

[0033] The 30-day-old American strain Olia tilapia (hereinafter referred to as "American Olia") and the Egyptian strain Nile tilapia were selected from the national Guangxi Nanning tilapia breeding farm. (hereinafter referred to as "Egyptian Nile") and 180 hybrid Neo tilapia (hereinafter referred to as "Hybrid Neo"), were first raised in a 1m×1m×1m tank for 7 days, and then randomized using a single factor test. Divided into 3 test groups, 180 fish in each test group, 3 replicates in each group, 60 fish in each replicate, put into 9 refrigerated aquariums with specifications of 50cm×60cm×80cm respectively, after 24 hours of environmental adaptation, That is, the tilapia used in the experiment was obtained.

[0034] (2) Lethal treatment by low temperature stress:

[0035] The initial water temperature of the refrigerated aquarium was set at 20°C, and indoor artificial vertical cooling was adopted to cool down to the final water temperature of 8...

Embodiment 2

[0058] (1) Choice of tilapia:

[0059] The zero generation (P 0 ), the first generation (P 1 ) and the second generation (P 2 ) of American Oria tilapia (hereinafter referred to as "American Oria"), 45 each, were first raised in a 1m × 1m × 1m tank for 5 days, and were randomly divided into 3 test groups by single factor test. There were 45 fish in each test group, 3 replicates in each group, and 15 fish in each replicate. They were put into 9 refrigerated aquariums with specifications of 50cm×60cm×80cm respectively. After 24 hours of environmental adaptation, tilapias for testing were obtained.

[0060] (2) Lethal treatment by low temperature stress:

[0061] The initial water temperature of the refrigerated aquarium was set at 22°C, and indoor artificial vertical cooling was adopted to cool down to the final water temperature of 7°C at a cooling rate of 0.3°C / 3h until all tilapias used in the test in step (1) died.

[0062] (3) Calculation of low temperature semi-lethal ...

Embodiment 3

[0078] (1) Choice of tilapia:

[0079] American Nile tilapia (referred to as "American Nile") and Thai strain Nile tilapia (referred to as "Thailand") were selected from the national Guangxi Nanning tilapia breeding farm. Nile") and Egyptian strain Nile tilapia (referred to as "Egyptian Nile"), each with 60 fish, was first raised in a 1m×1m×1m large tank for 10 days, and was randomly divided into 3 test groups by single factor test. 60 fish in each test group, 3 repetitions in each group, 20 fish in each repetition, put them into 9 refrigerated aquariums with a size of 50cm×60cm×80cm, and after 24 hours of environmental adaptation, the tilapia for the test can be obtained .

[0080] (2) Lethal treatment by low temperature stress:

[0081] The initial water temperature of the refrigerated aquarium was set at 25°C, and indoor artificial vertical cooling was adopted to cool down to the final water temperature of 9°C at a cooling rate of 0.1°C / 3h until all tilapias used in the t...

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Abstract

The invention discloses a tilapia cold-resistant performance determination method, and belongs to the technical field of seed selection of a tilapia cod-resistant kind. The method comprises the following steps: 1, selecting tilapias: 2, performing low-temperature-induced death processing; 3, calculating a low-temperature semilethal temperature; 4, performing temperature-return processing; 5, calculating a low-temperature death rate; and 5, carrying out a cold resistance evaluation. The determination method provided by the invention is simple, is easily implemented, is accurate and reliable, and can effectively determine and evaluate the cold resistance performance of the tilapias.

Description

technical field [0001] The invention relates to a method for measuring the cold-resistant performance of tilapia, belonging to the technical field of tilapia cold-resistant variety breeding. Background technique [0002] Native to Africa, tilapia is a warm-water fish with the characteristics of fast growth, strong adaptability, and tender meat. It is the leading freshwater species in my country and is of great significance to my country's export earnings. However, tilapia has poor cold resistance, and its lethal temperature is 7-12°C. In recent years, due to the continuous deterioration of the environment, the increase in extreme weather, and the long duration of low water temperature, incidents of tilapia being frozen to death during the winter have occurred from time to time, which has caused huge economic losses to the tilapia aquaculture industry and seriously hindered The sustainable and healthy development of the tilapia industry has been ensured. Therefore, it is ve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81A01K61/00
Inventor 林勇宾石玉杜雪松
Owner GUANGXI ACADEMY OF FISHERY SCI
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