71results about How to "Rapid inactivation" patented technology

A method for electrolytically generating a biocide having an electron deficient carrier fluid and chlorine dioxide, including providing a carrier fluid; providing a pair of electrodes interposed by a semi-permeable membrane within a vessel for creating a first passageway and a second passageway, an anode electrode of the pair of electrodes disposed in the first passageway, cathode electrode of the pair of electrodes disposed in the second passageway; flowing the carrier fluid through the vessel; applying an electric potential to the pair of electrodes to produce an oxidative acidic fluid, a reductive alkaline fluid, and anodic gases in the container; removing the fluids and gases from the vessel; mixing a portion of the anodic gases with the reductive alkaline fluid to produce a hypochlorite solution; and mixing a chlorite brine with the hypochlorite solution, followed by the introduction of additional oxidative acidic fluid to release the biocide.

Provided are a super amphiphilic molecule emulsifier with responsiveness and an emulsion and a preparation method thereof. The emulsifier is prepared from polyetheramine and long-chain fatty acid, and every 100 parts of the emulsion is prepared from, by weight, 49.5-80 parts of aqueous phase, 1-3 parts of the emulsifier and the balance oil phase. Polyetheramine and long-chain fatty acid are mixed to serve as the emulsifier, the emulsifier, the aqueous phase and the oil phase are mixed and stirred to be uniform, and the emulsion is formed. A demulsification method of the emulsion comprises the steps that CO2 is fed into the emulsion, or acid or n-caprylic acid or n-hexylic acid are dropwise added into the emulsion, and standing is conducted after shaking is conducted. The emulsifier has the good pH value or CO2 responsiveness; on one hand, the emulsion stabilized through the emulsifier has long-term stability; on the other hand, the pH value is adjusted by adding the acid or feeding CO2, so that rapid inactivation of the emulsifier is achieved, emulsion demulsification is achieved, the cost is low, and the adaptability is wider.

The present invention discloses an RNA virus inactivation preservation solution. The inactivation preservation solution comprises ammonium salt, 1-ethyl-3-methylimidazolium bisulfate, lithium chloride, ethylenediaminetetraacetic acid, an RNase inhibitor, a surfactant, a reducing agent, a buffer agent and water. The present invention also provides an RNA virus sample inactivation and preservation method and an RNA virus detection method, and the method is conducted by using the inactivation preservation solution. The preservation solution has low cost, inhibits RNase activity, preserves RNA integrity, realizes preservation and transportation of samples at room temperature, can also quickly inactivate pathogens at room temperature, reduces infection risks of medical personnel, and reduces false negatives in detection. At the same time, the inactivation preservation solution can realize a lysis function, does not contain chaotropic reagents and alcohols, is compatible with mainstream nucleic acid extraction kits on the market, greatly saves time of an entire process, and is particularly suitable for 2019 new coronavirus (COVID-19) inactivation and lysis steps after swab sampling.

The invention relates to a process for the alkylation of benzene with isopropanol (IPA) as alkylating agent, or blends of isopropanol and propylene, which comprises effecting said reaction completely in gaseous phase and in the presence of a catalytic system containing a zeolite belonging to the MTW family.

A method for electrolytically generating a biocide having an electron deficient carrier fluid and chlorine dioxide, including providing a carrier fluid; providing a pair of electrodes interposed by a semi-permeable membrane within a vessel for creating a first passageway and a second passageway, an anode electrode of the pair of electrodes disposed in the first passageway, cathode electrode of the pair of electrodes disposed in the second passageway; flowing the carrier fluid through the vessel; applying an electric potential to the pair of electrodes to produce an oxidative acidic fluid, a reductive alkaline fluid, and anodic gases in the container; removing the fluids and gases from the vessel; mixing a portion of the anodic gases with the reductive alkaline fluid to produce a hypochlorite solution; and mixing a chlorite brine with the hypochlorite solution, followed by the introduction of additional oxidative acidic fluid to release the biocide.

The invention belongs to the field of a processing technology of an aquatic condiment and particularly relates to antarktischer krill shrimp sauce capable of being rapidly produced by an enzyme method. The antarktischer krill shrimp sauce is a novel shrimp sauce product obtained by taking antarktischer krill as a raw material and sufficiently utilizing the characteristic of high autolytic enzyme activity through steps of unfreezing, defluorinating, taking meat, crushing, self-dissolving, carrying out two sections of enzymolysis, adding salt and carrying out enzymolysis, blending, sub-packaging, sterilizing and carrying out enzyme destruction. The antarktischer krill shrimp sauce produced by the enzyme method has important characteristics in the aspects of production, such as short period and low cost; the product has the characteristics of characteristic red color of krill shrimp meat, moderate viscosity, uniform texture, abundant nutrients, unique flavor, low salt content and short production period; in the shrimp sauce, the content of proteins accounts for more than or equal to 10% and the content of amino-state nitrogen is higher than 1.1g / 100g (according to the content of N); the content of total volatile basic nitrogen (TVBN) is lower than 50mg / 100g; and defluorination treatment is carried out so that the content of fluorine in the antarktischer krill shrimp sauce is lower than 70mg / Kg. The shrimp sauce provided by the invention can be cooked and directly eaten and can also be used for producing preserved vegetables with a special flavor.

The invention relates to a rapid pulping process. The method comprises the following steps: heating water and soybeans to boiling and maintaining the boiling state to first inactivate anti-nutritional factors in soybeans; crushing soybeans, mixing the crushed soybeans and water to form soybean milk, and inactivating anti-nutritional factors in soybean milk again. Compared with the prior art, the rapid pulping process of the present invention saves the boiling step, and the softening of soybeans improves the crushing efficiency and shortens the crushing time, thus shortening the entire pulping cycle to a large extent and satisfying people's fast-paced life .

The invention provides a reagent composition for stabilizing nucleic acid molecules in a biological sample. The reagent composition comprises a pH stabilizer, a chelating agent, a protein denaturant, an antimicrobial agent and optional inorganic salt ions, a tissue penetrant, a reducer, a nuclease inhibitor and the like. The invention also provides a method for transporting, storing and stabilizing the nucleic acid molecules in the biological sample by using the reagent composition and application of the reagent composition in transporting, storing and stabilizing the nucleic acid molecules in the biological sample. The reagent composition ensures that high molecular weight DNA in the biological sample is kept for a long time at normal temperature.

The present invention relates to industrial sulfur tolerant catalyst for vapor ethylation of coking benzene to prepare ethyl benzene and its preparation process. The catalyst consists of nanometer HZSM-5 zeolite molecular sieve, alpha-Al2O3, and metal or non-metal oxide as modifier. Its preparation process includes high temperature water vapor or ammonia water vapor treatment of 20-200 nm crystal grain size HZSM-5 zeolite molecular sieve catalyst; and soaking the molecular sieve catalyst to support oxide of IIB element zinc, oxide of IIIB element La, oxide of VIB element Microwave oven and / or oxide one non-metal element P for modification. It may be used in the vapor ethylation of coking benzene with sulfur content of 200-800 preparation directly without needing desulfurizing and with the catalyst regenerating period as long as 60 days and other advantages.

A water treating and disinfecting process for water works includes such steps as adding chlorine in water for disinfecting water by free cl ions for ions for 5-30 min, and adding ammonia to convert said free cl ions to chloroamine for disinfecting water further.

The invention belongs to the field of fermentation engineering, and particularly relates to marine low-temperature glucose oxidase and application thereof to the preservation of a sea product. The oxidase is originated from Penicillium crustosum (GenBank accession number MG983981). The marine low-temperature glucose oxidase is applied to the preservation of the sea product. A preservation method comprises the following steps of firstly impregnating the sea product needing the preservation for 2min to 20min in mixed liquor of a marine low-temperature glucose oxidase solution and a glucose solution, and then putting into a preservation box to carry out low-temperature preservation after impregnation, wherein the concentration of the marine low-temperature glucose oxidase solution is 10mg / L to 60mg / L; the concentration of the glucose solution is 1mg / L to 15mg / L; the mixing volume ratio of the marine low-temperature glucose oxidase solution to the glucose solution is 1 to (1 to 3). The low-temperature glucose oxidase provided by the invention is more high-efficiency in deoxygenization in a low-temperature condition; the oxidization of nutritional ingredients of protein, fat and the like can be effectively prevented, and the chemical preservation with a potential safety hazard is replaced.

The present invention discloses a preparation method of low oxalic acid ultrafine green tea powder. The preparation method of the low oxalic acid ultrafine green tea powder is as follows: fresh tea leaf raw materials are directly soaked using boiling water; polyphenol oxidase in the fresh tea leaves is inactivated; at the same time, oxalic acid in the fresh tea leaves is dissolved out; and then ultrafine homogenization, sterilization and drying into powder are conducted. The content of the oxalic acid in the ultrafine green tea powder is greatly reduced; the fineness of the tea powder can reach 1,000 meshes to 3,000 meshes; the ultrafine green tea powder is bright green in color and luster, and both excellent in nutrients and mouthfeel, and can also enable populations susceptible to calculus to very well enjoy the high-quality ultrafine green tea powder.

The invention provides a method for extracting and separating ginkgo flavone and ginkgolide from ginkgo leaves. The method comprises the three steps of an enzyme deactivation process, an extraction process and a column purification process. According to the method, fresh ginkgo leaves are cooked through alkaline water for enzyme deactivation, and enzymes in the raw material of the fresh ginkgo leaves can be rapidly deactivated; by means of alkaline water soaking, the ginkgo flavone in the raw material can be dissociated out to the maximum extent, residual alkaline water after cooking is added to organic solvent for extraction, subsequent complete extraction is facilitated, and the product yield is increased.

A sputum treating liquid for inspecting standard films of Mycobacterium tuberculosis in sputum comprises ethyl alcohol, isopropyl alcohol, boric acid, disodium EDTA, SDS, a mucus dissolving agent and distilled water and is capable of dissolving phlegm, deactivating pathogenic bacteria such as Mycobacterium tuberculosis as well as viruses in sputum, preventing corrosion and splitting exfoliative cells in sputum. Applied with simple centrifugal operation, the sputum treatment with the sputum treating liquid enables 8 to 10 spits of sputum to be treated at a time, and even sputum left for 24 hours, the treatment quantity of sputum is increased, standard operation is achieved through a standard automatic film-making technique, the operation is simple and easy, detection sensitivity is improved, detection is accurate and reliable, and the sputum treating liquid is suitable for the general investigation of tuberculosis high-risk groups, screening of rural tuberculosis and clinical diagnosis of tuberculosis.

The invention discloses a method for preparing a high light oil selectivity hydrocracking catalyst. The method comprises the following steps: carrying out chemical treatment on an ultra-stable Y molecular sieve to clean off amorphous aluminum in pores, carrying out thermal treatment, preparing a carrier from pseudo-boehmite and the amorphous aluminum, further carrying out metal element soaking andsupporting, thereby obtaining the high light oil selectivity hydrocracking catalyst. By using a gentle chemical method, residual amorphous aluminum in pores of a molecular sieve, then diffusion of aprimary cracking product can be promoted, and a framework structure is not affected; secondly, the pseudo-boehmite with high glue dissolubility is kneaded with the amorphous aluminum, so that a part of the amorphous aluminum can be diffused to the outer surface of the molecular sieve to play a role of enhancing an acid center, the finally prepared hydrocracking catalyst has both high light oil selectivity and high activity, and meanwhile, since the framework structure of the molecular sieve is not changed, high stability can be maintained.

Disclosed is a disinfecting agent for silkworms, whose formulation comprises 16% of chlorine dioxide, 70% of hexadecyldimethyl ethylammonium bromide and water. The disinfecting agent is suitable for sterilizing silkworm rearing house, silkworm tools, silkworm rearing environment and foliage of mulberry leaf during silkworm production.

The invention discloses a processing method for ultrasonic-assisted oyster freezing. The processing method comprises the following steps: shelling, meat taking, rinsing and sterilizing, soaking and preserving, ultrasonic-assisted soaking and freezing, freezing for ice film plating, and cryopreservation. Ozone water is adopted for rinsing sterilization, the microbial membrane structure is destroyed, the ozone water is cleaner than clear water in rinsing, the sterilization effect is achieved, and the ozone water guarantees that the oysters have good quality before being frozen. An ultrasonic technology is combined with dipping freezing, so that the problems of long time, low freezing rate and the like of a traditional freezing method can be effectively solved. The method is assisted by ultrasonic waves for freezing oysters, the diameter of ice crystals formed during freezing is small, damage to muscle tissue of the oysters in the freezing storage process can be reduced, unfreezing loss can be reduced, and freezing denaturation and nutrient loss of oyster protein are reduced.

The invention relates to a xylanase thermosensitive mutant .The sequence of the mutant is shown as SEQ ID No.1 .A preparing method includes the steps that 1, a mutation sequence s02b12 and an expression vector are connected, and a connected product is used for converting escherichia coli to obtain a recombination strain containing s02b12; 2, the recombination strain is cultured, and expression of recombination mutation xylanase is induced; 3, the expressed mutation xylanase S02B12 is recovered and purified; 4, activity of the xylanase S02B12 is measured .The optimal pH value and temperature of the mutation xylanase S02B12 are 5.5 and 50 DEG C respectively, after the xylanse is treated by trypsin and proteinase K at the temperature of 37 DEG C for 1h, enzyme activity is hardly damaged, compared with wild enzymes, thermal properties of the mutation xylanase S02B12 are changed, activity is lower at the temperature of 60 DEG C, inactivation is faster at the temperature of 37 DEG C, and the half-life period at 37 DEG C is shorter than 5 min .The xylanase thermosensitive mutant can be used for low-temperature biotechnology and other fields and particularly used in food industry.

The invention provides a virus-inactivating composition with pH of 3.8 to 5.5, containing (A) 0.02 to 0.3 M arginine and (B) a component such as 0.01 to 10 mM flavonoid, polyphenol, or ascorbic acid derivative, 0.005 to 5 mass % of an arginine derivative, and 0.1 to 2.5 mass % of an extract solution of natural product.

The invention discloses a catalyst for preparing propylene by methyl alcohol and / or dimethyl ether and a preparation method thereof. The catalyst takes HZSM-5 molecular sieve with the crystallite dimension being less than 100 nanometers as raw material, and adopts fluorine-containing compound solution for dipping; after that, the catalyst is prepared by high temperature calcination and has the fluorous mass percentage of 2-20%. The catalyst prepared by the method has very high propylene yield and stability for the reaction for preparing the propylene by transforming the methyl alcohol.

The invention discloses an innocent treatment method of dead livestock, which comprises the following steps: 1) breaking the dead livestock to form a broken material, and hydrolyzing the broken material to form a clinker, 2) carrying out solid-liquid separation on the clinker obtained in Step 1) to form slurry, and 3) carrying out anaerobic digestion on the proceed obtained in Step 2) to achieve the purpose of innocent and resourceful treatment of the dead livestock. The treatment method effectively achieves stabilization, innocuousness and reduction of the dead livestock, thoroughly stabilizes unstable organic substance, produces methane, water and the like, causes no secondary pollution and can be further matched with a biogas slurry and biogas residue treatment system to effectively achieve organic substance reduction of the dead livestock; the methane generated after the anaerobic digestion can serve as high quality clean energy; solid residue after pressing can also be disposed reasonably according to specific circumstances; at the same time, contact between a livestock corpse and a human body and spreading of pathogenic bacteria are avoided; and the method is easy to implement, manage and popularize.

The invention provides a method for extracting and separating ginkgo flavonoids and ginkgolides from ginkgo leaves, comprising three steps: an enzyme inactivation process, an extraction process and a column purification process; the present invention uses alkaline water to cook fresh ginkgo leaves to inactivate enzymes, which can The enzymes in fresh ginkgo leaves are quickly inactivated, and the ginkgo flavonoids in the raw materials can be freed to the greatest extent by soaking in alkaline water. The residual alkaline water after cooking is added to the organic solvent for extraction, which is beneficial to the subsequent extraction. Improve product yield.

The invention discloses a preparation method of whole Chinese yam powder and application of the whole Chinese yam powder, and belongs to the technical field of food processing. According to the preparation method of the whole Chinese yam powder and the application of the whole Chinese yam powder, fresh Chinese yams are taken as a raw material for preparing the whole Chinese yam powder and a beverage thereof. The whole Chinese yam powder is prepared by the following steps of carrying out pretreatment, carrying out color protection, carrying out pulp-grinding, carrying out dehydration, carryingout enzymatic extrusion, carrying out ultra-fine crushing and so on; and the prepared whole Chinese yam powder is used for preparing a Chinese yam pulp beverage. For a series of physical and chemicalchanges occur in the Chinese yam material due to the enzymatic extruding and puffing treatment, the preparation method of the whole Chinese yam powder is capable of effectively transforming or preserving all components in the Chinese yams so as to fully utilize the ingredients, including starch, proteins, fiber and the like, in the Chinese yam raw-material; moreover, addition of stabilizer-type ingredients is also avoided during blending process of the beverage. The Chinese yam pulp beverage prepared by adopting the method disclosed by the invention is high in Chinese yam raw-material utilization rate, good in color, luster and flavor, and high in stability; being stored at room temperature for 6 months, the Chinese yam pulp beverage is free of the phenomenon of precipitate production, browning, stratification and the like.

The invention discloses an atmospheric pressure atomization plasma efficient sterilization device, and a sterilization method. The main body structure of the atmospheric pressure atomization plasma efficient sterilization device comprises a high-voltage electrode, a grounding electrode, medium pipes, a fixed clamping groove, an insulating plate, a supporting plate, a distance adjusting rod, an atomization machine shell, a water inlet, a water inlet switch, an electricity inlet, a separation baffle plate, an ultrasonic atomizer, a mist outlet pipe, an outlet baffle plate and a machine shell cover plate. During an operation, a high-voltage wire and a ground wire are respectively led out from electrode lead-out ports at two ends and then are respectively connected with a high-voltage power supply and the ground, working liquid is introduced, then the power supply is turned on to generate plasma and preheat the device, the ultrasonic oscillator is started to generate water mist, the water mist is discharged through the mist outlet pipe below and enters an activation chamber, and the activated water mist can be directly used for object surface disinfection or space environment disinfection. The technology has the advantages that equipment is simple and portable, cost is low, and the generated atomized plasma is environmentally-friendly, efficient and free from pollution and chemical residues.

The invention discloses a negative oxygen ion sanitizer and a preparation method and application thereof, and belongs to the technical field of air purifying agents. The negative oxygen ion sanitizeris prepared from a negative ion mixed solution, a stable oxygenation mixed solution and a disinfection and sterilization liquid according to a mass ratio of 1: 1: 1, and the negative ion mixed solution is prepared from calcined tourmaline powder, nano cerium oxide and deionized water; the stable oxygenation mixed solution is prepared from anhydrous sodium carbonate, sodium polyacrylate, hydrogen peroxide and deionized water; the disinfection and sterilization liquid is prepared from broadleaf holly leaf extract, Chinese pulsatilla root extract, dandelion extract, chlorophytum comosum extract,lotus leaf extract and deionized water. The invention further discloses a preparation method of the negative oxygen ion sanitizer. The negative oxygen ion sanitizer can release a large amount of negative oxygen ions, and can effectively kill bacteria, fungi, bacillus, escherichia coli, staphylococcus aureus, hepatitis B virus and other germs.

The invention provides a high-efficiency and low-cost proton exchange membrane water electrolysis hydrogen production control method self-consistent with renewable energy sources and a control system thereof, aiming at the characteristics of low iridium or no iridium oxygen evolution catalyst, the input voltage is controlled in the actual operation process of a PEM electrolysis unit with low iridium or no iridium oxygen evolution catalyst; the voltage upper limit is set to enable the input voltage to be lower than the oxidation dissolution potential of the anode low-iridium oxygen evolution catalyst or the iridium-free oxygen evolution catalyst, namely the stable voltage of the low-iridium oxygen evolution catalyst or the iridium-free oxygen evolution catalyst can be ensured, so that the low-iridium oxygen evolution catalyst or the iridium-free oxygen evolution catalyst is prevented from being excessively oxidized and dissolved, and the catalyst is quickly inactivated; therefore, the oxygen evolution catalyst with low iridium content or without iridium element is used for coping with low-fluctuation stable operation power supply response, the cost of an electrolysis system can be effectively reduced by using the oxygen evolution catalyst with high iridium content, and the comprehensive energy conversion efficiency of an electrolysis unit is improved.

The invention provides a heat resistant beta-amylase-trehalose synthase fusion enzyme, an expression gene of the heat resistant beta-amylase-trehalose synthase fusion enzyme, an engineering bacterium secreting the fusion enzyme and application, and belongs to the technical field of genetic engineering. The heat resistant beta-amylase-trehalose synthase fusion enzyme comprises an amino acid sequence with SEQ ID No.1 and another amino acid sequence, and the similarity of the another amino acid sequence to the amino acid sequence with SEQ ID No.1 is 80%-100%. The expression gene of the heat resistant beta-amylase-trehalose synthase fusion enzyme comprises an amino acid sequence with SEQ ID No.2 and another amino acid sequence, and the similarity of the another amino acid sequence to the amino acid sequence with SEQ ID No.2 is 80%-100%. The obtained beta-amylase-trehalose synthase fusion enzyme has a heat resistant property, directly converts amylose into trehalose at the higher temperature, saves the complicated steps of separating and purifying endoenzyme, simplifies the production process of the trehalose, and reduces the production cost.

The invention relates to an activity evaluation method for a Fe-based ft synthesis catalyst, and relates to the field of Fe-based ft synthesis catalysts. The method comprises the following steps that(1) reducing gas is used to reduce and activate the Fe-based ft synthesis catalyst in the oxidation state to obtain the Fe-based ft synthesis catalyst in the reduction activation degree of 80-90%; (2)initial activity of the Fe-based ft synthesis catalyst, obtained in the step (1), in the reduction activation degree of 80-90% is stabilized in the initial active condition; (3) the initial activitystabilized Fe-based ft synthesis catalyst obtained in the step (2) makes contact with H2 and CO synthetic gas to make ft synthesis reaction under the ft synthesis condition lower; and (4) after the ftsynthesis reaction, the carbon formation quantity and inactivation rate of the Fe-based ft synthesis catalyst and selectivity of ft synthesis products are tested. The method can be used to rapidly select the Fe-based ft synthesis catalyst with optimal performance.

The invention discloses a swab sample preserving fluid. The swab sample preserving fluid is prepared from the components of isosorbide dimethyl ether, sucrose fatty acid ester and polyether NPE-108. The preserving liquid is a swab sample preserving liquid which can rapidly inactivate viruses and is good in preservation effect, can preserve viral nucleic acid for 16 days at the normal temperature of 37 DEG C and can preserve viral nucleic acid for 60 days at the temperature of 2-8 DEG C, can effectively protect nucleic acid from being degraded, does not affect subsequent extraction and detection of nucleic acid, and is safe and effective. Moreover, the swab sample preserving liquid can be used for quickly inactivating the preserved virus sample within 10 minutes, so that secondary infection of an operator is effectively prevented.

The present invention relates to industrial sulfur tolerant catalyst for vapor ethylation of coking benzene to prepare ethyl benzene and its preparation process. The catalyst consists of nanometer HZSM-5 zeolite molecular sieve, alpha-Al2O3, and metal or non-metal oxide as modifier. Its preparation process includes high temperature water vapor or ammonia water vapor treatment of 20-200 nm crystal grain size HZSM-5 zeolite molecular sieve catalyst; and soaking the molecular sieve catalyst to support oxide of IIB element zinc, oxide of IIIB element La, oxide of VIB element Microwave oven and / oroxide one non-metal element P for modification. It may be used in the vapor ethylation of coking benzene with sulfur content of 200-800 preparation directly without needing desulfurizing and with the catalyst regenerating period as long as 60 days and other advantages.