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Method for extracting and separating ginkgo flavone and ginkgolide from ginkgo leaves

A technology of ginkgo flavonoids and ginkgolides, which is applied in the field of extracting ginkgo flavonoids and ginkgolides, extracting and separating ginkgo flavonoids and ginkgolides, can solve the problems of low yield and low product content, achieve high content and improve product quality. The effect of simple yield and operation steps

Active Publication Date: 2016-03-23
SHAANXI JIAHE PHYTOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of them choose water extraction and alcohol precipitation or alcohol extraction and water precipitation, and then use macroporous resin for column chromatography. The content of the product obtained by this method is low, and the general yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Add 10 kg of water (containing 200 g of sodium bicarbonate) into a closed container and heat to boil, add 2 kg of fresh ginkgo leaves, and cook for 10 minutes. Take out the leaves, extract once with 90% ethanol at 70°C, filter, and filter the residue once with 80% ethanol at 70°C, filter, combine the extracts, concentrate to 1.1, adjust to neutral, place and filter, and mix the supernatant into 1 / 3 times the amount of polyamide drying. Take another 1.8kg polyamide and 180g alumina to pack the column, use petroleum ether: ethyl acetate = 2:1 to rinse, add the above dried sample to the top of the column, continue to use petroleum ether: ethyl acetate = 2:1 elution Point plate observation, collect the effective component section, after the lactone elution is completed, change the ethyl acetate: ethanol = 8:1 elution, point plate observation, collect the effective component section. The elution sections containing the effective ingredients were respectively concentrated to...

Embodiment 2

[0028] Add 10 kg of water (containing 400 g of ammonia) into a closed container and heat to boil, put in 2 kg of fresh ginkgo leaves, and cook for 5 minutes. Take out the leaves, extract once with 90% ethanol at 70°C, filter, and filter the residue once with 80% ethanol at 70°C, filter, combine the extracts, concentrate to 1.1, adjust to neutral, place and filter, and mix the supernatant into 1 / 3 times the amount of polyamide drying. Take another 2.5kg of polyamide and 0.5kg of alumina into the column, use petroleum ether: ethyl acetate=3:2 to rinse, add the above dried sample to the top of the column, continue to use petroleum ether: ethyl acetate=3:2 to wash After the lactone is eluted, change the ethyl acetate:ethanol=3:1 elution, and spot the plate to observe, and collect the effective component section. The elution sections containing effective ingredients were concentrated to obtain: Product I (Ginkgolide) 3.3g, lactone content 70.9%; Product II (Ginkgo flavonoid) 8.43g...

Embodiment 3

[0030] Add 10 kg of water (containing 400 g of sodium bicarbonate) into a closed container and heat to boil, put in 2 kg of fresh ginkgo leaves, and cook for 15 minutes. Take out the leaves, extract once with 90% ethanol at 70°C, filter, and filter the residue once with 80% ethanol at 70°C, filter, combine the extracts, concentrate to 1.1, adjust to neutral, place and filter, and mix the supernatant into 1 / 3 times the amount of polyamide drying. Take another 1.72kg of polyamide and 0.28kg of alumina into the column, use petroleum ether: ethyl acetate=3:2 to rinse, add the above-mentioned dried sample to the top of the column, continue to use petroleum ether: ethyl acetate=3:2 to wash After the lactone is eluted, change the ethyl acetate:ethanol=4:1 elution, and observe the plate to collect the effective component section. The elution sections containing the active ingredients were respectively concentrated to obtain: product I (ginkgolide) 3.11g, lactone content 75.2%; produc...

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PUM

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Abstract

The invention provides a method for extracting and separating ginkgo flavone and ginkgolide from ginkgo leaves. The method comprises the three steps of an enzyme deactivation process, an extraction process and a column purification process. According to the method, fresh ginkgo leaves are cooked through alkaline water for enzyme deactivation, and enzymes in the raw material of the fresh ginkgo leaves can be rapidly deactivated; by means of alkaline water soaking, the ginkgo flavone in the raw material can be dissociated out to the maximum extent, residual alkaline water after cooking is added to organic solvent for extraction, subsequent complete extraction is facilitated, and the product yield is increased.

Description

Technical field [0001] The invention belongs to the field of extraction and separation of flavonoids and lactones, and relates to a method for extracting ginkgo flavonoids and ginkgolides, in particular to a method for extracting and separating ginkgo flavonoids and ginkgolides from ginkgo leaves. Background technique [0002] Ginkgo is a perennial woody plant, also known as Ginkgo biloba and Gongsun tree. Ginkgo is currently one of the rare gymnosperms in the world. Its survival history can be traced back to 200 million years ago. The tree is more than a thousand years old and is known as the "living fossil". The chemical components of Ginkgo biloba mainly include flavonoids, terpenes, phenols, 25 effective elements and 17 amino acids. [0003] The flavonoid glycosides in Ginkgo biloba are composed of quercetin, kaempferol, isorhamnetin, myricetin, luteolin, and mono-, di- and tri-glucosides of apigenin. The latter three substances account for a large proportion If the content o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61K127/00
CPCA61K36/16A61K2236/10A61K2236/333A61K2236/39A61K2236/55
Inventor 王晓莹肖红郭文华张瑜
Owner SHAANXI JIAHE PHYTOCHEM
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