The invention relates to a natural tea tree oil screen cleaning fluid composed of the following compounds according to parts by weight: the weight ratio among benzalkonium chloride, tea tree oil and ionized water is equal to 9 to 23: 35 to 55: 74 to 98. The cleaning fluid of the invention uses the benzalkonium chloride, the tea tree oil and the ionized water as raw materials, is environment-friendly, can sterilize, is free from damaging the screen coating film, leaves no wiping trace on the screen, can quickly and efficiently remove the dust, dirt and fingerprint on the liquid crystal screen, has the functions of decontamination and sterilization, is anti-static and dustproof, can reduce the accumulated dust, can clear the surface of the screen without trace, can form a protection film on the wiping surface, has sterilizing property, and can avoid bacterial virus spreading so as to help people away from virus. The cleaning fluid of the invention has wide application scope, and can be used for TFT liquid crystal display, notebook computer screen, liquid crystal television, rear projection television, plasma television, VCD and DVD discs, and the like.