Sputum treating liquid for detecting Mycobacterium tuberculosis

A technology for tubercle bacillus and treatment liquid, applied in the direction of microorganism-based methods, microorganism measurement/inspection, microorganisms, etc., can solve the problems of many viscous substances, high cost, poor fluidity, etc.

Inactive Publication Date: 2016-01-27
NANJING BANGBO BIOTECH CO LTD
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AI Technical Summary

Problems solved by technology

The advantage of conventional culture method of Mycobacterium tuberculosis is that it has high sensitivity, specificity, and correct rate. It is a reliable method for identifying dead and live bacteria. It is an irreplaceable method for live bacteria detection and the main monitoring index for chemotherapy response. Too long, it takes 4-8 weeks to report the results, the new rapid culture method, the instrument is expensive and needs to import liquid medium, the cost i

Method used

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Examples

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Embodiment Construction

[0007] Specific examples of the present invention are as follows (preparation of 100ml treatment solution):

[0008] 1. Take 65ml of distilled water, put it into a measuring cup, measure 0.6g of boric acid, 0.1g of disodium EDTA, and 0.1g of SDS respectively, add them into the measuring cup and stir for 10 minutes to fully dissolve.

[0009] 2. Take 10 ml of absolute ethanol, 20 ml of isopropanol, and 5 ml of mucolytic agent, add them into a measuring cup and stir for 5 minutes to mix well.

[0010] 3. Pour the liquids of step 1 and step 2 into the beaker and stir for 10 minutes to mix well. That is, the preparation of the sputum treatment liquid is completed.

[0011] Instructions

[0012] Spit the sputum into the tube containing the sputum treatment solution or take the sputum from the sputum collection tank and add it to the treatment solution, mix well and let the treatment solution liquefy the sample, let it stand for 5 minutes and shake it for 1-3 minutes. Mark the s...

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Abstract

A sputum treating liquid for inspecting standard films of Mycobacterium tuberculosis in sputum comprises ethyl alcohol, isopropyl alcohol, boric acid, disodium EDTA, SDS, a mucus dissolving agent and distilled water and is capable of dissolving phlegm, deactivating pathogenic bacteria such as Mycobacterium tuberculosis as well as viruses in sputum, preventing corrosion and splitting exfoliative cells in sputum. Applied with simple centrifugal operation, the sputum treatment with the sputum treating liquid enables 8 to 10 spits of sputum to be treated at a time, and even sputum left for 24 hours, the treatment quantity of sputum is increased, standard operation is achieved through a standard automatic film-making technique, the operation is simple and easy, detection sensitivity is improved, detection is accurate and reliable, and the sputum treating liquid is suitable for the general investigation of tuberculosis high-risk groups, screening of rural tuberculosis and clinical diagnosis of tuberculosis.

Description

technical field [0001] The invention relates to a sputum treatment solution for detecting Mycobacterium tuberculosis, which is used for the general survey of high-risk groups of pulmonary tuberculosis, the screening of pulmonary tuberculosis in rural areas and the clinical diagnosis of tuberculosis, and belongs to the field of in vitro diagnostic reagents. Background technique [0002] Mycobacterium tuberculosis (M.tuberculosis), commonly known as Mycobacterium tuberculosis, is the pathogenic bacteria that causes tuberculosis. It can invade all organs of the body, but tuberculosis is the most common. Tuberculosis is still an important infectious disease. According to the WHO, about 8 million new cases occur each year and at least 3 million people die from the disease. China is one of the 22 countries with a high incidence of tuberculosis in the world, and 70% of the tuberculosis patients in the Western Pacific region are in China. According to the survey by the Ministry o...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/32
Inventor 郭严顾兵沈翰周玉王玫璇周雷程燕华
Owner NANJING BANGBO BIOTECH CO LTD
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