The invention discloses a preparation method of tauro ursodesoxy
cholic acid. The method comprises the following steps: firstly, dissolving
ursodesoxycholic acid in
organic solvent, reducing the temperature of the
ursodesoxycholic acid to 0 to 10 DEG C, adding amine compounds into the
ursodesoxycholic acid, and stirring the ursodesoxycholic acid; secondly, adding condensing agent and 4-dimethylamino
pyridine after stirring in the first step, and performing stirring reaction at
room temperature, obtaining reaction liquid A; thirdly, adding the reaction liquid A obtained in the second step into
sodium taurocholate
salt water solution, and dropwise adding
triethylamine at
room temperature while stirring so as to ensure that the
triethylamine is reacted with the reaction liquid A; and fourthly, adding
methylene dichloride to perform extraction and liquid separation after the reaction in the third step, then washing a
water layer through
methylene dichloride, adjusting the pH value after washing, performing concentration after stirring, and then obtaining the tauro ursodesoxy
cholic acid. The preparation method has the advantages that the tauro ursodesoxy
cholic acid is prepared through a novel technical process, the
reaction speed is high, the product quality is high, and
raw material is cheap.