128results about How to "High detection cost" patented technology

The invention relates to a green building comprehensive detection device based on Zigbee. The green building comprehensive detection device comprises a support, a data acquisition module, a Zigbee wireless transmission module, a mobile power supply, a transformation module and an upper computer. The data acquisition module, the Zigbee wireless transmission module, the mobile power supply and the transformation module are all installed on the support, the mobile power supply and the transformation module are connected with the data acquisition module and the Zigbee wireless transmission module respectively through a power line, and the data acquisition module is connected with the upper computer through the Zigbee wireless transmission module. Compared with the prior art, the green building comprehensive detection device has the advantages that various detection functions are integrated, various detection data are integrated through a Zigbee wireless data transmission technology, semi-automatic control of a detection instrument is achieved, detection early-stage preparation work is effectively reduced, an assembled structure is adopted, and the green building comprehensive detection device is easy to demount and assemble and convenient to carry.

The invention discloses a pavement asphalt thickness detection method based on vehicle-mounted laser scanning spot cloud, comprising steps of obtaining spot cloud data, performing data rough registration through choosing a fixed object, using a pavement extraction algorithm based on the road shoulder to respectively detect two sets of the pavement points of the point cloud data, using the road shoulder point to perform precise registration on the data in the block, performing plane structure fitting on the pavement inside the grid, and detecting the asphalt thickness. The invention can fast and accurately detect and modify the thickness of the road asphalt, greatly reduces the processing time and the labor cost, effectively guarantees the road reconstruction quality and provides important data support to the civil traffic safety.

An embodiment of the invention discloses energy management equipment, an electronic control system, an electronic control method and an electric vehicle. The energy management equipment comprises an H-bridge unit, a battery pack serving as an input power source of the H-bridge unit and a BMS (battery management system) for performing energy balance control on the battery pack, wherein the electronic control system comprises a master controller, 3N sub-controllers and 3N sets of energy management equipment; the 3N sets of energy management equipment constitute a first face group, a second face group and a third face group; each faces group comprises N sets of energy management equipment, the master controller calculates the PWM (pulse-width modulation) duty cycle of each set of energy management equipment, and the sub-controllers control connection of corresponding energy management equipment according to the PWM duty cycles. The embodiment of the invention solves the technical problems of high battery consistency requirement, high detection cost, battery replacement difficulty and poor balance ability of the BMS due to large-scale series connection of batteries in the battery pack of the conventional electric vehicle, so that dynamic voltage equalization after large-scale series connection of the battery pack is realized.

The invention relates to the technical field of digital image processing, in particular to a camera shielding detection method. The camera shielding detection method includes the steps of 1, building a camera resource configuration module; 2, enabling a monitoring center to judge whether a camera is in operation or not according to operation state information, and to acquire a current-frame digital image from the camera in the operation state; 3, performing image preprocessing; 4, building an image brightness background, smoothing the image to a smooth brightness distribution diagram by an average filtering method, and extracting possibly shielded sectors Si of the image by a dynamic threshold method; 5, subjecting the possibly shielded sectors Si to connected component analysis, setting a sector threshold value ST, selecting the possibly shielded sectors Si not less than the sector threshold value ST, and defining the selected possibly shielded sectors Si as high-probability shielded sectors S2; 6, computing shielded probability P of the high-probability shielded sectors S2; 7, judging the relation between the shielded probability P and preset shielded probability PT, if P>PT, judging the high-probability shielded sectors as final shielded sectors, and if not, judging the high-probability shielded sectors as unshielded sectors. The camera shielding detection method is high in detection efficiency.

The invention discloses a digital imaging acquisition system for aggregate grading detection. The system consists of an aggregate sampling device, an image acquiring device, an optical processing optimizer and communication and storage equipment, wherein the aggregate sampling device comprises a material dividing plate, a distance adjusting rod and an angle adjusting rod; the image acquiring device comprises an infrared laser, a linear array camera and an image acquiring card; the optical processing optimizer comprises a filter device and a diffuse coating layer; and the communication and storage equipment comprises a data line and a computer. A digital imaging acquisition method for aggregate grading detection comprises the following steps of: setting the aggregate sampling device; setting the image acquiring device; setting the communication and storage equipment; acquiring an image of a sampled aggregate; and transmitting and storing the image. An aggregate stream is sampled and acquired by arranging the material dividing plate, so the problem that the image of a falling stream of the aggregate must be completely acquired is solved and the defects of poor quality of the acquired image, difficult separation of particles and a large amount of processing data are overcome.

The invention relates to a tetracyclines aptamer, an aptamer electrochemical biological sensor for detection of tetracyclines, and a preparation method and a detection method of the sensor. The tetracyclines aptamer is a single strand DNA probe containing a TTGAGCCCT sequence and having the length of 13-20 bp, and an amino is modified by a 5' end, namely 5'-NH2-(CH2)6-. The aptamer electrochemical biological sensor for the detection of the tetracyclines contains the tetracyclines aptamer. The sensor improves the sensitivity of the detection of the tetracyclines, and can detect the tetracyclines in a minimum concentration of 1.0 ng / mL; and the sensor greatly reduces the cost of the detection of the tetracyclines, has simple and convenient operation, and can rapidly detecting the tetracyclines.

Provided is a coherent anti-stokes Raman microscopy imaging system generated on the basis of double-soliton pulses. The microscopy imaging system comprises an optical fiber laser for generating original femtosecond pulses, a double-soliton pulse generation and spectrum expansion unit for generating the double-soliton pulses, an optical pulse scanning unit for achieving pulse time-domain scanning, a power ratio control unit for adjusting the ratio of the double-soliton pulses entering the double-soliton pulse generation and spectrum expansion unit and the optical pulse scanning unit, a microscopy imaging system for conducting focusing excitation and signal photon collection on a sample to be detected and a part for conducting detection, extraction process and microscopic image display on signal photons. According to the coherent anti-stokes Raman microscopy imaging system, the double-soliton pulses which have the time domain capable of being distinguished and have frequency differences are obtained on the basis of a full optical fiber system, an excitation light source system is simplified, frequency modulation resonance excitation is achieved, the influence of non-resonant signals on a coherent anti-stokes spectrum is eliminated, and the contrast ratio of coherent Raman microscopy images is significantly increased.

The invention relates to a ractopamine aptamer, an electrochemical biosensor for detecting the ractopamine aptamer same, a method for manufacturing the electrochemical biosensor and a method for detecting the ractopamine aptamer by using the electrochemical biosensor. The ractopamine aptamer is a single-chain DNA (deoxyribonucleic acid) probe which contains an AGTGCGGGC sequence and has a length of 13-20bp, and the amino group of 5'-NH2-(CH2)6- is modified on the 5' terminal. The electrochemical biosensor for detecting the ractopamine aptamer comprises the ractopamine aptamer, has higher ractopamine detection sensitivity, can be used for detecting 0.1ng / mL of ractopamine at least, ensures that the ractopamine detection cost is lowered greatly, is easy and convenient to operate and can realize the rapid detection of the ractopamine.

The invention provides an aptamer-modified nanogold colorimetric detection-based analysis method of ibuprofen. The aptamer-modified nanogold colorimetric detection-based analysis method comprises following steps: single strand nucleic acid ibuprofen aptamer is taken as an identification probe of ibuprofen, ibuprofen standard solutions with different concentrations are mixed with gold nanoparticle solutions, single strand nucleic acid ibuprofen aptamer solutions, and salt solutions so as to obtain standard detection solutions of different concentrations; after reaction, an ultraviolet spectrophotometer is adopted to detect light absorption values of obtained systems based on different discoloration degrees of the systems caused by gold nanoparticle coagulation, a standard curve is drawn, and the concentration of ibuprofen of a sample to be detected is determined according to the standard curve. According to the aptamer-modified nanogold colorimetric detection-based analysis method, detection can be finished in a short time, and qualitative detection and quantitative determination results can be obtained; and the aptamer-modified nanogold colorimetric detection-based analysis method is high in specificity and stability, wide in application range, and low in cost, and is convenient for popularization and routine application.

An ultrasonic probe performs flaw detection of a high (t / D) metal pipe. The front end portion of a transducer has at least a portion with an asymmetrically curved shape having a radius of curvature which progressively increases from one end towards the other end of the front end portion. The probe is disposed with respect to the pipe with its end having the smaller radius of curvature positioned on the side away from the direction of propagation of refracted waves, the end having a larger radius of curvature thereof positioned on the side in the direction of propagation of refracted waves. Angle beam flaw detection is carried out such that incident waves are generated which generate refracted longitudinal waves which do not reach the inner surface of the metal pipe and refracted transverse waves which are focused on the inner surface of the pipe.

The invention provides a mass spectrum system used for detecting biomolecules. The system comprises a mass spectrometer data collecting system, an intelligent fuzzy expert system, a knowledge base collecting system and a model collecting system. The mass spectrometer data collecting system is used for detecting mass spectrum data of a sample; the intelligent fuzzy expert system can carry out intelligent analysis on the mass spectrometer data; the knowledge base collecting system can store and manage related knowledge of mass spectrum detection in advance through a cloud center; the model collecting system is used for sending the known mass spectrum model data to a knowledge management system and a mass spectrum model storage device according to instructions. The system is supported by cloud center calculation, intelligent learning and storage are performed through the knowledge base collecting system, the intelligent analysis is carried out on the mass spectrum data through the intelligent fuzzy expert system, and simulation with lower cost is carried out to generate a large amount of training data; the system is conductive to analyzing large samples, is suitable for nonprofessional operations, achieves monitoring personal health information in real time, and meets personalized medical service requirements.

The invention relates to a full-cycle or half-cycle synchronous frequency measurement corrected digital demodulation detection system and detection method of amplitude-modulated signals. The full-cycle or half-cycle synchronous frequency measurement corrected digital demodulation detection system and method can be adopted to realize the amplitude detection of the amplitude-modulated signals. According to the detection system, a micro controller unit is adopted to control a high-speed analog-to-digital conversion unit to perform signal acquisition, and lock-in amplified digital integral and filtering can be realized through absolute value cumulative averaging calculation; and the starting point of a full cycle or a half cycle is captured, and the frequency of the signals is measured through using high-frequency counting, and frequency correction is performed on a detection result. Compared with an existing detection system using a lock-in amplifying circuit, the detection system of the invention has the advantages of simple circuits, no middle debugging process and high detecting precision; compared with an existing simplified digital demodulation detection system, the detection system of the invention can assist in decreasing system detection noise and improving detection accuracy under the premise that the complexity of the circuits and algorithms as well as detection time and cost are not increased.

The invention provides a testing method for total phosphorus content in soil based on image analysis, and the testing method for the total phosphorus content in soil based on the image analysis relates to the soil chemical analysis. The testing method for the total phosphorus content in the soil based on the image analysis includes the following steps of pretreating soil samples by a method of melting sodium hydroxide, preparing samples, colorating, and acquiring a concentration value c. The step of acquiring the concentration value c includes digital imaging after colorating and image analysis software is used for acquiring red, green, blue (RGB) values of the soil samples. A gray scale value Gr is acquired according to the RGB values, and the concentration value c is acquired according to the grey value Gr. The testing method for the total phosphorus content in the soil based on the image analysis has the advantages of being convenient and fast to test, easy to operate, low in testing cost, and suitable for on-site analysis.

The invention discloses a lectin chip for joint detection of ten carbohydrate chains in human serum as well as preparation and use methods of the lectin chip. Ten specific lectin probes are fixed on the surface of a solid-phase carrier in a dot matrix manner, and joint detection of ten carbohydrate chains in human serum is realized by coating ten specific lectins, wherein the solid phase carrier is a gold foil chip chemically modified by 16-amino-1-hexadecanethiol and DOTA-NHS-ester, and three carboxyls at the tail end of DOTA-NHS-ester are activated by EDC and NHS to fix specific lectins as probes. The chip has the advantages of high throughput, high selectivity, high specificity and the like and is more applicable to research of human tumor glycosyl spectrum characteristics.

The invention provides a method for ultra-sensitively detecting small molecular substances, and belongs to the field of biotechnology. To-be-detected small molecule substances in the method are one ormore than two of antigens, ligands, biotins, substrates and polysaccharides. The method comprises the following steps: respectively marking solid phase carriers by using various to-be-detected smallmolecules; respectively marking substances for recognizing the various to-be-detected small molecules through specific action by using all fluorescence probes to obtain fluorescent markers; adding thesolid phase carriers marked by all the to-be-detected small molecules and all the fluorescent markers into a sample solution for reaction; after the reaction is ended, separating the solid phase carriers and releasing the fluorescent probes combined to the surfaces of the solid phase carriers; detecting the fluorescent probes with a single molecular detection method, and calculating the concentrations of all the to-be-detected small molecules in the sample solution according to a standard working curve. By adopting the method for ultra-sensitively detecting the small molecular substances provided by the invention, various small molecular substances can be rapidly and ultra-sensitively detected.

The invention discloses benzothiazole-triphenylamine dye with an AIE effect and a preparation method and application thereof. The preparation method of the benzothiazole-triphenylamine dye comprises the steps that benzothiazole-2-acetonitrile and diphenyl amino-4-benzaldehyde are dissolved into absolute ethyl alcohol, ammonium acetate is added, and reacting under stirring is conducted at room temperature; a coarse product is obtained through filtering, and the pure benzothiazole-triphenylamine dye is obtained through recrystallization. According to the benzothiazole-triphenylamine dye, a cyanide ion detection method which is high in selectivity and sensitivity, is not disturbed by other negative ions, can achieve the detection limit as low as the ppm level and is low in detection cost and convenient and fast to operate can be achieved through an aqueous solution, and the problems that in the cyanide ion detection field, the cost is high, operation is complex, and detection is insensitive can be solved.

The invention discloses a braking controlling method for the point-to-point position servo of the induction machine. The energy consumption braking servo phase trajectory of the induction machine runs commonly along the optimum switched-line, which is the time price suboptimum braking controlling method, the braking controlling method is that: the induction machine runs at the predetermined maximum speed according to the error amount deltaX of the initial displacement coordinate X2 and the target displacement coordinate X of the controlled moving object, when the induction machine reach the displacement coordinate X1 of control amount state switch shifting, the Bang-Bang controller in the braking controlling system sends out a braking signal, the power inverter outputs a variable stator braking current with the amount in the certain function relation with the rotor rotating speed omega[r], so that the energy consumption braking phase trajectory of the induction machine runs along the optimum switched-line, accordingly the time price of the target displacement coordinate X is optimal, and the variable current braking energy consumption is less, thus having theory innovation and practical value.

The invention relates to a balance lever machine. The balance lever machine comprises a reverse rack, a stand arranged on the reverse rack, a girder traversing the stand and a mobile bracket arranged between the stand and the girder, wherein a first screw rod is arranged in the center of the stand; the center at the upper end of the mobile bracket is provided with a first nut matched with the first screw rod; the girder comprises a body and actuating arms which are symmetrically arranged at both ends of the body; the end part of each actuating arm is provided with a balance tool rest; and a balance device is fixedly arranged in the girder and comprises a support cutter positioned in the center of the girder, a balance cross-shaped cutter positioned on one side of the support cutter and balance cutters positioned at the front and rear ends of the girder. The balance lever machine can serve as a balance and used for detecting the performance of workpieces, and is wide in application range and high in detection precision.

The invention discloses a multiple PCR detecting method for pathogen of sugarcane mosaic disease and ratoon stunting disease, which is characterized in that (1), a sheathing leaf at the stem base portion of a sugarcane plant is employed as detecting sample. (2), reverse transcription primer and primer pair of PCR detection cpc1 and cpc2 and RSD primer pair F1 and F2 are designed, sugarcane mosaic virus and genetic fragment of pathogen of sugarcane ratoon stunting disease are amplified. (3), total nucleic acid is extracted, and the RNA thereof is turned into reverse transcription via cDNA. (4), reaction system and amplification program of the one-step method with multiple RT-PCR are designed, and the nucleic acid is amplified. (5), gel electrophoresis analysis and amplification results and specificity are employed, and simultaneously decuple progressive dilution is used to detect the sensibility of the one-step method with multiple RT-PCR. The detecting method provided by the invention accomplishes reverse transcription and PCR reaction and simultaneously detects pathogen of two diseases by extracting the total nucleic acid of sugarcane, which has the advantages of simple operation, economy of time, low cost, high specificity and sensibility and the like.

The invention provides a testing method for coaxiality of flanges at two ends of a pipeline valve. The method comprises the steps of symmetrically arranging a first flange and a second flange at two ends of the pipeline valve, and placing the first flange and the second flange on a testing platform; determining whether a bolt hole exists at a maximal dimension position of the first flange; if yes, measuring the height of the bolt hole on the first flange and the height of the bolt hole on the second flange, and obtaining a height difference of each pair of symmetrical bolt holes on the positions of the first flange and the second flange; if otherwise, measuring the height of the bolt hole at a horizontal position which is nearest to the maximal dimension position on the first flange and the height of the bolt hole that corresponds with the second flange, thereby obtaining a height difference of each pair of symmetrical bolt holes on the first flange and the second flange; determining whether the height difference is in a preset standard; if yes, determining qualification of the coaxility between the first flange and the second flange; and otherwise, determining unqualification of the coaxility between the first flange and the second flange. The testing method can test all pipeline valves with different dimensions and furthermore has advantages of low requirement for a testing environment, high testing speed and low cost.

The invention provides a tumor imaging system based on near-infrared spectrum blood oxygen concentration measurement and an experiment detection method of the system. The problems that traditional tumor detection equipment is heavy, expensive and high in diagnosis cost are mainly solved. The system comprises a main control part, and the main control part comprises a control kernel STM32F103 which is respectively connected with a light signal generation module, a data storage and communication interface module, and a signal conditioning and analog-digital conversion module, wherein the data storage and communication interface module comprises an SD card, a USB communicating with an upper computer, and a serial interface; the signal conditioning and analog-digital conversion module comprises a data amplifying and filtering part and an analog-digital conversion part and is responsible for signal collection and preprocessing. A probe part comprises the light signal generation module and a light signal receiving module. The system has the advantages of being free of radiation, low in manufacturing cost, portable, easy to operate, high in recognition rate, capable of achieving imaging and the like.

The invention discloses naphthoquinone compound alkannin, which can be used for quantitatively testing the enzyme activity of glucuronic acid transferase UGT1A9 and UGT1A8 as UGT1A9 and UGT1A8 specific probe substrate. The use is implemented by the following step of: selecting alkannin as a specific probe substrate, carrying out the UGT catalytic reaction of a specific substrate by using a UGT in-vitro incubation system, quantitatively testing the enzyme activity of UGT1A9 and UGT1A8 in biological samples and cells by quantitatively testing the lost amount of the substrate or the generated amount of the product. The substrate can be used for quantitative evaluation of the enzyme activity of UGT1A9 / 1A8 in different species, individuals and biological samples, calibration of propofol anesthetic metabolizing capacity of certain tissues, and calibration and estimation of the elimination rate of compounds which are mainly metabolized by UGT1A9 in specific organisms based on in-vitro metabolism dynamic data.

The invention discloses a detection material resisting NMDAR autoantibodies in human body fluid and a preparation method and application thereof. According to the invention, NMDAR antigens are arranged on a carrier membrane through coating to prepare an anti-NMDAR-receptor detection material, specific NMDAR antibodies in human serum and cerebrospinal fluid can be combined with the antigens, alkaline phosphatase substrate-ligand reaction is utilized for color development, the sealing material is added in the color development reaction, and whether a detected sample contains the NMDAR antibodiesor not can be directly judged through naked eye observation. The method has the advantages of high sensitivity, easy and convenient operation and rapid detection, and is conducive to recognition anddiagnosis of anti-NMDAR-receptor encephalitis.

The invention discloses a method, primer, probe and kit for detecting six unusual fusion types of BCR-ABL fusion genes such as e6a2, e8a2, e19a2, e1a3, e13a3 and e14a3. The method comprises the following steps: performing primary screening on blood samples by adopting two tubes of fluorescent quantitative polymerase chain reaction (PCR), and quantitatively indentifying specific types of the blood samples belonging to the a2 or a3 group through primary screening judgment. Therefore, the screening cost is reduced, and the detection throughput is also improved. According to the detection method, primer, probe and kit, high sensitivity, high specificity and high detection throughput can be realized, and the method is a rapid and accurate type screening and identifying method.

The invention discloses a lead screw bearing block alignment method. A dial indicator frame is arranged at the position, close to a screw nut base, on a workbench, a machine tool is started, and the workbench records the read number of a pointer on the screw nut base; adjustment is conducted according to the record, the dial indicator frame is adsorbed to a lathe bed, and the pointer of a dial indicator makes contact with the surface of a nut base or the screw nut base to be adjusted; a fastening screw at the to-be-adjusted part is loosened, and the fastening screw is moved through a jack or a crowbar; when the deflection distance and the earlier-stage measuring value are the same, the fastening screw at the part is fastened; adjustment is conducted in the vertical direction, the fastening screw at the to-be-adjusted part is loosened according to the earlier-stage measuring result, and a gasket is additionally arranged to the position below the fastening screw and fastened; and the machine tool is started for rechecking. According to the lead screw bearing block alignment method, few earlier-stage preparing tools are adopted, and detection can be conducted only by preparing the ordinary dial indicator and the dial indicator frame; no requirement exists for the distance between a guide rail and a lead screw; influences from lead screw deflection are weak, and the state of the detection process and the state in the actual machine tool usage process are the same; and the influences on the measuring range from the screw nut base are weak.

The invention discloses a quick testing tool for a machining dimension of a complicated casting and a testing method thereof. The quick testing tool comprises a testing platform, a reference positioning assembly, a lateral positioning assembly, a pin assembly and a testing measuring tool. A lower end surface testing boss is arranged on the testing platform and furthermore the contour of the lower end surface testing boss matches the contour of a lower end-surface bevel of a standard-dimension casting, wherein a clearance exists between the contour of the lower end surface testing boss and the contour of the lower end-surface bevel of the standard-dimension casting. An upper end surface detecting template is fixed by means of the pin assembly. Furthermore the contour of the upper end surface detecting template matches the contour of the upper end-surface groove of the standard-dimension casting. Testing to the edge bevels of the upper end surface and the lower end surface of the casting can be finished by means of a sliding condition of the testing measuring tool in the clearance. The edge contour of a lateral testing template matches the contour of edge bevels at two sides of the standard-dimension casting, and furthermore the edge contour of the lateral testing template abuts against the contour of the edge bevels at two sides of the standard-dimension casting. Testing to the edge bevels at two sides of the casting can be finished by means of a sliding condition of a bevel contour template at the edge bevels at two sides of the casting. The quick testing tool and the testing method have advantages of simple structure, convenient operation, high use effect, high use value and high popularization value.

The invention discloses a method for detecting ferric ions through triphenylamine dye. The method comprises steps as follows: (1), the triphenylamine dye is dissolved in a hydrophile organic solvent, a triphenylamine dye stock solution is prepared and diluted with water, and a triphenylamine dye containing water solution is prepared; (2), a to-be-detected sample is added dropwise, whether fluorescence of the water solution is quenched or not is observed under excitation of light with a wavelength of 365 nm, if the fluorescence of the solution is quenched, the sample contains the ferric ions, cyanide ions or sulfite ions, nuclear magnetic resonance hydrogen spectroscopy is used for aiding in distinguishing, and whether the sample contains the ferric ions can be determined. The method has high selectivity and sensitivity and is basically not interfered by other cations; the detection limit is low and can reach a ppb level; response is quick and is made in 1 min; the method has the advantages of low detection cost, simple operation and the like.

The invention discloses a method for measuring the content of furfural in crude aldehyde. The content of furfural in crude aldehyde is calculated by drawing a refractive index-furfural content standard curve. Because the indicator of refractive index is specified in the technical indicator of industrial furfural, and furfural manufacturers have refractive index testers, so the furfural manufacturers do not need to purchase additional expensive analytical instruments by adopting the method disclosed by the invention, thus reducing the detection cost, meanwhile, the detection method disclosed bythe invention is short in test time, and thus can be applied to the rapid detection of the content of furfural in crude aldehyde.

The invention discloses a detection material for an anti-myelin oligodendrocyte glycoprotein (MOG) autoantibody in human body fluid, a preparation method and application. An MOG antigen coats an NC membrane to prepare the anti-MOG receptor detection material, specific MOG antibodies in human serum and cerebrospinal fluid are combined with the antigen, color development is conducted through an alkaline phosphatase substrate-ligand reaction, a sealing material is added in a color development reaction, and whether a detection sample contains the MOG antibodies or not can be directly judged through visual inspection. The method has the advantages of high sensitivity, easy and convenient operation, quick detection and the like, and is conducive to identification and diagnosis of anti-MOG receptor encephalitis.

The invention discloses an immunofluorescence kit for quantitatively detecting the content of troponin I and a preparation method. The preparation method comprises the following steps: preparing a troponin antibody-large-particle fluorescent particle conjugate; preparing a nitrocellulose membrane labeled antibody; assembling a reagent card; preparing a TNI calibration product; establishing a standard curve; calculating the content of the troponin in a sample to be detected. The immunofluorescence kit for quantitatively detecting the content of the troponin I comprises a nitrocellulose membrane, a reagent and the calibration product. The lowest detection limit of the kit can reach 0.1ng / ml and the requirement of clinical application is met; the fastest time of a process from sample acquisition to detection result presentation only needs 10 to 15 minutes so that more expensive time for patients is provided, and illness states of the patients can be dynamically detected. The immunofluorescence kit for quantitatively detecting the content of the troponin I has good result relevance, no remarkable difference and an accurate and reliable detection result, and can be used for clinically replacing imported products; the detection cost is greatly reduced.