Aptamer-modified nanogold colorimetric detection-based analysis method of ibuprofen

Abstract

The invention provides an aptamer-modified nanogold colorimetric detection-based analysis method of ibuprofen. The aptamer-modified nanogold colorimetric detection-based analysis method comprises following steps: single strand nucleic acid ibuprofen aptamer is taken as an identification probe of ibuprofen, ibuprofen standard solutions with different concentrations are mixed with gold nanoparticle solutions, single strand nucleic acid ibuprofen aptamer solutions, and salt solutions so as to obtain standard detection solutions of different concentrations; after reaction, an ultraviolet spectrophotometer is adopted to detect light absorption values of obtained systems based on different discoloration degrees of the systems caused by gold nanoparticle coagulation, a standard curve is drawn, and the concentration of ibuprofen of a sample to be detected is determined according to the standard curve. According to the aptamer-modified nanogold colorimetric detection-based analysis method, detection can be finished in a short time, and qualitative detection and quantitative determination results can be obtained; and the aptamer-modified nanogold colorimetric detection-based analysis method is high in specificity and stability, wide in application range, and low in cost, and is convenient for popularization and routine application.

Description

technical field [0001] The invention relates to the technical fields of colorimetric analysis and environmental detection, in particular to an aptamer biosensor for detecting ibuprofen, and a preparation and detection method of the sensor. Background technique [0002] In recent years, people have begun to pay attention to the impact of trace pollutants such as pharmaceuticals and personal care products (PPCPs) on human health and the ecological environment, and have developed into a hot issue around the world. Pharmaceuticals and personal care products (PPCPs) include various prescription and non-prescription drugs (Chinese medicine, steroid hormones, anti-inflammatory drugs, antibiotics, analgesics, tranquilizers, antihypertensive drugs, etc.), personal care and skin care cosmetics, fragrances, Health care products, hair dyes, diagnostic agents, disinfectants, cleaning agents and other components added in manufacturing such as preservatives, etc., cover a wide range. At p...

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Problems solved by technology

[0004] The purpose of the present invention is to provide an electrochemical analysis method based on the ibuprofen aptasensor for the shortcomings of the existing technology for detecting ibuprofen such as low sensitivity and complicated operation. The single-stranded nucleic acid aptamer probe of ibuprofen combines with gold nanoparticles to form a gold nanoparticle-nucleic acid aptamer composite probe. In the presence of ibuprofen, the nucleic acid aptamer probe and ibuprofen Specific binding, which causes the conformational change of the nucleic acid aptamer on the surface of the gold nanoparticles, reduces the tolerance of the gold nanoparticles to salt, and the blue phenomenon of the aggregation of the gold nanoparticles appears, and realizes the ultra-sensitive and fast ibuprofen detection

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