A Fluorescent Detection Method Using Nucleic Aptamers and Magnetic Materials to Detect Thrombin

A nucleic acid aptamer and fluorescence detection technology, applied in the field of biochemistry, can solve the problems of complex assembly and low sensitivity, and achieve the effect of improving dispersion, high sensitivity and high affinity

Active Publication Date: 2022-01-18
TIANJIN UNIV OF SCI & TECH
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Problems solved by technology

Although the application of aptamers in optics and electrochemistry has a good linear range for target molecules, their detection has disadvantages such as low sensitivity and the need for complex assembly.

Method used

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  • A Fluorescent Detection Method Using Nucleic Aptamers and Magnetic Materials to Detect Thrombin
  • A Fluorescent Detection Method Using Nucleic Aptamers and Magnetic Materials to Detect Thrombin
  • A Fluorescent Detection Method Using Nucleic Aptamers and Magnetic Materials to Detect Thrombin

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Embodiment Construction

[0052] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0053] In recent years, nanoparticles have broadened the ways in which problems in chemistry, biology, and medicine can be solved. Among them, ferric oxide (Fe 3 o 4 ) Magnetic Nanoparticles (Magnetic Nanoparticles) is one of the promising new magnetic materials developed after the 1970s. Because of its special chemical, physical and physiological properties such as good biocompatibility, superparamagnetism and easy surface functionalization, it is widely used in nuclear magnetic resonance imaging, drug and gene delivery, cell labeling, biological separation, biological Catalysis, adsorption of pollutants and biosensors.

[0054] Fe 3 o 4 The application of nanoparticles in biosensors prompted us to do further research, in which we foun...

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Abstract

The present invention relates to a kind of fluorescence detection method that utilizes nucleic acid aptamer and magnetic material to detect thrombin (thrombin), provides a kind of based on nucleic acid aptamer and PEI-Fe 3 o 4 Novel biosensor for detection of thrombin concentration in which oligonucleotides are negatively charged, PEI‑Fe 3 o 4 Amino-rich and positively charged, fluorescently labeled aptamers react with PEI‑Fe in buffer 3 o 4 Adsorbed to Fe due to electrostatic attraction 3 o 4 surface to quench the fluorescence. In aptamer / PEI‑Fe 3 o 4 Thrombin is added to the complex system, and thrombin binds to the aptamer to keep it away from PEI‑Fe 3 o 4 surface, the fluorescence is restored. Therefore, the concentration of thrombin can be detected through the change of the fluorescence value. This discovery only needs to change the sequence of the nucleic acid aptamer to realize the detection of other substrate molecules, which has great research and application in the field of thrombin detection. significance.

Description

technical field [0001] The invention belongs to the field of biochemistry and relates to nanotechnology, in particular to a fluorescent detection method for detecting thrombin by using nucleic acid aptamers and magnetic materials. Background technique [0002] Thrombin is a multifunctional serine protease that plays an important role in physiological and pathological processes such as blood coagulation, inflammation, anemia, and wound healing, and its content is an important indicator of coagulation mechanism. At the same time, thrombin can also be used as a biomarker for the diagnosis of related diseases. Therefore, the establishment of a simple, rapid, and highly sensitive method for detecting thrombin is of great significance to the early diagnosis of clinical diseases, the development of disease courses, and the monitoring and evaluation of curative effects. [0003] Traditional methods for detecting thrombin include high performance liquid chromatography, biological ma...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/56G01N21/64
CPCG01N21/6428C12Q1/56
Inventor 马龙刁爱坡满淑丽孙娜娜
Owner TIANJIN UNIV OF SCI & TECH
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