39results about How to "Efficient marking" patented technology

The invention discloses a gantry type hull sectional drawing machine and belongs to the technical field of shipbuilding and oceanography engineering. The hull sectional drawing machine is composed of a drawing mechanism and a gantry. The drawing machine mounted on the gantry comprises a trolley and a multi-freedom-degree drawing arm; and the gantry is provided with a travelling mechanism on rails. The gantry supports the trolley and the drawing arm; the drawing mechanism which provides high-accuracy multi-freedom-degree movement and locating is a fundamental structure of the drawing machine and is composed of a plumbing arm, swing arms, omni-directional mechanisms, Z-shaped knuckle, a telescopic arm, a pen clamping frame and the like. The whole machine design is ingenious, the structure is concise, the mounting and dismounting is convenient, the hardness and the stiffness are high, the maintenance is simple, and the reliability is high. Automatic, efficient and high-accuracy sectional line drawing can be performed on steel structures, and the drawing machine is an essential manufacture device in modern shipbuilding enterprises and has great significance for improvement of manufacturing technology levels, product quality and economic benefits of shipbuilding and oceanography engineering equipment.

The invention discloses a vector for efficiently labeling zebra fish PGC, and a preparation method and a use of transgenic fish. A Gal4 / UAS transcription activation system is utilized and mRFP is used as a report gene to realize an inductive gene expression regulation technology in the primordial germ cells of zebra fish. The preparation method of the transgenic fish comprises the following steps: 1, respectively constructing activation transgenic line Tg (kop:KalTA4) and an effect transgenic line Tg (UAS:mRFP); and 2, hybridizing Tg (kop:KalTA4) male fish with Tg (UAS:mRFP) female fish to obtain the transgenic fish for efficiently labeling the primordial germ cells of zebra fish. The transgenic fish for efficiently labeling the primordial germ cells of zebra fish can be widely applied to the fish bioengineering.

The invention provides a stamping device for an autoclaved aerated concrete building block. The stamping device comprises a lifting platform and a conveyer belt device, wherein the lifting platform isused for lifting the autoclaved aerated concrete building block to the conveying front end of the conveyer belt device, and the conveyer belt device is used for conveying the autoclaved aerated concrete building block to a stamping area for stamp mark; the top of the lifting platform is transversely provided with a first hydraulic telescopic rod, and the telescopic end of the first hydraulic telescopic rod is vertically connected with a first push plate; a photoelectric sensor is arranged at the conveying front end of the conveyer belt device, and the conveying rear end of the conveyer belt device is provided with a stamping device body used for carrying out stamp mark on the autoclaved aerated concrete building block; and the photoelectric sensor is electrically connected with the inputend of a controller, the output end of the controller is electrically connected with a frequency converter, and the frequency converter is electrically connected with the input end of a driving motorof the conveyer belt device. The stamping device is high in automatic operation and efficiency, capable of replacing manual stamping, and stable, accurate and efficient in stamping.

The invention discloses an event extraction and processing method for case-following electronic files. The method comprises the following steps: step 1, acquiring required file data from a case-following electronic file circulation processing platform and storing the required file data into a database; 2, constructing an event trigger word dictionary, matching an electronic file event descriptionparagraph, and performing text preprocessing methods such as sentence segmentation, word segmentation and part-of-speech tagging; 3, event attribute extraction: combining dependency syntax analysis and a semantic role labeling method to obtain six event attributes including an applicant, a susceptor, a behavior, time, a place and a mode of an event; and step 4, event aggregation: aggregating the atomic events into topic events, combining similar topic events, and storing the topic events into an event database. According to the method, the problem that crime fact information is rapidly obtained by the case-following electronic file is solved, event extraction and organization can be more accurately carried out on crime facts, and the method is a basis for efficient and high-quality paper marking.

The invention discloses a sterol-based hapten-alkaline phosphatase cross-linked product and a preparation method thereof. According to the present invention, labeling is performed with the carboxyl contained in modified sterol or a sterol-based molecule derivative, and the sterol or the sterol-based molecule derivative is labeled on alkaline phosphatase molecules by using carbodiimide and N-hydroxysuccinimide; the efficiency of the method for preparing the alkaline phosphatase-labeled sterol-based hapten is high; the activities of various components of the cross-linked product are good; the cross-linked product has high sensitivity; the method has characteristics of simple operation and short preparation period; and the sterol-based hapten-alkaline phosphatase cross-linked product is provided.

The invention discloses a method for integrally marking drosophila proteomes by using stable isotope labeling with amino acids in cell culture (SILAC) and a special culture medium. The special culture medium for marking drosophila by using the SILAC is prepared according to the following method: a saccharomycete body marked by using the SILAC is coated on a drosophila culture medium to obtain the special culture medium for marking the drosophila by using the SILAC. An experiment proves that the culture medium for marking the drosophila by using the SILAC can be specially used for marking the drosophila proteomes by using the SILAC, can achieve rapid and high-efficient marking, and creates conditions for preparation of quantified interior labels and high-precision quantified proteome studying.

The invention discloses a method for identifying amino metabolite-containing isomeride by direct mass spectrometry, which comprises the following steps: amino metabolite derivatization reaction: preparing an amino metabolite standard solution from amino metabolite, and adding a derivatization reagent solution to perform derivatization reaction to obtain an amino metabolite derivatization product;amino metabolite derivatization product detection: adding a solvent into the prepared amino metabolite derivatization product to prepare a heavy suspension, diluting the obtained heavy suspension, andcarrying out mass spectrometry sample introduction detection; mass spectrometry conditions are as follows: a positive ion mode is adopted, and an ion source is ESI; MS and MS / MS scanning is automatically carried out according to a data mode during operation of the mass spectrometer, and 10-50 precursor ions with the highest abundance are obtained through HCD fragmentation separation for analysisand identification. According to the method, the N-diisopropyl phosphate-L-alanine-N-hydroxysuccinimide ester is applied to identification of the isomeride for the first time, and the method for identifying the amino metabolite isomeride has high accuracy and wide applicability.

The invention belongs to the technical field of fastener production, and particularly relates to a marking device for fastener production. The marking device aims to solve the problems that an existing marking mode needs to hold a fastener by hand and send the fastener to a marking head for marking, the efficiency is low, and an operator is prone to being hurt. The marking device comprises a shell, universal wheels are fixedly mounted at the four corners of the bottom of the shell correspondingly, supporting legs are arranged at the four corners of the bottom of the shell, a motor is fixedly mounted on one side of the inner wall of the bottom of the shell, an output shaft of the motor is fixedly sleeved with a first belt wheel, and a first shaft is rotatably mounted on one side of the shell. According to the marking device, fasteners can be effectively fixed through fixing pieces, the fasteners can be rapidly and efficiently marked through mutual cooperation of an incomplete gear, a chain, a hydraulic rod and other components, operation is easy, use is convenient, and the marking device is suitable for application and popularization.

Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20° C. and 100° C. inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures.

The invention relates to a cold water coral distribution prediction method and system based on sample selection expansion. According to the method, firstly, a training set is generated by adopting a random uniform sampling method, and the problem that ocean coral distribution prediction lacks negative samples is solved; secondly, a radial basis function neural network prediction model is trained through the training set, and unlabeled samples are marked by using the trained model; meanwhile, the steps are cycled for multiple times, and accidental factors of single prediction are eliminated. The concept of priori knowledge is introduced into the method, and the label-free prediction results of the previous cycle are grouped; and then, according to the principle that the higher the probability of the positive sample is, the higher the confidence is, different groups are put into the reconstructed virtual label-free sample set according to different proportions, and the next cycle is entered. Finally, unlabeled samples are classified according to the average positive sample probability to realize prediction of cold water coral distribution.

The invention provides a device for marking the axis center point of a pipeline bent section. The device comprises a machining piece A, a machining piece B, a machining piece C, a machining piece D and a pulling wire; the lengths of the machining piece A and the machining piece B are the same, and the lengths of the machining piece C and the machining piece D are the same; one end of the machining piece A is movably connected with one end of the machining piece B, and the other end of the machining piece A is movably connected with one end of the machining piece D; the other end of the machining piece D is movably connected with one end of the machining piece C, and the other end of the machining piece C is movably connected with the other end of the machining piece B; and one end of the pulling wire is fixed to the connecting position of the machining piece C and the machining piece D, and the other end of the pulling wire is the free end. According to the device, the characteristic that the straight line where the diagonal line of a pair of deltoid unequal angles is located serves as a symmetric axis is utilized, efficient and accurate marking of the axis center point of the pipeline bent section is achieved, and the problem that the mode that the center line of the pipeline bent section is marked by personnel is low in efficiency and poor in accuracy is solved; and particularly through the wire pulling mode, the axis center point of the pipeline bent section can be accurately marked on pipelines with different diameters.

The invention provides a defect detection wafer graph optimization method and an optimization system thereof. The defect detection wafer graph optimization method comprises the following steps of: performing defect detection on a wafer through a machine end to obtain a wafer graph; carrying out probe testing on each chip on the wafer, and importing probe testing data of the chips into the machine end; generating a wafer probing map according to the probing data of each chip; and making the proportion and coordinates of the wafer prober map and the wafer map consistent so as to compare the wafer prober map and the wafer map, and marking chips which are not in the wafer prober map in the wafer map. According to the defect detection wafer graph optimization method, the prober data is imported into the machine end, the wafer prober map is generated, the wafer prober map is compared with the wafer map of the machine end, invalid chips can be marked efficiently and accurately, optimization of the wafer map is achieved, the capacity of the machine is released, time is saved, and manpower consumption is reduced.

The invention discloses a multifunctional tumor injector. The multifunctional tumor injector comprises an injector body and a puncture needle body. An injector cylinder is arranged in the injector body. A puncture needle is arranged in the puncture needle body. A puncture needle sleeve is connected to the outer side of the puncture needle detachably. A first puncture needle barb is arranged on thelower portion of the puncture needle. A second puncture needle barb is arranged on the upper portion of the puncture needle. A puncture needle through hole is formed in the lower portion of the puncture needle. A puncture needle in-vitro marker is arranged at the top of the puncture needle. The multifunctional tumor injector has the advantages that the injector body and the puncture needle are arranged, and the puncture needle is connected with the injector body flexibly through a fastener, so that the position of a tumor of a patient can be marked and fastened by multiple angles; the markingbarb and the auxiliary fastening barb are arranged, so that the tumor can be marked and fastened efficiently; through the through hole and a flap capable of being opened in a unidirectional way, marking liquor can be supplemented at any time, so that the capability of efficient tumor marking is guaranteed.

The invention discloses an 89Zr drug labeling and purifying device. The device comprises a pressure device, a to-be-labeled drug solution raw material bottle, a chelating agent solution raw material bottle, an 89Zr solution raw material bottle, a product bottle, two reaction kettles, two purification columns, two buffer solution raw material bottles, at least five three-way valves and at least two one-way valves. The 89Zr drug labeling and purifying device provided by the invention can be automatically controlled, is simple to operate, is safe and convenient, and can effectively reduce the ionizing radiation exposure of operators; the labeling process and the purification process can be carried out in the device, isotope pollution is not prone to occurring, and 89Zr medicine labeling and purification can be efficiently, rapidly and accurately completed; the device provided by the invention is used for labeling and purifying the 89Zr medicine, the operation is easy, the marking can be efficiently and quickly completed, and the radiochemical purity of the marked medicine can reach 95% or above.

The invention discloses a 18F labelled precursor compound of which structural formula is shown as below, wherein R1 is hydrogen, methyl, ethyl, propyl, isopropyl and butyl, n is an integer ranging from 1 to 5; and F is 19F or 18F. The invention also discloses a preparation method of the compound. The method comprises the following steps in turn: 1) the synthesis of the labelled precursor compound: dissolving 3,4-dinitrobenzoic acid, amino alkyne, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 1-hydroxybenzotriazole in dimethylformamide, stirring to react; postprocessing the product of the reaction to obtain a precursor compound; and 2) the labelling of the precursor compound. The 18F labelled precursor compound can be used in the clickchemistry method to label amino acidsand polypeptide compounds and can also be used as molecular imaging probe in the positron emission tomography (PET) mapping.

The invention belongs to the technical field of isotopic tracing, and relates to the field of basic research of algae, in particular to a method for efficiently extracting an isotopic labeled chlorella soluble extracellular polymeric substance. Comprising the following steps: preparing a culture medium; culturing chlorella to obtain biomass; extracting an S-EPS concentrated solution and a B-EPS concentrated solution; enriching and concentrating the chlorella isotope labeled extracellular polymeric substance; performing dialysis; and cooling and drying to obtain chlorella isotope labeled extracellular polymeric powder. According to the method, efficient and rapid labeling of the chlorella extracellular polymeric substance is realized, the use of isotopes is saved, and the cost is saved. The rapid concentration of the efficiently labeled soluble extracellular polymeric substance is realized, the workload of subsequent dialysis is reduced, and the extraction efficiency is improved.

The invention discloses a marker acting on a human epidermis and used for carrying out marking before radiotherapy. The marker comprises a shell and a pen point. The marker comprises the handheld shell and the pen point, wherein a solution box is mounted in the shell, a gentian violet solution is contained in the solution box, a valve plate is slidably connected in the solution box, the valve plate is fixedly mounted at the axis of the shell through a vertical rod, and a pressing plate slides downwards to drive the solution box to slide downwards. According to the marker acting on the human epidermis and used for carrying out marking before the radiotherapy, a supply structure of a dyeing solution is redesigned, the pen point in the marker can stably discharge liquid to achieve a marking purpose in a one-time-pressing continuous liquid-supply mode, and the purpose of direct marking without contacting the skin of a patient can be achieved by taking the same pressing acting force as a driving force, so that suffering increase caused by direct contact between the skin and a marking structure after the skin is damaged by the radiotherapy is avoided, and the marking effect is clearer and more complete.

The invention discloses an alkylation derivatization reagent and an application of the alkylation derivatization reagent in peptide fragment marking and mass spectrometric detection. A molecular formula of the alkylation derivatization reagent is brominated 1-[3-[(2-iodine-1-oxo-ethyl) amino] propyl]-3-butyl imidazole (IPBI), and a structure thereof is as shown in Formula (I). The alkylation derivatization reagent takes an iodoacetamide base as an active functional group, and has high hydrophobicity, high gas phase alkalinity and a structural feature of containing permanent positive charges; selective derivatization and efficient mark of active sulfhydryl in polypeptide / protein can be achieved; the charge number of a peptide fragment, the ionization efficiency and the detection sensitivity in mass spectrometric analysis can be increased and improved remarkably, so that a detection rate of a cysteine peptide fragment is increased remarkably; and the alkylation derivatization reagent is simple in synthetic process, lower in cost, good in reaction reproducibility, and convenient to popularize and apply.

The invention discloses a binding protein for labeling beta-D glucan and a purifying and synthesizing method of the binding protein. The purifying and synthesizing method of the binding protein comprises the steps of transformation of a vector system and optimized induction to an expression strain, screening and culturing on the high-expression strain after a small amount of tests, and a large amount of protein purification, synthesis and elution. The constructed expression vector is transformed into the expression strain, bacterial colonies are screened and PCR sequencing verification is carried out to obtain a positive strain, after a small amount of preliminary experiments are carried out, SDS-PAGE is carried out meanwhile to confirm expression of a target protein and screen out the high-expression strain, then a large number of high-expression strains are cultured, and a large amount of protein purification is further carried out. The binding protein and beta-D glucan as a fungal cell wall can be subjected to immunoaffinity effectively, and fungal surface beta-D glucan antigen is labeled accurately.

The invention discloses a ferrimagnetic nanometer material, which comprises cubic iron ferric oxide nanometer particles and phospholipid polyethylene glycol coated on the outer layer of the cubic iron ferric oxide nanoparticle. The invention also discloses the application of ferrimagnetic nanometer material in magnetic particle imaging. The ferrimagnetic nanometer material provided by the invention can be stably dispersed in an aqueous phase system, has excellent magnetic particle imaging effect, and is a sensitive and efficient MPI contrast agent.

The invention discloses an identification method for genetic purity of a hybrid seedling of pear. The identification method comprises the following steps: (1) extracting a pear seedling genome DNA; (2) with nucleotide sequences shown in SEQ ID NO:1-2 as primers, carrying out PCR amplification by employing the pear seedling genome DNA obtained from the step (1) as a template to obtain an amplification product; (3) detecting the amplification product obtained from the step (2) by employing non-denaturing polyacrylamide gel electrophoresis; and (4) analyzing the gel electrophoresis result, and if strips of which the sizes are 60bp and 90bp simultaneously appear in gel electrophoresis, determining that the seedling is the hybrid seedling 'sucui No.1'. The method has the advantages of being stable to mark, high in accuracy, and low in cost, is not affected by the growth stage of a tested sample and the environment, and can be carried out in the overall growth season; and the genetic purity of the hybrid seedling 'sucui No.1' of the pear can be accurately identified within a short period of time.

The invention discloses a carbon-11 ( 11 C) The radiopharmaceutical and its preparation method and application relate to the field of medical technology. use 11 C-labeled positron radiopharmaceuticals, including 11 C‑FAPI‑01 and 11 C-FAPI-02, the chemical names are (S)-N-(2-(2-cyanopyrrolidin-1-yl)-2-oxoethyl)-6-(methoxy- 11 C) quinoline-4-carboxamide and (S)-N-(2-(2-cyano-4,4-difluoropyrrolidin-1-yl)-2-oxoethyl)-6-( Methoxy- 11 C) quinoline-4-carboxamide; its preparation includes synthesis and separation and purification methods; the radiopharmaceutical can be used as an imaging agent for positron emission tomography PET / CT inspection. The radiochemical synthesis reaction condition of the invention is mild, the synthesis time is fast, the radiochemical yield is high, and the fibroblast activation protein can be targeted.

The invention discloses a bottom surface appearance inspector. The bottom surface appearance inspector comprises an inspection frame, wherein a transmission mechanism is arranged on two sides of the inspection frame; a guide rail is arranged at one end of the inspection frame and is sleeved with an identification mechanism; a shooting device is arranged just under the inspection frame. The bottom surface appearance inspector disclosed by the invention has the characteristics of simple structure and convenience in maintenance, can be used for efficiently and quickly inspecting the bottom surface of a product, can quickly carry out marking, and is in accordance with an industrial assembly line production process.

The invention discloses an identification method for genetic purity of a hybrid seedling of pear. The identification method comprises the following steps: (1) extracting a pear seedling genome DNA; (2) with nucleotide sequences shown in SEQ ID NO:1-2 as primers, carrying out PCR amplification by employing the pear seedling genome DNA obtained from the step (1) as a template to obtain an amplification product; (3) detecting the amplification product obtained from the step (2) by employing non-denaturing polyacrylamide gel electrophoresis; and (4) analyzing the gel electrophoresis result, and if strips of which the sizes are 60bp and 90bp simultaneously appear in gel electrophoresis, determining that the seedling is the hybrid seedling 'sucui No.1'. The method has the advantages of being stable to mark, high in accuracy, and low in cost, is not affected by the growth stage of a tested sample and the environment, and can be carried out in the overall growth season; and the genetic purity of the hybrid seedling 'sucui No.1' of the pear can be accurately identified within a short period of time.

The invention discloses a mall data statistical analysis system for electronic commerce, which comprises a box body, a box door, a filter screen and a central processing unit, a ventilation net I is arranged on one side wall of the box body, and a ventilation net II is arranged on the side wall, corresponding to the ventilation net I, of the box body. The system has the beneficial effects that through the design of a data sales situation trend summarizing module, a product sales and life cycle module, a member level and national sales distribution multi-angle sales analysis module, a keyword operation analysis module and a mall page access click data analysis module, the data analysis accuracy is improved; the data statistical analysis system can have the functions of efficiently collecting relevant data and summarizing relevant important parameter indexes, the working efficiency of the system is improved, the data statistical analysis system can have the rapid heat dissipation function through the design of the first ventilation net and the second ventilation net, the temperature in the system can be kept stable during long-time work. Therefore, the working and connecting states of all parts are prevented from being influenced.

The present disclosure relates to a nucleic acid identification method. The identification method comprises the steps of: a. contacting the sample with a hybridization probe, b. optionally, eluting the hybridization probe not bound to the target nucleic acid, and c. identifying whether the hybridization probe is bound to the target nucleic acid binding; wherein the hybridization probe comprises a reporter group, and the state of the hybridization probe is different in the following two cases, so that a recognizable signal is generated by the reporter group: (i) the hybridization probe The needle is free and (ii) the hybridization probe is bound to the target nucleic acid. The present disclosure also relates to the use of hybridization probes for fluorescent microscopic imaging of target nucleic acids. The disclosed identification method can simplify the operation steps of nucleic acid identification, efficiently label target nucleic acid molecules, reduce the non-specific binding between probes and nucleic acids other than target nucleic acids, improve the efficiency and accuracy of identification, and is especially beneficial for short length and and / or identification of non-repetitive nucleic acids, suitable for obtaining super-resolution images of target nucleic acids.

The invention provides new application of near-infrared II area quantum dots containing selenium elements. According to the new application, the near-infrared II area quantum dots are used for T cellmarking and / or T cell in-vivo tracking and contain the selenium elements. According to the new application of the near-infrared II area quantum dots containing the selenium elements, by utilizing natural selenium element transfer systems of T cells, the T cells take in the near-infrared II area quantum dots containing the selenium elements, natural, nondestructive and efficient marking of the T cells is achieved, and the marked quantum dots are evenly distributed in T cell cytoplasm; by utilizing a laser confocal microscope or a small animal in-vivo imaging system, in-vivo tracking of the T cells is achieved through near-infrared II area fluorescence signals; and efficient and nondestructive marking of the T cells can be achieved without using expensive bioactive molecules to modify the quantum dots.