New application of near-infrared II area quantum dots containing selenium elements

Abstract

The invention provides new application of near-infrared II area quantum dots containing selenium elements. According to the new application, the near-infrared II area quantum dots are used for T cellmarking and / or T cell in-vivo tracking and contain the selenium elements. According to the new application of the near-infrared II area quantum dots containing the selenium elements, by utilizing natural selenium element transfer systems of T cells, the T cells take in the near-infrared II area quantum dots containing the selenium elements, natural, nondestructive and efficient marking of the T cells is achieved, and the marked quantum dots are evenly distributed in T cell cytoplasm; by utilizing a laser confocal microscope or a small animal in-vivo imaging system, in-vivo tracking of the T cells is achieved through near-infrared II area fluorescence signals; and efficient and nondestructive marking of the T cells can be achieved without using expensive bioactive molecules to modify the quantum dots.

Description

technical field [0001] The invention belongs to the technical field of biological imaging and relates to a new application of quantum dots in the near-infrared II region containing selenium element. Background technique [0002] T cells are a type of lymphocytes and are an important component of the adaptive immune system. With the assistance of antigen-presenting cells, T cells can recognize and kill virus-infected cells or cells with abnormal gene expression (such as cancer cells), and play an important role in fighting against external pathogen invasion and tumor immunotherapy. [0003] Tumor immunotherapy is an emerging precise and effective tumor treatment method, mainly including immune node check inhibitor therapy and adoptive T cell therapy. Among them, adoptive T cell therapy is to modify T cells from the patient's own or allogeneic body in vitro to make them have the ability to kill tumors, and then infuse them back into the patient's body to achieve the killing e...

AI Technical Summary

Problems solved by technology

For example, CD3 antibody can be modified on the surface of quantum dots, and T cell labeling can be completed by co-culturing with T cells for 30 minutes to obtain a better labeling effect. The disadvantage is that the antibody is expensive, the material needs to be modified, and the operation is cumbersome. The reaction with the ligand occurs only on the cell surface and the process is reversible, resulting in unstable labeling and possible shedding during tracking

Quantum dots modified by membrane-penetrating peptides can efficiently enter the interior of cells, and the labeling effect is stable. However, membrane-penetrating peptides are expensive, which greatly increases the cost of probe preparation, and the mechanism of action of membrane-penetrating peptides is to forcibly penetrate the membrane. Has a damaging effect, which is not conducive to long-term tracking of cell migration

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