Identification method for genetic purity of hybrid seedling of pear

Abstract

The invention discloses an identification method for genetic purity of a hybrid seedling of pear. The identification method comprises the following steps: (1) extracting a pear seedling genome DNA; (2) with nucleotide sequences shown in SEQ ID NO:1-2 as primers, carrying out PCR amplification by employing the pear seedling genome DNA obtained from the step (1) as a template to obtain an amplification product; (3) detecting the amplification product obtained from the step (2) by employing non-denaturing polyacrylamide gel electrophoresis; and (4) analyzing the gel electrophoresis result, and if strips of which the sizes are 60bp and 90bp simultaneously appear in gel electrophoresis, determining that the seedling is the hybrid seedling 'sucui No.1'. The method has the advantages of being stable to mark, high in accuracy, and low in cost, is not affected by the growth stage of a tested sample and the environment, and can be carried out in the overall growth season; and the genetic purity of the hybrid seedling 'sucui No.1' of the pear can be accurately identified within a short period of time.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying the genetic purity of pear hybrid seedlings, which uses SRAP molecular marker technology to detect the genetic purity of pear variety 'Sucui No. 1' seedlings. Background technique [0002] Hybrid breeding occupies an important position in crop breeding. In recent years, many excellent varieties have been cultivated in agriculture by using hybrid breeding, and fruitful results have been achieved in woody plants. The position in production is becoming more and more important, and the role they play is also increasing. At the same time, the seedling market is mixed with good and evil people, and the inferior ones fill up the superior ones, and the false ones happen from time to time. False advertisements are widely spread in various media, which not only damages the interests of farmers, but also poses a great threat to fruit tree production and ecol...

AI Technical Summary

Problems solved by technology

[0003] In the early days, people used morphological methods to identify varieties, mainly including field planting identification and isoenzyme identification. However, field identification requires sowing and raising seedlings, transplanting them into the field, and then observing the morphological characteristics, which is time-consuming, heavy workload, and easy. Affected by seasons and environments, and with the centralized use of breeding parents, the new varieties bred are more similar in more traits and difficult to distinguish, sometimes due to the morphological differences between true and false pear seedlings The performance is not very obvious, and it is difficult to identify at the seedling stage, especially when the genetic basis between the parents is very close, it is even more difficult to identify

The isoenzyme analysis is easily affected by the environment and the part of the material taken, and for some hybrid varieties with small genetic differences between the parents, it is sometimes difficult to detect and identify the isozyme for purity, and the accuracy is not high

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