The present invention discloses a Stauntonia obovatifoliola propagation method, which comprises: S1, making sterile explants of Stauntonia obovatifoliola; S2, culturing the sterile explants with an induced culture medium to obtain axillary buds; S3, culturing the axillary buds with a proliferation culture medium to obtain clustered shoots; S4, culturing the clustered shoots with a strong seedling culture medium to obtain Stauntonia obovatifoliola seedlings; S5, culturing the Stauntonia obovatifoliola seedlings with a rooting culture medium to obtain the rooted Stauntonia obovatifoliola seedlings, wherein the rooting culture medium contains MS, 0.3-1.3 mg / L of indole butyric acid and 0.1-0.5 g / L of active carbon; and S6, transplanting the rooted Stauntonia obovatifoliola seedlings into a matrix to culture. According to the present invention, with the tissue culture and the matrix cultivation, the wild plant Stauntonia obovatifoliola is successfully cultivated; and the reaction conditions such as the temperature, the time and the light intensity can be regulated, the obtained Stauntonia obovatifoliola has the high survival rate, the scale production of the Stauntonia obovatifoliola is solved, and the cultivation time of the Stauntonia obovatifoliola is shortened.