Method for reducing content of phenylalanine in product
A low phenylalanine and phenylalanine technology is applied in the field of food processing to achieve the effects of improving adsorption removal rate, good mechanical strength, economical and environmental protection resources
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Embodiment 1
[0029] S1 extracts peanut protein: Weigh a certain amount of 121.4 g / mol Tris to dissolve, tune with concentrated hydrochloric acid, diluted to 20 mm, and quantify 25 ml of spare. 5 g of peanut protein powder was weighed, dissolved in Tris buffer, and the magneton was added, and the magnetic stirrer was oscillated with a rotational speed of 500 rpm for 2 h. Then, in 50 mL of the centrifuge tube, centrifuge at 3000 × g, 4 ° C, was centrifuged, and the supernatant was placed in a high-speed centrifuge tube, centrifuged at 10,000 × g, 4 ° C for 30 min, and the supernatant was made into a petroleum solution. .
[0030] (2) Enzymatic peanut protein: Take 10 ml of peanoglycin solution, add 90 ml of distilled water to 10 times, at 90 ° C heat treatment for 20 min, heat treatment, cool to 60 ° C, adjust the pH to 8.0, add 3500 u / G alkaline protease hydrolysis 110 min, hydrolysis After completion, the boiling water bath is escaping 10 min; cooling to 50 ° C, adjusting pH to 7.0, adding...
Embodiment 2
[0035] (1) Extraction of peanut protein: Weigh a certain amount of 121.4 g / mol Tris to dissolve, tonifying the pH of 7.2 with concentrated hydrochloride, diluted to 20 mm, and quantified 25 ml of spare. 5 g of peanut protein powder was weighed, dissolved in Tris buffer, and the magneton was added, and the magnetic stirrer was oscillated with a rotational speed of 500 rpm for 2 h. Then, in 50 mL of the centrifuge tube, centrifuge at 3000 × g, 4 ° C, was centrifuged, and the supernatant was placed in a high-speed centrifuge tube, centrifuged at 10,000 × g, 4 ° C for 30 min, and the supernatant was made into a petroleum solution. .
[0036] (2) Enzymatic peanut protein: Take 10 ml of peanoglycin solution, add 90 ml of distilled water to 10 times, at 90 ° C heat treatment for 20 min, heat treatment, cool to 60 ° C, adjust the pH to 8.0, add 3500 u / G alkaline protease hydrolysis 110 min, hydrolysis After completion, the boiling water bath is escaping 10 min; cooling to 50 ° C, adju...
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