30 results about "Tuberculosis bacillus" patented technology

The invention provides a tubercle bacillus drug tolerance detection reagent kit and method. The tubercle bacillus drug tolerance detection reagent kit comprises a tubercle bacillus drug tolerance detection reagent, wherein the tubercle bacillus drug tolerance detection reagent comprises a sequencing primer in accordance with a tubercle bacillus drug tolerance gene; the tubercle bacillus drug tolerance gene comprises one or more genes of rpoB, katG, inhA-promoter, inhA-structural, furA, embB, ubiA, pncA, rpsA, gyrA, gyrB, eis, rpsL, rrs, tlyA, rplC and rrl; and further, the reagent kit also contains a tubercle bacillus nucleic acid detection reagent, and the tubercle bacillus nucleic acid detection reagent comprises a primer pair 1 in accordance with IS6110, a primer pair 2 in accordance with the IS6110 and a probe primer in accordance with the IS6110. Through the adoption of the tubercle bacillus drug tolerance detection reagent kit disclosed by the invention, tubercle bacillus nucleicacid in samples can be quickly detected, and positive samples can be further subjected to drug tolerance detection; and the tubercle bacillus drug tolerance detection reagent kit has good sensitivity, good specificity and good accuracy, and can perform mutation detection on 48 sites of 17 drug tolerance genes of common antituberculosis drugs and fragment deficiency detection of an intergenic region, so that the tuberculosis medication can be more accurately and comprehensively guided.

The invention discloses a catechu extract composition for resisting tubercle bacillus, a preparation method of the catechu extract composition, a pharmaceutical preparation containing the catechu extract composition, and application of the catechu extract composition. The catechu extract composition contains a flavanoid compound of which the purity is more than 50%, and the flavanoid compound takes catechin and / or epicatechin as main components. The main structural formulas (I) and (II) of the flavanoid compound are shown in the specifications, wherein R1, R2, R3, R4 and R5 are respectively and independently selected from hydrogen, C1-C6 alkyl, beta-D-glucopyranesyl, SO3 or PO3. By adopting a chromatographic process for refining and purifying the catechu extract composition disclosed by the invention, the effective content is as high as 99.5%. The catechu extract composition and the pharmaceutical composition thereof have excellent effects on improving or treating pulmonary tuberculosis, treating ulcerative bone tuberculosis and AIDS (Acquired Immune Deficiency Syndrome) complicated by tuberculosis, treating drug resistant tuberculosis, and especially treating drug resistant tuberculosis and AIDS complicated by tuberculosis.

The invention relates to an isoniazid dry powder inhalant for treating pulmonary tuberculosis. The isoniazid dry powder inhalant comprises isoniazid micro-powder and comprises or does not comprise carrier micro-powder; the particle size of the isoniazid micro-powder is treated to 0.1-10 mu m by a fluidized bed supersonic jet milling method or a spray drying method; preferably, auxiliary materialsincluding leucine, mannitol or phospholipid are added into the dry powder inhalant so as to enhance the pelletizing rate of particles; and furthermore, auxiliary materials including magnesium stearate, mannitol, leucine and / or lactose are added so as to improve the flowability of the medicine and reduce the particle agglomeration phenomenon. The preparation process of the isoniazid dry powder inhalant is simple; and the isoniazid dry powder inhalant with relatively high stability can be prepared. The dry powder inhalant can effectively deliver drugs to pulmonary alveoli so as to effectively kill and inhibit tubercle bacillus in respiratory tracts, and is particularly suitable for treating pulmonary tuberculosis patients with impaired liver functions.

The invention belongs to the field of biological gene engineering, and aims to solve the technical problems that in the prior art, a vaccine for treating pig encephalitis B is low in virus titer and poor in holding capacity. The invention provides a fusion protein which consists of a neutralizing epitope gene of a C-terminal of an encephalitis-B E protein and an M.Thsp70 gene. The invention also provides a vector containing the fusion protein gene, a vaccine containing the fusion protein and the preparation method of the vector. The preparation method of the vector comprises the following steps of: firstly, synthesizing the neutralizing epitope gene of the C-terminal of the encephalitis-B E protein, and cloning the neutralizing epitope gene into a prokaryotic expression vector; secondly, amplifying by taking a genome of a bacillus tuberculosis H37Rv standard strain as a template to obtain the M.Thsp70 gene, and cloning the M.Thsp70 gene into the prokaryotic expression vector to obtain a fusion protein expression vector; and finally, purifying to obtain an expression protein for preparing the vaccine which is used for preventing the encephalitis B. The titer of the neutralizing antibody of the vaccine provided by the invention is higher than that of a lentogenic vaccine, and is easy to produce efficiently and quickly on a large scale.

A combined drug for inhibiting multidrug-resistant tuberculosis bacilli comprises ethyl acetate fraction of forsythiae, isoniazid and rifampicin for simultaneous or separate administration, and pharmaceutically acceptable carriers. The combined medicine of the invention can obviously reduce the dosage of isoniazid and rifampicin, remarkably increase the synergistic bacteriostatic effect, thereby reducing the toxic and side effects of isoniazid and rifampicin, increasing the medication compliance of patients, and providing a new choice for clinic.

The invention discloses a compound paclitaxel anti-tuberculosis capsule tablet preparation method and process, and the process comprises the following steps: step 1, taking a certain amount of paclitaxel extract for dilution, and carrying out separation and passivation treatment on the diluted paclitaxel extract; step 2, carrying out elution treatment on the separated and passivated paclitaxel extract, and carrying out recovery and concentration on the eluted paclitaxel. The paclitaxel anti-tuberculosis capsule tablet has the beneficial effects that the prepared paclitaxel anti-tuberculosis capsule tablet directly kills tubercle bacillus and cancer cell tissues by adopting toxic attack, protects normal cells, is free of toxic reaction, high in curative effect, short in medication time, free of damage to the liver. Meanwhile, the paclitaxel anti-tuberculosis capsule tablet can stimulate and adjust the immune defense function in vivo, enhance the digestion and absorption functions and improve the overall defense function while treating, so that the treatment of tuberculosis is more efficient and safer.

A Chinese medicine in the form of particle or others for treating pulmonary tuberculosis is prepared from 6 Chinese-medicinal materials including dandelion herb, fleece flower root, stemona root, bletilla tuber, etc. Its preparing process is also disclosed.

The invention belongs to the technical field of pharmaceutical chemistry, and especially relates to use of compound AG120 or pharmaceutically acceptable salt thereof in preparation of drug for prevention or treatment of tuberculosis, and a pharmaceutical composition for preventing or treating tuberculosis. The compound AG120 can effectively inhibit the infection of tuberculosis bacillus, thereby providing effective drug support for the prevention or treatment of tuberculosis. Therefore, the present invention discloses compound AG120 which can be used as a lead compound for preventing or treating tuberculosis, and has the activity of anti-tuberculosis bacteria and drug-resistant tuberculosis bacteria, and is particularly suitable for preventing or treating tuberculosis, thus having wide clinical application prospect and great market potential.

The invention belongs to the biomedical field, in particular to the use of Bacteroides fragilis in the preparation of drugs for the treatment and prevention of tuberculosis. The invention also provides the application of Bacteroides fragilis in the preparation of pharmaceutical compositions, foods, health products and food additives for treating and / or preventing tuberculosis. The Bacteroides fragilis provided by the invention can effectively treat and / or prevent tuberculosis and enhance the immune protection effect of tuberculosis bacillus Calmette-Guerin vaccine (BCG), and has wide application prospect.

The present invention relates to the technical field of bio-pharmaceuticals, and particularly relates to an application of TREM-2+T cells in preparation of drugs for treating tuberculosis. The application of TREM-2+T cells has the following beneficial effects: the TREM-2+T cells are applied to prepare drugs treating tuberculosis for the first time, and the drugs can achieve immunotherapy on the tuberculosis, relieve pulmonary tuberculosis bacteria and reduce inflammation. In addition, since the TREM-2+T cells are derived from the autologous peripheral blood of a patient without rejection, thereaction is suitable for comprehensive treatment of tuberculosis. According to the invention, the TREM-2+T cells for treating the tuberculosis are prepared by processes such as flow cytometric sorting, in-vitro culture amplification stimulated by interleukin 2 and washing, and the preparation method is more scientific and efficient.

The invention relates to quinoline compounds, a preparation method and application thereof, and specifically provides a class of novel quinoline compounds, a pharmaceutically acceptable salt and a preparation method thereof, and application in preparation of medicines for treating tubercle bacillus infectious diseases, particularly infectious diseases caused by drug-resistant tubercle bacillus. The quinoline compound or the pharmaceutically acceptable salt thereof provided by the invention has good anti-tubercle bacillus activity, and especially has strong activity on drug-resistant tubercle bacillus.

The invention belongs to a medicament for treating bone tuberculosis, and discloses a medicament which takes Chinese medicinal herbs as raw materials and is used for treating bone tuberculosis and a preparation method thereof. The medicament comprises the following components in certain proportion: creeping woodsorrel herb, Chinese brake herb, eucommia bark, european verbena, frost phlorone, fortune's drynaria rhizome, dock root, herb of cavalerie mosla and typhonium giganteum engler. The components are dried in the sun and then are ground into medicinal powder to prepare pharmaceutically acceptable formulations. The medicament for treating the bone tuberculosis can inhibit the growth of tubercle bacillus, has the effects of tonifying the liver and kidney, subdhing swelling and detoxicating and strengthening the muscles and bones, and has the obvious curative effect on the bone tuberculosis; and simultaneously, the medicament has no side effect, does not need to be used together with other medicaments, and does not generate medicament tolerance. Compared with compatible medicaments, the medicament has a low cost and can cure the bone tuberculosis in a short time about 50 to 80 days.

A Chinese medicine in the form of pill for treating pulmonary tuberculosis is prepared from verdigris, alum, gypsum, zedoary, wheat bran and / or flour, and edible vinegar through grinding the first four, mixing, adding heated vinegar, adding wheat bran and / or flour, stirring , pilling and baking.

The embodiment of the invention provides a method and a device for identifying the tuberculosis bacillus, which relate to the field of microorganism identification. Digital images are obtained by scanning sputum smears of Mycobacterium tuberculosis with a Mycobacterium tuberculosis scanner. A magnify digital picture obtained by enlarge that digital picture is compared with a sample picture of conjugated bacilli in a preset tuberculosis bacillus database. When the scanner scans the sputum smear of Mycobacterium tuberculosis to obtain a digital picture, only one objective lens is used to scan the digital picture, and the digital picture is enlarged to obtain an enlarged digital picture. The whole process does not require the use of a higher objective lens, and there is no switching back andforth between high and low objective lenses, and the process is relatively simple, and the whole scanning process uses only one objective lens to scan, saving time.

The invention discloses a preparation method and application of a MC-ARMS-mMB triplet technology in a drug-resistant tuberculosis diagnosis kit, and describes the MC-ARMS-mMB (microcolony-amplification-impeding mutation system-multiplex molecular beacon) triplet technology , a diagnostic kit for rapid culture of Mycobacterium tuberculosis and its drug-resistant bacteria. At the same time, the preparation method of the diagnostic kit was clarified; and a scientific presentation was made on its clinical application. It is particularly important to point out the clinical application value and better development prospect of the diagnostic kit.

The invention relates to a processing method of croton for treating tuberculosis, in particular to a high-temperature processing and attenuation method for traditional Chinese medicine croton and action thereof on treatment of the tuberculosis. The croton is processed through three methods of high-temperature frying, high-temperature roasting and microwave heating to obtain a croton processed product. In the processed croton, toxic protein is not detected, LD50 is obviously increased, the relative content of each component in croton oil is basically kept unchanged, and the croton oil content is relatively increased. After being combined with other traditional Chinese medicines to prepare pills, the processed croton has an obvious anti-tuberculosis bacillus effect in vivo and in vitro. Theprocessing method of the croton for treating the tuberculosis disclosed by the invention has the effects of reducing toxicity and enhancing therapeutic effect; and compared with the conventional croton processing methods of roasting, boiling, steaming and the like, the processing method has the advantages of prominent advantages.

The invention relates to a bone rehabilitating material and a preparation method of the material. The invention provides a bone rehabilitating material, the effective components of which are prepared by the following weights and portions of raw materials: 40 of a PLGA; 20 to 80 of a semi-hydrated calcium sulphate; 2 to 8 of a rifampicin. The invention also provides the preparation method of the bone rehabilitating material. The invention mixes and solidifies the semi-hydrated calcium sulphate, the PLGA, the rifampicin and a sodium chloride granule and digests the sodium chloride granule to make a novel multi-hole bone rehabilitating material with a function of anti-bacillus tuberculosis.

The invention relates to the technical field of traditional Chinese medicines, in particular to a medicine for treating pulmonary tuberculosis, which is prepared from Japanese raspberry root, radices stellariae dichotomae, sweet wormwood herb, bletilla striata and large-leaved gentian. The preparation method comprises three steps of decoction preparation, medicine powder preparation and compounding. Compared with the prior art, the medicine for treating pulmonary tuberculosis has the following beneficial effects that the medicine for treating pulmonary tuberculosis takes pure natural traditional Chinese medicines as raw materials, all the raw materials are in mutual compatibility in a monarch, minister, assistant and guide manner, and decoction and medicine powder are mutually matched, so that the effect of treating pulmonary tuberculosis can be achieved, and meanwhile, the medicine is rapid in effect, exact in curative effect and free of obvious side effects; liver and kidney function damage and drug resistance caused by western medicines are also avoided; meanwhile, the Japanese raspberry root is taken as a main component, medicine properties are extracted from the bottom of the Japanese raspberry root, and after the medicine is taken for one treatment course, the tuberculosis bacillus can be obviously cured, and the symptoms such as low fever, cough, hematemesis and abnormal sweating caused by existing symptoms are changed.

A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.

An air refreshing agent for killing the viruses and bacteria in air is prepared from "Yujiexin", alcohol, propanediol, essence, and distilled water through dissolving "Yujiexin" in alcohol, adding the solution and propanediol to distilled water, stilling, adding essence and stirring. Its advantages are high effect to kill grippe virus, tuberculosis bacillus, Bacterium diphtheriae, etc. and no by-effect to human body.

The present invention relates to a series of diphenyl oxide derivatives and a use of the diphenyl oxide derivatives in preparation of anti-tuberculosis drugs, and belongs to the field of chemical medicine. Specifically the present invention relates to a use of a compound represented by a formula (I), wherein Ar1 is a substituted benzene ring, n is an integer of 0-3, R1 is a substituted benzene ring Ar2 or a substituted heterocyclic Het. The test results show that the compound represented by the formula (I) provides good in vitro inhibition activity for tubercle bacillus H37Rv, provides a good treatment effect for in vivo BCG infection mice model, and provides a new selection for preparation of the anti-tuberculosis drugs.

The invention relates to a processing method of croton for treating tuberculosis, in particular to a high-temperature processing and attenuation method for traditional Chinese medicine croton and action thereof on treatment of the tuberculosis. The croton is processed through three methods of high-temperature frying, high-temperature roasting and microwave heating to obtain a croton processed product. In the processed croton, toxic protein is not detected, LD50 is obviously increased, the relative content of each component in croton oil is basically kept unchanged, and the croton oil content is relatively increased. After being combined with other traditional Chinese medicines to prepare pills, the processed croton has an obvious anti-tuberculosis bacillus effect in vivo and in vitro. Theprocessing method of the croton for treating the tuberculosis disclosed by the invention has the effects of reducing toxicity and enhancing therapeutic effect; and compared with the conventional croton processing methods of roasting, boiling, steaming and the like, the processing method has the advantages of prominent advantages.

The invention discloses avain tuberculosis hyper-immune serum and a preparation method thereof. The preparation method includes the steps that inactivated avain tuberculosis bacilli are directly adopted as immunizing antigens, immunizing is conducted with an ear edge intravenous injection method, immunizing is conducted once every three days, immunizing is conducted seven times in total, blood sampling is conducted seven days after the last time of immunizing, and serum is collected. The high-potency serum can be prepared only through the inactivated bacilli via intravenous injection on immune animals, emulsifying operation is not needed, the whole hyper-immune serum preparation process only lasts for 26 days, the antibody valence measured by a tube agglutination test reaches 1 / 128, and compared with a conventional hyper-immune serum preparation method, the requirements for preparation conditions for the immunizing antigens involved in the method are low, immunizing procedure operation is simpler, needed time is shorter, and the high-potency antibody level can be acquired.

The invention discloses a use of chimonanthus plant anti-mycobacterium tuberculosis, and particularly relates to application of a chimonanthus plant and an extractive thereof for preparing pharmaceutical for anti-mycobacterium tuberculosis and diseases caused by the anti-mycobacterium tuberculosis. The chimonanthus plant comprises willow-pattern calyx canthus, Zhejiang calyx canthus and mountain calyx canthus. The extractive comprises a water extractive, an alcohol extractive, a steam distillation extractive and a supercritical extractive.

The present invention relates to the technical field of biopharmaceuticals, in particular to the application of TREM-2+T cells in the preparation of drugs for treating tuberculosis. The drug can realize immunotherapy for tuberculosis, reduce pulmonary tuberculosis, and reduce inflammation; in addition, because TREM-2+ T cells are derived from the patient's autologous peripheral blood, there is no rejection, and the reaction is suitable for comprehensive treatment of tuberculosis; TREM-2+ T cells for the treatment of tuberculosis are prepared through the process of cell sorting, interleukin-2 stimulation in vitro culture expansion and washing, and the preparation process is more scientific and efficient.

The invention discloses a tubercle bacillus fusion protein Mtb8.4-HspX, which is a protein shown as sequence 2 in a sequence table. The invention has the advantages that: the selected tubercle bacillus Mtb8.4 is a low molecular therapeutic protein antigen purified and separated from tubercle bacillus culture filtrate, has higher cell immunocompetence, and can induce immunoreactions of CD4+Th1 cells and generation of specific cytotoxic T lymphocyte (CTL) and secrete high-level interferon(IFN)-gamma; and HspX is a heat shock protein with the relative molecular mass of 16,000 and is a main protein generated by the tubercle bacillus in a stationary growth period or a low oxygen state. Specific humoral immune and cellular immune response to the HspX in bodies of tuberculosis patients and latent infected ones prove that the HspX is an important target antigen for organism immunoreaction in a latent tubercle bacillus infection period.