A provided quantitative determination method for fiber content of viloft fiber and acrylic fiber or spandex blended textiles comprises the following steps: S1, putting viloft fiber and acrylic fiber or spandex blended textiles in a baking box with a constant temperature of 102-108 DEG C to bake to dry, then rapidly taking out and putting in a dryer to cool to room temperature, and weighing the mass of the blended textiles and recording as m0; S2, putting the blended textiles in N,N-dimethylformamide or N,N-dimethylacetamide according to the sample (g) to reagent (mL) bath ratio of 1:100-200, continuously shaking at 90-100 DEG C for 5-30 min; and S3, washing fiber indissolvable in N,N-dimethylformamide or N,N-dimethylacetamide reagent in the step S2 with water to neutral, putting in a baking box with a constant temperature of 102-108 DEG C to bake to dry, rapidly taking out and putting in a dryer to cool to room temperature, and weighing the residual mass of the blended textiles and recording as m1. The dissolving temperature is controlled at 90-100 DEG C, and the method has extremely high accuracy and helps to substantially shorten the detection time.