73 results about "Nonhuman primate" patented technology

The invention discloses a multi-target small-interfering RNA cocktail preparation for treating ophthalmic diseases and the preparation method. The multi-target small-interfering-RNA cocktail preparation is composed of three or more than three small-interfering RNA aiming to three or more than three different genes and knocking down simultaneously a plurality of pathogenic genes; the multi-target small-interfering-RNA cocktail preparation is prepared by a plurality of small-interfering RNA in a certain proportion according to different diseases. The multi-target small-interfering-RNA cocktail preparation is a plurality of double-bond RNA molecules of different lengths from 19to 27nt, with blunt ends or overhanging ends; the RNA sequence in the multi-target small-interfering-RNA cocktail preparation has the homology to the gene targets of human, rat and other nonhuman primate; . The multi-target small-interfering-RNA cocktail preparation aiming to the following gene sequences: (1) virus-affection-related gene; (2) inflammation-arosing gene; (3) neovescular-related gene. The invention provides a novel treatment for a plurality of ophthalmic diseases, including retinopathy of prematurity, senile fundus macula lutea, retinopathy caused by senile diabetes, herpes simplex corneal stromal opacification and uveitis.

Systems, methods, and compositions for a diagnostic method for detecting TB in non-human primates that is easy to use, sensitive, and specific. The method utilizes recombinant mycobacterial antigens, such as polyfusion proteins. The method utilizes an antigen-antibody-antigen arrangement to detect TB infection in nonhuman primates. The method can detect IgM antibodies to TB, in addition to IgG antibodies, providing ability to detect TB earlier in nonhuman primate TB infection as compared to conventional TB tests.

The invention relates to a fixing chair for a nonhuman primate. The fixing chair comprises a chair seat (1) and a chair back (2). A pore structure is arranged on the chair back (2) and disposed above the chair seat (1). The pore structure is in fit with a box structure which is disposed at the back of a lab coat and used for accommodating a retaining tube. The pore structure is used for fixing the lab coat through the box structure to further fix the primate. According to the fixing chair, the body of the primate can be fixed conveniently, the retaining tube on the body surface can be protected against damage, but free motions of the neck and the limbs are not limited; besides, the fixing chair is of an open space structure, so that anxiety or hampering of observation towards the surrounding due to long-time binding of the primate is prevented, and accordingly the accuracy of monitoring of primate response behaviors in experiments is improved, and more real experiment results are obtained.

The invention discloses a feed for constructing a nonalcoholic steatohepatitis model of nonhuman primates and an application method of the feed. The feed comprises the following raw materials in partsby weight of 22-28% of soybean meal, 2.5-3.5% of Peru fish meal, 2-3% of feed-grade calcium hydrogen phosphate, 0.2-0.4% of salt, 0.1-0.3% of composite multiplex vitamins, 0.1-0.3% of high-stabilityVC, 0.4-0.6% of cholesterol, 20-25% of monocrystal levulose, 15-20% of animal oil and fat, 0.2-0.4% of methionine, 1-2% of premix minerals, and 30-35% of first-grade flour. A nonalcoholic steatohepatitis disease scoring system standard is used, and various individual index scores in a fibrosis scoring standard are obviously increased. After the feed is used for feeding the nonhuman primates for 3months, nonalcoholic steatohepatitis liver injury can be established, so that the nonalcoholic steatohepatitis animal model is formed.

The invention provides a method for establishment of a Mycobacterium tuberculosis naturally infected non-human primate model. According to the invention, a healthy non-human primate and a non-human primate artificially infected with tuberculosis and suffering cough are raised in an air flow self-circulation combined type negative pressure isolator so as to infect the healthy non-human primate with tuberculosis. Establishment of the natural infection model helps to reveal conditional factors (like HIV) activating latent tuberculosis infection and related mechanisms. The non-human primate model with more clinical significance is provided for research, development and evaluation of anti-tuberculosis vaccines and drugs.

The invention provides isolated non-human primate Fc receptor polypeptides, the nucleic acid molecules encoding the Fc receptor polypeptides, and the processes for production of recombinant forms of the Fc receptor polypeptides, including fusions, variants, and derivatives thereof. The invention also provides methods for evaluating the safety, efficacy and biological properties of Fc region containing molecules using the non-human primate Fc receptor polypeptides.

Compositions and methods for determining endometrial receptivity to embryo implantation and for detecting and treating castration-recurrent prostate cancer are provided. The methods comprise measuring the level of melanoma antigen gene protein-11 (MAGE-11, also referred to as MAGE-Al 1) in an endometrial or prostate tissue sample. The level of MAGE-11 protein or mRNA can be correlated to endometrial receptivity to embryo implantation in a female human or nonhuman primate, or to the presence of castration-recurrent prostate cancer in a male patient in need thereof. Methods are described whereby MAGE-11 may serve as a target for vaccine development in the treatment of castration-recurrent prostate cancer. Methods for monitoring endometrial maturation, for diagnosing infertility, and for in vitro fertilization in a female human or nonhuman primate are also provided. Compositions of the invention include antibodies that specifically bind MAGE-11 and oligonucleotide primers useful for detecting MAGE-11 mRNA, as well as kits containing such antibodies or primers.

Compositions and methods are provided for treating ocular disorders in a subject are provided. In one aspect, an adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding CNGA3. In another aspect, an adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding CNGB3. In another aspect, an adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding REP-1. In desired embodiments, the subject is human, cat, dog, sheep, or non-human primate.

The invention discloses a refrigeration preservation and resuscitation method of sperm of non-human primates. According to the refrigeration preservation and resuscitation method of sperm of non-human primates, in refrigeration preservation, sperm is subjected to liquefaction at normal temperature for 30min, and monkey sperm is diluted with HTF culture solution, wherein the volume ratio of the monkey sperm to the HTF culture solution is controlled to be 1:9; glycerin is added based on the volume of the monkey sperm until the volume final concentration of glycerin is 10%; an obtained mixture is subjected to subpackage in 1.5ml cryopreservation tubes, is stored at 4 DEG C for 30min, is stored for 30min in suspension above liquid nitrogen, and then is stored in liquid nitrogen; in resuscitation, the cryopreservation tubes stored in liquid nitrogen are shaken slightly in water bath at 37 DEG C for 1 to 3min until complete thawing is realized. Compared with the prior art, the survival rate and the fertility rate of the refrigeration preservation and resuscitation method are both increased; the refrigeration preservation and resuscitation method is effective, is rapid, and is convenient, and can be used for sperm refrigeration preservation and genetic resource conservation of other non-human primates.

The invention discloses a non-human primate behavioral data acquisition device and a data acquisition method.The non-human primate behavioral data acquisition device comprises an animal movement device, a plurality of stereo cameras and an analysis terminal, wherein the animal movement device comprises a running machine, a hanging bracket, an animal strait coat and a plurality of fluorescent paints; the hanging bracket is erected above a running belt of the running machine, a hanging rod extending towards the running belt is arranged on the hanging bracket, a first ring buckle is arranged at the tail end of the hanging rod, a second ring buckle is arranged on the animal strait coat, and the first ring buckle and the second ring buckle are correspondingly buckled. The plurality of fluorescent paints are used for marking the joints; the number of the stereo cameras is not less than four, and the stereo cameras are arranged on the peripheral area of the running machine at intervals and used for collecting animal gait data and transmitting the data to the analysis terminal. The non-human primate behavioral gait data can be effectively collected, and the non-human primate behavioral gait data can be analyzed.

The invention discloses a method for establishing a non-human primate autoimmune cerebrospinal meningitis model and the application of the model. The specific technical scheme comprises the following steps; a machin is selected as the non-human primate experimental animals; MOG34-56(100Mug / ml) is prepared into an emulsion (MOG CFA=1; 1); after the experimental machin is anesthetized, the machin is intradermally injected with 1ml of prepared emulsion at 10 injection points; on the seventh day after the first injection, an MOG-CFA emulsion is prepared by use of the same method, and the second immune injection is performed at the same dosage and by use of the same method; the experimental autoimmune cerebrospinal meningitis model established by use of the method has the clinical characters of remission-relapse, and can be widely used to the related field of disseminated sclerosis diseases. Compared with the existing rhesus method and model, the method and the model also have the characteristics of short induction period, low cost, rich monkey resources and the like, and also have the application value which cannot be realized by rodent models.

The invention discloses a non-therapeutic-purpose gene editing method based on PINK1 and PARK7 through injection of a virus into specific brain regions of animals. The virus is directly injected intothe specific brain regions of non-human primates by using a brain stereotaxic injection pump, wherein the injection volume is 1 [mu]L of the injected virus per 1 m<3>, and the titer of the injected virus is 10*10<13>vg / mL. Through the method, gene editing is performed directly on the specific brain regions of the non-human primates, and transgenic monkey models with disease phenotypes can be obtained in a short time, so that urgent research needs of Parkinson's syndromes (PD) at the present stage are met.

The invention relates to a positive electron medicine [18F] FPMMP as well as a preparation method and a midbody thereof. The positive electron medicine [18F] FPMMP has a following structure shown in the description. The positive electron medicine [18F] FPMMP can reach the peak value after 10 minutes after the injection to the brain part of the non-human primate animals; the clinic use is convenient; the BPND can reach 2.14 in a cingulate cortex.

The invention discloses a ureter ligation induced non-human primate animal renal fibrosis model building method and application. The specific technical scheme includes that an experimental monkey is anaesthetized by anesthetics, surgical anesthesia is maintained, the abdomen of the experimental monkey is cut apart along a medioventral line after disinfection, an abdominal cavity is opened, an operation field is exposed, a unilateral ureter at a unilateral renal lower edge is ligated twice by a surgical silk thread and completely blocked, another kidney is not ligated and serves as a self-body for comparison, a wound is stitched in a layered manner after a surgical incision is disinfected, renal fibrosis indexes are measured by biological samples at different time points after ligation, or a left kidney and a right kidney are taken down and fixed in neutral buffered formalin, and formalin fixed kidney samples are subjected to tissue section and pathological examination. An animal model has application values which cannot be realized by an existing rodent and rabbit animal model, and the non-human primate animal model built by the method has wide application in related fields of fibrosis.

The invention discloses a methyl CpG binding protein 2-based (MECP2-based) method for injecting a virus into a specific brain area of an animal without a therapeutic aim to perform gene editing. A cerebral stereotaxis injection pump is utilized to directly inject the virus into the specific brain area of a non-human primate; the injection volume is that 1 [mu]L of virus is injected per 1m<3>; andthe titer of the injected virus is 10*10<13> vg / mL. According to the method, the virus is directly injected into the specific brain area of the non-human primate to perform gene editing, and thus, a transgenic monkey model with the disease phenotype can be obtained in a short time, and the urgent research demand of an autism spectrum disorder (ASD) disease at the present stage is met.

Compositions and methods are provided for treating ocular disorders in a subject are provided. In one aspect, an adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding CNGA3. In another aspect, an adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding CNGB3. In another aspect, an adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding REP-1. In desired embodiments, the subject is human, cat, dog, sheep, or non-human primate.

The invention provides a method for building a non-human primate model with compulsive ritualistic behaviors. Aiming to overcome the technical defect that currently no typical ritualistic behavior animal model exists in the field, the invention discloses a method for inducing the ritualistic behaviors of an animal, and the animal model which has typical ritualistic behaviors and is evident in behavioral performance can be obtained in a short time by the method.

The invention discloses a method for injecting virus into a specific brain region of an animal for gene editing based on the non-treatment purpose of a PSEN1 gene. The method comprises the step of directly injecting virus into the specific brain region of a non-human primate by using a brain stereotactic injection pump, wherein the injection amount is 1 microliter of virus per 1m<3>, and the titerof the injected virus is 10*10<13> vg / mL. According to the method, virus is directly injected into the specific brain region of the non-human primate for gene editing, so that a transgenic monkey model with a disease phenotype can be obtained in a short time, and urgent research requirements of Alzheimer's disease (AD) at the present stage are met.

The invention discloses a method for measuring the thickness change of the crystalline lens of a non-human primate (monkey) through an A-type ultrasonic technology and verifying the relaxation effectof an M receptor antagonist on ciliary body smooth muscle. The method can be used for measuring the influence of test drugs with different concentrations on the thickness of the crystalline lens, knowing the dose-effect relationship between the test drugs and the thickness of the crystalline lens, speculating the influence of the drugs on refractive adjustment, detecting the effective dose and quickly and effectively guiding the preclinical research and development work of new drugs for preventing and treating juvenile myopia. The method disclosed by the invention is simple, stable in technology, good in repeatability, free of tissue trauma to animals and harmless to the health of the whole body. Experimental data obtained from monkeys (animals closest to human beings) has incomparable guiding significance to other animals in clinical tests, so that important transformation medical information is provided for research and development of innovative drugs.

The invention discloses a localization method of a non-human primate substantia nigra area. According to the localization method, localization coordinates of the non-human primate substantia nigra area are acquired via nuclear magnetic resonance pictures, so that accurate localization of the non-human primate substantia nigra area can be achieved. The method provided by the invent, in comparison with an existing extensive localization method which is implemented based on experiences, is more accurate and reliable and has the advantages of being efficient and being capable of saving experimental cost.

The invention discloses a construction method of a type 2 diabetes mellitus primate model, the method comprises the following steps that a middle-year experimental monkey is taken, high-glucose and high-fat feed is fed, the flameout time of a cage is 2: 00 to 12: 00, the normal flameout time is adjusted to be 20: 00 to 6: 00 of the next day after two weeks, the normal flameout time is adjusted to be 2: 00 to 12: 00 after two weeks, in this way, the flameout time is changed alternately, and after at least 6 months, molding is completed. According to the method, the method of disturbing animal circadian rhythm and eating time is used for the first time to force non-human primate experimental animals to change work and rest time, meanwhile, high-glucose and high-fat diet modeling is combined, the modeling efficiency is remarkably improved, and the modeling period is shortened by more than 50%; and the method is similar to natural pathogenesis, and the diabetes symptom is still maintained after induction is stopped half a year, which indicates that the model can be used as a stable T2DM model.

The present invention discloses a method for rapidly inducing a hepatic fibrosis animal model. The method includes: feeding a non-human primate for 120-160 d in a way of feeding the non-human primatewith a wine containing 10-35% ethanol according to an ethanol / body weight ratio of 4.0-5.7 g / kg every day, and meanwhile, freely feeding the non-human primate with a basal feed to obtain the hepatic fibrosis animal model. By using the method, a stable abiogenetic hepatic fibrosis animal model close to a human may be acquired, the non-human primate is better in compliance, inspection may be performed without operation or killing an animal in a test, the modeling time is short, and the modeling cost is low; and the method provides a good model carrier for research on pathogenesis of hepatic fibrosis, screening of drugs for treating hepatic fibrosis and pharmacodynamic evaluation.

The invention discloses a construction method of a rhesus monkey immunosuppression model and application of the rhesus monkey immunosuppression model in CAR-T cell implantation. 25mg / (kg / d) cyclophosphamide is combined with 2.5 mg / (kg / d) fludarabine to pre-treat rhesus monkeys, a disease model consistent with the change trend of various immune indexes under the human immunosuppression condition is established, and the reduction ranges of the white blood cell number (WBC) and the lymphocyte absolute number (LYM) in hematology exceed 50%; a flow cytometry detection result shows that CD3 is reduced by more than 40%, and the immunosuppression animal model is successfully built; the model is used for implanting CAR-T cells so as to be used for researching CAR-T immunotherapy. According to the method, the rhesus monkey immunosuppression animal model can be quickly and accurately established, sufficient immunosuppression can be ensured during CAR-T cell implantation of animals, so that CAR-T cells are alive in rhesus monkeys, and a more ideal and reliable non-human primate experimental animal model and a perfect evaluation system are provided for preclinical research of CAR-T immunotherapy.

The invention relates to the technical field of non-human primate experimental animal disease models, and discloses an establishment method and application of a machin type 2 diabetes model. The method comprises the following steps of (1) selecting male machin of which the age is 12-21 years old; (2) feeding the male machin for four times every day, specifically, feeding the male machin with sugar-containing feed for the first time, feeding the male machin with complementary food for the second time, feeding the male machin with green feed for the third time and feeding the the male machin with the sugar-containing feed for the fourth time; and (3) after feeding the male machin for one year according to the mode in the step (2), detecting corresponding indexes, and selecting the machin with the blood glucose level larger than 7 mmol / L in the fasting state, wherein the fed complementary food is brown sugar blocks and biscuits alternately, and the brown sugar blocks and the biscuits are alternated once every three days; and the sugar-containing feed is obtained by mixing conventional feed and brown sugar. According to the method, the machin type 2 diabetes model is established by adopting a warm feeding mode, and the machin type 2 diabetes model can be used for drug effect evaluation in the research and development process of new drugs.

The invention relates to a device for accurately measuring the water intake of a non-human primate experimental animal. The device comprises a water drinking device body and a recycling water tank, wherein a water tank is arranged at the upper part of the water drinking device body, and a water drinking pipe control valve assembly is arranged at the lower part of the water drinking device body; the water drinking pipe control valve assembly comprises a backflow type water outlet head, a backflow cover, a connecting tube, a valve control arm and a lifting type water valve; the lower end of the backflow type water outlet head communicates with the left end of the connecting tube, and the backflow type water outlet head is obliquely distributed upwards relative to the connecting tube; a connecting tube rotating shaft support is correspondingly arranged in the water drinking device body, rotating shafts are correspondingly arranged on the outer walls of the two sides of the connecting tube, and the connecting tube can be rotationally distributed on the connecting tube rotating shaft support through the rotating shafts; and one end of the lifting type water valve communicates with the lower end of the water tank through a water outlet pipeline, and the other end of the lifting type water valve communicates with the right end of the connecting tube through a water supply hose. The device has the advantages of being accurate in metering, high in anti-interference capacity, easy to domesticate, good in using effect and the like.

The invention discloses a test device for evaluation of a human or non-human primate animal space positioning ability. According to the technical key points, the test device comprises a supporting assembly (1), a wallboard assembly (2) and a folding door assembly (3), wherein the supporting assembly (1) comprises a floor assembly (14), a side column (12), a middle column (13) and a guide rail assembly (11), the side column (12) and the middle column (13) are perpendicular to the floor assembly (14), and the guide rail assembly (11) is supported on a ceiling; the wallboard assembly (2) comprises a plurality of U-shaped trolley shells, walking wheels (313) arranged between the trolley shells and strip-shaped wallboards arranged at the bottoms of the trolley shells; the folding door assembly(3) comprises a sliding trolley assembly (31), a telescopic upright column assembly (32) and a foldable door panel assembly (33); and the foldable door panel assembly (33) comprises a plurality of hinged parts (333) fixed to hoops and a plurality of door panel assemblies (33) matched with a telescopic upright column sleeve pipe and connected end to end in sequence. The test device has the advantages of various functions, reliable and effective evaluation result, convenient use, low use cost and the like.