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Feed for constructing nonalcoholic steatohepatitis model of nonhuman primates and application method of feed

A primate, steatohepatitis technology, applied in the field of biomedicine, can solve the problem that terminal patients can only seek liver transplantation

Pending Publication Date: 2020-06-26
KUNMING BIOMED INT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gaps in drug treatment for the disease leave advanced patients seeking liver transplants

Method used

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  • Feed for constructing nonalcoholic steatohepatitis model of nonhuman primates and application method of feed
  • Feed for constructing nonalcoholic steatohepatitis model of nonhuman primates and application method of feed
  • Feed for constructing nonalcoholic steatohepatitis model of nonhuman primates and application method of feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] 1) Screening of non-human primates: select animals, select the number of cultures, the weight of the animals is ≥7.5kg, the age is ≥10 years old, the liver function of the animals is normal, and there is no feeding history of other inducers;

[0073] 2) Feed preparation: with the soybean meal of 22% weight portion, the Peruvian fishmeal of 2.5% weight portion, the feed grade calcium hydrogen phosphate of 2% weight portion, the salt of 0.2% weight portion, the compound multivitamin of 0.1% weight portion, 0.1 % weight portion of high-stable VC, 0.4% weight portion of cholesterol, 20% weight portion of monocrystalline fructose, 15% weight portion of animal fat, 0.2% weight portion of methionine, 1% weight portion of premixed minerals and 30 The first grade flour of % weight part is stirred and mixed, obtains the feed for constructing non-alcoholic steatohepatitis model of non-human primate;

[0074] 3) Cultivation: Raise the qualified animals in a single cage indoors, wit...

Embodiment 2

[0092]1) Screening of non-human primates: select animals, select the number of cultures, the weight of the animals is ≥7.5kg, the age is ≥10 years old, the liver function of the animals is normal, and there is no feeding history of other inducers;

[0093] 2) Feed preparation: with the soybean meal of 28% weight portion, the Peruvian fishmeal of 6% weight portion, the feed grade dibasic calcium phosphate of 3% weight portion, the salt of 0.4% weight portion, the compound multivitamin of 0.3% weight portion, 0.3 % weight portion of high-stable VC, 0.6% weight portion of cholesterol, 25% weight portion of monocrystalline fructose, 20% weight portion of animal fat, 0.4% weight portion of methionine, 2% weight portion of premixed minerals and 35 The first grade flour of % weight part is stirred and mixed, obtains the feed for constructing non-alcoholic steatohepatitis model of non-human primate;

[0094] 3) Cultivation: Raise the qualified animals in a single cage indoors, with an...

Embodiment 3

[0112] HE-stained sections are used for NAS (NAFLD Activity Score non-alcoholic fatty liver disease scoring), standard animal model HE-stained sections see figure 1 and figure 2 , figure 2 Medium histopathological lesions: steatosis (3 points), balloon-like lesions (2 points), hepatic lobular inflammation (3 points), NAS composite score 8 points, the top arrow in the figure indicates balloon-like lesions, and the middle arrow in the figure indicates Inflammation of the hepatic lobules, and fatty degeneration indicated by the arrow at the bottom of the figure;

[0113] Feed preparation: the soybean meal of 25% by weight, the Peruvian fishmeal of 3% by weight, the feed grade dibasic calcium phosphate of 2.5% by weight, the salt of 0.3% by weight, the compound multivitamins of 0.2% by weight, 0.2% by weight The high-stable VC of 0.5%, the cholesterol of 0.5% by weight, the monocrystalline fructose of 20% by weight, the tallow of 15% by weight, the methionine of 0.3% by weight...

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Abstract

The invention discloses a feed for constructing a nonalcoholic steatohepatitis model of nonhuman primates and an application method of the feed. The feed comprises the following raw materials in partsby weight of 22-28% of soybean meal, 2.5-3.5% of Peru fish meal, 2-3% of feed-grade calcium hydrogen phosphate, 0.2-0.4% of salt, 0.1-0.3% of composite multiplex vitamins, 0.1-0.3% of high-stabilityVC, 0.4-0.6% of cholesterol, 20-25% of monocrystal levulose, 15-20% of animal oil and fat, 0.2-0.4% of methionine, 1-2% of premix minerals, and 30-35% of first-grade flour. A nonalcoholic steatohepatitis disease scoring system standard is used, and various individual index scores in a fibrosis scoring standard are obviously increased. After the feed is used for feeding the nonhuman primates for 3months, nonalcoholic steatohepatitis liver injury can be established, so that the nonalcoholic steatohepatitis animal model is formed.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a feed for constructing a non-alcoholic steatohepatitis model of non-human primates and a use method thereof. Background technique [0002] As a country with a high incidence of liver disease in China, patients with liver disease have caused serious economic and social burdens to the country and society. Data show that in 2016, the overall scale of drugs for liver diseases in my country exceeded 50 billion yuan. Nonalcoholic steatohepatitis (NASH, nonalcoholic steatohepatitis) is an extreme form of nonalcoholic fatty liver, defined as the appearance of steatosis accompanied by inflammation and liver cell damage. NASH can lead to advanced liver fibrosis, cirrhosis, liver failure and the development of liver tumors. In the past 20 years, the incidence of NAFLD has doubled and is now the most common liver disease in China and Western countries. At present, there is no drug to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/00A23K10/37A23K10/22A23K10/30A23K20/26A23K20/22A23K20/174A23K20/163A23K20/158A23K20/142A23K20/20A23K20/10A01K67/02
CPCA23K50/00A23K10/37A23K10/22A23K10/30A23K20/26A23K20/22A23K20/174A23K20/163A23K20/158A23K20/142A23K20/30A23K20/10A01K67/02A01K2227/106A01K2267/0368Y02P60/87
Inventor 张龙李绍东李锋张宝弘
Owner KUNMING BIOMED INT
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