77 results about "ORDER PRIMATES" patented technology

Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from the group consisting of feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from the group consisting of nucleosides and a pyruvate salt.

The present invention relates to the elucidation that TRPML3 is involved in salty taste perception in primates including humans and likely other mammals and based thereon high-throughput mammalian and medium-throughput oocyte-based electrophysiological assays for identifying human TRPML3 modulators, preferably TRPML3 enhancers. Compounds that modulate TRPML3 function in the assay are expected to affect salty taste in humans. The inventive electrophysiological assays, such as the two-electrode voltage-clamp technique, facilitate the identification of compounds which specifically modulate human TRPML3. The assays of the invention provide a robust screen useful to detect compounds that facilitate (enhance) or inhibit TRPML3 function. Compounds that enhance or block TRPML3 channel activity should thereby modulate salty taste. In addition, these compounds may be used to regulate sodium excretion, urinary output and other biological functions relating to sodium levels and TRPML3 related functions.

In certain aspects, the present invention provides compositions and methods for increasing red blood cell and / or hemoglobin levels in vertebrates, including rodents and primates, and particularly in humans.

The present invention provides compositions and methods of treatment of diseases that are sensitive to drugs that downregulate the function of microRNA's, mRNA, non-coding RNA, or viral genomes. In particular, it has been discovered that a very long term effect of an anti microRNA oligonucleotide may be obtained when administered to a primate. Therefore, the present invention relate to pharmaceutical compositions and methods for treatment of primates, including humans wherein the compositions are administered with a long time interval.

Oligonucleotides containing the non-palindromic sequence motif:X1X2X3X4X5X6X7X8,wherein X1 is C,T,G or A (preferably T or C); wherein X2 is C,T,G or A; wherein X7 is C,T,G or A (preferably G); at least three, and preferably all, of X3, X4, X5, X6 and X8 are T; and with the proviso that, in the motif, a C does not precede a G (in other terms, the nucleic acid motif does not consist of a CpG oligonucleotide), that modulate the immune response of animals of the order Primate, including humans, are disclosed. This immune modulation is characterized by stimulation of proliferation, differentiation, cytokine production and antibody production on B-cells and cell differentiation on plasmacytoid dendritic cells.

Methods for obtaining pluripotent (embryonic stem) cells from parthenogenetic embryos, especially primates, are provided. These cells are useful for producing differentiated cells, tissues and organs, especially human and non-human primate cells, tissues and organs.

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins in mammalian host cells, for example, primate host cells and rodent host cells. The invention provides, for example but not limited to, translation systems that include host cells (e.g., primate or rodent cells), orthogonal aminoacyl-tRNA synthetases derived from eubacterial synthetases, orthogonal tRNAs, and the unnatural amino acid. The invention also relates to methods for producing proteins of interest comprising at least one unnatural amino acid in mammalian host cell systems.

Disclosed are constructs and methods for expressing DNAs of interest in particular in non- primate eukaryotic host cells that display advantages with regard quantity and quality of expression including high stability of expression and, if appropriate, transport of the expression product out of the cell.

The present invention relates to pharmaceutical or veterinary or nutritional compositions of polyherbal extracts useful as anti-viral or immune-supporting agents. Particularly, the present invention of polyherbal composition comprises of extracts of Withania somnifera, Mangifera indica and purified Shilajit. This cost effective immune-supporting agent is ideal for use during the maintenance phase of the treatment, following an initial viral load reduction phase in which it is used as an adjuvant to conventional anti-viral drug therapy. The anti-viral and immune-supporting composition of this invention can perhaps be the sole basis of treatment where affordability is an issue. Additionally, this composition is used for the treatment, prevention or management of immune-supporting system in primates in need, especially humans.

The invention provides a diabetes primate model construction method. The method comprises the following steps of injecting streptozotocin into cynomolgus monkeys according to a small-dose progressiveincrease method, feeding the cynomolgus monkeys with high-sugar and high-fat feed at the same time, and after injecting the streptozotocin into the cynomolgus monkeys and feeding the cynomolgus monkeys with the high-sugar and high-fat feed for a period of time, increasing night illumination to induce the cynomolgus monkeys to become diabetes models. The diabetic monkey model is successfully established by adopting the method for 1.5-6 months, the model has the core characteristics of insulin resistance and hyperglycemia, the duration of hyperglycemia is long, and the hyperglycemia is not reduced after more than 200 days. The animal model established by the invention can be used in animal experiments with longer duration, and can be sold as a mature animal model to generate economic benefits.

Oligonucleotides containing the non-palindromic sequence motif: X1X2X3X4X5X6X7X8, wherein X1 is C,T,G or A (preferably T or C); wherein X2 is C,T,G or A; wherein X7 is C,T,G or A (preferably G); at least three, and preferably all, of X3, X4, X5, X6 and X8 are T; and with the proviso that, in the motif, a C does not precede a G (in other terms, the nucleic acid motif does not consist of a CpG oligonucleotide), that modulate the immune response of animals of the order Primate, including humans, are disclosed. This immune modulation is characterized by stimulation of proliferation, differentiation, cytokine production and antibody production on B-cells and cell differentiation on plasmacytoid dendritic cells.

The present invention relates to high-throughput mammalian and medium-throughput oocyte-based electrophysiological assays for identifying human TRPML3 modulators, preferably TRPML3 enhancers. Compounds that modulate TRPML3 function in the assay are expected to affect salty taste in humans. The inventive electrophysiological assays, such as the two-electrode voltage-clamp technique, facilitate the identification of compounds which specifically modulate human TRPML3. The assays of the invention provide a robust screen useful to detect compounds that facilitate (enhance) or inhibit TRPML3 function. Compounds that enhance or block TRPML3 channel activity should thereby modulate salty taste. In addition, these compounds may be used to regulate sodium excretion, urinary output and other biological functions relating to sodium levels. This invention relates to the elucidation that TRPML3 is involved in salty taste perception in primates including humans and likely other mammals (given the significance of sodium and other ions to physiological functions and conditions this phenotype is likely strongly conserved in different animals). The TRPML3 gene also modulates one or more of sodium metabolism, sodium excretion, blood pressure, fluid retention, cardiac function and urinary functions such as urine production and excretion. The inventors have identified TRPML3 as encoding a salty taste receptor in primates and humans (and likely other mammals) based on gene expression assays which have determined that TRPML3 is expressed specifically in taste bud cells and not in lingual epithelial cells, similar assays that have determined that TRPML3 is specifically expressed or enriched in the top half of taste bud cells in a subset of taste cells which do not express TRPM5 or PKD2L1, prior reports that document the expression of TRPML3 in other sensory organs such as the ear (therefore further substantiating the role of TRPML3 as a 'professional' sensory gene).

A construction method of a primate liver cancer model comprises the following steps: a) inserting gRNA sequence for specifically targeting miR122 into an appropriate vector to construct a template vector for in vitro transcription of miR122; b) inserting cas9 gene sequence into an appropriate vector to construct a template vector for in vitro transcription of cas9 gene; c) carrying out PCR amplification by using the vector in the Step a) so as to obtain a gRNA temperate for in vitro transcription of miR122; d) linearizing the template vector in the Step b) by the use of restriction enzyme so as to obtain a template for in vitro transcription of cas9 gene; e) carrying out in vitro transcription by using products in the Step c) and Step d) as templates so as to obtain gRNA for specifically targeting miR122 and mRNA of cas9 gene; f) injecting the gRNA and mRNA in the Step e) into fertilized egg cell of the animal so as to generate stable miR122-knockout animal offspring.

The invention relates to the field of molecular biology, in particular to a primate animal DNA methylation relative quantification kit. The kit comprises: a) a juvenile zebrafish DNA or zebrafish DNA without CpG site, and b) a DNA quantitative detection reagent which at least comprises a reagent for quantitative detection of a), wherein the juvenile zebrafish is zebrafish from fertilized egg formation to development within 120 hours. According to the kit, accurate quantification of DNA methylation of primates can be realized, and the problem that endogenous internal reference is prone to instability or genome DNA pollution caused by own factors or sample collection reasons is solved.

The invention discloses a non-human primate behavioral data acquisition device and a data acquisition method.The non-human primate behavioral data acquisition device comprises an animal movement device, a plurality of stereo cameras and an analysis terminal, wherein the animal movement device comprises a running machine, a hanging bracket, an animal strait coat and a plurality of fluorescent paints; the hanging bracket is erected above a running belt of the running machine, a hanging rod extending towards the running belt is arranged on the hanging bracket, a first ring buckle is arranged at the tail end of the hanging rod, a second ring buckle is arranged on the animal strait coat, and the first ring buckle and the second ring buckle are correspondingly buckled. The plurality of fluorescent paints are used for marking the joints; the number of the stereo cameras is not less than four, and the stereo cameras are arranged on the peripheral area of the running machine at intervals and used for collecting animal gait data and transmitting the data to the analysis terminal. The non-human primate behavioral gait data can be effectively collected, and the non-human primate behavioral gait data can be analyzed.