Refrigeration preservation and resuscitation method of sperm of non-human primates
A primate and cryopreservation technology, which is applied to the field of sperm cryopreservation and recovery of non-human primates, can solve problems such as poor recovery rate, and achieve a simple cryopreservation and recovery method, wide applicability, and price reduction. The effect of freezing costs
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[0019] Experimental animals: 6 healthy, fertile male cynomolgus monkeys and rhesus monkeys (3 each, aged 7-10 years) were from the Drug Safety Evaluation Research Center of the Institute of Medical Biology, Chinese Academy of Medical Sciences. Each monkey was raised in a single cage, fed with 3 meals a day, and given a certain amount of clean fresh fruit every day as supplementary food. Keep 12h / 12h light and dark every day, at room temperature of 17-22°C. The Drug Safety Evaluation Research Center of the Institute of Medical Biology, Chinese Academy of Medical Sciences has obtained the license for the use of experimental animals from the Kunming Science and Technology Bureau. The experimental protocol has been reviewed and approved by the Ethics Review Committee of the Institute of Medical Biology, Chinese Academy of Medical Sciences before implementation. The use of animals complies with the 3R (Reduction, Replacement, Refinement) principle. All operations related to exper...
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