Construction method of type 2 diabetes primate model

Abstract

The invention discloses a construction method of a type 2 diabetes mellitus primate model, the method comprises the following steps that a middle-year experimental monkey is taken, high-glucose and high-fat feed is fed, the flameout time of a cage is 2: 00 to 12: 00, the normal flameout time is adjusted to be 20: 00 to 6: 00 of the next day after two weeks, the normal flameout time is adjusted to be 2: 00 to 12: 00 after two weeks, in this way, the flameout time is changed alternately, and after at least 6 months, molding is completed. According to the method, the method of disturbing animal circadian rhythm and eating time is used for the first time to force non-human primate experimental animals to change work and rest time, meanwhile, high-glucose and high-fat diet modeling is combined, the modeling efficiency is remarkably improved, and the modeling period is shortened by more than 50%; and the method is similar to natural pathogenesis, and the diabetes symptom is still maintained after induction is stopped half a year, which indicates that the model can be used as a stable T2DM model.

Description

technical field [0001] The invention relates to a method for constructing an animal model, in particular to a method for constructing a type 2 diabetes primate model. Background technique [0002] At present, primate models of Type II diabetes mellitus (T2DM) mainly rely on natural selection, high-energy diet induction and drug induction. However, the number of model animals obtained by natural screening is limited, the cycle of dietary induction is long and the efficiency is not high, the pathological mechanism of drug induction is different from clinical, and the scope of application is narrow. [0003] The currently reported primate (monkey) diabetes models mainly include high-sugar and high-fat diet induction (CN103478071A), light induction (CN108812525A), and combined injection of chemical drug streptozotocin (STZ) induction (CN 112425561A), etc. Way. [0004] The induction period of simple high-sugar and high-fat diet is relatively long, requiring 2-4 years, and the ...

AI Technical Summary

Problems solved by technology

[0004] Simple high-sugar and high-fat diet induction cycle is relatively long, it takes 2-4 years, and the molding efficiency is relatively low (less than 5%)

Its model also does not appear signs closely related to type 2 diabetes, such as obesity and abnormal sugar, fat, and cholesterol metabolism, which also shows that its model is not a good type 2 diabetes model

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