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Construction method of type 2 diabetes primate model

A primate and construction method technology, applied in the field of construction of a type 2 diabetes primate model, can solve the problems of low modeling efficiency, long induction period of high-sugar and high-fat diet, not being a type 2 diabetes model, etc. achieve obvious effect

Inactive Publication Date: 2021-12-03
广州伊纳维森生物科技有限公司
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AI Technical Summary

Problems solved by technology

[0004] Simple high-sugar and high-fat diet induction cycle is relatively long, it takes 2-4 years, and the molding efficiency is relatively low (less than 5%)
Its model also does not appear signs closely related to type 2 diabetes, such as obesity and abnormal sugar, fat, and cholesterol metabolism, which also shows that its model is not a good type 2 diabetes model

Method used

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  • Construction method of type 2 diabetes primate model
  • Construction method of type 2 diabetes primate model
  • Construction method of type 2 diabetes primate model

Examples

Experimental program
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Effect test

Embodiment

[0022] A method for constructing a type 2 diabetes primate model, comprising the following steps:

[0023] Randomly select a group of 14-18-year-old experimental monkeys (cynomolgus cynomolgus, Macaca fascicularis, purchased from Guangxi Guidong Primate Development Experiment Co., Ltd.), and eliminate some individuals who may belong to spontaneous diabetes by measuring fasting blood glucose (FPG≥ 4.5). 90 animals with normal fasting blood glucose were left and randomly divided into 3 groups. The first group was the normal control group, and the lights were turned off from 20:00 to 6:00 the next day; From 20:00 to 6:00 the next day; the third group (experimental group) was given high-sugar and high-fat feed and changed the light-off time of the feeding room. The specific method was that the light-off time of the cage was 2:00-12:00. After one week, it will be adjusted to the normal light-off time from 20:00 to 6:00 the next day, and after another two weeks, it will be adjusted...

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Abstract

The invention discloses a construction method of a type 2 diabetes mellitus primate model, the method comprises the following steps that a middle-year experimental monkey is taken, high-glucose and high-fat feed is fed, the flameout time of a cage is 2: 00 to 12: 00, the normal flameout time is adjusted to be 20: 00 to 6: 00 of the next day after two weeks, the normal flameout time is adjusted to be 2: 00 to 12: 00 after two weeks, in this way, the flameout time is changed alternately, and after at least 6 months, molding is completed. According to the method, the method of disturbing animal circadian rhythm and eating time is used for the first time to force non-human primate experimental animals to change work and rest time, meanwhile, high-glucose and high-fat diet modeling is combined, the modeling efficiency is remarkably improved, and the modeling period is shortened by more than 50%; and the method is similar to natural pathogenesis, and the diabetes symptom is still maintained after induction is stopped half a year, which indicates that the model can be used as a stable T2DM model.

Description

technical field [0001] The invention relates to a method for constructing an animal model, in particular to a method for constructing a type 2 diabetes primate model. Background technique [0002] At present, primate models of Type II diabetes mellitus (T2DM) mainly rely on natural selection, high-energy diet induction and drug induction. However, the number of model animals obtained by natural screening is limited, the cycle of dietary induction is long and the efficiency is not high, the pathological mechanism of drug induction is different from clinical, and the scope of application is narrow. [0003] The currently reported primate (monkey) diabetes models mainly include high-sugar and high-fat diet induction (CN103478071A), light induction (CN108812525A), and combined injection of chemical drug streptozotocin (STZ) induction (CN 112425561A), etc. Way. [0004] The induction period of simple high-sugar and high-fat diet is relatively long, requiring 2-4 years, and the ...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K2207/25A01K2207/35A01K2227/106A01K2267/0362
Inventor 郭祥玉陈若虹徐强
Owner 广州伊纳维森生物科技有限公司
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