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Method for rapidly inducing hepatic fibrosis animal model

A liver fibrosis and animal model technology, applied in the direction of animal feed, additional food elements, applications, etc., can solve the problems of complicated operation, high experimental technical requirements, and increased modeling cost due to the degree of liver fibrosis, so as to speed up the molding speed. , Reduce the cost of modeling, the effect of model stability

Active Publication Date: 2020-09-08
GUANGXI UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the degree of liver fibrosis is relatively mild, and the requirements for experimental techniques are relatively high, and the operation is cumbersome. Further improvement is needed for popularization and application to aggravate the degree of liver fibrosis and reduce the cost of modeling.
[0004] Although there are many animal models of liver fibrosis, due to the complex and diverse causes of liver fibrosis and the differences between animal and human species, so far, animal models that can fully reflect human liver fibrosis have not been completely successful. In the work, fully consider the etiology and mechanism of liver fibrosis, the species differences between humans and animals, and the natural recovery rate of model animals, etc., to select the most suitable animal model

Method used

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  • Method for rapidly inducing hepatic fibrosis animal model
  • Method for rapidly inducing hepatic fibrosis animal model
  • Method for rapidly inducing hepatic fibrosis animal model

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Experimental program
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preparation example Construction

[0057] (2) Basic feed. The basic feed is a common feed for daily feeding of commercially available non-human primates. The nutritive ingredient contains the following weight percentages: 18.3% of crude protein, 5.6% of crude fat, 3.5% of crude fiber, 3.8% of crude ash, 1.2% of calcium and 1.0% of phosphorus. The preparation method of the basic feed comprises the following steps: 1. Take rice, soybeans, and soybean meal, pulverize them respectively, and pass through a 60-mesh sieve to obtain crushed rice flour, soybean meal, and soybean meal powder; 2. Take 0.1kg of vitamin E, Soybean oil 8kg, mix well, add flour according to the method of increasing in equal amount, mix well, pass through a 60-mesh sieve to get flour mixture A evenly dispersed; 3. Take choline chloride 1.5kg, vitamin C 0.2kg, calcium pantothenate 0.15kg, Mix well, add 3.6kg of stone powder, mix well, then add 3.7kg of salt, 12kg of calcium hydrogen phosphate, mix well, and get mixture B; 4. Take 45kg of soybe...

Embodiment 2

[0107] B ultrasonic observation after embodiment 2 feeding 120 days, see Figure 11 . The liver is smaller than before, the liver capsule is thickened, the surface of the liver is uneven and jagged, the edge of the liver becomes blunt and becomes irregular, the echo of the distal liver parenchyma is diffusely increased, and the echo spots are dense and different in size, appearing as dots , suggesting liver fibrosis.

[0108] See Figure 12 , is the HE staining diagram of the hepatic tissue structure of the rhesus monkey fed for 120 days in Example 2. Before the experiment, the hepatic lobule was clearly outlined, the hepatic cells were arranged radially around the central lobular vein, the cords of the hepatic cells were arranged neatly, the hepatic cells were polygonal, the cell boundary was clear, the nucleus was round, located in the center of the cell, the cytoplasm was rich and basophilic Occasionally, cytoplasm of hepatocytes around the central vein is loose, and und...

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Abstract

The present invention discloses a method for rapidly inducing a hepatic fibrosis animal model. The method includes: feeding a non-human primate for 120-160 d in a way of feeding the non-human primatewith a wine containing 10-35% ethanol according to an ethanol / body weight ratio of 4.0-5.7 g / kg every day, and meanwhile, freely feeding the non-human primate with a basal feed to obtain the hepatic fibrosis animal model. By using the method, a stable abiogenetic hepatic fibrosis animal model close to a human may be acquired, the non-human primate is better in compliance, inspection may be performed without operation or killing an animal in a test, the modeling time is short, and the modeling cost is low; and the method provides a good model carrier for research on pathogenesis of hepatic fibrosis, screening of drugs for treating hepatic fibrosis and pharmacodynamic evaluation.

Description

technical field [0001] The invention relates to a method for establishing an animal disease model, in particular to a method for rapidly inducing an animal model of liver fibrosis. Background technique [0002] Hepatic fibrosis (hepatic fibrosis, HF) is a common manifestation of many chronic liver diseases such as viral hepatitis and alcoholic liver disease. Pathophysiological process of deposition. If the liver damage continues to develop, mild liver fibrosis will develop into decompensated cirrhosis. As far as the current medical level is concerned, there is no good treatment for cirrhosis. The survival rate is only 14%. At the same time, the continuous development of liver fibrosis may also lead to abnormal liver function and even lead to hepatocellular carcinoma, which greatly threatens human life and health. Therefore, the construction of an ideal animal model that can simulate the process of human liver fibrosis has become the key to exploring early diagnosis, findin...

Claims

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Application Information

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IPC IPC(8): A01K67/02A23K20/105
CPCA01K67/02A23K20/105
Inventor 冷静梁丹唐海波吴展帅李晓红肖健唐耀平
Owner GUANGXI UNIV OF CHINESE MEDICINE
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