The invention belongs to a collaurum immunochromatography test strip detecting mycoplasma pneumoniae, comprising a sample pad, a combining pad, a nitrocellulose coating film, a water absorption pad and a PVC bottom plate; the sample pad, the combining pad, the nitrocellulose coating film and the water absorption pad are overlapped on the PVC bottom plate in sequence; the combining pad is coated with a mycoplasma pneumoniae antibody MPh2-collaurum-carbon nanotube marker; the nitrocellulose coating film is provided with a detection line and a quality control line; the detection line is coated with a mycoplasma pneumoniae antibody MPh1 and the quality control line is coated with a goat anti mouse IgG. The invention further comprises a preparing method of the collaurum immunochromatography test strip, comprising the steps of preparing of collaurum, preparing of a gold marker antibody, purifying and assembling of the test strip. The collaurum immunochromatography test strip has the characteristics of high specificity, high sensitivity, simple and convenient operation, fast detection, accuracy and suitability for field use.