36 results about "Mycoplasma antigen" patented technology

The invention provides a preparation method of a chicken infectious rhinitis and mycoplasma gallisepticum bivalent lipid inactivated vaccine, belonging to the technical field of biological products for animals. The preparation method is implemented by the following steps of: preparing a type A haemophilus paragallinarum inactivated antigen bacterial liquid, a type C haemophilus paragallinarum inactivated antigen bacterial liquid and a mycoplasma gallisepticum antigen liquid; uniformly mixing the type A haemophilus paragallinarum inactivated antigen bacterial liquid with the type C haemophilus paragallinarum inactivated antigen bacterial liquid in the volume ratio of 1:1 to obtain a mixed haemophilus paragallinarum inactivated antigen bacterial liquid; uniformly mixing the mixed haemophilus paragallinarum inactivated antigen bacterial liquid with the mycoplasma gallisepticum antigen liquid in the ratio of 1:1-1:1.5, pouring into an emulsifying tank and stirring; slowly adding poplar bark lipid till the final volume percentage concentration of the poplar bark lipid in the mixture is 2.0-3.8 percent; and continually stirring and emulsifying for 30-60 minutes. The vaccine has the advantages of easiness for absorbing, no toxic or side effect, short immunogenic time, long immune duration, good immune effect and capability of effectively preventing chicken infectious rhinitis and mycoplasma gallisepticum.

The invention relates to a mycoplasma pneumoniae IgM antibody colloid gold method detecting kit which comprises a nitrocellulose membrane detecting line coated recombinant antigen MP-Ag, a quality control line coated goat anti-mouse IgG antibody and a gold labeled pad coated mouse anti-human IgM monoclonal antibody which is marked by colloidal gold, wherein the recombinant mycoplasma pneumoniae antigen is colorless transparent liquid, has the concentration of larger than 2mg/ml and is detected by SDS-PAG; the goat anti-mouse IgG antibody is colorless transparent liquid and has the concentration of larger than 4mg/ml; the mouse anti-human IgM monoclonal antibody is colorless transparent liquid, has the concentration of larger than 2mg/ml and is detected by SDS-PAGE; and the sample loading amount uses two strips under the condition of 10 microliters. The mycoplasma pneumoniae IgM antibody colloid gold rapid detecting test paper tape uses multiepitope recombinant antigen as a raw materials, has the characteristics of simpler operation, low cost, high specificity and high sensitivity, can carry out single detection, is easy for popularization and has obvious detecting and control effect on the mycoplasma pneumoniae IgM antibody.

The invention relates to the field of in-vitro diagnostic immunoassay, and in particular provides a mycoplasma pneumoniae antigen as well as a preparation method and application thereof. The mycoplasma pneumoniae antigen provided by the invention is protein composed of an antigenic protein sequence P1M:residues 1340-1518, specifically an amino acid sequence shown in SEQ ID NO.1, or protein which is obtained by performing substitution and/or deletion and/or addition on the amino acid sequence shown in the SEQ ID NO.1 by one or more amino acid residues and has same function. The antigen is verified by an immunoserological detection technology to have strong specificity and high sensitivity, and the antigen is easy to culture and purify, is more conducive to industrial production and saves costs; and in addition, the antigen is suitable for preparation of MP antibody detection products, can be used for any forms of products in the field of in-vitro immunodiagnosis, and has broad market prospects.

The invention provides application of a mycoplasma hyopneumoniae antigen or an immunogenic composition containing the antigen in prevention and treatment of PRDC (porcine respiratory disease complex) and diseases with PRDC relevant clinical evidence. Specifically, the application includes immunization of pigs with an immune dosage of the mycoplasma hyopneumoniae antigen or the immunogenic composition containing the mycoplasma hyopneumoniae antigen. Vaccination with vaccines containing the mycoplasma hyopneumoniae antigen on animals can significantly alleviate and reduce the severity and duration of PRDC relevant clinical evidence.

The invention relates to the field of biological reagents, in particular to coating liquid for improving the stability of chlamydia pneumoniae antigen/mycoplasma antigen in an immunochromatographic reagent and a preparation method thereof. The coating solution comprises a buffer solution, wherein the buffer solution comprises a first component for immobilizing a chlamydia antigen or a mycoplasma antigen on a nitrocellulose membrane, a stabilizer, an antioxidant and a preservative. The first component is beneficial to immobilization of the antigen on the nitrocellulose membrane. The stabilizercan improve the stability of the mycoplasma antigen and the chlamydia antigen. The antioxidant can prevent the mycoplasma antigen and the chlamydia antigen from being oxidized. The preservative can play a role in preventing mycoplasma antigens and chlamydia antigens from being corroded. All the components in the coating liquid cooperate with one another to protect the stability of the antigen in the immunochromatographic reagent.

The invention discloses a Mycoplasma pneumoniae antigen and its application in simultaneous quantitative detection of Mycoplasma pneumoniae IgG and IgM content in peripheral blood. The amino acid sequence of the mutant Mycoplasma pneumoniae antigen P80 of the invention is as shown in SEQ ID NO.1. The immunoreactivity of the Mycoplasma pneumoniae antigen p80 and the antibody is about 34.6% higher than that of the natural antigen. By combining the Mycoplasma pneumoniae antigen P80 with the quantum dot immunochromatography technology, the shortcoming that existing detection methods of Mycoplasmapneumoniae antibody, such as immunochromatography assay (ICA) and enzyme-linked immunosorbent assay (ELISA), can only detect qualitatively or semi-quantitatively, and the MP-IgM and MP-IgG detection must be carried out twice is solved; and the detection of MP-IgM and MP-IgG can be realized at the same time, so as to distinguish the patients with recent infection or previous infection and reduce the misdiagnosis rate.

The invention belongs to the field of medical detection equipment, and provides a combined device for synchronously detecting IgM antibodies of influenza A and B viruses and chlamydia pneumoniae and mycoplasma pneumoniae. The device comprises a double-channel clamping shell, a test strip FluA & B and a test strip CP & MP which are arranged in the double-channel clamping shell in parallel; influenza virus A detection lines arranged on a nitrocellulose membrane of the test strip FluA & B at intervals are coated with high-specificity influenza virus A antigens, influenza virus B detection linesare coated with high-specificity influenza virus B antigens, and first quality control lines are coated with quality control line coating receptors; chlamydia pneumoniae detection lines (CP) arranged on a nitrocellulose membrane of the test strip CP & MP at intervals are coated with high-specificity chlamydia pneumoniae antigens, mycoplasma pneumoniae detection lines (MP) are coated with high-specificity mycoplasma pneumoniae antigens, and second quality control lines are coated with quality control line coating receptors.

The invention provides a preparation method of serum-free mycoplasma ovipneumoniae antigen. The method comprises the following steps: (1) preparation of chicken embryos: preparing the healthy SPF chicken embryos at 7-8 days of age, the embryos are vigorous, clear in blood vessels, and cultured in an incubator; (2) preparation of mycoplasma ovipneumoniae inoculum; and (3) chicken embryo inoculation, culture and harvest: inoculating the healthy SPF chicken embryos of 7-8 days old in step (1) of the mycoplasma ovipneumoniae inoculum, 0.2ml of the inoculum is inoculated for each chicken embryo, After inoculation, the incubator is further cultured for 5 days, and the allantoic fluid of dead chicken embryos and/or the allantoic fluid of chicken embryos that do not died after 5 days are aseptically obtained to obtain the serum-free mycoplasma ovipneumoniae antigen. In the present invention, the mycoplasma ovipneumoniae is subcultured in chicken embryos, and the allantoic fluid of chicken embryos is collected to obtain the serum-free mycoplasma ovipneumoniae antigen. The method of the present invention does not use serum, and can avoid many problems in using serum.

The invention discloses a mycoplasma bovis P48 gene. The base sequence of the mycoplasma bovis P48 gene is shown as SEQ ID NO. 1 in a sequence table; a mycoplasma bovis P48 antigen protein is a protein expressed by the mycoplasma bovis P48 gene; an anti-mycoplasma bovis polyclonal antibody is a polyclonal antibody prepared by taking the mycoplasma bovis P48 antigen protein as an antigen; and a preparation method of an anti-mycoplasma bovis immunomagnetic bead is disclosed. Results show that 1) the coupling efficiency of NHS magnetic microspheres is highest when a coupling buffer solution is 2-morpholineethanesulfonic acid (MES) and the antibody concentration is 200 [mu] g/mL; and 2) the prepared immunomagnetic beads can be enriched into mycoplasma bovis within 15 minutes, the lowest enrichment concentrations are that the bacterium concentration is 10<-3>CCU/mL, and the immunomagnetic beads have good specificity and sensitivity.

The invention discloses a porcine circovirus, porcine pseudorabies virus and mycoplasma triple inactivated vaccine which comprises an antigen and a vaccine adjuvant, the antigen is composed of a porcine circovirus type 2 antigen, a porcine pseudorabies virus antigen and a mycoplasma antigen, the porcine circovirus type 2 antigen is a purified, concentrated and inactivated porcine circovirus type 2 protein antigen solution, and the content of Cap protein is more than or equal to 160 [mu]g/ml; the porcine pseudorabies virus antigen is a purified, concentrated and inactivated porcine pseudorabies virus protein antigen solution, and the content of the Cap protein is more than or equal to 160 [mu]g/ml; the mycoplasma antigen is an inactivated mycoplasma protein antigen solution, and the content of the Cap protein is more than or equal to 160 [mu]g/ml; and the vaccine adjuvant is composed of a water-based high-molecular polymer adjuvant and a composite polysaccharide immunopotentiator. Foreign protein is removed through clarification filtration and ultrafiltration concentration, and the side reaction probability of the vaccine is greatly reduced; and three-proofing can be achieved through one needle, so that the number of immunization times and stress are reduced. The method is economical and practical, the immunization procedure is simplified, and the epidemic prevention cost is reduced.

The present invention relates to media formulations free of swine serum and animal (primarily bovine brain and spinal cord) origin ingredients for Mycoplasma growth. Media formulations are rationally designed to preserve Mycoplasma antigenicity. Mycoplasma grown in these media formulations are useful in vaccines, particularly multivalent swine vaccines.

The invention provides a method for preparing a serum-free mycoplasma bovis antigen. The method for preparing the serum-free mycoplasma bovis antigen comprises the following steps: (1) hatching freshSPF breeding eggs, carrying out culturing for 7-8 days, and selecting healthy SPF chicken embryos; (2) preparing a mycoplasma bovis inoculum; (3) inoculating the selected healthy SPF chicken embryos,which are 7-8 days old, with the mycoplasma bovis inoculum, continuing culturing after the inoculation in an incubator, observing status of the SPF chicken embryos, collecting dead inoculated SPF chicken embryos, and carrying out storing at 2-8 DEG C for 12-24 hours; and (4) aseptically collecting allantoic fluid of the dead SPF chicken embryos, thereby obtaining the serum-free mycoplasma bovis antigen. The method for preparing the serum-free mycoplasma bovis antigen is capable of realizing serum-free production of mycoplasma bovis antige, thereby avoiding multiple problems caused by use of animal serum; and moreover, the cultured mycoplasma bovis antigen is high in live bacterium titer.

The invention belongs to the technical field of biological detection, and particularly relates to a natural antigen P1 for detecting an anti-mycoplasma pneumoniae antibody as well as a preparation method and application of the natural antigen P1. The preparation method comprises the following steps of taking an inactivated human mycoplasma pneumoniae antigen culture solution, centrifuging to obtain mycoplasma pneumoniae thalli, washing with a mycoplasma washing solution, centrifuging, and discarding supernatant and precipitate to obtain mycoplasma pneumoniae; putting into a lysis solution, carrying out water bath incubation at 37 DEG C, carrying out cryopreservation at -20 DEG C, then carrying out re-melting, carrying out resuspension on the centrifuged precipitate by using a mycoplasma washing solution, and carrying out centrifugation to leave the precipitate; resuspending the precipitate in the lysis solution, carrying out ultrasonic treatment, and centrifuging to obtain a supernatant which is an antigen crude product; and carrying out centrifugal ultrafiltration on the supernatant obtained by filtering the crude product, and collecting the protein on the membrane, namely the natural antigen P1. The method is simple in step and suitable for popularization and mass production, and the prepared natural antigen P1 has good specificity and sensitivity to the mycoplasma pneumoniae antibody, so that the clinical detection sensitivity and specificity of the mycoplasma pneumoniae antibody in disease diagnosis are further improved.