40 results about "Antigen test" patented technology

The present invention relates to a method to prepare a reagent kit to quickly diagnose mycobacterium tuberculosis antigens jointly packaged by 38KD and 16KD antigens and a test method. The blood antigen test paper of the present invention jointly packages 38 KD and 16KD of TB antigens into a lower part of a NC film to form a test line (T). Goat anti-mouse IgG is packaged into an upper part of the NC film to form a quality control line (C). In addition, anti-human IgG (mouse anti-human IgG, goat anti-human IgG or SPA) marks nanometer coloring particles (colloidal gold particles or sol particles or nanometer beads) to prepare a labeling pad. The NC film, the labeling pad, a sampling pad and a sample adsorption pad are glued and assembled onto a plastic board to form the test paper. During test, few tested sample is added onto the NC film of the test paper. And then, sample diluents are added through a sampling end. After sampling, it is necessary to observe result of immunoreaction, thus realizing quick auxiliary TB antigen diagnosis.

The invention discloses a visual protein chip for detecting serum antibody of new-castle disease virus of chickens, infectious bronchitis virus of chickens, avian influenza virus and infectious bursal disease virus of chickens , which is prepared by the following steps: purifying and diluting whole proteins of the four virus respectively; pointing samples of the positive control serum, the negative control serum and the four virus proteins onto a chip carrier respectively; drying, fixing, sealing and washing the samples to obtain the visual protein chip. The visual protein chip uses the purified whole proteins as capturing antigens to detect the virus-specific antibodies in chicken serum so as to simplify the preparation technology and reduce the production cost, and the visual protein chip has better specificity but no cross, has high reliability of results and has the advantages of quickness, simplicity and convenience, high sensitivity, good specificity and the like. When the serum is diluted by 6,400 times, the visual protein chip still can detect the antibodies, the sensitivity is 400 times of that of the prior AGP detection method. According to the detection to serum samples in-place, the detection rate of the visual protein chip is higher than the proir AGP method remarkably.

The invention provides a three-in-one colloidal gold chromatographic test strip for detecting thiamphenicol, chloramphenicol and florfenicol and a preparation method thereof, and belongs to the technical field of immunological detection. The preparation method comprises two parts, namely, the preparation of a monoclonal antibody and the preparation of a colloidal gold chromatographic test strip, wherein the monoclonal antibody can be used for recognizing thiamphenicol, chloramphenicol and florfenicol at the same time and is high in sensitivity; the colloidal gold chromatographic test strip comprises a polyvinyl chloride backing; a sample pad is arranged at the front end of the polyvinyl chloride backing and is connected with the front end of a nitrocellulose membrane; the rear end of the nitrocellulose membrane is connected with a water absorbing pad; the monoclonal antibody marked by colloidal gold is used as a combining pad; the nitrocellulose membrane is sequentially wrapped with a chloramphenicol sodium succinate-BSA (Bovine Serum Albumin) antigen test ray T and a goat-anti-mouse IgG control ray C. The three-in-one colloidal gold chromatographic test strip for detecting thiamphenicol, chloramphenicol and florfenicol is fast to detect, and the detection needs only 3 to 5 minutes; the test strip is convenient to carry, and suitable for detection on site; the operation is simple and convenient and does not need a professional technical person.

The invention relates to a reagent kit for detecting antiuninuclear cell proliferation Listeria bacteria by colloidal gold Hly gene monoclonal antibodies and belongs to the technical field of hygiene inspection and medical inspection. The reagent kit is characterized by comprising the steps of: 1, primer design; 2, polymerase chain reaction (PCR) amplification; 3, agarose gel electrophoresis reaction conditions; 4, target deoxyribonucleic acid (DNA) segment cutting; 5, heat shock conversion method application; 6, reagent kit purification and plasmid preparation by a sodium dodecyl sulfate (SDS) cracking method; 7, protein induction expression; 8, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); 9, required strip cutting from the SDS-PAGE; and 10, colloidal gold preparation. The regent kit has the beneficial effect that the limitation aiming at the single-clostridium and single-antigen test in the prior art is avoided. The antibody is pure, the valence is high, and the sensitivity and the accurate degree are improved. The single added Listeria bacterium is detected. Compared with an enzyme-linked immuno sorbent assay (ELISA) method and a PCR method, the reagent kit has the advantage that the Listeria bacterium detection is faster and more convenient by the immune colloidal gold chromatography.

The invention discloses a cryptosporidium parvum recombinant antigen gene (i) MUCIN (/i) and an expressed fusion protein recombinant antigen rMUCIN. 520 parts of healthy human serum are randomly taken, the recombinant antigens rMUCIN and rCp23 are used as detection antigens, a human serum IgG antibody is detected by ELISA (enzyme-linked immunosorbent assay), spss software is used for analysis of data, and the results show that the rMUCIN protein and the rCp23 protein have the same positive rate in detection of unknown serum (XC2=0.370, and P=0.543>0.05), while the positive rate of the rMUCIN protein in detection of the unknown serum is higher than that of a cryptosporidium parvum crude antigen (XC2=5.222, and P=0.02<0.05). The invention further provides a kit for ELISA detection of the anti-cryptosporidium parvum IgG antibody, which takes the recombinant antigen rMUCIN as the detection antigen, takes the rCp23 as a positive control and has stronger specificity, sensitivity and reliability.

The invention discloses a human blood type test kit which comprises a test strip. The test strip comprises a gold conjugate pad and a reaction membrane, and the gold conjugate pad is coated with a colloidal gold-labelled mouse anti-human A antigen monoclonal antibody, a colloidal gold-labelled mouse anti-human B antigen monoclonal antibody and a colloidal gold-labelled mouse anti-human D antigen monoclonal antibody; the reaction membrane comprises a B antigen test line coated with the mouse anti-human B antigen monoclonal antibody, an A antigen test line coated with the mouse anti-human A antigen monoclonal antibody, an Rh test line coated with the mouse anti-human D antigen monoclonal antibody and a quality control line coated with a goat anti-mouse IgG polyclonal antibody. The invention further provides a preparation method of the kit. The preparation method comprises the steps of reaction membrane preparation, gold conjugate pad preparation, cutting assembly and the like. The human blood type test kit can simultaneously test A, B, O and Rh blood types, is intuitive in test result, can be used for testing hemolysis samples and has the high sensitivity, specificity and stability.

The invention provides a porcine circovirus type 4 ELISA antibody detection kit, application and a method for detecting a porcine circovirus type 4 antibody, and relates to the technical field of biology. The porcine circovirus type 4 ELISA antigen detection kit provided by the invention comprises an elisa plate coated with porcine circovirus type 4 Cap protein. The ELISA antibody detection kit isestablished by using the circovirus type 4 Cap protein as an antigen, and the Cap protein as a circovirus conservative protein has good antigenicity and can be specifically combined with a circovirustype 4 antibody in a serum sample, so that the kit provided by the invention has very strong specificity and sensitivity to the circovirus type 4.

The invention relates to the technical field of immunodiagnosis, and discloses colloidal gold immunochromatography detection test paper for rapidly diagnosing brucellosis and a preparation method of the colloidal gold immunochromatography detection test paper for rapidly diagnosing brucellosis in order to solve the problem of inaccurate detection results caused by antigen detection of brucellosisantibodies in the prior art. The method specifically comprises the following steps: 1) preparing a detection line T and a quality control line C; 2) preparing a gold-labeled pad; 3) preparing the testpaper: sequentially connecting and assembling the water absorption pad, the sample pad, the microsphere probe treatment pad, the antibody-coated modified nitrocellulose membrane and the PVC bottom plate to obtain a finished product. Whether brucellosis exists or not can be accurately detected, the product adopts an antibody antigen detection mode, and the antigen has no incubation period; compared with a product for detecting an antigen, the test paper is more accurate, the antigen detection accuracy is higher than that of a product for detecting the antibody, the detection efficiency is high, and the preparation process is simple.

The invention discloses a colloidal gold chromatography reagent strip for detecting a new crown antigen, a preparation method thereof and a kit. The colloidal gold chromatography reagent strip comprises a bottom plate, a nitrocellulose membrane, a coupling pad, a sample pad and absorbent paper, the sample pad, the coupling pad, the nitrocellulose membrane and the absorbent paper are sequentially connected to the bottom plate in the horizontal direction, and avidin biotin amplified colloidal gold nanoflowers are coupled to the coupling pad. The nitrocellulose membrane is modified by nanocellulose, a detection line coated with a new crown antigen capture antibody and a quality control line coated with a quality control molecule capture antibody are arranged on the nitrocellulose membrane, and the detection line and the quality control line are sequentially distributed in the chromatography direction. The colloidal gold chromatography reagent strip can be used for conveniently and quickly detecting new crown antigens of nasopharynx swabs and throat swabs with high sensitivity.

The invention belongs to the technical field of antigen detection, and particularly relates to an integrated antigen detection device and an antigen detection process quality control method thereof, and the method comprises the following steps: obtaining a first image of a nasal swab part inserted into a nasal cavity; first judgment is carried out based on the first image, and whether sample sampling of the detected person is qualified or not is judged. The problem that an infected person cannot be found in time due to the fact that the self-testing process of the detected person is not standard, misoperation or intentional cheating exists in the prior art is solved; the method has the advantages that the self-test process of the subject is standardized, misoperation or intentional cheating behaviors of the subject in use are effectively prevented, and therefore the infected person is effectively found in time.

The invention discloses colloidal gold test paper for detecting an A-beta antigen in senile dementia serum and a use method of the colloidal gold test paper, and relates to the technical field of whole blood sample pretreatment, the colloidal gold test paper comprises a labeling pad, and is characterized in that two detection lines are arranged on the labeling pad respectively and used for detecting the A-beta antigen, one detection line is coated with an antibody capable of recognizing A-beta40, and the other detection line is coated with an antibody capable of recognizing A-beta42. According to the colloidal gold test paper, a whole blood sample is pretreated by using a brand new designed Tris buffer solution, so that A-beta40 and 42 of the blood sample to be detected can be detected on the colloidal gold test paper, and the colloidal gold test paper is suitable for senile dementia patients who need to monitor the course of Alzheimer's disease at any time or are in the period of medicine replacement.

The invention discloses an antigen test kit suitable for self-service detection and a data acquisition system. A chromatography detection card of the antigen test kit is arranged in a test kit card shell; a serial number window, a result observation window and a sample adding opening are sequentially formed in the top surface of the test kit clamping shell upper cover; the sample pad and the water absorption pad are arranged at the two ends of the reaction pad, and the sample pad, the water absorption pad and the reaction pad are fixedly arranged between the test kit clamping shell upper cover and the test kit clamping shell bottom cover. And a background database of the antigen test data acquisition system stores a serial number of an antigen test kit, text information of a detection bar code and a corresponding detection result. The detection result is displayed in an encrypted pattern mode, the pattern or the decrypted text cannot be used for judging the detection result, the detection result can be obtained only by matching the pattern or the decrypted text with the serial number in a data acquisition system, detection is accurate and rapid, the device is simple and easy to popularize, and accurate and rapid centralized acquisition of the detection result can be achieved.