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Device for collecting supernate in dog manure antigen ELISA (Enzyme Linked Immunosorbent Assay) detection

A supernatant and antigen technology, applied in the direction of sampling devices, etc., can solve the problems of scum, slow liquid absorption process, more detection time and manpower, etc., achieve low manufacturing cost, easy to use and operate, and overcome liquid absorption obstacles Effect

Pending Publication Date: 2017-01-25
STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For dog feces samples, due to the large amount of impurities, the pretreatment is more complicated, and steps such as crushing, grinding and mixing with diluent, centrifugation and supernatant are required, which take up a relatively large amount of testing time and manpower.
Dog feces samples often have a scum when aspirating the supernatant, resulting in a slower aspiration process

Method used

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  • Device for collecting supernate in dog manure antigen ELISA (Enzyme Linked Immunosorbent Assay) detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Echinococcus canis antigen ELISA detection sample pretreatment without centrifugation directly supernatant collection

[0025] step:

[0026] 1. Pretreatment of dog feces samples: Pre-frozen dog feces samples at low temperature (-70°C) for 14 days.

[0027] 2. Dilute the concentrated sample diluent with deionized water at a ratio of 1:1, and mix well to obtain the sample diluent.

[0028] 3. Weigh 1g of dog feces sample and add it to a deformable plastic hose with a diameter of 1cm and a length of 10cm (ie, the outer casing 2), then add 1ml of sample diluent, stir well, and let stand for 10min.

[0029] 4. A hard straight filter tube 1 with a pipe diameter of 0.9 cm and a tube length of 8 cm prepared by plastic (the bottom of the filter tube 1 has small holes 3 with a diameter of 100 μm on the wall, and the number of small holes 3 is 30. ) into the outer casing 2, see figure 1 .

[0030] 5. Aspirate 100 μL of supernatant according to the instructions of th...

Embodiment 2

[0031] Example 2 Echinococcus canis antigen ELISA detection sample pretreatment after centrifugation and direct supernatant collection

[0032] 1. Pretreatment of dog feces samples: Pre-frozen dog feces samples at low temperature (-70°C) for 14 days.

[0033] 2. Dilute the concentrated sample diluent with deionized water at a ratio of 1:1, and mix well to obtain the sample diluent.

[0034] 3. Weigh 1g of dog feces sample and add it to a deformable plastic hose with a diameter of 1.2cm and a length of 10cm (i.e. the outer casing 2), then add 1ml of sample diluent, stir well, and centrifuge at 3000rpm / min for 10min .

[0035] 4. A hard straight filter tube 1 with a diameter of 1.1 cm and a tube length of 6 cm prepared by plastic (the bottom tube wall of the filter tube 1 has small holes 3 with a pore size of 100 μm, and the number of small holes 3 is 30. ) into the outer casing 2, see figure 1 .

[0036] 5. Aspirate 100 μL of supernatant according to the instructions of the...

Embodiment 3

[0037] Example 3 Echinococcus canis antigen ELISA detection sample pretreatment after centrifugation and collection of supernatant

[0038] 1. Pretreatment of dog feces samples: Pre-frozen dog feces samples at low temperature (-70°C) for 14 days.

[0039] 2. Dilute the concentrated sample diluent with deionized water at a ratio of 1:1, and mix well to obtain the sample diluent.

[0040] 3. Weigh 1g of dog feces sample and add it to a deformable plastic hose (outer casing 2) with a diameter of 1.5cm and a length of 10cm, then add 1ml of sample diluent, stir well, and centrifuge at 3000rpm / min for 10min.

[0041] 4. A hard straight filter tube 1 with a diameter of 1.4 cm and a tube length of 7 cm prepared by plastic (the bottom tube wall of the filter tube 1 has small holes 3 with a diameter of 100 μm, and the number of small holes 3 is 30. ) into the outer casing 2, see figure 1 .

[0042] 5. Aspirate 100 μL of supernatant according to the instructions of the ELISA manufactu...

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Abstract

The invention discloses a device for collecting supernate in dog manure antigen ELISA (Enzyme Linked Immunosorbent Assay) detection. The device comprises a filtering tube and an outer-layer casing, wherein holes are formed in the filtering tube; the filtering tube is arranged inside the outer-layer casing. When being used, the outer-layer casing is filled with a dog manure sample and a diluent; after being sufficiently and uniformly mixed, the dog manure sample and the diluent are not centrifuged or centrifuged for 10 minutes at a speed of 3000rpm / min, subsequently, the filtering tube is added into the outer-layer casing, and then the supernate can be directly detected or is sucked out for detection. The device can be adopted to collect the supernate after centrifugation in the pretreatment process of the dog manure sample, the supernate is not affected by floating impurities in the sample, the supernate can be collected without obstacle, the testing process can be accelerated, and the labor and the time can be saved.

Description

technical field [0001] The invention relates to a device for experimental detection, in particular to a device for collecting supernatant in the detection of canine fecal antigen enzyme-linked immunosorbent assay (ELISA). Background technique [0002] The ELISA experiment is based on the immunological combination of antigen and antibody as the basic principle, and has been widely used in the determination of antigen or antibody substances, especially successfully applied to infectious diseases caused by various viruses, bacteria and parasites and other biomolecular recognition. Immunological diagnosis of animal diseases and other aspects. In addition to the good sensitivity and specificity of the ELISA method, the operation process is simple and fast, and it is easy to automate. At present, there is a fully automated ELISA detection system. Therefore, a small laboratory can check hundreds or even thousands of specimens within one day by using the ELISA method, which is suit...

Claims

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Application Information

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IPC IPC(8): G01N1/14
CPCG01N1/14
Inventor 肖宁刘辉徐克均曹淳力杨诗杰田添王东彭佳字金荣李奔富
Owner STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
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