Vaccine composition for preventing and treating porcine respiratory syndrome, and preparation method and application thereof
A vaccine composition and respiratory technology, applied in the field of veterinary vaccines, can solve problems such as the ineffectiveness of antibacterial drugs, achieve the effects of reducing production costs and epidemic prevention costs, simplifying immunization procedures, and avoiding adverse reactions
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0043] Preparation of three antigens in the vaccine composition of embodiment 1, swine influenza virus, mycoplasma hyopneumoniae, Haemophilus parasuis
[0044] 1. Preparation of swine influenza virus (ZJS strain and WX strain) antigen
[0045] Spinner bottle cell culture method. Inoculate SIV H1N1 subtype ZJS strain and H3N2 subtype WX strain virus seeds into monolayer MDCK cells (purchased from ATCC, No. CRL-34) according to the inoculum amount of M.O.I=0.1, absorb at 37°C for 30 minutes, add DMEM cell maintenance solution containing 3% (v / v) calf serum, cultured at 37°C with rotation (10-12 revolutions / hour). Observe 1-2 times a day, the cells grow well, culture at 37°C for 2-5 days, harvest the cells and cell liquid, freeze and thaw 3 times, separate the two virus liquids (ZJS strain and WX strain) with hollow fibers (pore size 1.2 μm) Filter with a 0.45μm) filter column to remove cell debris, then add 0.2% to 0.3% formaldehyde solution to inactivate at 37°C for 18h, and ...
Embodiment 2
[0071] Embodiment 2, preparation of swine influenza virus antigen, Haemophilus parasuis antigen, Mycoplasma swine pneumonia antigen vaccine composition:
[0072] In order to compare the immune effects of vaccine compositions with different antigen content, two kinds of adjuvants were selected to prepare the vaccine composition, namely Montanide TM Gel (produced by SEPPIC, France), aluminum glue, white oil, squalene, and each adjuvant were used to prepare vaccine compositions for swine influenza virus antigen, Haemophilus parasuis antigen, and mycoplasma swine pneumonia antigen respectively. The specific configuration is as follows:
[0073] 1. Use Gel adjuvant vaccine
[0074] Preparation method: the formula is as shown in Table 2. Take the swine influenza antigen, Haemophilus parasuis antigen and Mycoplasma hyopneumoniae antigen prepared in Example 1. The concentrated five kinds of antigen solutions were prepared into mixed antigen solutions or directly prepared into antig...
Embodiment 3
[0100] Embodiment 3, the immune effect evaluation of vaccine composition to pig
[0101] 1 Test piglets immunized with vaccine
[0102] Vaccine composition (vaccine 1, vaccine 2, vaccine 3, vaccine 4, vaccine 5, vaccine composition (vaccine 1, vaccine 2, vaccine 3, vaccine 4, vaccine 5, Vaccine 6, Vaccine 7, and Vaccine 8) were used for testing after passing the sterility inspection.
[0103] A total of 45 groups of 225 piglets aged 14-21 days were selected, 5 pigs / group. There were 240 immune groups. The triple vaccine composition of the present invention with each adjuvant was used to immunize 10 groups of 50 piglets. There were 5 groups of 25 piglets in the control group. The specific groups of immunized piglets and the number of immunized piglets are shown in Table 6; according to the groups, each pig was injected with 2ml of the corresponding inactivated vaccine in the neck muscle, and 2ml was inoculated again 14 days after immunization; at the same time, 5 groups of ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com