Method for preparing serum-free mycoplasma bovis antigen
A Mycoplasma bovis, serum-free technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacterial antigen components, etc., can solve the problems of pollution, bovine body affecting the immune effect, serum increasing the cost of vaccine production, etc., to achieve stable sources , easy to operate, easy to obtain the effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] A method for preparing Mycoplasma bovis antigen without serum is as follows:
[0029] (1) Put fresh SPF eggs in an incubator to incubate (temperature 37.0-37.5°C, humidity 50%), cultivate for 7 days, illuminate the eggs before inoculation, pick healthy chicken embryos with well-developed, clear blood vessels, and vigorous vitality, discard Remove non-fertilized eggs, dead embryos and weak embryos, draw the air cell position of healthy chicken embryos in the dark room, and reserve the chicken embryos.
[0030] (2) Preparation of Mycoplasma bovis inoculum: resuscitate and subculture Mycoplasma bovis PG45 strain (ATCC, No. ATCC 25523), culture in a constant temperature incubator at 37°C for 24 hours, the medium is modified Thiaucourt's medium, and the color of the medium is determined. Turn yellow (or when the pH value drops from 7.4 to 7.0), centrifuge the fresh Mycoplasma bovis culture bacterium liquid, centrifuge 15min at 8000rpm, resuspend the thalline with equal volum...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com