Kit and detection method for detecting mycoplasma pneumoniae nucleic acid and drug resistance gene variation of mycoplasma pneumoniae nucleic acid

Abstract

The invention provides a kit and a detection method for detecting mycoplasma pneumoniae nucleic acid and drug-resistant gene variation, which are beneficial for detecting the mycoplasma pneumoniae nucleic acid and drug-resistant gene variation of the mycoplasma pneumoniae nucleic acid and the drug-resistant gene variation of the mycoplasma pneumoniae nucleic acid. The kit and the detection method for detecting mycoplasma pneumoniae nucleic acid and drug-resistant gene variation of the mycoplasma pneumoniae nucleic acid are characterized in that the kit comprises KOD DNA polymerase, a primer MPN-F, a primer MPN-R and a specific targeting fluorescent probe. According to the kit and the detection method disclosed by the invention, the aim of rapidly, simply, conveniently and accurately detecting the mutations of the mycoplasma pneumoniae nucleic acid, the 23S rRNA gene of the mycoplasma pneumoniae nucleic acid and the 2063th or 2064th gene locus of the 23SrRNA is fulfilled by designing the primers and the probes with specific sequences.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular, to a detection kit and a detection method for Mycoplasma pneumoniae nucleic acid and its drug-resistant gene variation. The kit is used to qualitatively detect sputum samples, throat swab samples, or The Mycoplasma pneumoniae 23S rRNA gene and its variation in nasal swab samples are suitable for auxiliary diagnosis of Mycoplasma pneumoniae infection. Background technique [0002] Mycoplasma pneumoniae (M Pneumonia, also referred to as MP or Mp) is the pathogen of human mycoplasma pneumonia, which is mainly transmitted by droplets, with an incubation period of 2 to 3 weeks. At present, Mycoplasma pneumoniae has become one of the main pathogens of community-acquired pneumonia in children, and it is also a common pathogen of respiratory tract infections in adolescents and elderly people. MP is sensitive to macrolide antibiotics that inhibit microbial protein synthesis, quin...

AI Technical Summary

Problems solved by technology

Considering that the concentration of Mp in the patient's sputum sample is about 10 2 ~10 6 cfu / ml, while the detectable concentration range of antigen detection technology is 10-100cfu / ml, the sensitivity of antigen detection without Mp amplification and culture is very limited, so when nucleic acid amplification technology can be used for diagnosis, antigen detection is not recommended to diagnose

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