36results about How to "Short color development time" patented technology

The invention relates to the mensuration method for amorphous Fe in soil, in particular to leach the soil with hydroxylamine for amorphous Fe and then mensurate it adopting the Visible spectrophotometry method. The specific steps are as follows: adding hydrochloric acid solution and hydroxylamine hydrochloride solution to the soil sample, churning for 30 - 60 seconds and placing at room temperature for 1-2 hours before filtration; Taking 5-20 ml filtered extract solution and adding 1.00-2.00 ml hydroxylamine hydrochloride solution and shaking up, and laying aside for 5-10 minutes to make the Fe(III) completely deacidized to Fe(II), and then adding sodium acetate solution to modify the PH value of the solution to 3-6, and adding a visualization reagent phenanthroline and shaking up the solution and laying aside at room temperature for 30-40 minutes for developing color, at last mensurating the contents of amorphous Fe in the soil sample on spectrophotometer with a wavelength of 520mn. The method of the invention is simple and easy to be promoted. The analysis method is fast and precise in realizing the mensuration of the amorphous Fe in soil.

The invention discloses a method for measuring and analyzing Fe content of a carbon material. The method is characterized by comprising the following steps of: melting the carbon material by using anhydrous sodium carbonate or / and boracic acid as a melting agent, extracting the sample by using a diluted hydrochloric acid solution, reducing Fe (III) in the sample into Fe (II) by using hydroxylamine hydrochloride, regulating the pH value, adding a color development agent, and analyzing and determining the Fe content by using a colorimetric method. The method for measuring and analyzing the Fe content of the carbon material is easy to operate and high in accuracy.

The invention discloses ammonia nitrogen test paper and an application method thereof. The test paper is prepared according to the following steps: (1) preparing a reagent solution, namely dissolving analytically pure sodium salicylate, sodium potassium tartrate tetrahydrate and sodium nitroprusside in ammonia-free water to prepare a solution in which the concentration of the sodium salicylate is 280g / L to 380g / L, the concentration of the sodium potassium tartrate tetrahydrate is 50g / L to 68g / L and the concentration of the sodium nitroprusside is 0.40g / L to 0.54g / L; (2) soaking, namely completely soaking quantitative immediate-speed filter paper in the solution (1), oscillating for 60 to 120 minutes on a constant-temperature oscillator; (3) molding, namely taking out the filter paper, arranging in parallel at room temperature, suspending, drying in air, cutting and sealing by plastic for later use. The test paper is simple to prepare, convenient to operate, short in color development time, high in removability and capable of being used for rapidly and accurately testing the content range of ammonia nitrogen in water.

The invention discloses a hollow copper sulphide / polypyrrole nanometer compound which has activity similar to peroxidase, can generate hydroxyl radicals with strong oxidizability by catalyzing H2O2, and is applicable to detection and analysis of glucose content in food and blood, or oxidation degradation of organic pollutants in sewage. The nanometer compound of the present invention combines the advantages of copper sulphide and polypyrrole; after compounding, the concerted catalysis effect of the hollow copper sulphide / polypyrrole nanometer compound is obviously better than only copper sulphide particles or polypyrrole particles; the compound has the significant advantages of high catalytic efficiency, short developing time, few using amount, stable chemical activity, wide application scope, and the like, can be used as a substitute for natural peroxidase, and is applicable to detection of glucose content in food and biology, and oxidation degradation of organic pollutants in environmental sewage.

The invention relates to an electric enhanced color development method and a special device thereof for rapid detection of lead ions or ferrous ions, a metal sheet is used as a support carrier, a polyester fiber film is used as a color development agent adsorption carrier, the polyester fiber film is coated with a color development agent dissolved in an ionic liquid to prepare a color development plate, the color development plate is used as an electric enhanced color development working electrode, a platinum wire electrode is used as a counter electrode for combination with a direct-current power supply. According to the electric enhanced color development method and the special device thereof, the characteristics that the ionic liquid is insoluble in water and can dissolve the color development agent are used, a traditional spectrophotometry method for detection of the lead ions or the ferrous ions is transplanted to the color development plate, the method is simple, the operation is easy, the flexibility is high, and the electric enhanced color development means is introduced, so that the color development time is shortened, the detection sensitivity is improved, the detection limit reaches stipulated limits of various water quality standards, and the electric enhanced color development method and the special device thereof can be widely applied to qualitative and semi quantitative detection of the lead ions and ferrous ions in various water samples and have strong practical application values.

The present invention provides a method of supplementing animals with carotenoids by drinking water, comprising the following steps of: (1) making yellow and / or red pigments into the microencapsulated dry powders or beadlets; (2) mixing the aforementioned microencapsulated dry powders or beadlets in a certain proportion and dissolving them in water to prepare for a pigment solution; (3) administering the prepared pigment solution to animals by drinking. The method of supplementing the carotenoids according to the present invention has the advantages of easy absorption, economical dosages of pigments, high efficiency of coloration, stable coloration and small effects on the process of pigments in comparison with conventional methods currently.

The invention provides a micro-culture method of pleuromutilin producing bacteria. The method comprises the following steps of: culturing the pleuromutilin producing bacteria and then separating a single bacterial colony; preparing a solid fermentation culture medium and sterilizing at a high pressure, and then adding the sterilized solid fermentation culture medium into each pore of an enzyme label plate; inoculating mycelia of the single bacterial colony generated by the pleuromutilin producing bacteria into the enzyme label plate and culturing for 7-9 days. The invention also provides a high-throughput screening method of high-yield bacteria of the pleuromutilin. The high-throughput screening method comprises the following steps of: adding extraction liquid into the pores of the enzymelabel plate of the micro-cultured pleuromutilin producing bacteria to extract; sucking the extraction liquid for each pore and transferring to another enzyme label plate; adding a color-developing agent to develop; measuring by using an enzyme label meter and analyzing the result to obtain the screened high-yield bacteria of the pleuromutilin. As the solid fermentation is adopted, the step of culturing seeds is simplified and the fermentation time is greatly shortened. Compared with the common screening method, the high-throughput screening method has a good related coefficient, thereby beingapplied to screening of the high-yield bacteria of the pleuromutilin.

The invention discloses a feline herpes virus type I gB-gD recombinant protein, and belongs to the field of animal virus antibody detection. The recombinant protein comprises an amino acid sequence asshown in SEQ ID NO.1 or consists of an amino acid sequence as shown in SEQ ID NO.1. The invention further discloses a gene of the recombinant protein. The gene comprises a vector containing the gene,a host cell. The invention also discloses a preparation method of the recombinant protein and application of the recombinant protein in detection of feline herpes virus type I antibody. The recombinant protein is used for detecting the feline herpes virus type I antibody, is convenient and rapid, has high sensitivity, does not have cross reaction with other pathogens, has high specificity, and has huge clinical significance and wide application prospect.

The invention provides a method for determining and analyzing Si content in a carbon material. The method is characterized by comprising the following steps of: with anhydrous sodium carbonate or / and boric acid as a solvent, melting the ash obtained by firing the carbon material; leaching out a test solution by use of a diluted hydrochloric acid solution; adjusting the PH value of the test solution, and then adding ammonium molybdate so that the silicon forms silicon-molybdenum heteropoly acid; reducing the silicon-molybdenum heteropoly acid into silicon-molybdenum blue by a reducing agent in a high-acidity sulfuric acid medium; and analyzing and determining the Si content by a colorimetric method. The method for determining and analyzing Si content in a carbon material, provided by the invention, is simple to operate and has high accuracy.

The invention provides a gene expression component, a constructed cloning vector and application. The gene expression component encodes beta-galactosidase alpha peptide and omega peptide; the gene expression component includes a constitutive promoter, and the constitutive promoter promotes an expressed LacZ[alpha] gene and LacZ[omega] gene. The constitutive promoter is used to regulate the expression of the LacZ[alpha] gene and LacZ[omega] gene, a constructed pWizard plasmid can produce substances with beta-galactosidase activity in a host cell without IPTG induction, the application scope ofthe host cell is expanded, the pWizard plasmid is a high-copy plasmid, therefore, the copy number of the LacZ[alpha] gene and the LacZ[omega] gene in a host bacteria is high, the expression amount ofthe host cell to an enzyme with beta-galactosidase activity is significantly increased, the color development effect and the color development speed are enhanced, and the color development time is shortened.

A total cholesterol detection method comprises steps as follows: 1) a detection reagent and a test strip thereof are prepared; 2) total cholesterol whole blood control products with different concentrations are prepared; 3) the total cholesterol whole blood control products with different concentrations in the step 2) are dropwise added to the test strip obtained in the step 1), the test strip displays different colors, total cholesterol concentration detection is performed on the total cholesterol whole blood control products with different concentrations by use of a full-automatic biochemical analyzer, the test strip color developing results are calibrated according to detection results of the total cholesterol whole blood control products with the same concentration by use of the full-automatic biochemical analyzer, and a colourimetric card is obtained; 4) when the test strip obtained in the step 1) is used for detecting the total cholesterol concentration of a sample, the sample isdropwise added to the test strip, the color developing result of the test strip is compared with the colourimetric card in the step 3) after 2-3 min, and the concentration of the total cholesterol inthe sample is obtained. The cost and the time can be saved, and the stability, repeatability and sensitivity of the method can be guaranteed.

The invention discloses a preparation method of peroxidase-like nano-enzyme beta-FeOOH and application of peroxidase-like nano-enzyme beta-FeOOH in H2O2 detection, and belongs to the technical field of inorganic nano-materials. The peroxidase nano-enzyme beta-FeOOH is obtained through a simple one-step low-temperature hydrolysis precipitation method, H2O2 can be catalyzed to generate hydroxyl freeradicals with high oxidability, and TMB is catalyzed to generate a chromogenic reaction in an extremely short time. Compared with a natural enzyme, the peroxidase-like nano-enzyme beta-FeOOH preparedby the method has the advantages of high stability, easiness in storage and simple process, in addition, the peroxidase-like nano-enzyme beta-FeOOH prepared by the method has higher affinity with H2O2, and is expected to be applied to detection of low-concentration H2O2.

The invention relates to an assaying method for Ni content in a carbon material. The assaying method is characterized by comprising the steps of: burning the carbon material with anhydrous sodium carbonate or / and boric acid as a flux, carrying out fusion on ash obtained after burning, leaching with a dilute hydrochloric acid solution to obtain a test solution, reacting nickel and dimethylglyoxime in an alkaline medium in the existence of an oxidant to generate a soluble claret complex, and measuring Ni content by colorimetry. The assaying method for Ni content in the carbon material, provided by the invention, is simple in operation method and high in accuracy.

The invention discloses a B.canis BcMSA1-BcSA1 recombinant protein, and belongs to the field of animal virus antibody detection. The recombinant protein comprises an amino acid sequence shown in SEQ ID NO. 1 or is composed of the amino acid sequence shown in SEQ ID NO. 1. The invention further discloses a gene of the recombinant protein, a carrier and a host cell containing the gene, a preparationmethod of the recombinant protein and application of the recombinant protein to detection of an antibody of B.canis. The recombinant protein is used for detecting the antibody of B.canis, is convenient and fast to use, high in sensitivity, free of cross reaction with other pathogens and high in specificity, and has great clinical significance and wide application prospects.

The invention discloses test paper for rapidly detecting concentration of an o-phthalaldehyde disinfectant and a preparation method and application of the test paper. The test paper comprises a substrate and filter paper, wherein the filter paper is stuck to the substrate and is dipped with a fixative solution, a solution A and a solution B, wherein the solution A comprises the following ingredients: 0.2% to 4% of adsorbent, 0.2% to 16% of colored indicator, 0.1% to 15% of water-soluble solid organic acid and 0.02% to 5% of suspension dispersant; the solution B comprises the following ingredients: 0.2% to 6% of adsorbent, 0.1% to 6% of suspension dispersant and 0.2% to 10% of adhesive aqueous solution. The test paper for rapidly detecting the concentration of the o-phthalaldehyde disinfectant, provided by the invention, can be used for rapidly and accurately detecting the content of o-phthalaldehyde. The test paper facilitates that medical staff can monitor the concentration of o-phthalaldehyde at any time, and the operation is simple and rapid, so that the safety of disinfection effect of medical appliances is guaranteed.

The invention relates to an in vitro diagnostic reagent and a preparation method thereof and in particular relates to a rapid detection kit for drug susceptibility of mycoplasma bovis and a preparation method of the rapid detection kit. The invention provides the rapid detection kit for the drug susceptibility of the mycoplasma bovis; the kit comprises a mycoplasma culture solution, a drug susceptibility plate and an antibiotic drug. The mycoplasma culture solution is prepared from the following components according to the content: 10 to 15g of PPLO (Pleuropneumonia-like Organism) powder, 2 to4g of oligopeptide, 100 to 500mg of morroniside, 2 to 6g of yeast powder, 1 to 3g of glucose, 1.5 to 4.5g of sodium pyruvate, 80 to 120mL of an MEM culture medium, 2mL of a 1 percent (w / v) phenol redsolution, 100 to 300mL of horse serum, 0.3 million units of penicillin and 700 to 800mL of ddH2O. The culture solution provided by the invention can be used for rapidly promoting the growth of the mycoplasma bovis and detection can be finished within 20 to 24h; the rapid detection kit has stable performance and strong specificity.

A test paper for detecting ammonia nitrogen and its application method. The test paper is prepared by the following method, including the following steps: (1) Reagent solution preparation: take analytically pure sodium salicylate, potassium sodium tartrate and sodium nitroferricyanide solution In ammonia-free water, prepare a solution containing 280-380 g of sodium salicylate, 50-68 g of sodium potassium tartrate and 0.40-0.54 g of sodium nitroferricyanide per liter; Immerse all the fast filter paper in the solution (1), and shake it on a constant temperature oscillator for 60-120 minutes; (3) Forming: Take out the filter paper, place it in parallel at room temperature, hang it in the air to dry, cut it and seal it for later use. The test paper is simple to make, easy to operate, short in color development time and strong in mobility, and can quickly and accurately determine the content range of ammonia nitrogen in the effluent.

The invention belongs to the technical field of medical examination, and particularly relates to a rapid detection kit for bacterial vaginosis, a preparation method and application thereof. The kit comprises a reagent R1 and a reagent R2, and the reagent R1 is composed of 5-bromine-4-chlorine-3-Indolyl alpha-D-N-acetylneuramidate, polyethylene glycol 200 and phosphate buffer solution; and the reagent R2 is composed of 4-amino antipyrine and the phosphate buffer solution. The rapid detection kit for the bacterial vaginosis has the advantages of high determination sensitivity, high accuracy of detection results, good repeatability and high stability, and is an ideal rapid detection kit for the bacterial vaginosis, and the popularization and application of the kit are facilitated.

The invention relates to an in vitro diagnostic reagent and a preparation method thereof and in particular relates to a rapid detection kit for drug susceptibility of mycoplasma bovis and a preparation method of the rapid detection kit. The invention provides the rapid detection kit for the drug susceptibility of the mycoplasma bovis; the kit comprises a mycoplasma culture solution, a drug susceptibility plate and an antibiotic drug. The mycoplasma culture solution is prepared from the following components according to the content: 10 to 15g of PPLO (Pleuropneumonia-like Organism) powder, 2 to4g of oligopeptide, 100 to 500mg of morroniside, 2 to 6g of yeast powder, 1 to 3g of glucose, 1.5 to 4.5g of sodium pyruvate, 80 to 120mL of an MEM culture medium, 2mL of a 1 percent (w / v) phenol redsolution, 100 to 300mL of horse serum, 0.3 million units of penicillin and 700 to 800mL of ddH2O. The culture solution provided by the invention can be used for rapidly promoting the growth of the mycoplasma bovis and detection can be finished within 20 to 24h; the rapid detection kit has stable performance and strong specificity.

The invention discloses an RHDV VP10-VP60 recombinant protein, and belongs to the field of animal virus antibody detection. The recombinant protein comprises an amino acid sequence as shown in SEQ IDNO.1 or consists of an amino acid sequence as shown in SEQ ID NO.1. The invention also discloses a preparation method of the recombinant protein. The invention further discloses a gene of the recombinant protein, a vector containing the gene, a host cell containing the gene, a preparation method of the recombinant protein and application of the recombinant protein in an antibody for detecting RHDV. The recombinant protein disclosed by the invention is used for detecting the RHDV antibody, is convenient and rapid, has high sensitivity, does not have cross reaction with other pathogens, has strong specificity, and has huge clinical significance and wide application prospect.

The invention provides a recombinant antigen protein rP44-60 for detecting granulocytoplasmosis and a kit containing the antigen, and belongs to the technical field of immunology, and the recombinant antigen protein comprises an amino acid sequence as shown in SEQ ID NO.1 in a sequence table. The kit comprises a recombinant antigen protein rP44-60, a sample pad, a combination pad, an absorption pad, a nitrocellulose membrane, a colloidal gold probe, mouse IgG and goat anti-mouse IgG. The recombinant antigen protein disclosed by the invention is high in sensitivity and strong in specificity, and can be used for rapidly detecting an antibody in anaplasma phagocytophilum infected serum, so that the problem that the antibody is difficult to capture due to antigen variation of HGA is solved, the defect of existing antigen missing detection is made up, and the missed diagnosis rate is effectively reduced; according to the preparation method, cross reactivity caused by irrelevant sequences is reduced, and the expression quantity and stability of recombinant antigen protein are high; the kit has the characteristics of rapidness, sensitivity, high specificity, no limitation of experimental conditions and the like, and has low requirements on detection conditions.

The invention discloses a rapid detection method and a detection kit for melatonin. The rapid detection method for the melatonin comprises the following subsequent steps: firstly, mixing a to-be-tested sample with an extractant, standing and taking supernatant as probational liquid; secondly, dropping the probational liquid on qualitative filter paper; after drying in air, and dropwise adding a p-dimethylaminobenzaldehyde solution at the titration position of the probational liquid; observing the color change of the filter paper; if the color of the filter paper turns blue, showing that the to-be-tested sample contains the melatonin; if the color of the filter paper has no change, showing that the to-be-tested sample does not contain the melatonin. The rapid detection method disclosed by the invention has the advantages of simple operation, short time and high efficiency; compared with methods of high performance liquid chromatography, chromatography-mass spectrometry, enzyme-linked immunity and the like, the rapid detection method has the characteristics of simplicity, fastness and no need of reference substances; high accuracy is realized, and common ingredients such as tablets,capsules, pills, granules and oral liquid have no interference on the detection of melatonin substances; the sensitivity is high and the detection limit is smaller than 0.04mg; consumables are few aswell as environmental protection and safety are achieved.

The invention belongs to the field of nanometer technologies and particularly relates to a method for efficiently catalyzing a TMB (Tetramethylbenzidine) color development reaction by using carbon nanoparticles. The method comprises the steps of: adding 250 mu M of TMB solution and 0.25-500 mu M of H2O2 into 400 mu L of water, then adding 5-40 mu L of surfactant-modified carbon nanoparticles, shaking to reach a uniform state, and measuring time-varying absorption values under the wavelength of 652nm, wherein the surfactant is anyone of SDS (Sodium Dodecyl Sulfate), DNA (Deoxyribose Nucleic Acid), DTAB (Dodecyl Trimethyl Ammonium Bromide) and Tuwen20, and the dimension of the carbon nanoparticles is 20-70nm. By utilization of the carbon nanoparticles as a catalyst, the favorable characteristics of cheap materials, short color development time, high catalysis efficiency and the like are achieved, and the catalytic effect of the carbon nanoparticles is far better than that of graphene and single-walled carbon nanotubes.

The invention relates to an assaying method for Ni content in a carbon material. The assaying method is characterized by comprising the steps of: burning the carbon material with anhydrous sodium carbonate or / and boric acid as a flux, carrying out fusion on ash obtained after burning, leaching with a dilute hydrochloric acid solution to obtain a test solution, reacting nickel and dimethylglyoxime in an alkaline medium in the existence of an oxidant to generate a soluble claret complex, and measuring Ni content by colorimetry. The assaying method for Ni content in the carbon material, provided by the invention, is simple in operation method and high in accuracy.

The invention discloses a hollow copper sulfide / polypyrrole nanocomposite, which has similar peroxidase activity and can generate strong oxidizing hydroxyl radicals by catalyzing H2O2, and can be applied to the detection and analysis of glucose content in food and blood Or the oxidative degradation of organic pollutants in sewage. The nanocomposite of the present invention combines the advantages of copper sulfide and polypyrrole nanomaterials. After compounding, the synergistic catalytic effect of the hollow copper sulfide / polypyrrole nanocomposite is obviously better than that of single copper sulfide particles or polypyrrole particles, and has catalytic High efficiency, short color development time, less usage, stable chemical activity, wide application range, etc., can be used as a substitute for natural peroxidase, used in the detection of glucose content in food and organisms, and in the detection of organic Oxidative degradation of dyes.