36results about How to "Short color development time" patented technology

The invention discloses a method for measuring and analyzing Fe content of a carbon material. The method is characterized by comprising the following steps of: melting the carbon material by using anhydrous sodium carbonate or/and boracic acid as a melting agent, extracting the sample by using a diluted hydrochloric acid solution, reducing Fe (III) in the sample into Fe (II) by using hydroxylamine hydrochloride, regulating the pH value, adding a color development agent, and analyzing and determining the Fe content by using a colorimetric method. The method for measuring and analyzing the Fe content of the carbon material is easy to operate and high in accuracy.

The present invention provides a method of supplementing animals with carotenoids by drinking water, comprising the following steps of: (1) making yellow and/or red pigments into the microencapsulated dry powders or beadlets; (2) mixing the aforementioned microencapsulated dry powders or beadlets in a certain proportion and dissolving them in water to prepare for a pigment solution; (3) administering the prepared pigment solution to animals by drinking. The method of supplementing the carotenoids according to the present invention has the advantages of easy absorption, economical dosages of pigments, high efficiency of coloration, stable coloration and small effects on the process of pigments in comparison with conventional methods currently.

The invention provides a micro-culture method of pleuromutilin producing bacteria. The method comprises the following steps of: culturing the pleuromutilin producing bacteria and then separating a single bacterial colony; preparing a solid fermentation culture medium and sterilizing at a high pressure, and then adding the sterilized solid fermentation culture medium into each pore of an enzyme label plate; inoculating mycelia of the single bacterial colony generated by the pleuromutilin producing bacteria into the enzyme label plate and culturing for 7-9 days. The invention also provides a high-throughput screening method of high-yield bacteria of the pleuromutilin. The high-throughput screening method comprises the following steps of: adding extraction liquid into the pores of the enzymelabel plate of the micro-cultured pleuromutilin producing bacteria to extract; sucking the extraction liquid for each pore and transferring to another enzyme label plate; adding a color-developing agent to develop; measuring by using an enzyme label meter and analyzing the result to obtain the screened high-yield bacteria of the pleuromutilin. As the solid fermentation is adopted, the step of culturing seeds is simplified and the fermentation time is greatly shortened. Compared with the common screening method, the high-throughput screening method has a good related coefficient, thereby beingapplied to screening of the high-yield bacteria of the pleuromutilin.

The invention discloses a feline herpes virus type I gB-gD recombinant protein, and belongs to the field of animal virus antibody detection. The recombinant protein comprises an amino acid sequence asshown in SEQ ID NO.1 or consists of an amino acid sequence as shown in SEQ ID NO.1. The invention further discloses a gene of the recombinant protein. The gene comprises a vector containing the gene,a host cell. The invention also discloses a preparation method of the recombinant protein and application of the recombinant protein in detection of feline herpes virus type I antibody. The recombinant protein is used for detecting the feline herpes virus type I antibody, is convenient and rapid, has high sensitivity, does not have cross reaction with other pathogens, has high specificity, and has huge clinical significance and wide application prospect.

The invention discloses a preparation method of peroxidase-like nano-enzyme beta-FeOOH and application of peroxidase-like nano-enzyme beta-FeOOH in H2O2 detection, and belongs to the technical field of inorganic nano-materials. The peroxidase nano-enzyme beta-FeOOH is obtained through a simple one-step low-temperature hydrolysis precipitation method, H2O2 can be catalyzed to generate hydroxyl freeradicals with high oxidability, and TMB is catalyzed to generate a chromogenic reaction in an extremely short time. Compared with a natural enzyme, the peroxidase-like nano-enzyme beta-FeOOH preparedby the method has the advantages of high stability, easiness in storage and simple process, in addition, the peroxidase-like nano-enzyme beta-FeOOH prepared by the method has higher affinity with H2O2, and is expected to be applied to detection of low-concentration H2O2.

The invention relates to an assaying method for Ni content in a carbon material. The assaying method is characterized by comprising the steps of: burning the carbon material with anhydrous sodium carbonate or/and boric acid as a flux, carrying out fusion on ash obtained after burning, leaching with a dilute hydrochloric acid solution to obtain a test solution, reacting nickel and dimethylglyoxime in an alkaline medium in the existence of an oxidant to generate a soluble claret complex, and measuring Ni content by colorimetry. The assaying method for Ni content in the carbon material, provided by the invention, is simple in operation method and high in accuracy.

The invention discloses a B.canis BcMSA1-BcSA1 recombinant protein, and belongs to the field of animal virus antibody detection. The recombinant protein comprises an amino acid sequence shown in SEQ ID NO. 1 or is composed of the amino acid sequence shown in SEQ ID NO. 1. The invention further discloses a gene of the recombinant protein, a carrier and a host cell containing the gene, a preparationmethod of the recombinant protein and application of the recombinant protein to detection of an antibody of B.canis. The recombinant protein is used for detecting the antibody of B.canis, is convenient and fast to use, high in sensitivity, free of cross reaction with other pathogens and high in specificity, and has great clinical significance and wide application prospects.

The invention belongs to the technical field of medical examination, and particularly relates to a rapid detection kit for bacterial vaginosis, a preparation method and application thereof. The kit comprises a reagent R1 and a reagent R2, and the reagent R1 is composed of 5-bromine-4-chlorine-3-Indolyl alpha-D-N-acetylneuramidate, polyethylene glycol 200 and phosphate buffer solution; and the reagent R2 is composed of 4-amino antipyrine and the phosphate buffer solution. The rapid detection kit for the bacterial vaginosis has the advantages of high determination sensitivity, high accuracy of detection results, good repeatability and high stability, and is an ideal rapid detection kit for the bacterial vaginosis, and the popularization and application of the kit are facilitated.

The invention discloses an RHDV VP10-VP60 recombinant protein, and belongs to the field of animal virus antibody detection. The recombinant protein comprises an amino acid sequence as shown in SEQ IDNO.1 or consists of an amino acid sequence as shown in SEQ ID NO.1. The invention also discloses a preparation method of the recombinant protein. The invention further discloses a gene of the recombinant protein, a vector containing the gene, a host cell containing the gene, a preparation method of the recombinant protein and application of the recombinant protein in an antibody for detecting RHDV. The recombinant protein disclosed by the invention is used for detecting the RHDV antibody, is convenient and rapid, has high sensitivity, does not have cross reaction with other pathogens, has strong specificity, and has huge clinical significance and wide application prospect.

The invention provides a recombinant antigen protein rP44-60 for detecting granulocytoplasmosis and a kit containing the antigen, and belongs to the technical field of immunology, and the recombinant antigen protein comprises an amino acid sequence as shown in SEQ ID NO.1 in a sequence table. The kit comprises a recombinant antigen protein rP44-60, a sample pad, a combination pad, an absorption pad, a nitrocellulose membrane, a colloidal gold probe, mouse IgG and goat anti-mouse IgG. The recombinant antigen protein disclosed by the invention is high in sensitivity and strong in specificity, and can be used for rapidly detecting an antibody in anaplasma phagocytophilum infected serum, so that the problem that the antibody is difficult to capture due to antigen variation of HGA is solved, the defect of existing antigen missing detection is made up, and the missed diagnosis rate is effectively reduced; according to the preparation method, cross reactivity caused by irrelevant sequences is reduced, and the expression quantity and stability of recombinant antigen protein are high; the kit has the characteristics of rapidness, sensitivity, high specificity, no limitation of experimental conditions and the like, and has low requirements on detection conditions.