Total cholesterol detection method

A technology for total cholesterol and detection methods, which is used in biological testing, material testing products, and analysis by chemical reaction of materials, etc. Strong operability and cost-saving effect

Active Publication Date: 2018-07-27
QINGDAO HIGHTOP BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And a method for preparing whole blood total cholesterol quality control is proposed, which is more conducive to product testing and overcomes the current difficulty in purchasing whole blood / serum quality control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0035] 1) Preparation of detection reagent and test paper:

[0036] 1~2g / L trehalose, 0.2~0.4g / L bovine serum albumin, 0.1~1mmol / L 4-aminoantipyrine, 3~5mmol / L sodium cholate, 0.1~1mmol / L EDTA, 3 ~4mmol / L chromogen, 8~10KU / L cholesterol esterase, 4~8KU / L cholesterol oxidase, 10~20KU / L peroxidase, 1~3g / L TritonX-100, 4~8KU / L Ascorbate oxidase and 4-6mmol / L magnesium chloride are dissolved in phosphate buffer (pH=6-8, 0.01M-0.2M), soaked with test paper, placed at 40-50°C and dried.

[0037]The test paper includes a perforated PVC plate I1, a blood filter membrane 2, a reaction membrane 3 and a perforated PVC plate II4 from top to bottom, the upper end of the perforated PVC plate I1 is provided with a sampling hole 6, and the perforated PVC plate II4 Corresponding to the sample injection hole 6, there is an observation hole 7; the perforated PVC plate I1 and the perforated PVC plate II4 sandwich the blood filtration membrane 2 and the reaction membrane 3, and the blood filtrati...

Embodiment 1

[0049] A method for detecting total cholesterol, comprising the steps of:

[0050] 1) Preparation of detection reagent and test paper:

[0051] 1g / L trehalose, 0.2g / L bovine serum albumin, 1mmol / L 4-aminoantipyrine, 5mmol / L sodium cholate, 1mmol / L EDTA, 3mmol / L chromogen, 8KU / L cholesterol Esterase, 4KU / L cholesterol oxidase, 10KU / L peroxidase, 3g / L TritonX-100, 8KU / L ascorbate oxidase, 6mmol / L magnesium chloride, use phosphate buffer (pH=6~8, 0.01 M~0.2M) to dissolve, infiltrate with test paper and place it to dry at 40~50℃.

[0052] The test paper includes a perforated PVC plate I1, a blood filter membrane 2, a reaction membrane 3 and a perforated PVC plate II4 from top to bottom, the upper end of the perforated PVC plate I1 is provided with a sampling hole 6, and the perforated PVC plate II4 Corresponding to the sample injection hole 6, there is an observation hole 7; the perforated PVC plate I1 and the perforated PVC plate II4 sandwich the blood filtration membrane 2 and...

Embodiment 2

[0059] A method for detecting total cholesterol, comprising the steps of:

[0060] 1) Preparation of detection reagent and test paper:

[0061] 2g / L trehalose, 0.4g / L bovine serum albumin, 1mmol / L 4-aminoantipyrine, 3mmol / L sodium cholate, 0.1mmol / L EDTA, 4mmol / L chromogen, 10KU / L Cholesterol esterase, 8KU / L cholesterol oxidase, 20KU / L peroxidase, 1g / L TritonX-100, 4KU / L ascorbate oxidase, 4mmol / L magnesium chloride, use phosphate buffer (pH=6~8, 0.01M~0.2M) to dissolve, infiltrate with test paper, place it at 40~50℃ and dry it.

[0062] The test paper includes a perforated PVC plate I, a blood filter membrane, a reaction membrane and a perforated PVC plate II from top to bottom, and a sample injection hole is provided on the upper end of the perforated PVC plate I, and the perforated PVC plate II is connected to the injection hole. Corresponding to the sample hole, there is an observation hole; the perforated PVC plate Ⅰ and the perforated PVC plate Ⅱ sandwich the blood fil...

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Abstract

A total cholesterol detection method comprises steps as follows: 1) a detection reagent and a test strip thereof are prepared; 2) total cholesterol whole blood control products with different concentrations are prepared; 3) the total cholesterol whole blood control products with different concentrations in the step 2) are dropwise added to the test strip obtained in the step 1), the test strip displays different colors, total cholesterol concentration detection is performed on the total cholesterol whole blood control products with different concentrations by use of a full-automatic biochemical analyzer, the test strip color developing results are calibrated according to detection results of the total cholesterol whole blood control products with the same concentration by use of the full-automatic biochemical analyzer, and a colourimetric card is obtained; 4) when the test strip obtained in the step 1) is used for detecting the total cholesterol concentration of a sample, the sample isdropwise added to the test strip, the color developing result of the test strip is compared with the colourimetric card in the step 3) after 2-3 min, and the concentration of the total cholesterol inthe sample is obtained. The cost and the time can be saved, and the stability, repeatability and sensitivity of the method can be guaranteed.

Description

technical field [0001] The invention belongs to the field of medical test strips for in vitro detection, and in particular relates to a method for detecting total cholesterol. Background technique [0002] In recent years, with the development of my country's economy, the improvement of residents' living standards and the acceleration of population aging, cardiovascular disease has become the number one cause of death in my country's urban and rural population. The detection of blood lipids has important guiding significance for the diagnosis of cardiovascular diseases. As one of the detection indicators of clinical blood lipid items, total cholesterol has attracted the attention of researchers. Cholesterol, also known as cholesterol, is a derivative of cyclopentane polyhydrophenanthrene. Total cholesterol refers to the sum of cholesterol contained in all lipoproteins in the blood. Total cholesterol includes free cholesterol and cholesteryl esters, and the liver is the mai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/92
CPCG01N21/78G01N33/92
Inventor 杨帆李俊美张小蓬刘太益宋金玲王新鹏
Owner QINGDAO HIGHTOP BIOTECH
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