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Preparation method of peroxidase-like nano-enzyme beta-FeOOH and application of peroxidase-like nano-enzyme beta-FeOOH in H2O2 detection

A peroxide and nano-enzyme technology, which is applied in metal/metal oxide/metal hydroxide catalysts, chemical instruments and methods, and analysis by making materials undergo chemical reactions, can solve the problem of hydrogen peroxide simulating enzyme reaction efficiency Low-level problems, to achieve the effect of accurate measurement results

Active Publication Date: 2020-11-20
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to overcome the shortcoming of above-mentioned prior art, the object of the present invention is to provide a kind of preparation method of peroxide nanozyme β-FeOOH and its reaction in H 2 o 2 Application in detection to solve the technical problem of low efficiency of the existing hydrogen peroxide mimic enzyme reaction

Method used

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  • Preparation method of peroxidase-like nano-enzyme beta-FeOOH and application of peroxidase-like nano-enzyme beta-FeOOH in H2O2 detection
  • Preparation method of peroxidase-like nano-enzyme beta-FeOOH and application of peroxidase-like nano-enzyme beta-FeOOH in H2O2 detection
  • Preparation method of peroxidase-like nano-enzyme beta-FeOOH and application of peroxidase-like nano-enzyme beta-FeOOH in H2O2 detection

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Experimental program
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Effect test

Embodiment 1

[0029] Peroxide-like nanozyme β-FeOOH is a common natural geological mineral with abundant content, non-toxic and harmless. This mineral is often used to prepare Fe 3 o 4 and Fe 2 o 3 The precursor of the peroxide-like nanozyme β-FeOOH has a strong affinity for heavy metals and can be used to remove heavy metal pollutants in water systems. The invention prepares a peroxide-like nanozyme β-FeOOH with high specific surface area, good stability and abundant hydroxyl groups through a new preparation method, and uses it as a catalyst.

[0030] In this example, 20 mmol of ferric chloride solid was added into 100 ml of a polyethyleneimine aqueous solution with a mass concentration of 0.1% for thorough stirring, mixed and reacted at 80° C. for 2 h, and washed alternately with deionized water and ethanol three times. After washing, centrifugal concentration is carried out, so that the final concentration of the peroxide-like nanozyme β-FeOOH aqueous solution is 10 mg / mL, and the pe...

Embodiment 2

[0033] Take 20 μL of the peroxide-like nanozyme β-FeOOH solution prepared in Example 1, mix it with 200 μL, 20 mM TMB, and add a volume of 6 μL of H 2 o 2 , and finally use acetic acid-sodium acetate buffer solution with pH 4.0 to make up to 3mL, so that H 2 o 2 The final concentration range was 0.1 mM. After reacting for 5min, use the ultraviolet-visible-near-infrared spectrometer to measure the absorbance at 652nm, and calculate H at different moments in the reaction process according to formula (1). 2 o 2 The concentration C,

[0034]

[0035] Among them, A is the absorbance, ε TMBox It is the molar absorptivity coefficient, which is related to the nature of the absorbing substance and the wavelength λ of the incident light. C is the concentration of the light-absorbing substance in mol / L, and L is the thickness of the absorbing layer in cm.

[0036] Then within 0 to 5 minutes of the mixed reaction, measure the absorbance of the mixed solution at different reactio...

Embodiment 3

[0041] Take 30 μL of the peroxide-like nanozyme β-FeOOH solution prepared above and mix it with 20 mM TMB, add 6 μL, 12 μL, 24 μL, 36 μL, 48 μL, 60 μL of H 2 o 2 , and finally use 0.02M acetic acid-sodium acetate buffer solution with pH 5.8 to make up to 3mL, so that H 2 o 2 The final concentrations correspond to 0.1 mM, 0.2 mM, 0.4 mM, 0.6 mM, 0.8 mM, and 1 mM, respectively. The absorbance at 652nm was measured at different reaction times of the mixed solution using a UV-Vis-NIR spectrometer to obtain H 2 o 2 Concentration and the relation of reaction rate, carry out fitting graph with Michaelis equation (Michealis-Menten equation), the result is as follows image 3 shown. The Michaelis equation is as follows:

[0042] Michealis-Menten curve

[0043] according to image 3 The Mie equation obtained to discuss the β-FeOOH vs. H 2 o 2 According to the above-mentioned Michealis-Menten equation, the peroxide-like nanozyme β-FeOOH prepared by the present invention is ca...

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Abstract

The invention discloses a preparation method of peroxidase-like nano-enzyme beta-FeOOH and application of peroxidase-like nano-enzyme beta-FeOOH in H2O2 detection, and belongs to the technical field of inorganic nano-materials. The peroxidase nano-enzyme beta-FeOOH is obtained through a simple one-step low-temperature hydrolysis precipitation method, H2O2 can be catalyzed to generate hydroxyl freeradicals with high oxidability, and TMB is catalyzed to generate a chromogenic reaction in an extremely short time. Compared with a natural enzyme, the peroxidase-like nano-enzyme beta-FeOOH preparedby the method has the advantages of high stability, easiness in storage and simple process, in addition, the peroxidase-like nano-enzyme beta-FeOOH prepared by the method has higher affinity with H2O2, and is expected to be applied to detection of low-concentration H2O2.

Description

technical field [0001] The invention belongs to the technical field of inorganic nanomaterials, and relates to a preparation method of peroxide-like nanozyme β-FeOOH and its reaction in H 2 o 2 application in detection. Background technique [0002] Natural catalase is a kind of protein with catalytic function. A small amount of it can greatly speed up the reaction speed, and it does not change before and after the reaction. It is often used in the detection of food, fabrics and bacteria and the treatment of water pollutants. However, natural enzymes are volatile and inactivated, expensive, difficult to purify, and costly to store and use, making the study of mimic enzymes a top priority. [0003] The classic Fenton reaction can generate a large number of hydroxyl radicals with strong oxidizing ability, and has been widely used in wastewater treatment. But this method needs Fe 2+ As a consumable, a large amount of iron sludge is produced, causing subsequent disposal prob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N21/78B01J23/745
CPCG01N21/359G01N21/78B01J23/745
Inventor 杨冬雷蕾高可奕
Owner SHAANXI UNIV OF SCI & TECH
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