71 results about "Heavy chain gene" patented technology

A human artificial chromosome vector comprising a human antibody heavy chain gene, a human antibody light chain gene, and a human antibody surrogate light chain gene.

The invention relates to a silkworm fibroin heavy-chain gene mutation method, which specifically comprises the step of: acting mRNA (Messenger Ribonucleic Acid) of a coded zinc-finger nuclease sequence on loci 1325-1362 of a silkworm fibroin heavy-chain gene shown as SEQ ID NO:1 to form target positions for recognizing zinc-finger nuclease, thereby obtaining a series of silkworm fibroin heavy-chain mutated genes; and the mutated sequence can be applied to preparation of sericin and extrinsic proteins. Mutants provided by the invention have the following advantages that: (1) a posterior division of silkgland of a fibroin heavy-chain gene mutant provided by the invention serious degrades, a cocoon shell only contains the sericin synthesized and secreted by a middle division of silkgland, if the mutated strains are utilized to transgenically express the extrinsic proteins, the expressed amount and the purity of the extrinsic proteins can be greatly increased, and thus, a brand-new useful genetic material is provided for the development of a silkworm fibroin bioreactor; and (2) the cocoon shell of the mutated strain provided by the invention only contains the sericin, and thus, a new source is provided for the large-scale production of the sericin.

Belonging to the field of biotechnology, the invention relates to fully human monoclonal antibodies against Zika virus, antigen binding fragments and application thereof. The antibodies provided by the invention are determined by complementary determining region (CDR) specific gene sequences in antibody light chain and heavy chain gene variable regions and are antibodies specifically bound to Zikavirus envelope glycoprotein E (protein E) and effectively expressed in prokaryotic and eukaryotic cells. The antibody CDR region or partial or whole genes can be utilized to transform and produce genetic engineering antibodies of different forms in prokaryotic and eukaryotic cells and any expression system, and can prevent or treat Zika virus related diseases clinically.

The invention relates to a human body beta-amyloid protein detection kit and application thereof. The human body beta-amyloid protein detection kit is prepared by the following steps: firstly, immunizing a mouse by use of antigen beta-amyloid precursor protein, separating out obvious lymph nodes in the spleen and the body of the mouse after confirming successful immunization, extracting total RNAs (Ribose Nucleic Acids), performing reverse transcription on the total RNAs to obtain cDNAs (complementary Desoxvribose Nucleic Acids), and amplifying the light and heavy chain genes of an antibody by virtue of PCR (Polymerase Chain Reaction); secondly, assembling and amplifying scFvDNA and constructing pCANTAB5E-scFv recombinant plasmid; thirdly, converting the plasmid to obtain a single-chain antibody phage library, and screening out to obtain target antibodies; and finally, assembling the target antibodies to form the human body beta-amyloid protein detection kit. The detection range of the screened antibodies is relatively wide from 0 to 1,000pg / ml, the linear correlation coefficient of the kit is high, the detection sensitivity of the kit can be 3.5pg / ml, and the kit achieves high stability; during detection, the reaction patterns of a two-step method and a room temperature oscillating method can be adopted, with low requirements on the laboratory conditions and the equipment.

The invention discloses a bombyx mori silk heart protein heavy-chain expression system with expression protein distributed on a bombyx mori silk glue layer and a preparation method and an applicationthereof, the bombyx mori silk heart protein heavy-chain expression system contains a target gene expression cassette, and the 5'end of the expression cassette is a signal peptide sequence of a bombyxmori silk heart protein heavy chain promoter 3; the 3'end is a heavy chain gene poly (A) sequence; when the system is used for expressing the target protein, the target protein only exists in a sericin layer and is not expressed in a silk fibroin layer, so that the system can be used for expressing the target protein in the sericin layer, the creation of a real and practical bombyx mori silk glandbioreactor is facilitated, and the long-term development of silkworm science and insect discipline in China is promoted; and experimental and theoretical bases are provided for the silk forming mechanism of the silk.

The invention discloses a single-chain antibody of an anti-schistosome monoclonal NP11-4 as well as a preparation method and application thereof, and relates to the field of gene engineering and the field of therapeutic drugs against schistosomiasis. The invention is to use a monoclonal antibody NP11-4 located at adult membrane, cercaria memberane, schistosomulum membrane, egg shell and miracidium in egg of Schistosoma japonicum, extract total RNA of hybridoma cells through a gene engineering technology, adopt RT-PCR for proliferation of genes VH and VL, and use overlap-extension PCR to connect the genes VH and VL into a single-chain antibody gene in the form of VH<-linker-VL, which is subsequently connected with a pBAD / gIIIA carrier and cloned into Top10F'of Escherichia Coli to induce a soluble expression of the target gene. The zone from amino acid No.1 to No. 122 of the expressed single-chain antibody of the anti-schistosome monoclonal NP11-4 is a heavy chain gene repertoire of the single-chain antibody (VH), and the zone from the amino acid No.141 to No.254 is a light chain gene repertoire (VL) of the single-chain antibody, wherein the linker between the heavy and light chain gene repertoires consists of 18 amino acids including repeated glycines and serines, and the single-chain antibody has 254 amino acids in full length.

The invention discloses a human-derived anti-amylin antibody and a preparation method thereof, and belongs to the field of immune globulin. The heavy chain variable region amino acid sequence of the antibody is shown as SEQ ID NO:1, the light chain variable region amino acid sequence of the antibody is shown as SEQ ID NO:2, the heavy chain variable region DNA sequence of the antibody is shown as SEQ ID NO:3, and the light chain variable region DNA sequence of the antibody is shown as SEQ ID NO:4. The heavy chain gene is an IgG1 subclass VH3 gene family, and the light chain gene is a Lambda gene family. The human-derived amylin antibody is screened out by establishing a high-capacity phage antibacterial library, the antibody has good antigen specificity and antigen combination activity, production of an amylin detecting kit is guaranteed, and wide application prospects are achieved.

The invention discloses a monoclonal antibody resisting EV-D68 virus and preparation and application thereof. A single B cell separation technique is firstly adopted to conduct separation of the specific single memory B cell on a rhesus monkey infected by EV-D68 virus, paired antibody light and heavy chain genes are obtained, and the EV-D68 specific monoclonal antibody and the neutralizing activity can be obtained through further screening. The monoclonal antibody comprises a heavy chain and a light chain, and the nucleotide coding sequence and the amino acid coding sequence of the monoclonalantibody are as shown in the sequence graph. The invention provides an alternative drug for preventing and curing infection of EV-D68, and provides a new tool and new idea for identifying the EV-D68 virus.