Fully human monoclonal antibodies against Zika virus and application thereof

A monoclonal antibody and Zika virus technology, applied in the fields of application, antibody, antiviral agent, etc., can solve the problems of no specific therapeutic drugs and no Zika virus vaccine developed

Inactive Publication Date: 2018-01-16
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, more and more studies have confirmed that Zika virus is related to fetal and neonatal microcephaly, a rare autoimmune disease Guillain-Barre syndrome (Guillain-Barre syndrome, GBS) and other diseases. An effective vaccine against Zika virus has been developed, but there is no specific therapeutic drug

Method used

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  • Fully human monoclonal antibodies against Zika virus and application thereof
  • Fully human monoclonal antibodies against Zika virus and application thereof
  • Fully human monoclonal antibodies against Zika virus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1) Production of Zika virus E protein

[0062] The synthetic Zika virus E protein gene was inserted into the expression plasmid, and the expression plasmid was transiently transfected into Expi293 cells, and the E protein-human Fc fusion protein was expressed and purified;

[0063] 2) Screening of Zika virus-specific antigen-binding fragments (Fab)

[0064] Using a large phage display antibody library constructed from the cDNA of peripheral blood mononuclear cells from 40 healthy volunteers, antibodies were screened against biotinylated protein E; biotinylated protein E was immobilized on streptavidin-coated magnetic beads up, 10 12 Each phage-displayed Fab was incubated with 5, 4, 2, and 1 micrograms of antigen for two hours in rounds 1, 2, 3, and 4 at room temperature, and 10 phages were used for each round of screening. 12 1; use polyclonal phage ELISA to detect the enrichment of antibodies, the 1st, 2nd, 3rd, and 4th rounds of phages are incubated with coated prot...

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Abstract

Belonging to the field of biotechnology, the invention relates to fully human monoclonal antibodies against Zika virus, antigen binding fragments and application thereof. The antibodies provided by the invention are determined by complementary determining region (CDR) specific gene sequences in antibody light chain and heavy chain gene variable regions and are antibodies specifically bound to Zikavirus envelope glycoprotein E (protein E) and effectively expressed in prokaryotic and eukaryotic cells. The antibody CDR region or partial or whole genes can be utilized to transform and produce genetic engineering antibodies of different forms in prokaryotic and eukaryotic cells and any expression system, and can prevent or treat Zika virus related diseases clinically.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a fully human monoclonal antibody against Zika virus, an antigen-binding fragment thereof, and applications thereof. Background technique [0002] The prior art discloses that Zika virus is an arbovirus, which was first found in rhesus monkeys in the Zika jungle in Uganda in 1947, and belongs to the genus Flavivirus (flavivirus) like dengue fever, flavivirus and West Nile virus. division). For a long time, Zika virus has been ubiquitous in tropical areas of African and Asian countries, but it cannot cause widespread infection in the human population. Until 2007, an outbreak of Zika virus occurred on Yapu Island in the Micronesia Islands in the Western Pacific, followed by a larger outbreak in French Polynesia in Oceania in 2013-2014, infecting about 32,000 people . In 2015, the Zika virus reached Brazil, causing nearly 30,000 infections, and then quickly spread to other...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C07K16/46C12N15/13C12N15/85C12N5/10G01N33/68G01N33/577G01N33/569A61K39/42A61K38/16A61P31/14
CPCY02A50/30
Inventor 应天雷姜世勃吴艳玲周辰
Owner FUDAN UNIV
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