79 results about "Chlorophyll synthesis" patented technology

The invention discloses a strawberry seedling substrate, and a production method and application thereof. The substrate comprises the following components: earthworm cast, peat, perlite and vermiculite, wherein the volume ratio of earthworm cast, peat, perlite to vermiculite is (1-4):(0-4):1:1; the earthworm cast is a product formed by earthworms taking cow dung. According to the strawberry seedling substrate, and the production method and application thereof, peat is replaced with the earthworm cast obtained by raising the earthworms fed with cow dung, so that resourceful utilization channels of the earthworm cast are expanded, environmental pollution is reduced, the ecological environment is protected, and the recycling and low-carbon circulation of resources are realized; adequate nutrients can be provided for strawberries in a seedling period, chlorophyll synthesis can be promoted, and the activity of peroxidase in strawberry leaves can be improved; the rooting of maternal seedlings and the growth of creeping stems are promoted, the strawberry seedling quality is improved, the rooting rate is increased, high survival rate and seedling uniformity in the seedling period are ensured, the number of strong seedlings is remarkably increased, seedling lifting labor is saved, and convenience in operation and transportation is ensured.

The invention relates to the technical field of biology, and discloses a coenzyme-Q10-production engineered bacteria construction method, engineered bacteria, and an application thereof. The method comprises the steps that: a, total genomic DNA is extracted from Rhodobacter sphaeroides cacterial liquid; b, bchG homologous gene is obtained by amplification with a polymerase chain reaction; c, the amplified homologous gene is connected with broad-host plasmid, such that recombinant vector is constructed; d, the recombinant vector is transferred to Escherichia coli S17-1; and e, the Escherichia coli S17-1 is subjected to conjugal transfer with the Rhodobacter sphaeroides, such that the engineered bacteria with chlorophyll synthesis gene bchG knocked out are obtained. According to the method provided by the invention for improving coenzyme Q10 yield through knocking out Rhodobacter sphaeroides chlorophyll synthesis gene, coenzyme Q10 synthesis capacity can be improved by higher than approximately 15%. The method is suitable for coenzyme Q10 large-scale industrial production.

The invention discloses an acid-resistant pseudomonas koreensis strain CLP-7 which is capable of preventing diseases and promoting growth, and can be used for biocontrol. The acid-resistant pseudomonas koreensis strain CLP-7 is preserved at China General Microbiological Culture Collection Center, on 27th, October, 2016, and the preservation number is CGMCC No.13204. The antibacterial spectrum of the acid-resistant pseudomonas koreensis strain CLP-7 is relatively large; the acid-resistant pseudomonas koreensis strain CLP-7 is capable of inhibiting growth of Phytophthora parasitica var. nicotianae, Ralstonia solanacearum, and Alternaria alternata Keissler; the antagonistic activity under acidic conditions is high, the acid-resistant pseudomonas koreensis strain CLP-7 is capable of producing siderophore, possesses protease and glucanase activity, and potassium releasing capacity. The results of biocontrol pot experiment show that the acid-resistant pseudomonas koreensis strain CLP-7 is capable of preventing under acidic soil conditions, and promoting growth and chlorophyll synthesis of tobacco seedlings in acidic soil, so that the acid-resistant pseudomonas koreensis strain CLP-7 and microorganism bacterium agents of the acid-resistant pseudomonas koreensis strain CLP-7 can be used for preventing tobacco fungi and bacterial root and stem diseases under continuous cropping or acidic soil conditions effectively, and is high in application value.

The invention provides a formulation method of a green lawnger. The green lawnger is characterized by comprising the following components by weight percent: 0.03-0.05 part of potassium dihydrogen phosphate, 0.03-0.05 part of potassium nitrate, 0.05-0.1 part of sodium nitrate, 0.01-0.03 part of EDTA-Fe, 0.05-0.1 part of EDTA-Mg, 0.02-0.05 part of sodium naphthalene acetate and 0.01-0.05 part of manganese sulfate in 100 parts of deionized water. According to the green lawnger, a leaf surface spraying mode is adopted to provide nutrients for a lawn in order to enhance the growth vitality of the lawn and improve the stress resistance of the lawn. The green lawnger has the advantages that the nutrients are absorbed through the leaf surfaces so that the effect is quickly achieved; the green lawnger contains macroelements such as nitrogen, phosphorus and potassium as well as trace elements such as ferrum, magnesium, manganese and the like which directly act on leaves to promote chlorophyll synthesis and recover green of leaves, therefore, the problem that the lawn is withered and yellow is thoroughly solved.

The invention relates to a gene for improving chlorophyll synthesis and photosynthesis efficiency of tomatoes and application. The gene for improving the chlorophyll synthesis and photosynthesis efficiency of the tomatoes is composed of a tomato S1HY5 gene and protein encoded by the gene. The invention provides the application of the tomato S1HY5 gene or the protein encoded by the tomato SlHY5 gene to the improvement of chlorophyll synthesis of tomato plants and promotion of the photosynthesis of tomato plant leaves and a method for obtaining the transgenic tomato plants with the increased plant chlorophyll and photosynthesis rate. According to the specific method, through a gene overexpression technology, the expression level of the tomato S1HY5 gene is increased, and therefore the chlorophyll of the tomato plants is increased; the functions of increasing the chlorophyll content of the tomatoes and improving the photosynthesis efficiency of the tomatoes are determined, a gene resourceis provided for cultivating the new tomato varieties with the high chlorophyll content, the gene has high application value, and a theoretical basis is laid for a regulation mechanism of optical signals for the chlorophyll synthesis and photosynthesis of the plants.

The invention discloses a chlorophyll synthesis related protein, a coding gene and application thereof. The protein provided by the invention is a) or b) as follows: a) a protein composed of an amino acid sequence shown as sequence 1 in a sequence table; b) a chlorophyll synthesis related protein obtained by subjecting the amino acid sequence of the a) defined protein to substitution and / or deletion and / or adding by one or several amino acid residues. Experiments prove that a transgenic plant can be obtained by inhibiting chlorophyll synthesis related gene ALAD through artificial microRNA (amiRNA), and the transgenic plant shows constitutive disease resistance response. Therefore, artificial inhibition of ALAD gene expression provides a brand new and effective way for genetic improvement of crops' resistance to diseases.

The invention discloses a functional foliar fertilizer and a preparation method thereof. The functional foliar fertilizer is prepared from urea, sodium selenite, disodium octaborate tetrahydrate, potassium dihydrogen phosphate, fulvic acid, trace elements, garlic polysaccharide, pullulan, polyglutamic acid, ethylenediamine tetraacetic acid disodium salt, naphthylacetic acid, a zinc ion-porphyrin nanocomplex and water. The functional foliar fertilizer provided by the invention has the excellent performance of high efficiency, environmental protection, seedling strengthening, stress resistance and insect pest resistance, can quickly penetrate into plants after contact, and promote cell growth and division, and synthesis of nucleic acid and protein, delay organ abscission and crop senescenceas well as chlorophyllin synthesis, increase the photosynthetic rate and enhance the stress resistance, break apical dominance, and promote the robust rather than flourishing growth of plants, not only increases the total nutrient synthesis, but also coordinates the distribution relation of nutrition and reproductive growth, and greatly improves the crop yield and quality.

The invention discloses a modifier for increasing production of corns and rice, which contains forchlorfenuron, gibberellin and salicylic acid and the like. The medicaments are uniformly mixed according to a certain ratio under a room temperature drying environment. An increasing agent solution is sprayed in a later stage of flowering of rice and corns or a filling stage. The modifier can promote cell growth and division and the synthesis of nucleic acid and protein, and delay the organ abscission, crop senescence and chlorophyll synthesis. The photosynthetic rate is improved and the stress resistance is enhanced. The apical dominance is broken, the fruit setting is promoted and the fruits are amplified, and the parthenocarpy is induced, so that the crops are matured for 7-10 days in advance. The yields of rice and corns are increased by above 10%. The modifier is nontoxic and harmless, safe and reliable, low in cost, convenient to use and easy to operate.

A composition for reducing the content of nitrate in a cultivation plant, characterized in that the composition comprises chitosan, an organic acid, a nitrate reductase activator, and a chlorophyll synthesis promoter, in the following mass ratio (%): chitosan 15 to 25 organic acid 15 to 25 nitrate reductase activator 25 to 30 chlorophyll synthesis promoter 29 to 35.

The invention discloses corn yield improvement accelerant. The corn yield improvement accelerant is composed of zhongshengmycin, cyprosulfamide, cysteine, plant growth regulator agents, zeatin, 2,4-dichlorobenzene oxygen butyl acetate, nanometer titania, abamectin and alginic acid. The corn yield improvement accelerant is a compound and efficient type accelerant, the proportion is reasonable, the accelerant has the great functions of promoting cell enlargement and division, promoting synthesis of nucleic acid and protein, suspending organ abscission, crop aging and chlorophyll synthesis, improving photosynthetic rate, enhancing the stress resistance, breaking apical dominance, promoting fruit setting, enlarging the fruits and inducing parthenocarpy, the cob rate is high, cobs are big, the grains are starlatine, and under the normal growth environment, the yield per mu is increased by 250 kilograms at the minimum. The corn yield improvement accelerant has a great effect for preventing and treating field diseases caused by bacteria and fungus, the chemical dosage is reduced, the chemical cost is reduced, no residues exist, the accelerant is convenient to use, the operation and implementation are easy, the dosage is little, and the cost is low.

The invention discloses a protein for adjusting rice chlorophyll synthesis and an encoding gene and application thereof. The protein has an amino acid sequence as shown in SEQ ID NO: 3. The encoding gene of the protein has a nucleotide sequence as shown in SEQ ID NO: 1. The mutation of the encoding gene of the protein related to the rice chlorophyll precursor synthesis leads to yellow stripe leaves of the rice plants, and severe mutation leads to death of whiten seedlings. The gene in a normal planting is knocked out through a Crisp / cas9 gene encoding technology, and the normal green plant phenotype can be mutated into whiten seedlings and dies after trefoil stage. The encoding gene is an important indicating gene in the works such as the work of genetic improvement of the plant, and theencoding gene can be treated as a target gene to be applied to the seed production of hybrid rice; particularly the encoding gene can rapidly conveniently detect the purity of filial generation F1 inthe seedling stage during the seed production of two-line system hybrid rice; and meanwhile, the carried target gene sterile line etiolated seedling can be quickly removed in the seedling stage duringplanting the hybrid rice.

The invention discloses a method for preparing a special leaf fertilizer for mangoes from ageratina adenophora. The method comprises the steps of crushing aboveground stalks of purple ageratina adenophora, conveying the crushed stalks into an extracting tank, firstly, extracting with water, and then, filtering to obtain a water extraction solution; extracting filter residues by using ethanol, and filtering after extracting to obtain an ethanol extraction solution; and mixing the water extraction solution and the ethanol extraction solution, and adding a certain quantity of ethephon, trace elements, humic acid and monopotassium phosphate into the mixed solution to obtain the special leaf fertilizer for mangoes. By using the leaf fertilizer prepared by using the method disclosed by the invention, the chlorophyll synthesis of leaves can be promoted, the photosynthesis of the leaves can be enhanced, the flower bud differentiation of mango branches can be promoted, bacterial diseases, fungal diseases and pests can be prevented and controlled, the pistillate flower ratio of mango flowering branches can be increased, the amount of middle-sized fruits can be increased, huge fruits can be avoided, the yield of the mangoes can be increased, and the quality of the mangoes can be improved.

The invention relates to a molecular marker in the technical field of muskmelon genetic engineering, and in particular relates to a pair of molecular markers which are used for positioning a muskmelonyellow and green leaf color gene ygl, and are tightly interlocked with the muskmelon yellow and green leaf color gene ygl. When the molecular marker Indel24 is used for positioning the yellow and green leaf color gene ygl, PCR (Polymerase Chain Reaction) amplification can be carried to obtain a characteristic strip which is tightly interlocked with 191 bp; when the molecular marker Indel43 is used for positioning the yellow and green leaf color gene ygl, PCR amplification can be carried to obtain a characteristic strip which is tightly interlocked with 200 bp. The yellow and green leaf colorgene ygl is located between the two molecular markers, and a physical distance is only about 26Kb. By utilizing the result, a physical map between the two molecular markers can be obtained, so that afoundation is laid for final cloning of the gene ygl and establishment of a molecular marker assisted breeding system; meanwhile, a foundation is also laid for development of muskmelon chloroplasts and researches of a chlorophyll synthesis regulation and control network.

The invention relates to a foliar fertilizer assisting in comprehensively supplementing nutrients needed by crop growth, regulating better growth of crops and substantially improving crop yield. With added rare earth elements lanthanum and cerium, seed germination can be promoted, root system growth can be promoted, root system vitality can be improved, crop stem growth can be promoted, seedling dry weight and chlorophyll content can be improved, crop photosynthetic efficiency can be improved, and further crop yield and quality can be improved. A trace element selenium is added, and can act synergistically with gibberellin, quaternary ammonium salt chitosan and the like, such that fungus inhibition capacity is improved, crop resistance is improved, chlorophyll synthesis can be regulated, and crop quality can be improved. A main component modified quaternary ammonium salt chitosan has relatively high repelling and inhibition effects against crop pests and diseases, and can be used for replacing chemical insecticide or fungistat with toxic or side effect in traditional foliar fertilizers. Also, the quaternary ammonium salt chitosan can naturally degrade, and has an advantage of low dose. Compared with that of traditional foliar fertilizers, the cost of the fertilizer provided by the invention is reduced by approximately 10%. The fertilizer has the three advantages of high efficiency, environment friendliness, and low cost.

The invention discloses a planting method of gentiana macrophylla and belongs to the field of Chinese herbal medicine planting. The planting method includes: (1), selecting and preparing land; (2), seeding and cultivating seedlings; (3), thinning the seedlings; (4), transplanting; (5), performing field management, wherein potassium fertilizer, phosphorus fertilizer and foliage fertilizer are additionally applied during field management, and the foliage fertilizer comprises 0.1-0.15% of organic selenium, 0.5-1% of pyrophosphoric acid and 1-3% of amino acid compound liquid. In the planting method, the phosphorus fertilizer, the potassium fertilizer and farm manure are additionally applied, and a specific proportion is adopted, so that development of gentiana macrophylla rhizome can be improved, and yield of gentiana macrophylla can be increased; nutritional ingredients are provided for synthesis of gentiopicroside in gentiana macrophylla, content of gentiopicroside is increased, so thatmedicinal effect is improved. The foliage fertilizer contains organic selenium and pyrophosphoric acid, organic selenium can promote cell division and improve photosynthesis and chlorophyll synthesis,so that synthetic content of gentiopicroside is increased, and the medicinal effect of gentiana macrophylla is improved.

The invention relates to a strategy for growth and metabolism regulation of microalgae cultured by urban sewage. The strategy mainly includes: (1) in an early culture stage, a plant growth regulator and COD (chemical oxygen demand) degrading bacteria are added into microalgae-containing urban sewage to promote chlorophyll synthesis and degradation of organics in sewage and improve microalgae cellphotosynthetic efficiency and carbon sequestration efficiency to increase biomass yield; (2) in a later culture stage, the nitrogen content of sewage meets a nitrogen deficiency condition, and accordingly a glycolytic pathway is regulated so as to promote carbohydrate metabolism to enable more carbon for fatty acid synthesis, and grease accumulation promotion is realized. Adopting the urban sewagefor culturing microalgae meets the requirement of a two-stage culture process in a same system, both microalgae high-density culture and grease accumulation can be achieved, simplicity and convenience in operation are achieved, and large-scale production is facilitated; the strategy is significant to guidance of large-scale microalgae culture and provides theoretical and technical support for process optimization of an industrial chain for preparing biodiesel from microalgae cultured by urban sewage.

The invention discloses a rice yellow green leaf trait gene ygl8 and application thereof to rice breeding and belongs to the field of genetic breeding. The nucleotide sequence of the rice yellow green leaf trait gene ygl8 is as shown in Seq ID NO.1, and the nucleotide sequence of a wild type gene YGL8 corresponding to the rice yellow green leaf trait gene ygl8 is as shown in Seq ID NO.2; coded protein of the wild type gene YGL8 is key enzyme, namely magnesium protoporphyrin IX methyl transferase, in the chlorophyll synthesis pathway, and the nucleotide sequence of the coded protein is as shown in Seq ID NO.3. The ygl8 gene has wide application prospect in hybridization F1-generation hybrid purity identification work and sterile line self-fertilized breed conservation purity identification work of rice breeding.

The invention belongs to the technical field of vegetable insect prevention and particularly relates to a vegetable insecticide and a preparation method thereof. The vegetable insecticide is prepared from raw materials in parts by weight as follows: 10-15 parts of camellia assamica meal, 10-15 parts of phoebe bournei, 10-15 parts of michelia foveolata, 10-15 parts of magnolia grandiflora, 6-10 parts of sodium nitroprusside, 4-8 parts of a penetrant and 4-8 parts of a modified starch solution. The vegetable insecticide is prepared from natural plants and so has the advantages of being healthy, non-toxic and pollution-free, and the vegetable insecticide has a good insecticidal effect, has the effect of promoting chlorophyll synthesis and has great popularization value.

The invention provides a compound for regulation and control of plant chlorophyll synthesis and a use thereof. The invention provides a protein-nucleic acid compound. A SCL27 protein is bonded to a specific area of a PORC gene so that the compound is formed. An experiment proves that the compound can regulate and control PORC gene expression, simply and effectively regulate and control plant chlorophyll synthesis and adjust plant chlorophyll content and blade color thereby controlling a leaf color or regulating plant photosynthesis efficiency.

The invention relates to a nitrogen-phosphorus-potassium compound fertilizer, which consists of the following raw materials in part by weight: 8-12% of nitrogen, 16-20% of phosphorous, 25-40% of potassium, 13-17% of medium elements and 1-3% of trace elements. By using the nitrogen-phosphorus-potassium compound fertilizer, not only can the requirements of a farm crop on lots of the nitrogen, the phosphorus and the potassium be met; a plant is impelled to grow, so that a plant body is tall and big, branches are thick and strong, stem leaves are luxuriant, blades are fat and big, and grains are full; but also a root system can be effectively promoted to be developed; the farm crop is accelerated to grow and develop; the performances of drought resistance, cold resistance, lodging resistance and resistance to diseases and insect pests are improved; fruits are mature ahead of time; furthermore, the trace elements such as ferrum, boron, arsenic, manganese, copper, cobalt, molybdenum and the like, which are needed for the growth of the plant, are replenished for soil; the synthesis and the stability of chlorophyll are promoted; an in-vivo redox reaction is participated; the biological nitrogen fixation and the development of a generative organ are promoted; and the yield of the farm crop can be further improved.

The invention provides a wheat low-temperature-resistant inducer product and a use method thereof. The efficient low-temperature-resistant inducer is prepared by compounding jasmonic acid methyl ester with earthworm active enzyme plant polypeptide liquid, urea, monopotassium phosphate and chitosan, and by spraying the low-temperature-resistant inducer on leaf surfaces, the chlorophyll synthesis of wheat leaves can be remarkably improved, and the damage of reactive oxygen free radicals and the like to plasma membranes is reduced; the activity of antioxidant enzymes and the accumulation of osmotic regulation substances are enhanced, so that the cold resistance of the plants is enhanced, and the damage of low-temperature stress to the wheat plants is relieved. The pH value of the low-temperature-resistant inducer provided by the invention is 5.5-6, and the low-temperature-resistant inducer can effectively reduce the pungent smell of a preparation and lower the requirements of the preparation on transportation and storage conditions.

The invention discloses a proliferation medium for raspberry tissue culture and a preparation method and application of the proliferation medium for the raspberry tissue culture. A basic medium is a modified MS medium, the modified MS medium is obtained by adjusting the concentration of magnesium sulfate heptahydrate, disodium edetate and ferrous sulfate heptahydrate in the MS medium. After beingadjusted, the concentration of magnesium sulfate heptahydrate is 720-760 mg / L, the concentration of disodium edetate is 70-80 mg / L, and the concentration of ferrous sulfate heptahydrate is 52-57 mg / L.The invention further provides the preparation method and application of the proliferation culture. According to the invention, the formula of the MS medium is improved, so that leaf colors are healthier, the chlorophyll synthesis is obviously doubled, the photosynthesis efficiency is obviously increased, and the seedling grows more healthily and strongly in the growth process of raspberries in the seedling stage to greatly improve the survival rates of transplanting and domesticating raspberry tissue culture seedlings in later stage.

The invention relates to the field of trace fertilizer, in particular to a magnesium-enriched foliar nutrient and application thereof in leaf vegetable planting. The magnesium-enriched foliar nutrient comprises 35-55 parts of water-soluble magnesium fertilizer, 20-40 parts of sugar alcohol magnesium, 2-4 parts of humic acid, 1-3 parts of nucleotide, 1-2 parts of jasmonic acid, 5-12 parts of plant growth promoter, 5-10 parts of auxiliary trace elements and 3-5 parts of surfactant. The magnesium-enriched foliar nutrient has the advantages that the magnesium-enriched foliar nutrient contains the fast-soluble magnesium fertilizer and the surfactant at the same time, considers both timeliness and absorption rate and is mainly applied to the leaf vegetable planting; the magnesium-enriched foliar nutrient is mainly used to solve the problem of magnesium shortage during the leaf vegetable planting and is especially applicable to sandy soil and acid soil; the surfactant is used to promote the absorption of the foliar nutrient, and high nutritional component permeability and high absorption rate are achieved; the magnesium needed by crops can be supplemented fast, chlorophyll synthesis is promoted, photosynthesis is enhanced evidently, crop nutritional growth is promoted, crop stress resistance is enhanced, and physiologic diseases such as leaf yellowing caused by the magnesium shortage can be prevented and relieved effectively.

The invention discloses a functional synergist for a fertilizer. The functional synergist comprises a component A and a component B; the component A comprises the following raw materials in parts by mass: 120-150 parts of a sugar alcohol solution, 50-80 parts of a polypeptide composition, 10-20 parts of a boron fertilizer, 10-20 parts of a zinc fertilizer, 2-10 parts of other medium trace element fertilizers and 20-100 parts of deionized water; the component B is a curing agent; the mass ratio of the component A to the component B is (23): 1. The invention also discloses application of the fertilizer functional synergist in fertilizers, including fertilizer coating, seed coating or foliage spraying. The fertilizer functional synergist provided by the invention can effectively improve the stress resistance and chlorophyll synthesis in the plant growth period, and improve the nutrient utilization rate of plants, thereby effectively improving the yield and quality of the plants.

The invention discloses a special fertilizer for preventing and controlling sugarcane manganese toxicity. The special fertilizer is mainly prepared from an iron fertilizer, a nitrogen fertilizer, boric acid, citric acid and a sulfur fertilizer. By spraying the special fertilizer for preventing and controlling sugarcane manganese toxicity disclosed by the invention, active iron lacking in sugarcane seedlings under stress of manganese toxicity is replenished in a timely manner, and nitrogen, magnesium, boron and sulfur nutrients of the seedlings are regulated, so as to promote chlorophyll synthesis to enable leaves to be green quickly. Through a synergist for inhibiting manganese transport while promoting the absorption and transport of other nutrients in the special fertilizer, the special fertilizer can give full play to nutritional effects thereof, so that the special fertilizer can quickly control the manganese toxicity in ratoon sugarcane, promote growth of seedlings, and increase the yield of cane stalks significantly. After the special fertilizer is applied for 1-3 weeks, the content of manganese in young leaves is decreased by 3.7%-50.9%; after the special fertilizer is applied for 7 days, the content of chlorophyll in young leaves of ratoon sugarcane is increased by more than 3 times, and the proportion of green leaves is increased by more than 5 times; after application, the stalk length during a sugarcane mature period is increased by 4.8% or above, the stalk diameter is increased by 3.9% or above, and the yield is increased by 18.8%-60.1%. The special fertilizer in solid state is convenient for transportation and storage, and easy in application method.

The invention specifically relates to a botanical insecticide and a preparation method thereof, belonging to the technical field of insect prevention of vegetable. The botanical insecticide is prepared from the following raw materials by weight: 8 to 12 parts of bitter tea seed meal, 10 to 15 parts of Rourea minor, 9 to 13 parts of Litsea glutinosa, 10 to 15 parts of Sassafras tzumu, 6 to 10 parts of diphenylurea sulfonic calcium, 5 to 9 parts of a penetrating agent and 5 to 9 parts of a modified starch solution. The botanical insecticide is prepared from natural plants, has the advantages of healthiness, no toxicity and no pollution, exerts good insecticidal effect, and can promote chlorophyll synthesis and cell division of vegetable, so yield increase effect is obtained; thus, the botanical insecticide has good promotion value.

The invention discloses application of a prepared domestic waste compost composite bacterial agent to enhancing photosynthetic capacity of lawn plants and promoting synthesis of chlorophyll. The composite bacterial agent consists of three strains, namely bacillus subtilis, actinomycetes and saccharomycetes in volume ratio of 1 to 1 to 1. Experiments show that seed germination rate, seedling height, aboveground dry weight, root weight, chlorophyll a content and chlorophyll b content of festuca arundinacea are 13.41 percent, 58.92 percent, 72.86 percent, 100 percent, 68.92 percent and 37.61 percent higher than contrast values respectively and the seed germination rate, the seedling height, the aboveground dry weight, the root weight, the chlorophyll a content and the chlorophyll b content of ryegrass are 15.11 percent, 34.44 percent, 71.74 percent, 100 percent, 66.89 percent and 33.41 percent higher than contrast values respectively after being treated by inoculating the three-strain composite bacterial agent. Technical effects show that the high-efficiency compost composite bacterial agent provides proper growth conditions and nutrients for plants, meets requirements on growth conditions required by the plants and therefore contributes to growth of the ryegrass and the festuca arundinacea.

The invention provides an inducing coloring agent for bagged peach fruits in rain sheltering facilities. The inducing coloring agent comprises S-abscisic acid and / or brassinolide, prohexadione calcium and other ingredients, the sum of the S-abscisic acid and / or brassinolide and prohexadione calcium accounts for 1-40 wt% of the coloring agent, and the weight ratio of the sum of the S-abscisic acid and / or brassinolide to the prohexadione calcium is 1:(5-10). In a new shoot vigorous growth period, a fruit swelling period and after picking of the peach trees, other ingredients are wood vinegar, jasmonic acid compounds or isosorbide respectively. The inducing coloring agent with different compounding formulas can be adopted according to the growth condition of peach fruits, meanwhile, antagonism and synergistic effects of the compound components are provided, the chlorophyll synthesis and degradation speed is balanced, the content of anthocyanin and carotenoid in pericarp is increased, the cell division speed is increased while cell division is promoted, and therefore, the fruit is bright in appearance and color.

A planting method for increasing pepper production, characterized in that it comprises the following steps: (1) land selection; (2) seed treatment; (3) germination and sowing; (4) seedling cultivation; (5) transplanting and planting; (6) ) Topdressing during the growth period; (7) Fruit ripening. The pepper planting method of the present invention applies base fertilizer treatment to the land, provides a large amount of organic substances and trace elements for the development of pepper, and improves the environment of the soil; when germination is sown, the seeds are sterilized and treated with ammonia-resistant nitrogen-fixing bacteria solution after seed soaking, Greatly strengthened the physique of the seedlings, promoted the germination potential of the seedlings, and improved the disease resistance; the potassium dihydrogen phosphate and potassium chloride solution applied at the seedling cultivation stage together promoted the synthesis of chlorophyll, and reasonable topdressing in the later stage improved the growth of peppers. quality. The planting method is scientific, not only improves the yield of pepper but also ensures the quality of pepper, and has high economic value.

The invention discloses a fruit swelling and coloring microbial agent which is prepared from the following raw materials in percentage by weight: 45-50% of enzymolysis soybean phospholipid liquid, 8-12% of monopotassium phosphate, 0.4-0.6% of water-soluble bacillus subtilis powder, 8-12% of amino acid liquid, 15-25% of biochemical potassium fulvate, 1.4-2.6% of polyglutamic acid and 7-13% of brownsugar. The raw materials are mixed in percentage by weight to obtain the fruit swelling and coloring microbial agent. The enzymolysis soybean phospholipid is obtained by adding various enzymes into soybean phospholipid liquid for enzymolysis, the enzymolysis soybean phospholipid prepared by the invention is low in cost and can provide continuous nutrients for bacillus subtilis, and the enzymolysis soybean phospholipid is used as a main raw material, so that the prepared microbial agent is beneficial to fruit swelling and coloring of crops; the crop quality is improved; and chlorophyll synthesis is enhanced, crop growth is facilitated, and sugar accumulation is increased.