Fruit swelling and coloring microbial agent
A technology of microbial inoculum and fruit expansion, applied in the direction of fertilizer mixture, application, nitrogen fertilizer, etc., can solve the problems of nutrient waste of added type and amount, high fertilizer cost, etc., and achieve the goal of increasing VC content, improving soil environment, and increasing sugar accumulation Effect
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Embodiment 1
[0038] Embodiment 1: the preparation of soybean lecithin liquid
[0039] 1) Take 1000g of soybean oil bottoms, filter under reduced pressure at room temperature to remove most of the water, and then use a rotary evaporator to further concentrate and evaporate the water at a temperature of 80°C to obtain crude phospholipids.
[0040] 2) Get 600g of crude phospholipids and add it to a 2000ml Erlenmeyer flask, add 600g of acetone, and stir for 4h at a temperature of 25°C, static layering, separation of solid and liquid, suction filtration under reduced pressure, recovery of acetone liquid, and the obtained crude phospholipid powder. Vacuum dry.
[0041] 3) Put 500 g of crude phospholipid powder in a 2000 ml Erlenmeyer flask, add 5 g of 30% hydrogen peroxide by mass fraction, stir at 80° C. for 2 hours, and steam the hydrogen peroxide to obtain refined soybean lecithin.
[0042] 4) Dissolving 250 g of refined soy lecithin in 15 g of soybean oil and 10 g of corn oil to obtain a so...
Embodiment 2
[0043]Embodiment 2: Preparation of enzymatic hydrolysis soybean lecithin liquid
[0044] Take 250g of the soybean lecithin liquid prepared in Example 1, add 1.0g of catalase, 0.75g of pepsin and 0.75g of urease and adjust the pH to 7.0, and the enzymolysis temperature is 25°C. After 48 hours of enzymolysis, heat to 80°C Stop the enzymatic hydrolysis reaction to obtain the enzymatic hydrolysis soybean lecithin liquid.
Embodiment 3
[0045] Embodiment 3: the preparation of expanded fruit colored microbial inoculum
[0046] Get 47.5g of the enzymolysis soybean lecithin liquid prepared in Example 2 and mix with 10g of potassium dihydrogen phosphate, 0.5g of water-soluble Bacillus subtilis powder, 10 amino acid liquids, 20g of biochemical potassium fulvic acid, 2g of polyglutamic acid, and 10g of brown sugar and stir evenly to obtain 100 g of expanded fruit coloring microbial inoculum.
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